牛源金黄色葡萄球菌随机扩增多态性分型
详细信息 查看官网全文
- 英文论文题名：Research on genetic polymorphism of Staphylococcus aureus isolated from bovine
- 论文作者：武中庸 ; 倪春霞 ; 赵吴静 ; 蒲万霞
- 英文论文作者：Wu zhong-yong ; Ni chun-xia ; Zhao wu-jing ; Pu wan-xia ; Key Laboratory of New Animal Drug of Gansu Province ; Key Laboratory of New Animal Drug ; Lanzhou Institute of Animal Sciences and Veterinary Pharmaceutics ; Chinese Academy of Agricultural Sciences
- 年：2016
- 作者机构：中国农业科学院兰州畜牧与兽药研究所中国农业科学院新兽药工程重点开放实验室甘肃省新兽药工程重点实验室;
- 论文关键词：奶牛乳房炎 ; 金黄色葡萄球菌 ; RAPD ; 基因型
- 英文论文关键词：bovine mastitis ; Staphylococcus aureus ; RAPD ; genotype
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S852.611
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：68k
- 原文格式：O
- 会议级别：全国
摘要
利用引物AP7建立随机引物多态性扩增体系对176株引起我国不同地区奶牛乳房炎的金黄色葡萄球菌分离株进行基因分型研究。结果表明,176株金黄色葡萄球菌均得到清晰的RAPD指纹图谱,扩增产物为2~9条带,产物大小为240~4500bp。菌株共分为12个基因型,其中Ⅰ型35株(20%);Ⅱ型和Ⅷ型各7株(各4%);Ⅲ型和Ⅺ型各6株(各3.4%);Ⅳ型17株(9.8%);Ⅴ型4株(2.2%);Ⅵ型43株(25%);Ⅶ型各12株(6.9%);Ⅸ型和Ⅻ型各9株(5.2%);Ⅹ型3株(1.7%)。Ⅰ型和Ⅵ型菌株占总菌株数45%以上,为主要流行的优势菌群。各地区基因型菌株分布比例有明显差异,可能与奶牛养殖业水平和环境差异有关。
Randomly amplified polymorphic DNA with three oligonucleotide primers were used for research on genetic polymorphism of 176 Staphylococcus aureus(S.aureus) strains isolated from dairy cattle in different regions of china.The result shows that all of the 176 S.aureus strains produced fingerprint distinctly and amplified out 2~9 electrophoresis strips which reveal DNA markers with sizes ranging from 240~4500bp.The S.aureus strains were separated into 12 genotype by fingerprint with oligonucleotide primers AP7 and 35 strains were type Ⅰ(20%),7 strains were each typeⅡ and Ⅷ(4%),6 strains were each type Ⅲ and Ⅺ(3.4%),17 strains were type Ⅳ(9.8%),5 strains were type Ⅴ(2.2%),43 strains were type Ⅵ(25%),12 strains were type Ⅶ(6.9%),9 strains were each type Ⅸ and Ⅻ(5,.2%),3 strains were type Ⅹ(1.7%).Difference of dairy farming level,geographical environment and using of antibiotic may be the reason of distinct discrepancy on proportion of different genotype strains in those areas.
Randomly amplified polymorphic DNA with three oligonucleotide primers were used for research on genetic polymorphism of 176 Staphylococcus aureus(S.aureus) strains isolated from dairy cattle in different regions of china.The result shows that all of the 176 S.aureus strains produced fingerprint distinctly and amplified out 2~9 electrophoresis strips which reveal DNA markers with sizes ranging from 240~4500bp.The S.aureus strains were separated into 12 genotype by fingerprint with oligonucleotide primers AP7 and 35 strains were type Ⅰ(20%),7 strains were each typeⅡ and Ⅷ(4%),6 strains were each type Ⅲ and Ⅺ(3.4%),17 strains were type Ⅳ(9.8%),5 strains were type Ⅴ(2.2%),43 strains were type Ⅵ(25%),12 strains were type Ⅶ(6.9%),9 strains were each type Ⅸ and Ⅻ(5,.2%),3 strains were type Ⅹ(1.7%).Difference of dairy farming level,geographical environment and using of antibiotic may be the reason of distinct discrepancy on proportion of different genotype strains in those areas.
引文
[1]Denis M,Wedlock D N,Lacy-Hulbert S J,et al.Vaccines against bovine mastitis in the New Zealand context:what is the best way for ward[J].N Z Vet J,2009,57(3):132-140
[2]史冬艳,郝永清,张爱荣,等.奶牛金黄色葡萄球菌性乳房炎研究进展[J].动物医学进展,2010,31(7):82-86
[3]Supre K,Lommelen K,Meulemeester LD.Antimicrobial susceptibility and distribution of inhibition zone diameters of bovine mastitis pathogens in Flanders,Belgium[J].Vet Microbiol,2014,171(3-4):374-381.
[4]高潮,刘国庆,赵莉莉,等.上海地区奶牛乳房炎金黄色葡萄球菌基因分型与耐药性研究[J].上海交通大学学报,2014,32(1):53-58
[5]Williams JG,Kubelik AR,Livak KJ,et al.DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J].Nucleic Acids Res,1990,18(22):6531-6535
[2]史冬艳,郝永清,张爱荣,等.奶牛金黄色葡萄球菌性乳房炎研究进展[J].动物医学进展,2010,31(7):82-86
[3]Supre K,Lommelen K,Meulemeester LD.Antimicrobial susceptibility and distribution of inhibition zone diameters of bovine mastitis pathogens in Flanders,Belgium[J].Vet Microbiol,2014,171(3-4):374-381.
[4]高潮,刘国庆,赵莉莉,等.上海地区奶牛乳房炎金黄色葡萄球菌基因分型与耐药性研究[J].上海交通大学学报,2014,32(1):53-58
[5]Williams JG,Kubelik AR,Livak KJ,et al.DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J].Nucleic Acids Res,1990,18(22):6531-6535