Study on screening potential allergenic proteins from infant milk powders based on human mast cell membrane chromatography and histamine release assays
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摘要
Cow's milk allergy is mainly observed in infants and young children. Most allergic reactions affect the skin, followed by the gastrointestinal and respiratory systems. Conventional diagnosis is based on positive allergy studies and evaluation of parameters including IgE and IgG1 levels, acute allergic skin response and anaphylactic shock reactions. We developed a cell membrane chromatographic(CMC)method based on human mast cells(HMC-1) for screening potential allergens in infant formula milk powders(IFMP). HMC-1 cell membranes were extracted and mixed with silica to prepare cell membrane chromatography columns(10 mm ? 2 mm i.d., 5 mm). Under the conditions of 0.2 mL/min flow rate and214 nm detection wavelength, human breast milk showed no retention. However, IFMP showed clear retention. The retained fractions were collected and analyzed through matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS). Four major milk proteins, i.e., α-casein, β-casein, α-lactalbumin, and β-lactoglobulin A, were identified. Furthermore, these proteins and β-lactoglobulin B showed clear retention on HMC-1/CMC columns. To test the degranulation effects of the five proteins, histamine and β-hexosaminidase release assays were carried out. All five proteins induced HMC-1 cells to release histamine and β-hexosaminidase. Also, we established a reversed phase liquid chromatographic(RPLC) method for the determination of the five proteins in IFMP and the results showed that 90% proteins in IFMP were α-casein and β-casein. We concluded that cow's milk proteins may be potential allergens and caseins cause more β-casein allergic risk than other proteins. This conclusion was consistent with other studies.
Cow's milk allergy is mainly observed in infants and young children. Most allergic reactions affect the skin, followed by the gastrointestinal and respiratory systems. Conventional diagnosis is based on positive allergy studies and evaluation of parameters including IgE and IgG1 levels, acute allergic skin response and anaphylactic shock reactions. We developed a cell membrane chromatographic(CMC)method based on human mast cells(HMC-1) for screening potential allergens in infant formula milk powders(IFMP). HMC-1 cell membranes were extracted and mixed with silica to prepare cell membrane chromatography columns(10 mm ? 2 mm i.d., 5 mm). Under the conditions of 0.2 mL/min flow rate and214 nm detection wavelength, human breast milk showed no retention. However, IFMP showed clear retention. The retained fractions were collected and analyzed through matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS). Four major milk proteins, i.e., α-casein, β-casein, α-lactalbumin, and β-lactoglobulin A, were identified. Furthermore, these proteins and β-lactoglobulin B showed clear retention on HMC-1/CMC columns. To test the degranulation effects of the five proteins, histamine and β-hexosaminidase release assays were carried out. All five proteins induced HMC-1 cells to release histamine and β-hexosaminidase. Also, we established a reversed phase liquid chromatographic(RPLC) method for the determination of the five proteins in IFMP and the results showed that 90% proteins in IFMP were α-casein and β-casein. We concluded that cow's milk proteins may be potential allergens and caseins cause more β-casein allergic risk than other proteins. This conclusion was consistent with other studies.
Cow's milk allergy is mainly observed in infants and young children. Most allergic reactions affect the skin, followed by the gastrointestinal and respiratory systems. Conventional diagnosis is based on positive allergy studies and evaluation of parameters including IgE and IgG1 levels, acute allergic skin response and anaphylactic shock reactions. We developed a cell membrane chromatographic(CMC)method based on human mast cells(HMC-1) for screening potential allergens in infant formula milk powders(IFMP). HMC-1 cell membranes were extracted and mixed with silica to prepare cell membrane chromatography columns(10 mm ? 2 mm i.d., 5 mm). Under the conditions of 0.2 mL/min flow rate and214 nm detection wavelength, human breast milk showed no retention. However, IFMP showed clear retention. The retained fractions were collected and analyzed through matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS). Four major milk proteins, i.e., α-casein, β-casein, α-lactalbumin, and β-lactoglobulin A, were identified. Furthermore, these proteins and β-lactoglobulin B showed clear retention on HMC-1/CMC columns. To test the degranulation effects of the five proteins, histamine and β-hexosaminidase release assays were carried out. All five proteins induced HMC-1 cells to release histamine and β-hexosaminidase. Also, we established a reversed phase liquid chromatographic(RPLC) method for the determination of the five proteins in IFMP and the results showed that 90% proteins in IFMP were α-casein and β-casein. We concluded that cow's milk proteins may be potential allergens and caseins cause more β-casein allergic risk than other proteins. This conclusion was consistent with other studies.
