Immunomodulating Activities of Polysaccharides from ZhuQin Formula and Atractylodes in Hypoimmune Mice
|
摘要
[Objectives] To investigate the infrared identification of ZhuQin Formula and Atractylodes polysaccharides and its effect on immune function in immunocompromised mice. [Methods] In this experiment,the structure of polysaccharides was analyzed by Fourier transform infrared spectroscopy. The experiment was divided into the polysaccharide groups of high,medium and low dose of ZhuQin,the extraction group of ZhuQin,the polysaccharide group of Atractylodes,the positive drug group,the blank group and the model group. The model of immunosuppressed mice was established by intraperitoneal injection of cyclophosphamide. The carbon clearance index,immune organ index,serum hemolysin,spleen T and B lymphocyte transformation rate were determined. [Results]The results show that the polysaccharides have obvious absorption peaks of polysaccharides; Formula extracts and polysaccharides can enhance the immune function of immunosuppressive mice at a certain dose level,and the effect of formula polysaccharide is better than that of extract and single traditional Chinese medicine polysaccharides. [Conclusions] The results suggest that the polysaccharides of ZhuQin Formula and Atractylodes may be acidic polysaccharides containing pyran ring and furan ring. ZhuQin Formula polysaccharide may play an immunomodulatory role mainly by improving the specific immunity of the organism.
[Objectives] To investigate the infrared identification of ZhuQin Formula and Atractylodes polysaccharides and its effect on immune function in immunocompromised mice. [Methods] In this experiment,the structure of polysaccharides was analyzed by Fourier transform infrared spectroscopy. The experiment was divided into the polysaccharide groups of high,medium and low dose of ZhuQin,the extraction group of ZhuQin,the polysaccharide group of Atractylodes,the positive drug group,the blank group and the model group. The model of immunosuppressed mice was established by intraperitoneal injection of cyclophosphamide. The carbon clearance index,immune organ index,serum hemolysin,spleen T and B lymphocyte transformation rate were determined. [Results]The results show that the polysaccharides have obvious absorption peaks of polysaccharides; Formula extracts and polysaccharides can enhance the immune function of immunosuppressive mice at a certain dose level,and the effect of formula polysaccharide is better than that of extract and single traditional Chinese medicine polysaccharides. [Conclusions] The results suggest that the polysaccharides of ZhuQin Formula and Atractylodes may be acidic polysaccharides containing pyran ring and furan ring. ZhuQin Formula polysaccharide may play an immunomodulatory role mainly by improving the specific immunity of the organism.
[Objectives] To investigate the infrared identification of ZhuQin Formula and Atractylodes polysaccharides and its effect on immune function in immunocompromised mice. [Methods] In this experiment,the structure of polysaccharides was analyzed by Fourier transform infrared spectroscopy. The experiment was divided into the polysaccharide groups of high,medium and low dose of ZhuQin,the extraction group of ZhuQin,the polysaccharide group of Atractylodes,the positive drug group,the blank group and the model group. The model of immunosuppressed mice was established by intraperitoneal injection of cyclophosphamide. The carbon clearance index,immune organ index,serum hemolysin,spleen T and B lymphocyte transformation rate were determined. [Results]The results show that the polysaccharides have obvious absorption peaks of polysaccharides; Formula extracts and polysaccharides can enhance the immune function of immunosuppressive mice at a certain dose level,and the effect of formula polysaccharide is better than that of extract and single traditional Chinese medicine polysaccharides. [Conclusions] The results suggest that the polysaccharides of ZhuQin Formula and Atractylodes may be acidic polysaccharides containing pyran ring and furan ring. ZhuQin Formula polysaccharide may play an immunomodulatory role mainly by improving the specific immunity of the organism.
引文
[1]ZHAO WZ,YIN YG,YV ZP,et al.FTIR structure analysis and microwave extraction of polysaccharides from corn silk[J].Journal of Jilin University(Engineering and Technology Edition),2012(2):515-520.
[2]SUN XY,CUI ZY,ZHANG ML,et al.Effect of Lycium barbarum polysaccharides and pachymaran on immune function and intestinal mucosal system in immunosuppressive mice[J].Chinese Journal of Veterinary Science,2015(3):450-455.
[3]LEI H,QI CN.Chinese herbal medicine enhances immune organ function[J].Chinese Journal of Traditional Veterinary Science,2002(2):37-38.
[4]CHEN Y.Study on pattern recognition of ganoderma IR fingerprint and polysaccharide content in Ganoderma lucidum[D].Guangzhou:Guangdong University of Technology,2012.
[5]YV HJ,ZHANG QH,ZHANG D.Isolation and purification of basil polysaccharide and IR spectrscopy analysis[J].Journal of Liaoning University of Traditional Chinese Medicine,2016(7):66-68.
