驻极体外电场对季铵化壳聚糖生长因子纳米粒制备的影响
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- 英文论文题名：The effect of the preparation of chitosan nanopaticles by electret electrostatic-field
- 论文作者：郭鑫 ; 石岩 ; 梁媛媛 ; 黄平 ; 崔黎丽 ; 江键
- 英文论文作者：Guo Xin ; Shi Yan ; Liang Yuanyuan ; Huangping ; Cui Lili ; Jiang Jian ; Department of Physics and Mathematics ; Second Military Medical University ; The medical department ; Second Military Medical University ; Department of Inorganic Chemistry ; Second Military Medical University
- 年：2015
- 作者机构：第二军医大学基础部数理教研室;第二军医大学海医系;第二军医大学药学院无机教研室;
- 论文关键词：驻极体 ; 静电场 ; 碱性生长因子 ; 纳米粒 ; 季铵化壳聚糖
- 英文论文关键词：electret electrostatic field ; EGF ; nanoparticles ; chitosan
- 会议召开时间：2015-08-12
- 会议录名称：静电放电：从地面新技术应用到空间卫星安全防护—中国物理学会第二十届全国静电学术会议论文集
- 语种：中文
- 分类号：O636.1;TQ460.1
- 学会代码：WLJD
- 会议名称：静电放电 ; 从地面新技术应用到空间卫星安全防护—中国物理学会第二十届全国静电学术会议
- 会议地点：中国河北石家庄
- 主办单位：中国物理学会静电专业委员会
- 学会名称：中国物理学会静电专业委员会
- 页数：5
- 文件大小：449k
- 原文格式：D
- 会议级别：全国
摘要
为研究静电场对生长因子纳米粒制备及表征的影响,进一步探究壳聚糖生长因子纳米粒的经皮载药转运机制,本文以碱性生长因子为模型药物,以季铵化壳聚糖作为材料,以三聚磷酸钠作为交联剂,在利用不同表面电位驻极体产生的外静电场下,采用离子交联法制备不同场强电场处理下的载碱性生长因子的季铵化壳聚糖纳米粒溶液。借助于透射电镜观察各种条件下制备纳米粒的表面形态,利用粒径/Zeta电位仪测定纳米粒的粒径和Zeta电位。研究结果显示:(1)用离子交联法制备的季铵化壳聚糖生长因子纳米粒呈球形,分布均匀,分散性较好,且具有良好的稳定性。(2)外静电场能引起壳聚糖生长因子纳米粒极化。(3)壳聚糖生长因子纳米粒的粒径值随静电场场强的增大而减小,zeta电位的变化与外加电场的极性密切相关。(4)经电场处理后的壳聚糖生长因子纳米粒的粒径随存放时间而减小。结论:壳聚糖生长因子纳米粒的粒径和表面性征与外加电场的场强大小和极性密切相关,要制备优质纳米粒,必须合理选择电场参数。
To the study of the influence of preparation and characterization of EGF nanoparticles and explore the percutaneous transfer mechanism of drug-loading nanoparticles.In this paper,Based on the basic EGF as model drug,quaternary ammonium chitosan as material,sodium tripolyphosphate as crosslinking agent,using the electret electrostatic field to Prepare EGF-loading chitosan nanoparticle solution.By means of transmission electron microscope to observe the surface morphology under different conditions.The results showed that:(1)The EGF-loading chitosan nanoparticles has uniform distribution,good dispersibility and stability.(2)The EGF can be polarized by electrostatic field.(3)The particle size of EGF-loading chitosan nanoparticles decreases with the intensity of the electrostatic field;the change of the zeta potential is closely related to the polarity of the applied electric field.(4)After the electric field treatment of chitosan nanoparticles,its particle size decreases with storage time.Result:The size and surface properties of chitosan nanoparticles is closely related to the applied electric field strength and polarity,to prepare the good quality chitosan nanoparticles,must choose a reasonable electric field parameters.