引文
[1]R.B.Canani,R.Nocerino,G.Terrin,et al.,Formula selection for management of children with cow's milk allergy influences the rate of acquisition of tolerance:a prospective multicenter study,J.Pediatr.163(2013)771-777.
[2]A.Katz,N.V.Hundal,Q.Yuan,et al.,Cow's milk allergy:a new approach needed?J.Pediatr.163(2013)620-622.
[3]W.N.Eigel,J.E.Butler,C.A.Ernstrom,et al.,Nomenclature of proteins of cow's milk:fifth revision,J.Dairy Sci.67(1984)1599-1631.
[4]O.Borrelli,V.Mancini,N.Thapar,et al.,Cow's milk challenge increases weakly acidic reflux in children with cow's milk allergy and gastroesophageal reflux disease,J.Pediatr.161(2012)476-481.
[5]A.M.Aragones,L.E.Zudaire,E.A.Lebrero,et al.,Food allergy committee of,position document:IgE-mediated cow's milk allergy,Allergol.Immunopathol.43(2015)507-526.
[6]A.P.Castro,A.C.Pastorino,A.K.F.Gushken,et al.,Establishing a cut-off for the serum levels of specific IgE to milk and its components for cow's milk allergy:results from a specific population,Allergol.Immunopathol.43(2015)67-72.
[7]R.Fritsché,Animal models in food allergy:assessment of allergenicity and preventive activity of infant formulas,Toxicol.Lett.140-141(2003)303-309.
[8]L.Mayer,Mucosal immunity,Pediatrics 111(2003)1595-1600.
[9]A.Elizur,N.Rajuan,M.R.Goldberg,et al.,Natural course and risk factors for persistence of IgE-mediated cow's milk allergy,J.Pediatr.161(2012)482-487.
[10]X.Hou,S.Wang,T.Zhang,et al.,Recent advances in cell membrane chromatography for traditional Chinese medicines analysis,J.Pharm.Biomed.Anal.101(2014)141-150.
[11]T.J.Smith,S.J.Parikh,HMC-1 mast cells activate human orbital fibroblasts in coculture:evidence for up-regulation of prostaglandin E2 and hyaluronan synthesis,Endocrinology 140(1999)3518-3525.
[12]S.Han,T.Zhang,J.Huang,et al.,New method of screening allergenic components from Shuanghuanglian injection:with RBL-2H3/CMC model online HPLC/MS system,J.Pharm.Biomed.Anal.88(2014)602-608.
[13]J.Ma,B.Zhang,Y.Wang,et al.,Determination of flavor enhancers in milk powder by one-step sample preparation and two-dimensional liquid chromatography,J.Sep.Sci.37(2014)920-926.
[14]X.Hou,M.Zhou,Q.Jiang,et al.,A vascular smooth muscle/cell membrane chromatography-offline-gas chromatography/mass spectrometry method for recognition,separation and identification of active components from traditional Chinese medicines,J.Chromatogr.A 1216(2009)7081-7087.
[15]Y.Cao,X.F.Chen,D.Y.Lü,et al.,Using cell membrane chromatography and HPLC-TOF/MS method for in vivo study of active components from roots of Aconitum carmichaeli,J.Pharm.Anal.1(2011)125-134.