[6]ZHANG M,LI C,FEI JY,et al.Study on the purification and IR spectroscopy of se-polysaccharides in selenium enriched flammulina velutipes[J].Journal of Instrumental Analysis,2008,27(2):158-161.
[7]ZHANG SJ,LI WQ,DENG YZ,et al.Analysis of marine microalgal polysaccharides by gas chromatography and IR spectroscopy[J].Journal of Xiamen University(Natural Science),2005,44(s1):212-214.
[8]LI YY,XV QY,ZHENG WX,et al.Study of phascolosomaesculenta coelomic fluid polysaccharide[C].Annual meeting of National Society for Biochemical and Biotechnological Drugs,2007.
[9]LIU H,ZHANG J.Determination of molecular structure of carboxymethyl pumpkin polysaccharide by gas chromatography and IR spectroscopy[J].Chinese Journal of spectroscopy Laboratory,2008,25(3):313-318.
[10]HUO GH,LI LS,GAO YY.Application of spectral analysis in the structure analysis of polysaccharides[J].Chemistry of Life,2002,22(2):194-196.
[11]XUN ZM.Influence of zhuqin formula extract on the cellular and humoral immune response in cyclophamide-immunocompromised mice[A].Proceedings of the seventh China animal husbandry science and Technology Forum[C].Chinese Association of Animal Science and Veterinary Medicine,2016:1.
[12]SUN WP,LI FS,CHEN C,et al.Immunomodulation of Atractylodis polysaccharides in mice[J].Chinese Journal of Microecology,2011,23(10):881-882.
[13]WANG YF,LI T,TANG ZH,et al.Advances in studies on chemical constituents and pharmacological activities of Astragalus membranaceus[J].Chinese Archives of Traditional Chinese Medicine,2015,33(1):206-209.
[14]XU YT.Research development on chemical composition and modern pharmacology of Sastellaria baicalensis[J].Guangming traditional Chinese medicine,2010,25(3):544-545.
[15]LIU DL,BAO HY,LIU Y.Progress on chemical constituents and pharmacological effects of Astragali radix in recent five years[J].Food and Drug,2014,16(1):68-70.
[16]YOU X,XIONG DG,GUO ZB,et al.Advancement of a variety of chemical constituents and pharmacological mechanisms of Poria corns[J].Journal of Anhui Agricultural Sciences,2015,43(2):106-109.
[17]ZHANG XJ,ZUO DD,FAN Y.Research progress on chemical constituents,quality control and pharmacological effects of Poria cocos[J].Information on Traditional Chinese Medicine,2014,31(1):117-119.
[18]ZHANG ZG.Optimization of extraction process of polysaccharide for compound Chinese herbal medicine and effect of polysaccharide on the immune function in mice[D].Hefei:Anhui Agricultural University,2011.
[19]YANG XX,WANG Y,HAN ZA,et al.Interventional effect of water decoction of Epimedium on immune system model of mice at different levels[J].Journal of Changchun University of Chinese Medicine,2016(1):14-17.
[20]REN J,GU Y.Pharmacological study on immunoregulation effect of Shenqi polysaccharide effervescent tablets[J].Modern Medicine&Health,2016(1):51-53.
[21]ZHU C,YANG MQ,ZHAO SZ.Effects of the secondary metabolites of rhizotonia leguminicola on immune functions of immunosuppressed mice[J].Chinese Journal of Immunology,2010,26(5):51-53.
[22]YAN ZQ,ZHU ZR,LIU J,et al.Effects of total polysaccharides of Kuqin compound on immune function of immunosuppressive mice[J].Chinese Journal of Veterinary Medicine,2014(8):85-87.
[23]ZHANG WY,YANG Y,CHEN CL,et al.Effect of Poria power on immune function of immunosuppressive mice[J].Chinese Journal of Veterinary Science,2014,34(2):283-287.
[24]ZHAO X,HE GL,LU CY,et al.Immunoregulation effect of rhizoma drynariae flavonoids on immunosuppressive mice[J].Natural Product Research and Development,2016(28):438-445.
[25]DUAN YQ,CHENG YX,CHENG R,et al.Variation of the specific and nonspecific immune function of mice with spleen deficiency and effect of Chinese medicine[J].Chinese Journal of Gerontology,2011(31):2874-2876.
[2]SUN XY,CUI ZY,ZHANG ML,et al.Effect of Lycium barbarum polysaccharides and pachymaran on immune function and intestinal mucosal system in immunosuppressive mice[J].Chinese Journal of Veterinary Science,2015(3):450-455.
[3]LEI H,QI CN.Chinese herbal medicine enhances immune organ function[J].Chinese Journal of Traditional Veterinary Science,2002(2):37-38.
