三种类型初级代谢物对降低副溶血性弧菌耐药性作用效果的比较研究

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• 英文论文题名：Comparative analysis on the effect of three types of primary metabolites on reducing the resistance of antibiotic-resistant vibrio parahemolyticus
• 论文作者：王冰 ; 何新宇 ; 周洋洋 ; 张炜佳 ; 潘迎捷 ; 赵勇
• 英文论文作者：Wang Bing ; He Xingyue ; Zhou Yangyang ; Zhang Weijia ; Pan Yingjie ; Zhao Yong ; College of Food Science and Technology ; Shanghai Ocean University ; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation ; Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation(Shanghai) ; Ministry of Agriculture
• 年：2016
• 作者机构：上海海洋大学食品学院;上海水产品加工及贮藏工程技术研究中心;农业部水产品贮藏保鲜质量安全风险评估实验室;
• 论文关键词：副溶血性弧菌 ; 外源代谢物 ; 多重耐药 ; RT-PCR
• 英文论文关键词：vibrio parahemolyticus ; exogenous metabolite ; multi-drug resistance ; RT-PCR
• 会议召开时间：2016-11-09
• 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
• 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
• 语种：中文
• 分类号：R378
• 学会代码：ZGSP
• 会议名称：中国食品科学技术学会第十三届年会
• 会议地点：中国北京
• 主办单位：中国食品科学技术学会
• 学会名称：中国食品科学技术学会
• 页数：2
• 文件大小：108k
• 原文格式：O
• 会议级别：全国

摘要

【目的】通过调节细胞代谢状态可重构耐药副溶血性弧菌对氨基糖苷类抗生素杀菌作用的敏感性。从而创建了一种有效杀死耐药副溶血性弧菌的新途径。【方法】通过表型实验比较了在亚致死浓度抗生素作用下,不同种类代谢物对耐药细菌存活率的作用影响。并通过RT-PCR进一步分析了氧化磷酸化偶联质子通道中相关重要基因sucC,gltA,nuoF,nuol,pfkA表达量随时间的变化。【结果】在分别添加10mM甘油,丙氨酸,葡萄糖代谢物培养6h后,细菌对低浓度抗生素的敏感性提高了64倍,101倍,1886倍。对照组经氧化磷酸化解偶联剂CCCP处理后,外源代谢物的添加失去作用。【结论】通过调节细菌细胞代谢水平可显著提高耐药弧菌对氨基糖苷类抗生素的敏感性。作用机制可能主要依赖于外源代谢物通过底物激活,促进三羧酸循环,增强质子动力势,提高抗生素的摄取。
[Objective]we found that aminoglycosides can be used in combination with specific metabolites can effectively eliminate multidrug-resistant Vibro parahemolyticus.This works establishes a strategy for eradicating bacterial resistance that is based on metabolism.[Methods]we studied the phenotypic change and compared the percentage survival of multi-drug resistance V.parahemolyticus aftertreatment with different metabolites.Then we observed the transcription level changes of sucC,gltA,nuoF,nuoLandpfkAduring different infection time.[Results]Our results showed that when grown in the presence of 16μg/mlkanamycin,V.parahemolyticus cell survival decreased 64-,89-,1886-fold in the presence of glycerol,alanine,glucose.Treatment with CCCP was found to abolish aminoglycoside potentiation by all of the carbon sources.[Conclusion]this study suggest that bacterial metabolism is closely tied to aminoglycoside antibiotic susceptibility.The mechanism underlying this approach is that exogenous metabolites promotes the TCA cycle by substrate activation,which in turn increases proton motive force and stimulates uptake of antibiotic.

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