山月桂组培苗生根实验
详细信息 查看官网全文
- 英文论文题名：Rooting of In Vitro Seedling of Kalmia latifolia
- 论文作者：周穆杰 ; 邢文 ; 刘彩贤
- 英文论文作者：ZHOU Mu-jie ; XING Wen ; LIU Cai-xian ; Department of Landscape Architecture ; Central South University of Forestry and Technology
- 年：2016
- 作者机构：中南林业科技大学风景园林学院;
- 论文关键词：山月桂 ; 组织培养 ; 生根 ; 移栽
- 英文论文关键词：Kalmia latifolia ‘Olympic Fire’ ; Tissue cultured plant ; Medium ; Rooting ; Transplanting
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S685.99
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：5
- 文件大小：925k
- 原文格式：O
- 会议级别：全国
摘要
本实验以山月桂‘奥运圣火'组培苗为材料,研究不同植物生长调节剂和凝固剂对其生根的影响,同时研究不同栽培基质对其移栽成活率的影响,为其将来实现工厂化育苗提供依据。结果表明:①同时添加IBA和2-ip更有助于山月桂‘奥运圣火'组培苗生根,并且珍珠岩比琼脂更适合做生根培养的凝固剂,因此最适合的生根培养基为:1/2 WPM+1.0mg·L~(-1)IBA+0.1mg·L~(-1)2-ip+珍珠岩,生根率87.32%,平均根长为4.39cm;②适合组培苗的移栽基质为珍珠岩、泥炭土(1:1,V/V)的混合基质,成活率为46.67%。
In the research,we took the Kalmia.latifolia‘Olympic Fire' as material to study the effects of different plant growth regulator and coagulant on the rooting of plantlet in vitro,as well as the effects of different substrates on the transplanting of tissue cultured seedling.The results showed that;①adding the IBA and 2-ip simultaneously in the culture medium would induce a definite improvement on the rooting of seedlings,and the perlite was more suited to be the coagulant than the agar in the rooting medium.As a consequent,the best medium for rooting of plantlet was 1/2WPM+1.0mg·L~(-1)IBA+0.1mg·L~(-1) 2-ip+perlite,with the rooting rate of 87.32%and the average root length of 4.39cm;②The most suitable media for transplanting was the mixture of perlite and peat soil with the volume ratio of 1:1,and the survival rate was 46.67%.
In the research,we took the Kalmia.latifolia‘Olympic Fire' as material to study the effects of different plant growth regulator and coagulant on the rooting of plantlet in vitro,as well as the effects of different substrates on the transplanting of tissue cultured seedling.The results showed that;①adding the IBA and 2-ip simultaneously in the culture medium would induce a definite improvement on the rooting of seedlings,and the perlite was more suited to be the coagulant than the agar in the rooting medium.As a consequent,the best medium for rooting of plantlet was 1/2WPM+1.0mg·L~(-1)IBA+0.1mg·L~(-1) 2-ip+perlite,with the rooting rate of 87.32%and the average root length of 4.39cm;②The most suitable media for transplanting was the mixture of perlite and peat soil with the volume ratio of 1:1,and the survival rate was 46.67%.
引文
1.Buttrick R L Connecticut S state flower,the mountain laurel,a forest plant[M].Marsh Botanic Garden Publication(Yale University),1924,1:1-28.
2.杜丽娜.不同基因型花楸的生根能力的比较及生理生化的测定[D].辽宁师范大学硕士学位论文,2007:49-50.
3.Fordham D.Production of plants from seed.Combined Proceedings[J].International Plant Propagators'Society,1976,26:139-145.
4.Kevers C,Menard D,Marchand S,et al.Rooting in vitro and ex vitro and behaviour at acclimatization of Kalmia and Rhododendron propagated in vitro[J].Mededelingen van de Faculteit Landbouwwetenschappen,Rijksuniversiteit Gent.1990,55:1267-1273.
5.Laycock W A.Distribution of roots and rhizomes in different soil types in the Pine Barrens of New Jersey.Geological Survey Professional Paper 563-C.U.S.Department of the Interior,Geological Survey,Washington,D C,1967.
6.Leo J H,Gould J H.in vitro shoot-tip grafting improves recovery of cotton plants from Culture[J].Plant Cell Tissue Org Culture,1999,57(3):211-213.