[4]CHEN Y.Study on pattern recognition of ganoderma IR fingerprint and polysaccharide content in Ganoderma lucidum[D].Guangzhou:Guangdong University of Technology,2012.
[5]YV HJ,ZHANG QH,ZHANG D.Isolation and purification of basil polysaccharide and IR spectrscopy analysis[J].Journal of Liaoning University of Traditional Chinese Medicine,2016(7):66-68.
[6]ZHANG M,LI C,FEI JY,et al.Study on the purification and IR spectroscopy of se-polysaccharides in selenium enriched flammulina velutipes[J].Journal of Instrumental Analysis,2008,27(2):158-161.
[7]ZHANG SJ,LI WQ,DENG YZ,et al.Analysis of marine microalgal polysaccharides by gas chromatography and IR spectroscopy[J].Journal of Xiamen University(Natural Science),2005,44(s1):212-214.
[8]LI YY,XV QY,ZHENG WX,et al.Study of phascolosomaesculenta coelomic fluid polysaccharide[C].Annual meeting of National Society for Biochemical and Biotechnological Drugs,2007.
[9]LIU H,ZHANG J.Determination of molecular structure of carboxymethyl pumpkin polysaccharide by gas chromatography and IR spectroscopy[J].Chinese Journal of spectroscopy Laboratory,2008,25(3):313-318.
[10]HUO GH,LI LS,GAO YY.Application of spectral analysis in the structure analysis of polysaccharides[J].Chemistry of Life,2002,22(2):194-196.
[11]XUN ZM.Influence of zhuqin formula extract on the cellular and humoral immune response in cyclophamide-immunocompromised mice[A].Proceedings of the seventh China animal husbandry science and Technology Forum[C].Chinese Association of Animal Science and Veterinary Medicine,2016:1.
[12]SUN WP,LI FS,CHEN C,et al.Immunomodulation of Atractylodis polysaccharides in mice[J].Chinese Journal of Microecology,2011,23(10):881-882.
[13]WANG YF,LI T,TANG ZH,et al.Advances in studies on chemical constituents and pharmacological activities of Astragalus membranaceus[J].Chinese Archives of Traditional Chinese Medicine,2015,33(1):206-209.
[14]XU YT.Research development on chemical composition and modern pharmacology of Sastellaria baicalensis[J].Guangming traditional Chinese medicine,2010,25(3):544-545.
[15]LIU DL,BAO HY,LIU Y.Progress on chemical constituents and pharmacological effects of Astragali radix in recent five years[J].Food and Drug,2014,16(1):68-70.
[16]YOU X,XIONG DG,GUO ZB,et al.Advancement of a variety of chemical constituents and pharmacological mechanisms of Poria corns[J].Journal of Anhui Agricultural Sciences,2015,43(2):106-109.
[17]ZHANG XJ,ZUO DD,FAN Y.Research progress on chemical constituents,quality control and pharmacological effects of Poria cocos[J].Information on Traditional Chinese Medicine,2014,31(1):117-119.
[18]ZHANG ZG.Optimization of extraction process of polysaccharide for compound Chinese herbal medicine and effect of polysaccharide on the immune function in mice[D].Hefei:Anhui Agricultural University,2011.
[19]YANG XX,WANG Y,HAN ZA,et al.Interventional effect of water decoction of Epimedium on immune system model of mice at different levels[J].Journal of Changchun University of Chinese Medicine,2016(1):14-17.
[20]REN J,GU Y.Pharmacological study on immunoregulation effect of Shenqi polysaccharide effervescent tablets[J].Modern Medicine&Health,2016(1):51-53.
[21]ZHU C,YANG MQ,ZHAO SZ.Effects of the secondary metabolites of rhizotonia leguminicola on immune functions of immunosuppressed mice[J].Chinese Journal of Immunology,2010,26(5):51-53.
[22]YAN ZQ,ZHU ZR,LIU J,et al.Effects of total polysaccharides of Kuqin compound on immune function of immunosuppressive mice[J].Chinese Journal of Veterinary Medicine,2014(8):85-87.
[23]ZHANG WY,YANG Y,CHEN CL,et al.Effect of Poria power on immune function of immunosuppressive mice[J].Chinese Journal of Veterinary Science,2014,34(2):283-287.
[24]ZHAO X,HE GL,LU CY,et al.Immunoregulation effect of rhizoma drynariae flavonoids on immunosuppressive mice[J].Natural Product Research and Development,2016(28):438-445.
[25]DUAN YQ,CHENG YX,CHENG R,et al.Variation of the specific and nonspecific immune function of mice with spleen deficiency and effect of Chinese medicine[J].Chinese Journal of Gerontology,2011(31):2874-2876.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |