基于cyt b基因的假眼小绿叶蝉11个地理种群遗传分化研究
|
摘要
应用特异性引物扩增假眼小绿叶蝉(Empoasca vitis G?the)56个不同地理种群Cytb基因片段,探讨了11个地理种群间的遗传多样性、分子变异、遗传分化程度及基因流水平,测序结果表明56条序列存在236个变异位点,45个单倍型。分析得出:(1)11个地理种群群体单倍型多样度Hd为0. 978 79,群体的Hd范围为0. 933 3～1. 000 0,总群体和各种群的中性检验结果均不显著,其进化模型为中性模型。(2)总群体遗传分化系数Gst与固定系数Fst分别为0. 017 03与0. 165 47。种群间基因流Nm为1. 26,较频繁基因交流减小了遗传差异,不存在明显的地理分化效应。
The partial mtDNA Cytb gene sequences of of 11 populations of Empoasca vitis(G?the)were compared.The genetic structure of these populations was further investigated based on the cyt b DNA sequences. A total of 56 cyt b gene partial sequences with 236 variable sites and 45 haplotypes were obtained. The haplotype diversity index(Hd)was0. 978 79 among all populations,ranging from 0. 933 3 to 1. 000 0 within single population. The Tajima's D tests for all populations or each single population did not reach any significant level,which suggest that the evolution and the abundance of Empoasca vitis(G?the)tea plantations remained on quantitative level,which fits well with the neutral model.Among the 11 populations,the total genetic differentiation coefficient(Gst),total fixation index(Fst)and gene flow(Nm)were 0. 017 03,0. 165 47,and 1. 26,respectively. These indicate that the high level of gene exchange does exist in the different geographical populations,but not in genetic differentiation.
The partial mtDNA Cytb gene sequences of of 11 populations of Empoasca vitis(G?the)were compared.The genetic structure of these populations was further investigated based on the cyt b DNA sequences. A total of 56 cyt b gene partial sequences with 236 variable sites and 45 haplotypes were obtained. The haplotype diversity index(Hd)was0. 978 79 among all populations,ranging from 0. 933 3 to 1. 000 0 within single population. The Tajima's D tests for all populations or each single population did not reach any significant level,which suggest that the evolution and the abundance of Empoasca vitis(G?the)tea plantations remained on quantitative level,which fits well with the neutral model.Among the 11 populations,the total genetic differentiation coefficient(Gst),total fixation index(Fst)and gene flow(Nm)were 0. 017 03,0. 165 47,and 1. 26,respectively. These indicate that the high level of gene exchange does exist in the different geographical populations,but not in genetic differentiation.
引文
[1]Zheng Y T,Wang M X,Cui L,et al.Resistance of tea cultivars to the tea green leafhopper analyzed by EPGtechnique and their resistancerelated substances[J].Acta Ecologica Sinica,2017,37(23):80158028.郑雨婷,王梦馨,崔林,等.基于EPG技术分析茶树品种对茶小绿叶蝉的抗性及其相关的抗性物质[J].生态学报,2017,37(23):80158028.
[2]Pan C,Lin J L,Han B Y,et al.Evaluation of attraction of volatile semiochemicals from tea shoots to the mymarid Stethynium empoascae[J].Acta Ecologica Sinica,2016,36(12):37853795.潘铖,林金丽,韩宝瑜,等.茶梢信息物引诱叶蝉三棒缨小蜂效应的检测[J].生态学报,2016,36(12):37853795.
[3]Han S J,Pan Y,Han B Y,et al.Changes in volatiles of tea shoots damaged by tea green leafhoppers and their attraction to Schizophragma parvula Ogloblin[J].Chinese Journal of Biological Control,2016,32(2):142148.韩善捷,潘钺,韩宝瑜,等.假眼小绿叶蝉为害致茶梢挥发物变化及其引诱微小裂骨缨小蜂效应[J].中国生物防治学报,2016,32(2):142148.
[4]Cui L,Yu P F,Han S J,et al.Effects of tea plantation environment,cultivation and cultivar on the population density of tea green leafhopper[J].Journal of Anhui Agricultural University,2017,44(6):963967.崔林,俞鹏飞,韩善捷,等.茶园环境和管理技术及茶树品种对假眼小绿叶蝉密度的影响[J].安徽农业大学学报,2017,44(6):963967.
[5]Yu Y,Wang Z X,Li S,et al.Degree of close spatialfollowing relationship between four insect pests and their natural enemies studied in a span of two years about"WuNiuZao"tea garden[J].Acta Ecologica Sinica,2018,38(13):48174833.余燕,王振兴,李尚,等.天敌对"乌牛早"茶园4种害虫空间跟随关系密切程度的年度差异[J].生态学报,2018,38(13):48174833.
[6]Meng Z N,Bian L,Luo Z X,et al.Taxonomic revision and analysis of the green tea leafhopper species in China's main tea production area[J].Chinese Bulletin of Entomology,2018,55(3)514526.孟召娜,边磊,罗宗秀,等.全国主产茶区茶树小绿叶蝉种类鉴定及分析[J].应用昆虫学报,2018,55(3)514526.
[7]Fu J Y,Han B Y.Studies on genetic relationships among populations of Empoasca vitis(Gothe)from tea gardens in seven provinces based on RAPD analysis[J].Acta Argiculture Zhejiangensis,2007,19(1):1014.付建玉,韩宝瑜.七省茶园假眼小绿叶蝉的RAPD分析及其亲缘关系探讨[J].浙江农业学报,2007,19(1):1014.
[8]Fu J Y,Li L,Yuan Z J,et al.Molecular phylogenetic analysis of tea green leafhopper(Empoasca vitis)based on mtDNA CO I sequences[J].Journal of Tea Science,2014,34(6):601608.付建玉,李乐,袁志军,等.基于mtDNA CO I的假眼小绿叶蝉系统发育研究[J].茶叶科学,2014,34(6):601608.
[9]Dai J X,Zhang D Z.Phylogenetic analysis of eight species of Pimeliinae based on CO II and cyt b gene sequences[J].Entomological Knowledge,2008,(4):554558.代金霞,张大治.基于线粒体CO II和cyt b基因序列的8种漠甲系统发育关系[J].昆虫知识,2008,(4):554558.
[10]Qiao F,Chen Z N,Chen Z,et al.Phylogenetic relationships among seven Pieridae genera based on partial sequences of the CO I and cyt b genes[J].Chinese Journal of Applied Entomology,2014,51(1):127138.乔枫,陈振宁,陈志,等.基于线粒体CO I和cyt b基因序列研究粉蝶科七属间的系统发生关系[J].应用昆虫学报,2014,51(1):127138.
[11]Zhou N N.Genetic differentiation of geographical population of tea plantation pseudoeye leafhopper based on mitochondrial CO I and CO II genes[D].China Jiliang University,2014周宁宁.基于线粒体CO I和CO II基因的茶园假眼小绿叶蝉地理种群的遗传分化研究[D].中国计量学院,2014.
[12]Zhang Y,Skolnick J.TMalign:a protein structure alignment algorithm based on the TMscore[J].Nucleic Acids Res,2005,33(7):23022309.
[13]Cosson J F,Hutterer R,Libois R,et al.Phylogeographical footprints of the Strait of Gibraltar and Quaternary climatic fluctuations in the western Mediterranean:A case study with the greater whitetoothed shrew,Crocidura russula(Mammalia:Soricidae)[J].Mol Ecol,2005,14(4):11511162.
[14]Balloux F,LugonMoulin N.The estimation of population differentiation with microsatellite markers[J].Mol Ecol,2002,11(2):155165.
[15]Krebs R A.Combining paternally and maternally inherited mitochondrial DNA for analysis of population structure in mussels[J].Mol Ecol,2004,13(6):17011705.
[16]Zhou N N,Wang M X,Cui L,et al.Genetic variation of Empoasca vitis(G?the)(Hemiptera:Cicadellidae)among different geographical populations based on mtDNA CO I complete sequence[J].Acta Ecologica Sinica,2014,34(23):68796889.周宁宁,王梦馨,崔林,等.基于CO I基因全长序列的假眼小绿叶蝉地理种群遗传分化研究[J].生态学报,2014,34(23):68796889.
[17]Wei F W,Nie Y G,Miao H X,et al.Advancements of the researches on biodiversity loss mechanisms[J].Chinese Science Bulletin,2014,59(6):430437.魏辅文,聂永刚,苗海霞,等.生物多样性丧失机制研究进展[J].科学通报,2014,59(6):430437.
[18]Zhang L J,Shen D R,Sun Y X,et al.Analysis of genetic differentiation among geographic populations of Gynaikothrips ficorum(Marchal)(Thysanoptera:Phlaeothripidae)in Yunnan,southwestern China,based on mtDNA CO I gene sequences[J].Acta Entomologica Sinica,2012,55(2):199207.张利娟,沈登荣,孙跃先,等.基于mtDNA CO I基因序列的云南榕母管蓟马不同地理种群的遗传分化分析[J].昆虫学报,2012,55(2):199207.
