桃红四物汤HPLC指纹图谱的总量统计矩及一次稳态投料量分析
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摘要
目的:建立中药及经典名方HPLC指纹图谱总量统计矩、信息量及一次投料量数学模型并进行实验验证。方法:运用总量统计矩原理和Hardy-Weinberg平衡群体一次稳态投料量计算方法,对来自不同产地的15批桃红四物汤原药材、饮片及复方制剂总量统计矩及一次稳态投料量进行研究。结果:除了总量一阶矩外,各药材、饮片及复方总量零阶矩、总量二阶矩、信息熵、信息量的RSD均> 10%。用单味原药材投料,除当归外(142. 34 kg),按全方累积的信息量的RSD变化计算出的一次稳态投料量最大(59. 10 kg);而按全方的比例投料,全方一次稳态投料量最大(958. 57 kg)。用炮制品投料,除酒红花外(83. 28 kg),全方累积的一次稳态投料量最大(73. 18 kg);按全方投料,炮制品一次稳态处方投料量均与原药材相同。原药材或炮制品组成的复方,其一次稳态投料量经常大于单味药材的一次稳态投料量。结论:中药成分受遗传多态性控制,中药色谱作用特征(成分种类)相近而作用强度(成分含量)不同,总量一阶矩、信息熵稳定说明中药多成分体系总体化学结构的印迹作用趋势稳定;桃红四物汤各药材及复方一次稳态投料量均远大于处方量,可为工业化大生产提供借鉴。
Objective: To establish a mathematical model of total statistical moment,information amount and one-time steady-state feeding quantity of HPLC fingerprint of traditional Chinese medicines(TCMs) and their classical prescriptions,and to carry out experimental verification. Method: The total statistical moment principle,Hardy-Weinberg equilibrium principle and one-time steady-state feeding quantity calculation method were used to study the total statistical moment parameters and one-time steady-state feeding quantity of 15 batches of original medicinal materials,decoction pieces and compound preparations of Taohong Siwutang,these original medicinal materials were from three producing areas. Result: In addition to the total first-order moment,RSD of total zeroorder moment,total second-order moment,information entropy and information amount of each medicinal material,decoction piece and compound were all greater than 10%. In the case of single original medicinal material,except for Angelicae Sinensis Radix(142. 34 kg),the one-time steady-state feeding quantity calculated according to the RSD change of the accumulated information amount of the whole prescription was the largest(59. 10 kg),and according to ratio of the whole prescription,one-time steady-state feeding quantity was the largest(958. 57 kg). In the case of processed products,the one-time steady-state feeding quantity of the whole prescription was the largest(73. 18 kg) except for Carthami Flos processed with wine(83. 28 kg),while according to the whole prescription,one-time steady-state feeding quantity of each processed products was the same as that of the original medicinal material. The one-time steady-state feeding quantity of the compound consisted of the original medicinal materials or processed products was often higher than that of the single medicinal material. Conclusion: The components of TCM are controlled by genetic polymorphism,the chromatographic characteristics(types of components) of Chinese medicine is similar and the intensity of action(content of components) is different. The stability of total first-order moment and information entropy indicates that the overall chemical structure of TCM multi-component system has a stable imprinting trend. The one-time steady-state feeding quantities of each medicinal material in Taohong Siwutang and this compound are far greater than the prescription amount,which can provide reference for the industrial production.
Objective: To establish a mathematical model of total statistical moment,information amount and one-time steady-state feeding quantity of HPLC fingerprint of traditional Chinese medicines(TCMs) and their classical prescriptions,and to carry out experimental verification. Method: The total statistical moment principle,Hardy-Weinberg equilibrium principle and one-time steady-state feeding quantity calculation method were used to study the total statistical moment parameters and one-time steady-state feeding quantity of 15 batches of original medicinal materials,decoction pieces and compound preparations of Taohong Siwutang,these original medicinal materials were from three producing areas. Result: In addition to the total first-order moment,RSD of total zeroorder moment,total second-order moment,information entropy and information amount of each medicinal material,decoction piece and compound were all greater than 10%. In the case of single original medicinal material,except for Angelicae Sinensis Radix(142. 34 kg),the one-time steady-state feeding quantity calculated according to the RSD change of the accumulated information amount of the whole prescription was the largest(59. 10 kg),and according to ratio of the whole prescription,one-time steady-state feeding quantity was the largest(958. 57 kg). In the case of processed products,the one-time steady-state feeding quantity of the whole prescription was the largest(73. 18 kg) except for Carthami Flos processed with wine(83. 28 kg),while according to the whole prescription,one-time steady-state feeding quantity of each processed products was the same as that of the original medicinal material. The one-time steady-state feeding quantity of the compound consisted of the original medicinal materials or processed products was often higher than that of the single medicinal material. Conclusion: The components of TCM are controlled by genetic polymorphism,the chromatographic characteristics(types of components) of Chinese medicine is similar and the intensity of action(content of components) is different. The stability of total first-order moment and information entropy indicates that the overall chemical structure of TCM multi-component system has a stable imprinting trend. The one-time steady-state feeding quantities of each medicinal material in Taohong Siwutang and this compound are far greater than the prescription amount,which can provide reference for the industrial production.
引文
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[2]贺福元,周宏灏,罗杰英,等.生物遗传多态性规律揭示中药材质量稳定性规律的探讨[J].中草药,2008,39(1):2-6,12.
[3]贺福元,邓凯文,刘文龙,等.中药复方对人体作用本质:“网通虹势”的多重遗传谱效动力学[J].中国实验方剂学杂志,2011,17(2):240-247.
[4]贺福元,周宏灏,罗杰英,等.谨防中药材GAP忽视生物遗传多态性规律的研究[J].湖南中医药大学学报,2007,27(S1):205-211.
[5]贺福元,周宏灏,邓凯文,等.指纹图谱的一种定性定量研究新方法:总量统计矩分析法[J].药学学报,2008,43(2):195-201.
[6]贺福元,邓凯文,黄胜,等.中药群体指纹图谱信息量和一次投料量数学模型的建立及对大黄和鱼腥草实验研究[J].中成药,2011,33(8):1292-1298.
[7]刘昌孝,陈士林,肖小河,等.中药质量标志物(Q-Marker):中药产品质量控制的新概念[J].中草药,2016,47(9):1443-1457.
[8]王飞龙,韩岚,樊玲,等.桃红四物汤对实验性脑缺血大鼠血清中ET-1,Ang-1,VEGF的影响[J].中国实验方剂学杂志,2017,23(1):101-106.
[9]张正升,彭代银.桃红四物汤临床应用研究进展[J].安徽医药,2011,15(9):1162-1165.
[10]国家药典委员会.中华人民共和国药典.四部[M].北京:中国医药科技出版社,2015:31.
[11]杨立伟,王海南,耿莲,等.基于标准汤剂的中药整体质量控制模式探讨[J].中国实验方剂学杂志,2018,24(8):1-6.
[12]刘文龙,张喜利,贺福元,等.稳态提取工艺中平衡群体的研究及对鱼腥草的实验验证[J].中国中药杂志,2011,36(21):2950-2955.
[13]LIU W L,ZHANG X L,FAN S Q,et al.A novel concept of Q-markers:molecular connectivity index[J].Phytomedicine,2018,45:36-40.
[14]肖美凤,张雨恬,杨岩涛,等.基于鱼腥草挥发性成分动态性的中药制剂“印迹模板”一致性分析[J].中国实验方剂学杂志,2019,doi:10.13422/j.cnki.syfjx.20190446.