可催化黄酮酰化反应的施氏假单胞菌细胞联接酶的定位及活性研究
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- 英文论文题名：Catalytic activity and localization of cell-bound lipases in Pseudomonas stutzeri cells for flavonoids acylation
- 论文作者：辛璇 ; 李晓凤
- 英文论文作者：Xuan Xin ; Xiaofeng Li ; South China University of Technology ; College of Food Science and Engineering
- 年：2016
- 作者机构：华南理工大学食品科学与工程学院;
- 论文关键词：施氏假单胞菌 ; 脂肪酶 ; 酶定位 ; 秦皮甲素 ; 酰化反应
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十三分会：绿色化学
- 语种：中文
- 分类号：O629.8;O643.36
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十三分会 ; 绿色化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：150k
- 原文格式：D
- 会议级别：全国
摘要
黄酮类化合物是一类天然多酚类化合物,具有抗氧化、抗癌和抗病毒、抗菌等多种生理功能,对其进行酰化修饰可以提高其理化性质和增强其生理活性。本研究筛选获得了可高效催化黄酮类化合物酰化反应的施氏假单胞菌催化剂,利用超声破碎-溶剂提取等方式分别制备了该细胞的不同细胞组分粗酶蛋白,以秦皮甲素酰化反应为模型,对具有该酰化活性的脂肪酶进行了细胞定位。通过对不同时段培养的微生物细胞的产酶位点进行动态监测,结果发现,施氏假单胞菌的细胞质、细胞膜和细胞壁均含有可催化秦皮甲素酰化反应的脂肪酶。全细胞和各组分脂肪酶的催化活力在初始培养阶段均呈上升趋势,培养中期趋于稳定,但在培养后期,胞连接酶的催化活力呈现下降趋势,表明培养时间会影响全细胞催化剂的催化效果。本研究确定了假单胞菌全细胞联接酶在细胞中的分布定位,并探讨了培养过程中各组分酶蛋白的酰化活性变化规律,对高效全细胞生物催化剂的制备及应用具有重要的参考意义。
Flavonoids are a kind of natural polyphenolic compounds with serious of physiological functions including anti-oxidant, anti-tumor, and antiviral. Acylation of flavonoids have been proved to improve its solubility in oils, thereby enhancing their bioactivity. This research has been screened P.stutzeri catalyzing efficiently the acylation of flavonoids, using the way of ultrasonication-solvent extraction to obtain the crude protein of different cellular components, in which the acylation of esculin was used as model to locate the lipase with acylated activity in cells. Through dynamic monitoring the site of lipase production in cells cultured in different time, the research determined localization of pseudomonas lipase in cells, and discussed the acylated activity change rule of each of components lipase in the process of cultivation. It has important reference meaning for production and application of efficient whole-cell biocatalyst.
Flavonoids are a kind of natural polyphenolic compounds with serious of physiological functions including anti-oxidant, anti-tumor, and antiviral. Acylation of flavonoids have been proved to improve its solubility in oils, thereby enhancing their bioactivity. This research has been screened P.stutzeri catalyzing efficiently the acylation of flavonoids, using the way of ultrasonication-solvent extraction to obtain the crude protein of different cellular components, in which the acylation of esculin was used as model to locate the lipase with acylated activity in cells. Through dynamic monitoring the site of lipase production in cells cultured in different time, the research determined localization of pseudomonas lipase in cells, and discussed the acylated activity change rule of each of components lipase in the process of cultivation. It has important reference meaning for production and application of efficient whole-cell biocatalyst.
引文
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