[16]H.Tachibana,Y.Sunada,T.Miyase,et al.,Identification of a methylated tea catechin as an inhibitor of degranulation in human basophilic KU812 cells,Biosci.Biotechnol.Biochem.64(2000)452-454.
[17]K.C.Chimalakonda,E.Pang,J.L.Weaver,et al.,Development and validation of a liquid-chromatography tandem mass spectrometry method to determine in vitro and in vivo histamine release,J.Pharm.Biomed.Anal.102(2015)494-499.
[18]R.H.B.Chunduri,G.S.Dannana,Development and validation of a high throughput UPLC-MS/MS method for simultaneous quantification of esomeprazole,rabeprazole and levosulpiride in human plasma,J.Pharm.Anal.6(2016)190-198.
[19]X.F.Hou,X.Yuan,B.Zhang,et al.,Screening active anti-breast cancer compounds from Cortex Magnolia officinalis by 2D LC-MS,J.Sep.Sci.36(2013)706-712.
[20]G.Nilsson,T.Blom,M.Kusche-Gullberg,et al.,Phenotypic characterization of the human mast-cell line HMC-1,Scand.J.Immunol.39(1994)489-498.
[21]Y.D.Min,C.H.Choi,H.Bark,et al.,Quercetin inhibits expression of inflammatory cytokines through attenuation of NF-kappaB and p38 MAPK in HMC-1 human mast cell line,Inflamm.Res.56(2007)210-215.
[22]G.Johnsson,C.G.Reg?rdh,L.S?lvell,Combined pharmacokinetic and pharmacodynamic studies in man of the adrenergicβ1-receptor antagonist metoprolol,Acta Pharmacol.Toxicol.36(2009)31-44.
[23]Y.Nakamura,M.Oka,H.Soda,et al.,Gefitinib("Iressa",ZD1839),an epidermal growth factor receptor tyrosine kinase inhibitor,reverses breast cancer resistance protein/ABCG2-mediated drug resistance,Cancer Res.65(2005)1541-1546.
[24]V.Gross,A.Lippoldt,W.Schneider,et al.,Effect of captopril and angiotensin IIreceptor blockade on pressure natriuresis in transgenic TGR(mRen-2)27 rats,Hypertension 26(1995)471-479.
[25]E.Savilahti,Cow's milk allergy,Allergy 36(1981)73-88.
[26]N.Gasilova,H.H.Girault,Component-resolved diagnostic of cow's milk allergy by immunoaffinity capillary electrophoresis-matrix assisted laser desorption/ionization mass spectrometry,Anal.Chem.86(2014)6337-6345.
[27]E.A.Permyakov,L.J.Berliner,α-Lactalbumin:structure and function,FEBS Lett.473(2000)269-274.
[28]H.Y.Lam,E.van Hoffen,A.Michelsen,et al.,Cow's milk allergy in adults is rare but severe:both casein and whey proteins are involved,Clin.Exp.Allergy 38(2008)995-1002.
[29]S.Sharma,P.Kumar,C.Betzel,et al.,Structure and function of proteins involved in milk allergies,J.Chromatogr.B 756(2001)183-187.
[30]M.L.Burr,Does infant feeding affect the risk of allergy?Arch.Dis.Child.58(1983)561-565.
[31]A.S.Goldman,W.A.Sellars,S.R.Halpern,et al.,Milk allergy.II.Skin testing of allergic and normal children with purified milk proteins,Pediatrics 32(1963)572-579.
[32]G.H.Docena,R.Fernandez,F.G.Chirdo,et al.,Identification of casein as the major allergenic and antigenic protein of cow's milk,Allergy 51(1996)412-416.
[33]V.Payne,P.C.Kam,Mast cell tryptase:a review of its physiology and clinical significance,Anaesthesia 59(2004)695-703.
[34]R.Kojima,T.Ohno,M.Iikura,et al.,Galectin-9 enhances cytokine secretion,but suppresses survival and degranulation,in human mast cell line,PLoS One9(2014)e86106.