7.李何.山月桂应用现状及扦插繁殖技术[D].长沙:中南林业科技大学,2014.
8.李泽,谭晓风,袁军,等.油茶良种‘华硕'的组织培养及高效生根[J].植物生理学报,2014,50(11):1721-1726.
9.林玉玲.4个品种长寿花试管苗的增殖与生根培养[J].热带作物学报,2007(2):80-86.
10.刘翠琼,汤浩茹,罗娅,等.不同基因型梨叶片离体培养和植株再生[J].园艺学报,2005,32(6):1080-1083.
11.Nishimura T,Hasegawa S,Meguro A,et al.Micropropagation technique for mountain laurel(Kalmia latifolia L.)using cytokinin and growth retardant[J].Japanese Journal of Crop Science,2004,73(1):107-113.
12.Pati P K,Rath S P,Sharma M,et al.In vitro propagation of rose—a review[J].Biotechnology Advances,2005,24(1):94-114.
13.Tibbits WN,White T L,Hodge G R,et al.Genetic control of rooting ability of stem cuttings in Eucalyptus nitens[J].Australian Journal of Botany,1997,45(1):203-210.
14.孙清荣,孙洪雁,刘庆忠,等.不同倍性苹果叶片不定植株再生研究[J].落叶果树,2000(2):9-11.
15.汤浩茹,刘翠琼,罗娅,等.培养基和培养条件对4个梨基因型试管苗生根的影响[J].果树学报,2006,23(2):283-286.
16.周艳.山月桂的引种实验研究[D].贵阳:贵州师范大学,2014.
2.杜丽娜.不同基因型花楸的生根能力的比较及生理生化的测定[D].辽宁师范大学硕士学位论文,2007:49-50.
3.Fordham D.Production of plants from seed.Combined Proceedings[J].International Plant Propagators'Society,1976,26:139-145.
4.Kevers C,Menard D,Marchand S,et al.Rooting in vitro and ex vitro and behaviour at acclimatization of Kalmia and Rhododendron propagated in vitro[J].Mededelingen van de Faculteit Landbouwwetenschappen,Rijksuniversiteit Gent.1990,55:1267-1273.
5.Laycock W A.Distribution of roots and rhizomes in different soil types in the Pine Barrens of New Jersey.Geological Survey Professional Paper 563-C.U.S.Department of the Interior,Geological Survey,Washington,D C,1967.
6.Leo J H,Gould J H.in vitro shoot-tip grafting improves recovery of cotton plants from Culture[J].Plant Cell Tissue Org Culture,1999,57(3):211-213.
7.李何.山月桂应用现状及扦插繁殖技术[D].长沙:中南林业科技大学,2014.
8.李泽,谭晓风,袁军,等.油茶良种‘华硕'的组织培养及高效生根[J].植物生理学报,2014,50(11):1721-1726.
9.林玉玲.4个品种长寿花试管苗的增殖与生根培养[J].热带作物学报,2007(2):80-86.
10.刘翠琼,汤浩茹,罗娅,等.不同基因型梨叶片离体培养和植株再生[J].园艺学报,2005,32(6):1080-1083.
11.Nishimura T,Hasegawa S,Meguro A,et al.Micropropagation technique for mountain laurel(Kalmia latifolia L.)using cytokinin and growth retardant[J].Japanese Journal of Crop Science,2004,73(1):107-113.
12.Pati P K,Rath S P,Sharma M,et al.In vitro propagation of rose—a review[J].Biotechnology Advances,2005,24(1):94-114.
13.Tibbits WN,White T L,Hodge G R,et al.Genetic control of rooting ability of stem cuttings in Eucalyptus nitens[J].Australian Journal of Botany,1997,45(1):203-210.
14.孙清荣,孙洪雁,刘庆忠,等.不同倍性苹果叶片不定植株再生研究[J].落叶果树,2000(2):9-11.
15.汤浩茹,刘翠琼,罗娅,等.培养基和培养条件对4个梨基因型试管苗生根的影响[J].果树学报,2006,23(2):283-286.
16.周艳.山月桂的引种实验研究[D].贵阳:贵州师范大学,2014.