针刺结合美多巴对帕金森小鼠模型多巴胺能神经元及脑线粒体复合物酶的影响
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- 英文论文题名：The effects of Acupuncture combined with madopar on dopaminergic neurons and brain mitochondria complex enzyme in Parkinson's disease mouse model
- 论文作者：马浩洁 ; 王媛媛 ; 盖聪 ; 强天遥 ; 冯琬迪 ; 柴原 ; 郭振宇 ; 孙红梅
- 英文论文作者：MA Hao-Jie ; WANG Yuan-Yuan ; GAI Cong ; QIANG Tian-Yao ; FENG Wan-Di ; CHAI Yuan ; GUO Zhen-Yu ; SUN Hong-Mei ; Department of Anatomy ; Beijing university of Chinese medicine
- 年：2017
- 作者机构：北京中医药大学解剖教研室;
- 论文关键词：帕金森病 ; MPTP ; 多巴胺能神经元 ; 线粒体复合物
- 英文论文关键词：Parkinson' s disease ; MPTP ; Dopaminergic neurons ; Mitochondrial complex
- 会议召开时间：2017-08-07
- 会议录名称：中国解剖学会第六届全国解剖学技术学术会议论文汇编
- 语种：中文
- 分类号：R-332;R742.5
- 学会代码：ZGJP
- 会议名称：中国解剖学会第六届全国解剖学技术学术会议
- 会议地点：中国江西九江
- 主办单位：中国解剖学会科技开发和咨询工作委员会
- 学会名称：中国解剖学会
- 页数：5
- 文件大小：2458k
- 原文格式：D
- 会议级别：全国
摘要
目的探讨针刺百会和大椎穴对帕金森病(PD)小鼠多巴胺能(DA)神经元的保护作用及可能机制。方法 C57BL/6雄性小鼠随机分为5组:正常组、模型组、针刺组、美多巴组、针刺结合美多巴组。应用腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)建立PD动物模型,造模后动物存活56天。采用免疫组织化学技术检测黑质酪氨酸羟化酶(TH)阳性神经元数目,透射电镜观察其神经元的超微结构,并测定各组小鼠脑线粒体复合物的活性。结果与正常组相比,模型组TH阳性神经元数目较正常组减少(P<0.05),针刺组、针刺结合美多巴组较模型组明显增多(P<0.05)。电镜观察下发现模型组神经元有不同程度的损伤,各治疗组神经元损伤有所减轻,针刺结合美多巴组神经元损伤最轻。与正常组相比,模型组小鼠线粒体复合物活性均显著下降(P<0.05);与模型组相比,针刺组线粒体复合物Ⅱ、Ⅲ活性,针刺结合美多巴组线粒体复合物Ⅰ、Ⅳ活性显著上升(P<0.05)。结论针刺百会、大椎穴可能通过抑制脑内线粒体复合物活性的下降来维持其线粒体的功能,以达到保护多巴胺能神经元活性的目的。
Objective To investigate the mechanisms of acupuncture in preventing and treating Parkinson's disease(PD).Methods Male C57BL/6 mice were randomly divided into five groups:control group(C),model group(M),acupuncture group(A),Medopar group(P),acupuncture mixed with Medopar group(AP).The PD model was induced by MPTP with intraperitoneal injection.Different treatments were taken.All mice were sacrificed at the 56 th day respectively after first injecting with MPTP.The TH+(Tyrosine hydroxylase) dopaminergic neurons in the substantia nigra were counted in every group by immunohistochemistry technique.The ultrastructure of neurons and photography were observed by transmission electron microscope.The activity of mitochondrial complex in the brain of mice was measured respectively.Results The number of TH positive neurons in M was much less than that in C(P<0.05).The numbers of the neurons in A and AP were obviously more than that in M(P<0.05).The dopaminergic neurons in M were found damaged more than that in C,the dopaminergic neurons in A,P,AP were found damaged less than that in M,the dopaminergic neurons in AP had the lightest minor damage.The activities of mitochondrial complex Ⅰ,Ⅱ,Ⅲ and Ⅳ in M were significantly lower(P<0.05)when compared with those in C;the activities of mitochondrial complexⅡ,Ⅲ in A andⅠ,Ⅳ in AP were significantly higher(P<0.05)compared with those in the M.Conclusions Needling Baihui point and Dazhui point can inhibit the activity drop of brain mitochondria complex to maintain mitochondrial function in order to protect dopaminergic neurons.