[35]O.H.Kang,H.J.An,S.B.Kim,et al.,Tetrandrine suppresses pro-inflammatory mediators in PMA plus A23187-induced HMC-1 cells,Int.J.Mol.Med.33(2014)1335-1340.
[36]J.Ma,X.Hou,B.Zhang,et al.,The analysis of carbohydrates in milk powder by a new"heart-cutting"two-dimensional liquid chromatography method,J.Pharm.Biomed.Anal.91(2014)24-31.
[2]A.Katz,N.V.Hundal,Q.Yuan,et al.,Cow's milk allergy:a new approach needed?J.Pediatr.163(2013)620-622.
[3]W.N.Eigel,J.E.Butler,C.A.Ernstrom,et al.,Nomenclature of proteins of cow's milk:fifth revision,J.Dairy Sci.67(1984)1599-1631.
[4]O.Borrelli,V.Mancini,N.Thapar,et al.,Cow's milk challenge increases weakly acidic reflux in children with cow's milk allergy and gastroesophageal reflux disease,J.Pediatr.161(2012)476-481.
[5]A.M.Aragones,L.E.Zudaire,E.A.Lebrero,et al.,Food allergy committee of,position document:IgE-mediated cow's milk allergy,Allergol.Immunopathol.43(2015)507-526.
[6]A.P.Castro,A.C.Pastorino,A.K.F.Gushken,et al.,Establishing a cut-off for the serum levels of specific IgE to milk and its components for cow's milk allergy:results from a specific population,Allergol.Immunopathol.43(2015)67-72.
[7]R.Fritsché,Animal models in food allergy:assessment of allergenicity and preventive activity of infant formulas,Toxicol.Lett.140-141(2003)303-309.
[8]L.Mayer,Mucosal immunity,Pediatrics 111(2003)1595-1600.
[9]A.Elizur,N.Rajuan,M.R.Goldberg,et al.,Natural course and risk factors for persistence of IgE-mediated cow's milk allergy,J.Pediatr.161(2012)482-487.
[10]X.Hou,S.Wang,T.Zhang,et al.,Recent advances in cell membrane chromatography for traditional Chinese medicines analysis,J.Pharm.Biomed.Anal.101(2014)141-150.
[11]T.J.Smith,S.J.Parikh,HMC-1 mast cells activate human orbital fibroblasts in coculture:evidence for up-regulation of prostaglandin E2 and hyaluronan synthesis,Endocrinology 140(1999)3518-3525.
[12]S.Han,T.Zhang,J.Huang,et al.,New method of screening allergenic components from Shuanghuanglian injection:with RBL-2H3/CMC model online HPLC/MS system,J.Pharm.Biomed.Anal.88(2014)602-608.
[13]J.Ma,B.Zhang,Y.Wang,et al.,Determination of flavor enhancers in milk powder by one-step sample preparation and two-dimensional liquid chromatography,J.Sep.Sci.37(2014)920-926.
[14]X.Hou,M.Zhou,Q.Jiang,et al.,A vascular smooth muscle/cell membrane chromatography-offline-gas chromatography/mass spectrometry method for recognition,separation and identification of active components from traditional Chinese medicines,J.Chromatogr.A 1216(2009)7081-7087.
[15]Y.Cao,X.F.Chen,D.Y.Lü,et al.,Using cell membrane chromatography and HPLC-TOF/MS method for in vivo study of active components from roots of Aconitum carmichaeli,J.Pharm.Anal.1(2011)125-134.
[16]H.Tachibana,Y.Sunada,T.Miyase,et al.,Identification of a methylated tea catechin as an inhibitor of degranulation in human basophilic KU812 cells,Biosci.Biotechnol.Biochem.64(2000)452-454.
[17]K.C.Chimalakonda,E.Pang,J.L.Weaver,et al.,Development and validation of a liquid-chromatography tandem mass spectrometry method to determine in vitro and in vivo histamine release,J.Pharm.Biomed.Anal.102(2015)494-499.
