低强度超声波对热带假丝酵母的促增殖效应及其机制研究
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- 英文论文题名：The influence of low intensity ultrasonic irradiation treatment on the proliferation of Candida tropicalis and its mechanisms
- 论文作者：何荣海 ; 熊锋 ; 侯福荣 ; 邢欢
- 英文论文作者：He Ronghai ; Xiong feng ; Hou Furong ; Xing Huan ; School of Food and Biological Engineering ; Jiangsu University
- 年：2016
- 作者机构：江苏大学食品与生物工程学院;
- 论文关键词：低强度超声 ; 热带假丝酵母 ; 增殖效应 ; 转录组测序
- 英文论文关键词：low intensity ultrasound ; candida tropicalis ; proliferation ; RNA-Seq
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：Q93-33
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：104k
- 原文格式：O
- 会议级别：全国
摘要
本研究以热带假丝酵母菌(Candida tropicalis)为对象,研究了超声场引起的热带假丝酵母细胞增殖、形态、细胞膜通透性等生物学变化,并利用转录组测序技术阐释低强度超声调控热带假丝酵母细胞周期的分子机制。主要的研究结果如下:热带假丝酵母菌的生长时期、超声频率、超声功率以及超声时间等都会对超声场的增殖效果产生影响,其中对培养到对数中期的热带假丝酵母菌液以频率28 kHz、功率密度120 W/L超声处理1h,菌体生物量增加量比对照组提高148.5%。采用Fluo-4/AM荧光探针法研究了发现,低强度超声处理后热带假丝酵母胞内Ca~(2+)荧光强度下降,超声2h后下降至最大值为25.8%。转录组测序技术筛选差异表达基因系列分析结果显示,超声促增殖效应的关键基因是CTRG-01717(phosphatidylinositol 3-kinase TOR2),其基因表达上调14.4倍。在GO富集分析和KEGG富集分析结果表明,TOR2属于GO分子功能top20中Protein serine/threonine kinase activity(蛋白质的丝氨酸/苏氨酸激酶活性)和KEGG pathway top20中PI3K-Akt signaling pathrway(磷脂酰激醇-3-羟激酶信号通路),AMPK signalling pathway(蛋白激酶信号通路)的基因。
In this study,Candida tropicalis was used as the object to be stimulated by ultrasonic field and the biological changes of proliferation,morphology,cell membrane were studied.Also,the molecular mechanism of the transcriptome sequencing was investigated to explain how the low intensity ultrasonic regulate the cell cycle of Candida tropicalis.The main results are as follows;It showed that the proliferation was affected by growth phase,ultrasonic power intensity,ultrasonic frequency and processing time,etc.The optimum ultrasonic treatment conditions for the maximum biomass addition of Candida tropicaliswere determined as follows;28 kHz ultrasound irradiation with power intensity 120 W/L,Candida tropicalis culture at mid logarithmic phase,and treating for 1 hour.Under these conditions,the Candida tropicalis biomass addition reaches to148.5%.Fluo-4/AM fluorescent probe method was used to explore the transmembrane behavior of intracellular Ca~(2+) under ultrasonic treatment.After 2h time of low intensity ultrasound treatment,the inner cellular Ca~(2+) fluorescence intensity decreased 25.8%.Transcriptome sequencing results showed that CTRG-01717(phosphatidylinositol 3-kinase TOR2) was the key genes of proliferation combined with the differential expression analysis,the expression of TOR2 gene increased 14.4times,and this gene belongs to Protein serine/threonine kinase activity,PI3K-Akt signaling pathway and AMPK signaling pathway.
In this study,Candida tropicalis was used as the object to be stimulated by ultrasonic field and the biological changes of proliferation,morphology,cell membrane were studied.Also,the molecular mechanism of the transcriptome sequencing was investigated to explain how the low intensity ultrasonic regulate the cell cycle of Candida tropicalis.The main results are as follows;It showed that the proliferation was affected by growth phase,ultrasonic power intensity,ultrasonic frequency and processing time,etc.The optimum ultrasonic treatment conditions for the maximum biomass addition of Candida tropicaliswere determined as follows;28 kHz ultrasound irradiation with power intensity 120 W/L,Candida tropicalis culture at mid logarithmic phase,and treating for 1 hour.Under these conditions,the Candida tropicalis biomass addition reaches to148.5%.Fluo-4/AM fluorescent probe method was used to explore the transmembrane behavior of intracellular Ca~(2+) under ultrasonic treatment.After 2h time of low intensity ultrasound treatment,the inner cellular Ca~(2+) fluorescence intensity decreased 25.8%.Transcriptome sequencing results showed that CTRG-01717(phosphatidylinositol 3-kinase TOR2) was the key genes of proliferation combined with the differential expression analysis,the expression of TOR2 gene increased 14.4times,and this gene belongs to Protein serine/threonine kinase activity,PI3K-Akt signaling pathway and AMPK signaling pathway.
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