Objective To investigate the mechanisms of acupuncture in preventing and treating Parkinson's disease(PD).Methods Male C57BL/6 mice were randomly divided into five groups:control group(C),model group(M),acupuncture group(A),Medopar group(P),acupuncture mixed with Medopar group(AP).The PD model was induced by MPTP with intraperitoneal injection.Different treatments were taken.All mice were sacrificed at the 56 th day respectively after first injecting with MPTP.The TH+(Tyrosine hydroxylase) dopaminergic neurons in the substantia nigra were counted in every group by immunohistochemistry technique.The ultrastructure of neurons and photography were observed by transmission electron microscope.The activity of mitochondrial complex in the brain of mice was measured respectively.Results The number of TH positive neurons in M was much less than that in C(P<0.05).The numbers of the neurons in A and AP were obviously more than that in M(P<0.05).The dopaminergic neurons in M were found damaged more than that in C,the dopaminergic neurons in A,P,AP were found damaged less than that in M,the dopaminergic neurons in AP had the lightest minor damage.The activities of mitochondrial complex Ⅰ,Ⅱ,Ⅲ and Ⅳ in M were significantly lower(P<0.05)when compared with those in C;the activities of mitochondrial complexⅡ,Ⅲ in A andⅠ,Ⅳ in AP were significantly higher(P<0.05)compared with those in the M.Conclusions Needling Baihui point and Dazhui point can inhibit the activity drop of brain mitochondria complex to maintain mitochondrial function in order to protect dopaminergic neurons.
引文
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[2]郭涌斐,孙懿,赵欣,等.DJ-1蛋白对线粒体的功能调节在帕金森病中的作用[J].中国药理学通报,2016,32(1):22-26.
[3]姜文霞,张新化,顾晓松.帕金森病的发病机制和治疗研究进展[J].交通医学,2016,30(1):1-7.
[4]孙红梅,吴海霞,许红,等.针刺督脉穴对帕金森病小鼠多巴胺能神经元保护及超微结构的影响[J].北京中医药大学学报,2010,33(4):257-261.
[5]何建成,王文武,丁宏娟,等.50 mg/kg剂量左旋多巴对帕金森病大鼠神经行为学和超微结构的影响[J].中国老年学杂志,2010,30(9):1209-1212.
[6]吴江.神经病学[M].2版.北京:人民卫生出版社,2011:259-260.
[7]邹勇.针刺治疗帕金森病的临床观察[J].上海针灸杂志,2006,25(1):27-28.
[8]Blandini F,Armentero M T.Animal models of Parkinson's dis-ease[J].FEBS J,2012,279(7):1156.
[9]朱会杰,曹林,唐波,等.熄风汤对帕金森病模型小鼠多巴胺能神经元的影响[J].东南大学学报,2016,35(1):11-16.
[10]刘翠芳,陈文强,王宁群,等.帕金森病的发病机制及药物治疗研究现[J].北京中医药,2016,35(5):436-439.
[11]孙红梅,白丽敏,张军,等.银杏平颤方及其拆方对帕金森病模型小鼠脑内线粒体酶复合体活性的影响[J].中国中西医结合杂志,2005,25(11):1008-1011.
[12]孙红梅,和欣,王媛媛,等.针刺百会、大椎穴对帕金森病小鼠脑线粒体复合物活性的影响[J].北京中医药大学学报,2011,34(4):250-253.