分散固相萃取结合亲水作用色谱-串联质谱法检测饲料中7种抗病毒药物含量
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- 英文论文题名：Determination of 7 Antivirus in Feed by QuCHERS Combined with Hydrophilic Interaction Chromatography-Tandem Mass Spectrometry
- 论文作者：吴剑平 ; 张鑫 ; 李丹妮 ; 严凤 ; 潘娟 ; 张婧
- 英文论文作者：WU Jian-ping ; ZHANG Xin ; LI Dan-ni ; YAN Feng ; PAN Juan ; Zhang Jing ; Shanghai Municipal Supervisory Institute Veterinary Drugs and Feedstaff
- 年：2016
- 作者机构：上海市兽药饲料检测所;
- 论文关键词：抗病毒药物 ; 分散固相萃取 ; 亲水作用色谱 ; 串联质谱法
- 英文论文关键词：7 antivirus ; QuCHERS ; HILIC ; Tandem mass spectrometry
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S816.17
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：9
- 文件大小：1032k
- 原文格式：O
- 会议级别：全国
摘要
建立了同时检测饲料中7种抗病毒药物含量的分散固相萃取(QuCHERS)结合亲水作用色谱(HILIC)-串联质谱检测方法,包括了金刚烷胺、吗啉胍、金刚乙胺、阿昔洛韦、利巴韦林、更昔洛韦和奥司他韦。选择使用乙腈提取,无水MgSO_4进行脱水和盐析,C18粉和丙基乙二胺(Primary secondary amine,PSA)进行样品净化。使用亲水作用液相色谱柱进行分离,流动相为0.2%甲酸和乙腈,采用梯度洗脱,三重四级杆质谱进行定性定量分析。实验结果表明,方法的最低检出限为25μg/kg,最低定量限为50μg/kg,在50~5000μg/kg范围内线性关系良好(r>0.995),添加回收率在60%~110%,RSD<10%,表明该方法具有较好的准确度与精密度。
To determine 7 antivirus in feed include amantadine,moroxydine,rimantadine,acyclovir,ribavirin,ganciclovir and oseltamivir,a QuCHERS combined with hydrophilic interaction chromatography(HILIC)- tandem mass method was established.The acetonitrilewas chosen to extract the target compounds,the anhydrous magnesium sulfate was chosen to dehydrate and salt out,and the C18 powder and the primary secondary amine(PSA) were chosen to purify the sample.After centrifugation,the extract was determined by the method below.The targets wasseparated bythe gradient elution with a HILIC column as the stationary phaseand0.2%formic acid with acetonitrile as the mobile phase.The qualification and quantification were achieved by a tandem mass spectrometry.The results showed that the LOD of the method was 25μg/kg and the LOQ was 50μg/kg.The linearity of the method was good in the range of 50 ~ 5000μg/kgand the liner correlations wereall above 0.995,and the recovery of 7 targets werebetween60%~110%,and the RSD were lower than 10%,which showed the good accuracy and precision of the method.
To determine 7 antivirus in feed include amantadine,moroxydine,rimantadine,acyclovir,ribavirin,ganciclovir and oseltamivir,a QuCHERS combined with hydrophilic interaction chromatography(HILIC)- tandem mass method was established.The acetonitrilewas chosen to extract the target compounds,the anhydrous magnesium sulfate was chosen to dehydrate and salt out,and the C18 powder and the primary secondary amine(PSA) were chosen to purify the sample.After centrifugation,the extract was determined by the method below.The targets wasseparated bythe gradient elution with a HILIC column as the stationary phaseand0.2%formic acid with acetonitrile as the mobile phase.The qualification and quantification were achieved by a tandem mass spectrometry.The results showed that the LOD of the method was 25μg/kg and the LOQ was 50μg/kg.The linearity of the method was good in the range of 50 ~ 5000μg/kgand the liner correlations wereall above 0.995,and the recovery of 7 targets werebetween60%~110%,and the RSD were lower than 10%,which showed the good accuracy and precision of the method.
引文
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[7]陈慧华,韦敏珏,周炜,等.液相色谱-串联质谱法测定动物组织中金刚烷胺和金刚乙胺的残留量[J].质谱学报,2013,34(04):227~232.
[8]张鑫,吴剑平,严凤,等.基于双柱固相萃取-色质联用技术同时检测鸡肉中8种抗病毒药物残留[J].中国兽药杂志,49(09):45~50.
[9]周晓,张勋,康明琴,等.固相萃取净化-液相色谱串联质谱法测定饲料中金刚烷胺含量[J].饲料检测,2014,1(01):55~59.
[10]艾连峰,马育松,陈瑞春,等.在线净化液相色谱串联质谱法测定动物源食品中金刚烷胺的残留[J].分析化学,2013,41(08):1194~1198.
[2]顾欣,张鑫,李丹妮,等.我国兽用抗菌药物使用现况及“无抗”饲养的探讨[J].中国兽药杂志,2013,47(8):54~57.
[3]李振,王云建.畜禽养殖中抗生素使用的现状、问题及对策[J].今日畜牧兽医,2009,24(08):1~3.
[4]沈林凤.滥用抗病毒药物对患者危害和临床治疗分析[J].医学信息,2011,24(06):3816.
[5]李必会.畜牧生产中常用的抗病毒药物及作用机制[J].浙江畜牧兽医,2014,1(01):35.
[6]杨泽林,吴宣,岳华,等.抗病毒药物在兽医上的应用与存在的问题[J].四川畜牧兽医,2014,30(05):31~32.
[7]陈慧华,韦敏珏,周炜,等.液相色谱-串联质谱法测定动物组织中金刚烷胺和金刚乙胺的残留量[J].质谱学报,2013,34(04):227~232.
[8]张鑫,吴剑平,严凤,等.基于双柱固相萃取-色质联用技术同时检测鸡肉中8种抗病毒药物残留[J].中国兽药杂志,49(09):45~50.
[9]周晓,张勋,康明琴,等.固相萃取净化-液相色谱串联质谱法测定饲料中金刚烷胺含量[J].饲料检测,2014,1(01):55~59.
[10]艾连峰,马育松,陈瑞春,等.在线净化液相色谱串联质谱法测定动物源食品中金刚烷胺的残留[J].分析化学,2013,41(08):1194~1198.
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