Illumina MiSeq测序平台测定蒙古羊瘤胃液相和固相菌群多样性
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- 英文论文题名：Bacteria Community Diversity of Liquid and Solid Phases of Ruminal Contents of Mongolian Sheep Analyzed by Illumina MiSeq Platform
- 论文作者：曾燕 ; 简平 ; 倪学勤 ; 周毅 ; 祝辉 ; 曾东
- 英文论文作者：ZENG Yan ; JIAN Ping ; NI Xueqin ; ZHOU Yi ; ZHU Hui ; ZENG Dong ; Institute of Animal Microecology ; College of Veterinary Medicine ; Sichuan Agricultural University ; Key Laboratory of Animal Disease and Human Health of Sichuan Province
- 年：2016
- 作者机构：四川农业大学动物微生态研究中心;动物疫病与人类健康四川省重点实验室;
- 论文关键词：Illumina MiSeq ; 液相 ; 固相 ; 细菌 ; 多样性
- 英文论文关键词：Illumina MiSeq ; liquid phase ; solid phase ; bacteria ; diversity
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S826.82;S852.6
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：92k
- 原文格式：O
- 会议级别：全国
摘要
本研究旨在比较蒙古羊瘤胃液相和固相细菌菌群多样性。应用Illumina Miseq测序平台的16S rRNA基因扩增子技术对采集自5只蒙古羊的瘤胃液相和固相样品进行高通量测序,采用UPARSE-操作分类单元(OTU)法和非加权组平均(UPGMA)法分析试验结果。结果表明,共获得91 971条序列和15 321个OTU,平均每个样品有13 138条序列和2 189个OTU。液相与固相样品相比:门水平分类上存在显著性差异的优势菌群为拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)(P<0.05),拟杆菌门在液相和固相样品中的比例分别为67%和45%;属水平分类上存在显著性差异的优势菌群为普氏菌属(Prevotella)和1种未鉴定(unknown)的属(P<0.05),液相样品优势菌群为普氏菌属,所占比例为53%,而固相样品优势菌群来自1种未鉴定(unknown)的属,所占比例为32%;种水平分类上存在显著性差异的是栖瘤胃普雷沃菌(Prevotella ruminicola)(P<0.05),在液相和固相样品中所占的比例分别为41%和11%。与液相样品相比,固相样品的alpha多样性较好;主成分分析(PCA)和主坐标分析(PCoA)显示来自同一动物个体的瘤胃液相和固相样品聚类在一起。结果提示,蒙古羊瘤胃液相和固相样品均具有丰富的细菌菌群;与液相样品相比,固相样品细菌的多样性更好。
This experiment was conducted to compare bacteria community diversity of liquid and solid phases of ruminal contents of Mongolian sheep.The samples of liquid and solid phases of ruminal contents from 5 Mongolian sheep were investigated using 16 S rRNA amplicon high-throughput sequencing technique by Illumina MiSeq platform,and the results were analyzed by UPARSE-operational taxonomic units(OTU) and unweighted pair group method with arithmetic mean(UPGMA).The results showed that a total of 91 971 sequences and 1 871 OTUs across all samples were observed,and the average number of sequence and OTU was 13 138 and 267 per sample,respectively.Compared between the samples of liquid and solid phases:at phyla level,dominant bacteria with significant differences were Bacteroidetes and Firmicutes,and the percentage of Bacteroidetes of the samples of liquid and solid phases was 67%and 45%,respectively;at genus level,dominant bacteria with significant differences were Prevotella and an unknown genus,dominant bacterium of liquid phase was Prevotella whose percentage reached 53%,and that of solid phase was the unknown genus whose percentage reached 32%;at species level,dominant bacteria with significant differences were Prevotella ruminicola,and the percentage of liquid and solid phases reached 41%and 11%,respectively.The alpha diversity of solid phase was higher compared with that of liquid phase,and the samples from the same animal clustered together by principal component analysis(PCA) and principal coordinate analysis(PCoA).In conclusion,both the contents of liquid and solid phases have high diversity of bacteria,and the diversity of solid phase is higher compared with that of liquid phase.
This experiment was conducted to compare bacteria community diversity of liquid and solid phases of ruminal contents of Mongolian sheep.The samples of liquid and solid phases of ruminal contents from 5 Mongolian sheep were investigated using 16 S rRNA amplicon high-throughput sequencing technique by Illumina MiSeq platform,and the results were analyzed by UPARSE-operational taxonomic units(OTU) and unweighted pair group method with arithmetic mean(UPGMA).The results showed that a total of 91 971 sequences and 1 871 OTUs across all samples were observed,and the average number of sequence and OTU was 13 138 and 267 per sample,respectively.Compared between the samples of liquid and solid phases:at phyla level,dominant bacteria with significant differences were Bacteroidetes and Firmicutes,and the percentage of Bacteroidetes of the samples of liquid and solid phases was 67%and 45%,respectively;at genus level,dominant bacteria with significant differences were Prevotella and an unknown genus,dominant bacterium of liquid phase was Prevotella whose percentage reached 53%,and that of solid phase was the unknown genus whose percentage reached 32%;at species level,dominant bacteria with significant differences were Prevotella ruminicola,and the percentage of liquid and solid phases reached 41%and 11%,respectively.The alpha diversity of solid phase was higher compared with that of liquid phase,and the samples from the same animal clustered together by principal component analysis(PCA) and principal coordinate analysis(PCoA).In conclusion,both the contents of liquid and solid phases have high diversity of bacteria,and the diversity of solid phase is higher compared with that of liquid phase.
引文
[1]KEBREAB E,DIJKSTRA J,BANNINK A,et al.Recent advances in modeling nutrient utilization in ruminants[J].Journal of Animal Science,2009,87(14):E111-E122.