[18]R.H.B.Chunduri,G.S.Dannana,Development and validation of a high throughput UPLC-MS/MS method for simultaneous quantification of esomeprazole,rabeprazole and levosulpiride in human plasma,J.Pharm.Anal.6(2016)190-198.
[19]X.F.Hou,X.Yuan,B.Zhang,et al.,Screening active anti-breast cancer compounds from Cortex Magnolia officinalis by 2D LC-MS,J.Sep.Sci.36(2013)706-712.
[20]G.Nilsson,T.Blom,M.Kusche-Gullberg,et al.,Phenotypic characterization of the human mast-cell line HMC-1,Scand.J.Immunol.39(1994)489-498.
[21]Y.D.Min,C.H.Choi,H.Bark,et al.,Quercetin inhibits expression of inflammatory cytokines through attenuation of NF-kappaB and p38 MAPK in HMC-1 human mast cell line,Inflamm.Res.56(2007)210-215.
[22]G.Johnsson,C.G.Reg?rdh,L.S?lvell,Combined pharmacokinetic and pharmacodynamic studies in man of the adrenergicβ1-receptor antagonist metoprolol,Acta Pharmacol.Toxicol.36(2009)31-44.
[23]Y.Nakamura,M.Oka,H.Soda,et al.,Gefitinib("Iressa",ZD1839),an epidermal growth factor receptor tyrosine kinase inhibitor,reverses breast cancer resistance protein/ABCG2-mediated drug resistance,Cancer Res.65(2005)1541-1546.
[24]V.Gross,A.Lippoldt,W.Schneider,et al.,Effect of captopril and angiotensin IIreceptor blockade on pressure natriuresis in transgenic TGR(mRen-2)27 rats,Hypertension 26(1995)471-479.
[25]E.Savilahti,Cow's milk allergy,Allergy 36(1981)73-88.
[26]N.Gasilova,H.H.Girault,Component-resolved diagnostic of cow's milk allergy by immunoaffinity capillary electrophoresis-matrix assisted laser desorption/ionization mass spectrometry,Anal.Chem.86(2014)6337-6345.
[27]E.A.Permyakov,L.J.Berliner,α-Lactalbumin:structure and function,FEBS Lett.473(2000)269-274.
[28]H.Y.Lam,E.van Hoffen,A.Michelsen,et al.,Cow's milk allergy in adults is rare but severe:both casein and whey proteins are involved,Clin.Exp.Allergy 38(2008)995-1002.
[29]S.Sharma,P.Kumar,C.Betzel,et al.,Structure and function of proteins involved in milk allergies,J.Chromatogr.B 756(2001)183-187.
[30]M.L.Burr,Does infant feeding affect the risk of allergy?Arch.Dis.Child.58(1983)561-565.
[31]A.S.Goldman,W.A.Sellars,S.R.Halpern,et al.,Milk allergy.II.Skin testing of allergic and normal children with purified milk proteins,Pediatrics 32(1963)572-579.
[32]G.H.Docena,R.Fernandez,F.G.Chirdo,et al.,Identification of casein as the major allergenic and antigenic protein of cow's milk,Allergy 51(1996)412-416.
[33]V.Payne,P.C.Kam,Mast cell tryptase:a review of its physiology and clinical significance,Anaesthesia 59(2004)695-703.
[34]R.Kojima,T.Ohno,M.Iikura,et al.,Galectin-9 enhances cytokine secretion,but suppresses survival and degranulation,in human mast cell line,PLoS One9(2014)e86106.
[35]O.H.Kang,H.J.An,S.B.Kim,et al.,Tetrandrine suppresses pro-inflammatory mediators in PMA plus A23187-induced HMC-1 cells,Int.J.Mol.Med.33(2014)1335-1340.
[36]J.Ma,X.Hou,B.Zhang,et al.,The analysis of carbohydrates in milk powder by a new"heart-cutting"two-dimensional liquid chromatography method,J.Pharm.Biomed.Anal.91(2014)24-31.