烤烟品种K326及其耐低温抗早花变异系基因表达谱分析
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- 英文论文题名：Gene Expression Profile Analysis of Flue-Cured Tobacco K326 and The Premature Flowering Resistance Varieties
- 论文作者：杨静 ; 陈杰 ; 陈建军 ; 邓世媛 ; 邱妙文 ; 赵伟才 ; 王维
- 英文论文作者：YANG Jing ; CHEN Jie ; CHEN Jianjun ; DENG Shiyuan ; QIU Miaowen ; ZHAO Weicai ; WANG Wei ; Qian Dongnan Zhenyuan Tobacco Company ; College of Agriculture ; South China Agricultural University ; Qian Dongnan Tobacco Company ; Nanxiong Institute of Tobacco Science ; Guangdong Provincial Tobacco Company
- 年：2016
- 作者机构：黔东南州烟草公司镇远县分公司;华南农业大学农学院;黔东南州烟草公司;广东省烟草南雄科学研究所;
- 论文关键词：烤烟 ; 早花 ; 抗早花 ; 基因芯片 ; 表达谱
- 英文论文关键词：flue-cured tobacco ; premature flowering ; premature flowering resistance ; gene chip ; expression profile
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：S572
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：7
- 文件大小：1376k
- 原文格式：D
摘要
为了解烤烟早花与抗早花的形成及调控机制,本研究利用安捷伦烟草全基因组芯片分析了烤烟品种K326及其抗早花品系华烟06茎尖端花芽分化过程中基因的表达。结果表明,K326与华烟06距离各自花芽分化相同时间的茎尖端未花芽分化时的差异探针数最多,有5295个;K326分化当天各品种(品系)之间相比较差异探针数量最少,有2116个。与分子功能相关的差异基因主要与催化性能、转运性能、结合性能和转录调节性能有关;与细胞组成相关的差异基因主要是与细胞、细胞组分、细胞器相关的基因;与生物学过程相关的差异基因种类最多最复杂,涉及到植株抗逆性、生长、发育等方面,这对进一步研究与从中搜寻与烟草开花相关的基因奠定了基础。
To research the regulation and formation mechanism of premature flowering and premature flowering resistance of flue-cured tobacco, in this study, we used the Agilent Tobacco Oligo Microarray to deduce the expression profiling of flue-cured tobacco K326 and its premature flowering resistance variety Huayan06 under the flower bud differentiation process. The result showed that the same days from their flower bud differentiation the number of differential probes were the maximum amount of the comparative item between K326 and Huayan06. The number of the probe was 5295. On the day of K326 flower bud differentiation, the number of differential probe was the minimum amount. The number of the probe was 2116. The molecular function mainly included catalytic activity, transporter activity, binding and transcription regulator activity. The cellular component mainly included cell, cell part and organelle. The biological process was the most complex, involved plant resistance, growth, development, etc. It would establish a basis for the further study and search for genes associated with tobacco flowering.
To research the regulation and formation mechanism of premature flowering and premature flowering resistance of flue-cured tobacco, in this study, we used the Agilent Tobacco Oligo Microarray to deduce the expression profiling of flue-cured tobacco K326 and its premature flowering resistance variety Huayan06 under the flower bud differentiation process. The result showed that the same days from their flower bud differentiation the number of differential probes were the maximum amount of the comparative item between K326 and Huayan06. The number of the probe was 5295. On the day of K326 flower bud differentiation, the number of differential probe was the minimum amount. The number of the probe was 2116. The molecular function mainly included catalytic activity, transporter activity, binding and transcription regulator activity. The cellular component mainly included cell, cell part and organelle. The biological process was the most complex, involved plant resistance, growth, development, etc. It would establish a basis for the further study and search for genes associated with tobacco flowering.
引文
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[2]张国,朱列书,王奎武,等.烟草早花研究进展[J].作物研究,2005(5):383-385.
[3]种康,雍伟东,谭克辉.高等植物春化作用研究进展[J].植物学通报,1999,16(5):481-487.
[4]Yanfsky M F,Ma H,Bowman J H,et al.The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors.Nature,1990,346:35-39.
[5]Horvath,D P.Common mechanisms regulate flowering and dormancy[J].Plant Science,2009,177(6):523-531.
[6]Matsuda N,Ikeda K,Kurosaka M,et al.Early flowering phenotype in transgenic pears(Pyrus communis L.)expressing the Ci FT gene[J].Japan.Soc.Hort.Sci,2009,78(4):410-416.
[7]许志茹,李玉花.基因芯片技术在植物研究中的应用[J].生物技术,2004,14(6):70-72.
[8]朱素琴,季本华,陈名蔚.高盐低温胁迫下水稻转录因子基因表达谱分析[J].科技通报,2010,26(6):844-852.
[9]宋雯雯,李文滨,韩雪.干旱胁迫下大豆幼苗根系基因的表达谱分析[J].中国农业科学学术期刊,2010,43(22):4579-4586.
[10]许州达,景瑞莲,甘强,等.用水稻基因芯片筛选小麦耐旱相关基因[J].农业生物技术学报,2007,15(5):821-827.
[11]曾黎琼,张伟,段玉云,等.利用基因芯片分析非洲菊花序发育相关基因的表达[J].西南农业学报,2009,22(3):759-763.
[12]Lee D,Polisensky D H,Braam J.Genome-wide identification of touch and darkness-regulated arabidopsis genes:a focus on calmodulin-like and XTH genes[J].New Phytologist,2005,165(2):429-444.
[13]刘明,王米渠,丁维俊,等.表达谱芯片数据的基因功能富集分析[J].生物医学工程学杂志,2010,27(5):1166-1168,1196.