[19]Teinlek P,Siripattarapravat K,Tirawattanawanich C.Genetic diversity analysis of Thai indigenous chickens based on complete sequences of mitochondrial DNA Dloop region[J].Asianaustralas J Anim Sci,2018,31(6):804811.
[20]Zheng J C,Sun S L,Yue X R,et al.Phylogeny and evolution of the cholesterol transporter NPC1 in insects[J].J Insect Physiol,2018,107:157166.
[21]Yang X M,Lu Y H.Population genetic structure of Helicoverpa armigera in Ningxia,Inner Mongolia and neighboring areas[J].Chinese Journal of Applied Entomology,2018,55(1):2531.杨现明,陆宴辉.基于线粒体DNA的宁夏、内蒙古及周边地区棉铃虫种群遗传结构[J].应用昆虫学报,2018,55(1):2531.
[22]Vleck L D V,Wright S.Evolution and the genetics of populations.vol.1.genetic and biometric foundations[J].Biometrics,1969,25(3):607.
[23]Fu J N,Han B Y,Xiao Q.Mitochondrial CO I and16sRNA evidence for a single species hypothesis of E.vitis,J.formosana and E.onukii in East Asia[J].PLoS One,2014,9(12):e115259.
[24]Meng Z H,Yang M F,Zhou Y F,et al.Molecular phylogenetic analysis of some species of cicadellinae based on mitochondrial cyt b gene[J].Guizhou Agricultural Sciences,2013,41(7):15.孟泽洪,杨茂发,周玉锋,等.基于cyt b基因序列的大叶蝉亚科部分种类分子系统学分析[J].贵州农业科学,2013,41(7):15.
[2]Pan C,Lin J L,Han B Y,et al.Evaluation of attraction of volatile semiochemicals from tea shoots to the mymarid Stethynium empoascae[J].Acta Ecologica Sinica,2016,36(12):37853795.潘铖,林金丽,韩宝瑜,等.茶梢信息物引诱叶蝉三棒缨小蜂效应的检测[J].生态学报,2016,36(12):37853795.
[3]Han S J,Pan Y,Han B Y,et al.Changes in volatiles of tea shoots damaged by tea green leafhoppers and their attraction to Schizophragma parvula Ogloblin[J].Chinese Journal of Biological Control,2016,32(2):142148.韩善捷,潘钺,韩宝瑜,等.假眼小绿叶蝉为害致茶梢挥发物变化及其引诱微小裂骨缨小蜂效应[J].中国生物防治学报,2016,32(2):142148.
[4]Cui L,Yu P F,Han S J,et al.Effects of tea plantation environment,cultivation and cultivar on the population density of tea green leafhopper[J].Journal of Anhui Agricultural University,2017,44(6):963967.崔林,俞鹏飞,韩善捷,等.茶园环境和管理技术及茶树品种对假眼小绿叶蝉密度的影响[J].安徽农业大学学报,2017,44(6):963967.
[5]Yu Y,Wang Z X,Li S,et al.Degree of close spatialfollowing relationship between four insect pests and their natural enemies studied in a span of two years about"WuNiuZao"tea garden[J].Acta Ecologica Sinica,2018,38(13):48174833.余燕,王振兴,李尚,等.天敌对"乌牛早"茶园4种害虫空间跟随关系密切程度的年度差异[J].生态学报,2018,38(13):48174833.
[6]Meng Z N,Bian L,Luo Z X,et al.Taxonomic revision and analysis of the green tea leafhopper species in China's main tea production area[J].Chinese Bulletin of Entomology,2018,55(3)514526.孟召娜,边磊,罗宗秀,等.全国主产茶区茶树小绿叶蝉种类鉴定及分析[J].应用昆虫学报,2018,55(3)514526.
[7]Fu J Y,Han B Y.Studies on genetic relationships among populations of Empoasca vitis(Gothe)from tea gardens in seven provinces based on RAPD analysis[J].Acta Argiculture Zhejiangensis,2007,19(1):1014.付建玉,韩宝瑜.七省茶园假眼小绿叶蝉的RAPD分析及其亲缘关系探讨[J].浙江农业学报,2007,19(1):1014.
[8]Fu J Y,Li L,Yuan Z J,et al.Molecular phylogenetic analysis of tea green leafhopper(Empoasca vitis)based on mtDNA CO I sequences[J].Journal of Tea Science,2014,34(6):601608.付建玉,李乐,袁志军,等.基于mtDNA CO I的假眼小绿叶蝉系统发育研究[J].茶叶科学,2014,34(6):601608.
[9]Dai J X,Zhang D Z.Phylogenetic analysis of eight species of Pimeliinae based on CO II and cyt b gene sequences[J].Entomological Knowledge,2008,(4):554558.代金霞,张大治.基于线粒体CO II和cyt b基因序列的8种漠甲系统发育关系[J].昆虫知识,2008,(4):554558.
