复合聚酯纳米探针在靶向叶酸受体的活体光声成像中的应用
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- 英文论文题名：Hybrid Polymeric Nanoprobes for Folate Receptor-Targeted Photoacoustic Imaging In Vivo
- 论文作者：刘哲 ; 李宜鸿 ; 宋远会 ; 徐齐恩
- 英文论文作者：Zhe Liu ; Yihong Li ; Yuanhui Song ; Qien Xu ; Wenzhou Institute of Biomaterials and Engineering ; Chinese Academy of Sciences ; Wenzhou Institut of Biomaterials and Engineering ; Wenzhou Medical University
- 年：2016
- 作者机构：中科院温州生物材料与工程研究所(筹);温州生物材料与工程研究所温州医科大学;
- 论文关键词：纳米探针 ; 光声成像 ; 叶酸受体 ; 乳腺癌
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十八分会：化学生物学
- 语种：中文
- 分类号：O631.3;TB383.1
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十八分会 ; 化学生物学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：250k
- 原文格式：D
- 会议级别：全国
摘要
光声成像是一种非侵入性、非离子化的生物影像模式,它既结合了声学成像穿透深、分辨率高的优点,也结合了光学成像灵敏度高的优势。构建生物相容的光声成像探针并应用于疾病的早期诊断,对于疾病的确诊与病患的治愈具有重要意义。我们通过乳化聚合、亚甲蓝包被、表面修饰等方法构建出了叶酸受体靶向的复合聚酯纳米探针,通过电镜、红外、核磁与光电子能谱等手段对纳米探针进行化学表征。对非靶向和靶向探针分别进行细胞实验,结合流式细胞术定量分析探针寻靶能力和效果。在体内光声成像实验中,分别以MDAMB231与U87MG作为叶酸受体阳性、阴性对照,通过瘤周注射探针进行活体成像。结果表明,聚酯光声纳米探针对叶酸过度表达的MDAMB231细胞具有良好的寻靶能力,可以有效地增强光声信号对比度,并在MDAMB231接种荷瘤鼠3小时内光声信号比对照组U87MG荷瘤鼠强10倍以上,从而证明其是一种良好的叶酸受体靶向探针,有望在乳腺癌早期诊断分子影像与影像辅助治疗中得到广泛应用。
The early detection and effective diagnosis of breast cancers are challenging to our life.Photoacoustic(PA)imaging combines the optical high sensitivity and acoustic deep penetration with satisfactory image resolution.Herein,hybrid polymeric methylene blue-embedded nanoprobes(PBCA-MB@CS-FA) for folate receptor-targeted PA imaging have been successfully fabricated.Phantom imaging and in vitro cell experiments confirmed their apparent contrast enhancement and strong binding affinity.The administration into MDA-MB231 tumor-xenografted nude mice showed 10-fold higher intensity of PA signals compared to that of non-targeted control.These findings implied that they are good candidates for non-invasive molecular diagnosis and potential image-guided therapy at early stage of breast cancers.
The early detection and effective diagnosis of breast cancers are challenging to our life.Photoacoustic(PA)imaging combines the optical high sensitivity and acoustic deep penetration with satisfactory image resolution.Herein,hybrid polymeric methylene blue-embedded nanoprobes(PBCA-MB@CS-FA) for folate receptor-targeted PA imaging have been successfully fabricated.Phantom imaging and in vitro cell experiments confirmed their apparent contrast enhancement and strong binding affinity.The administration into MDA-MB231 tumor-xenografted nude mice showed 10-fold higher intensity of PA signals compared to that of non-targeted control.These findings implied that they are good candidates for non-invasive molecular diagnosis and potential image-guided therapy at early stage of breast cancers.
引文
[1]Yao J.;Wang L.V.Constrast Media Mol.Imaging 2011,6:332.
[2]Li K.;Liu B.Chem.Soc.Rev.2014,43:6570.
[2]Li K.;Liu B.Chem.Soc.Rev.2014,43:6570.