To the study of the influence of preparation and characterization of EGF nanoparticles and explore the percutaneous transfer mechanism of drug-loading nanoparticles.In this paper,Based on the basic EGF as model drug,quaternary ammonium chitosan as material,sodium tripolyphosphate as crosslinking agent,using the electret electrostatic field to Prepare EGF-loading chitosan nanoparticle solution.By means of transmission electron microscope to observe the surface morphology under different conditions.The results showed that:(1)The EGF-loading chitosan nanoparticles has uniform distribution,good dispersibility and stability.(2)The EGF can be polarized by electrostatic field.(3)The particle size of EGF-loading chitosan nanoparticles decreases with the intensity of the electrostatic field;the change of the zeta potential is closely related to the polarity of the applied electric field.(4)After the electric field treatment of chitosan nanoparticles,its particle size decreases with storage time.Result:The size and surface properties of chitosan nanoparticles is closely related to the applied electric field strength and polarity,to prepare the good quality chitosan nanoparticles,must choose a reasonable electric field parameters.
引文
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[8]Xin Guo,Yuan-yuan Liang,Hong-yue Liu,Li-li Cui,Jian Jiang*,The study of electrostatic andpiezoelectric properties of positive electret cyclosporine A patch,Journal of Physics:Conference Series,2013,418,012149.
[9]Li-li Cui,Lin Ma,Yuan-yuan Liang,Hong-yue Liu,Xin Guo,Jian Jiang*Enhancing effect of negative PPelectret on transdermal delivery of cyclosporine Asolution in vitro and its synergistic effect with ethyl oleate,Journal of Physics:Conference Series,2013,418,012148.
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[11]Jian Jiang,Yuan-yuan Liang,Fa-jie Dong,Lin Ma,Hong-yue Liu,Li-li Cui,Penetration and stability of external electrostatic field of PP electret through skin,IEEE T Dielect El In,2012,19(4):1137-1142.
[2]J Michael Sorrell,Arnold I Caplan.Topical delivery of mesenchymal stem cells and their function in wounds.Sorrell and Caplan Stem Cell Research&Therapy 2010,1(30):1-6
[3]冯永强等,电场对创面愈合的影响.中国美容医学.2008,17(10):1550-1552
[4]Nuccitelli R Nuccitelli P,Ramlatchan s’et a Llmaging the electric field associated with mouse and human skin wounds.Wound Repair Regen,2008,16(3):432-441.
[5]欧歌,廖德华,张凯,戴伟,向大熊.采用离子交联法制备胰岛素纳米粒的研究进展.中南药学.2013,11(2):116-120.
[6]任慧梅,曾令冰,曾明.壳聚糖纳米粒作为疫苗佐剂的研究进展.中国生物制品学杂志.2012.25(6):786-788
[7]李典,徐若诗,徐小平.壳聚糖微/纳米粒子的制备及其在黏膜给药中的应用.华西药学杂志.2014,29(2):219-221.
[8]Xin Guo,Yuan-yuan Liang,Hong-yue Liu,Li-li Cui,Jian Jiang*,The study of electrostatic andpiezoelectric properties of positive electret cyclosporine A patch,Journal of Physics:Conference Series,2013,418,012149.
[9]Li-li Cui,Lin Ma,Yuan-yuan Liang,Hong-yue Liu,Xin Guo,Jian Jiang*Enhancing effect of negative PPelectret on transdermal delivery of cyclosporine Asolution in vitro and its synergistic effect with ethyl oleate,Journal of Physics:Conference Series,2013,418,012148.
[10]LL Cui,YY Liang,L Ma,Xin Guo,HY Liu,JJiang*,The combinational effect of PP electret and monoolein on transdermal delivery of cyclosporine A in vitro,Journal of Electrostatics,2013,224-227.
[11]Jian Jiang,Yuan-yuan Liang,Fa-jie Dong,Lin Ma,Hong-yue Liu,Li-li Cui,Penetration and stability of external electrostatic field of PP electret through skin,IEEE T Dielect El In,2012,19(4):1137-1142.