[10]Qiao F,Chen Z N,Chen Z,et al.Phylogenetic relationships among seven Pieridae genera based on partial sequences of the CO I and cyt b genes[J].Chinese Journal of Applied Entomology,2014,51(1):127138.乔枫,陈振宁,陈志,等.基于线粒体CO I和cyt b基因序列研究粉蝶科七属间的系统发生关系[J].应用昆虫学报,2014,51(1):127138.
[11]Zhou N N.Genetic differentiation of geographical population of tea plantation pseudoeye leafhopper based on mitochondrial CO I and CO II genes[D].China Jiliang University,2014周宁宁.基于线粒体CO I和CO II基因的茶园假眼小绿叶蝉地理种群的遗传分化研究[D].中国计量学院,2014.
[12]Zhang Y,Skolnick J.TMalign:a protein structure alignment algorithm based on the TMscore[J].Nucleic Acids Res,2005,33(7):23022309.
[13]Cosson J F,Hutterer R,Libois R,et al.Phylogeographical footprints of the Strait of Gibraltar and Quaternary climatic fluctuations in the western Mediterranean:A case study with the greater whitetoothed shrew,Crocidura russula(Mammalia:Soricidae)[J].Mol Ecol,2005,14(4):11511162.
[14]Balloux F,LugonMoulin N.The estimation of population differentiation with microsatellite markers[J].Mol Ecol,2002,11(2):155165.
[15]Krebs R A.Combining paternally and maternally inherited mitochondrial DNA for analysis of population structure in mussels[J].Mol Ecol,2004,13(6):17011705.
[16]Zhou N N,Wang M X,Cui L,et al.Genetic variation of Empoasca vitis(G?the)(Hemiptera:Cicadellidae)among different geographical populations based on mtDNA CO I complete sequence[J].Acta Ecologica Sinica,2014,34(23):68796889.周宁宁,王梦馨,崔林,等.基于CO I基因全长序列的假眼小绿叶蝉地理种群遗传分化研究[J].生态学报,2014,34(23):68796889.
[17]Wei F W,Nie Y G,Miao H X,et al.Advancements of the researches on biodiversity loss mechanisms[J].Chinese Science Bulletin,2014,59(6):430437.魏辅文,聂永刚,苗海霞,等.生物多样性丧失机制研究进展[J].科学通报,2014,59(6):430437.
[18]Zhang L J,Shen D R,Sun Y X,et al.Analysis of genetic differentiation among geographic populations of Gynaikothrips ficorum(Marchal)(Thysanoptera:Phlaeothripidae)in Yunnan,southwestern China,based on mtDNA CO I gene sequences[J].Acta Entomologica Sinica,2012,55(2):199207.张利娟,沈登荣,孙跃先,等.基于mtDNA CO I基因序列的云南榕母管蓟马不同地理种群的遗传分化分析[J].昆虫学报,2012,55(2):199207.
[19]Teinlek P,Siripattarapravat K,Tirawattanawanich C.Genetic diversity analysis of Thai indigenous chickens based on complete sequences of mitochondrial DNA Dloop region[J].Asianaustralas J Anim Sci,2018,31(6):804811.
[20]Zheng J C,Sun S L,Yue X R,et al.Phylogeny and evolution of the cholesterol transporter NPC1 in insects[J].J Insect Physiol,2018,107:157166.
[21]Yang X M,Lu Y H.Population genetic structure of Helicoverpa armigera in Ningxia,Inner Mongolia and neighboring areas[J].Chinese Journal of Applied Entomology,2018,55(1):2531.杨现明,陆宴辉.基于线粒体DNA的宁夏、内蒙古及周边地区棉铃虫种群遗传结构[J].应用昆虫学报,2018,55(1):2531.
[22]Vleck L D V,Wright S.Evolution and the genetics of populations.vol.1.genetic and biometric foundations[J].Biometrics,1969,25(3):607.
[23]Fu J N,Han B Y,Xiao Q.Mitochondrial CO I and16sRNA evidence for a single species hypothesis of E.vitis,J.formosana and E.onukii in East Asia[J].PLoS One,2014,9(12):e115259.
[24]Meng Z H,Yang M F,Zhou Y F,et al.Molecular phylogenetic analysis of some species of cicadellinae based on mitochondrial cyt b gene[J].Guizhou Agricultural Sciences,2013,41(7):15.孟泽洪,杨茂发,周玉锋,等.基于cyt b基因序列的大叶蝉亚科部分种类分子系统学分析[J].贵州农业科学,2013,41(7):15.