高效液相色谱法同时测定消毒剂速可净中枸橼酸和苹果酸的含量
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- 英文论文题名：Simultaneous determination of the content of citric acid and malic acid in Snucop by HPLC
- 论文作者：梁劲康 ; 方炳虎 ; 黎乃添 ; 吴志玲 ; 张桂君
- 英文论文作者：LIANG Jing-kang ; FANG Bing-hu ; LI Nai-tian ; WU Zhi-ling ; ZHANG Gui-jun ; Guangdong WenshiDahuanong Biotechnological Co. ; Ltd.
- 年：2016
- 作者机构：广东温氏大华农生物科技有限公司;
- 论文关键词：枸橼酸 ; 苹果酸 ; 速可净 ; 高效液相色谱法 ; 含量测定
- 英文论文关键词：Citric acid ; Malic acid ; Snucop ; HPLC ; Content determination
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S859.79;O657.72
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：6
- 文件大小：308k
- 原文格式：O
- 会议级别：全国
摘要
建立同时测定兽用消毒剂速可净中枸橼酸和苹果酸的高效液相色谱方法。采用Agilent Eclipse XDB-C18(250mm×4.6 mm,5μm)色谱柱,以乙腈-1.0%磷酸溶液(2.5:97.5,v/v)为流动相进行洗脱,流速为1.0 m1-/min,检测波长为210 nm,柱温为35℃,进样量为20νL。结果表明,枸橼酸和苹果酸色谱峰分离度良好。枸橼酸和苹果酸分别在100~1000μg/mL和50~500μg/mL范围内线性关系良好。精密度、稳定性和重现性等考察结果的相对标准偏差(RSD)均小于1%;而平均回收率均在98%~101%之间,RSD均小于0.5%,均符合方法学要求。6份速可净样品中枸橼酸和苹果酸的含量也均符合要求。建立的高效液相色谱法能够同时定量测定枸橼酸和苹果酸的含量,可用于速可净的质量控制。
To establish a HPLC method for simultaneous determination of citric acid and malic acid in Snucop.The assay was conducted on an Agilent Eclipse XDB-C18 column(250mm×4.6 mm,5 μm) with acetonitrile-1.0%H_3PO_4 solution(2.5:97.5.v/v) as mobile phase.The flow rate was 1.0 mL/min,the detection wavelength was 210 nm,the column temperature was 35℃ and the injection volume was 20 μL.The results showed good resolution for the chromatographic peaks of citric acid and malic acid.The correlation coefficient between concentration and chromatographic peak area was over 0.999 in the range of 100~1000 μg/mL for citric acid and 50~500 μg/mL for malic acid,indicating the good linearity of the calibration curves.In the precision,stability and repeated experiments,the relative standard deviations of citric acid and malic acid were less than 1%,showing a satisfactory precision and stability of this method.In the spiked experiment of three levels,the average recovery of citric acid and malic acid was about 98%~101%and all the relative standard deviations were less than 0.5%,demonstrating that this method was considerably accurate.This HPLC method can be used for the quantitative determination of citric acid and malic acid simultaneously and the quality control in Snucop.
To establish a HPLC method for simultaneous determination of citric acid and malic acid in Snucop.The assay was conducted on an Agilent Eclipse XDB-C18 column(250mm×4.6 mm,5 μm) with acetonitrile-1.0%H_3PO_4 solution(2.5:97.5.v/v) as mobile phase.The flow rate was 1.0 mL/min,the detection wavelength was 210 nm,the column temperature was 35℃ and the injection volume was 20 μL.The results showed good resolution for the chromatographic peaks of citric acid and malic acid.The correlation coefficient between concentration and chromatographic peak area was over 0.999 in the range of 100~1000 μg/mL for citric acid and 50~500 μg/mL for malic acid,indicating the good linearity of the calibration curves.In the precision,stability and repeated experiments,the relative standard deviations of citric acid and malic acid were less than 1%,showing a satisfactory precision and stability of this method.In the spiked experiment of three levels,the average recovery of citric acid and malic acid was about 98%~101%and all the relative standard deviations were less than 0.5%,demonstrating that this method was considerably accurate.This HPLC method can be used for the quantitative determination of citric acid and malic acid simultaneously and the quality control in Snucop.
引文
[1]魏全意,董强.速可净(SNIJCOP)与猪场消毒[J].养殖与饲料,2007,10:35-37.
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[4]唐慧慧,蔡清宇,毛翼.HPLC法测定净乌梅及乌梅炭中柠檬酸和苹果酸的含量[J].中药材,2007,30(1):52-54.
[5]吴玉萍,宋春满,雷丽萍等.梯度淋洗离子色谱法测定烟草中的苹果酸、柠檬酸和阴离子[J].分析试验室,2006,25(7):31-34.
[6]张韵,傅颖,吴霞红.五味子药材中苹果酸、柠檬酸的测定方法研究[J].中成药,2012,34(4):767-769.
[7]刘森泉,阮红,徐玲芬等.反相高效液相色谱法测定发酵液中β-聚苹果酸含量[J].食品与发酵工业,2010,36(6):142-145.
[2]曾昭智,张锦红.三种消毒剂对屏障环境设施消毒效果观察[J].黑龙江畜牧兽医,2013,14:108-109.
[3]吕兆林,林西,邓文红等.HPLC法测定苹果酒中苹果酸及乳酸含量[J].精细与专用化学品,2012,20(1):9-13.
[4]唐慧慧,蔡清宇,毛翼.HPLC法测定净乌梅及乌梅炭中柠檬酸和苹果酸的含量[J].中药材,2007,30(1):52-54.
[5]吴玉萍,宋春满,雷丽萍等.梯度淋洗离子色谱法测定烟草中的苹果酸、柠檬酸和阴离子[J].分析试验室,2006,25(7):31-34.
[6]张韵,傅颖,吴霞红.五味子药材中苹果酸、柠檬酸的测定方法研究[J].中成药,2012,34(4):767-769.
[7]刘森泉,阮红,徐玲芬等.反相高效液相色谱法测定发酵液中β-聚苹果酸含量[J].食品与发酵工业,2010,36(6):142-145.