基于iTRAQ技术的ISKNV感染CPB细胞蛋白组分析
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- 英文论文题名：Proteomic Analysis of Mandarin Fish Brain Cells Infected with Infectious Spleen and Kidney Necrosis Virus by iTRAQ Approach
- 论文作者：吴仕伟 ; 付小哲 ; 林强 ; 刘礼辉 ; 梁红茹 ; 黄志斌 ; 李宁求
- 英文论文作者：WU Shiwei ; FU Xiaozhe ; LIN Qiang ; LIU Lihui ; LIANG Hongru ; HUANG Zhibin ; LI Ningqiu ; Pearl River Fisheries Research Institute ; Chinese Academy of Fishery Sciences ; Key Laboratory of Fishery Drug Development ; Ministry of Agriculture ; Key Laboratory of Aquatic Animal Immune Technology
- 年：2016
- 作者机构：中国水产科学研究院珠江水产研究所农业部渔用药物创制重点实验室广东省水产动物免疫技术重点实验室;上海海洋大学水产与生命学院;淡水水产健康养殖湖北省协同创新中心;
- 论文关键词：ISKNV ; 鳜鱼脑细胞 ; 蛋白组
- 英文论文关键词：ISKNV ; Mandarin fish brain cell ; Proteomics
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S943
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：552k
- 原文格式：D
- 会议级别：全国
摘要
由传染性脾肾坏死病毒(ISKNV)感染导致的病毒病给我国鳜鱼养殖带来严重危害。为从全局角度探究ISKNV与宿主细胞的相互作用,本研究采用iTRAQ技术分析了ISKNV感染鳜鱼脑细胞(CPB)后24h和72h蛋白组,共获得6363个唯一肽段,其中2731个蛋白与已知蛋白同源。差异蛋白分析表明:24h感染组相对对照组共有232个差异蛋白,其中141个蛋白上调,91个蛋白下调;72h感染组相对对照组共有199个差异蛋白,其中111个蛋白上调,88个蛋白下调。蛋白功能富集分析显示差异蛋白与生物调节、单有机体过程、代谢过程、细胞转化、催化活性、绑定分子功能、大分子配合物、细胞和细胞器有关。通过免疫印迹验证了G6PDH、β-tubulin和RPL11的蛋白在不同组之间的差异与i TRAQ结果一致,表明i TRAQ得到的差异蛋白组结果可靠。本研究为进一步研究ISKNV感染致病机制和寻找潜在的药物靶标奠定基础。
Infectious spleen and kidney necrosis virus(ISKNV), the causative agent of Mandarin fish(Siniperca chuatsi) infectious spleen and kidney necrosis,has drastic effects on Mandarin fish fisheries in our country. Aiming to detectthe interactions between ISKNV andhost from a whole perspective, i TRAQ technique was used for analyzing the proteomic of Mandarin fish brain cell(CPBs) at 24 h and 72 h after ISKNVinfection. A total of 6363 unique peptides corresponding to2731 proteins were identified, and there respectivelywere 232 which including 141 up-regulated proteins and 91 down-regulated proteinsand 199 which including 111 up-regulated proteins and 88down-regulated proteins altered proteins at 24 h and 72 h comparing with mock-infected cells.Gene ontology analysis demonstrated that a number of proteins were related to biological regulation,single-organism process, metabolic process, cellular process, catalytic activity, binding,macromolecular complex, celland organelle. Moreover,the western blot analysis demonstrated the changes ofsome proteins level between different groups including G6PDH、β-tubulin and RPL11,which corresponded to the resultsof i TRAQ and verified the reliability of the proteomics data.
Infectious spleen and kidney necrosis virus(ISKNV), the causative agent of Mandarin fish(Siniperca chuatsi) infectious spleen and kidney necrosis,has drastic effects on Mandarin fish fisheries in our country. Aiming to detectthe interactions between ISKNV andhost from a whole perspective, i TRAQ technique was used for analyzing the proteomic of Mandarin fish brain cell(CPBs) at 24 h and 72 h after ISKNVinfection. A total of 6363 unique peptides corresponding to2731 proteins were identified, and there respectivelywere 232 which including 141 up-regulated proteins and 91 down-regulated proteinsand 199 which including 111 up-regulated proteins and 88down-regulated proteins altered proteins at 24 h and 72 h comparing with mock-infected cells.Gene ontology analysis demonstrated that a number of proteins were related to biological regulation,single-organism process, metabolic process, cellular process, catalytic activity, binding,macromolecular complex, celland organelle. Moreover,the western blot analysis demonstrated the changes ofsome proteins level between different groups including G6PDH、β-tubulin and RPL11,which corresponded to the resultsof i TRAQ and verified the reliability of the proteomics data.
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