保定城区2种棺头蟋携带的Wolbachia检测和系统进化
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摘要
利用wsp基因特异引物(81F/691R)对河北保定城区2种棺头蟋——多伊棺头蟋Loxoblemmus doenitzi Stein,1881和石首棺头蟋L.equestris Saussure,1877的头、消化道、后足肌肉和性腺携带的Wolbachia情况进行检测.结果发现,多伊棺头蟋L.doenitzi(26/33)和石首棺头蟋L.equestris(22/22)携带Wolbachia的比例很高,且头和后足肌肉检出率明显高于消化道和性腺.多伊棺头蟋L.doenitzi和石首棺头蟋L.equestris携带的Wolbachia分别属于B组和A组,且各包括2个不同亚组.本研究为进一步研究Wolbachia对棺头蟋生物学的影响奠定了基础.
Wolbachia screening from various tissues of two Loxoblemmus species was performed,including heads,alimentary canal,foot muscles,and gonads.DNA was extracted from these tissues,amplified using Wolbachia surface protein gene(wsp)specific primers(81 F/691 R)followed by sequencing and phylogenetic analysis.All two Loxoblemmus species harbored Wolbachia.The detection ratio of heads and foot muscles apparently higher than alimentary canal and gonads.L.doenitzi and L.equestris presented infection with Wolbachia B and A group,respectively.There were two Wolbachia strains from markedly different subgroup infecting L.doenitzi and L.equestris.These results provide the basis for future study on the effects of Wolbachia on the biology of two Loxoblemmus species.
Wolbachia screening from various tissues of two Loxoblemmus species was performed,including heads,alimentary canal,foot muscles,and gonads.DNA was extracted from these tissues,amplified using Wolbachia surface protein gene(wsp)specific primers(81 F/691 R)followed by sequencing and phylogenetic analysis.All two Loxoblemmus species harbored Wolbachia.The detection ratio of heads and foot muscles apparently higher than alimentary canal and gonads.L.doenitzi and L.equestris presented infection with Wolbachia B and A group,respectively.There were two Wolbachia strains from markedly different subgroup infecting L.doenitzi and L.equestris.These results provide the basis for future study on the effects of Wolbachia on the biology of two Loxoblemmus species.
引文
[1]HILGENBOECKER K,HAMMERSTEIN P,SCHLATTMANN P,et al.How many species are infected with Wolbachia?-a statistical analysis of current data[J].Fems Microbiology Letters,2008,281(2):215-220.DOI:10.1111/j.1574-6968.2008.01110.x.
[2]SONI M,BHATTACHARYA C,SHARMA J,et al.Molecular typing and phylogeny of Wolbachia:A study from Assam,North-Eastern part of India[J].Acta Trop,2017,176:421-426.DOI:10.1016/j.actatropica.2017.09.005.
[3]WERREN J H,BALDO L,CLARK M E.Wolbachia:master manipulators of invertebrate biology[J].Nature Reviews Microbiology,2008,6(10):741-751.DOI:10.1038/nrmicro1969.
[4]ZABAL-AGUIRRE M,ARROYO F,GARCIA-HURTADO J,et al.Wolbachia effects in natural populations of Chorthippus parallelus from the Pyrenean hybrid zone[J].Journal of Evolutionary Biology,2014,27(6):1136-1148.DOI:10.1111/jeb.12389.
[5]ZHANG Y K,DING X L,ZHANG K J,et al.Wolbachia play an important role in affecting mtDNA variation of Tetranychus truncatus(Trombidiformes:Tetranychidae)[J].Environmental Entomology,2013,42(6):1240-1245.DOI:10.1603/EN13085.
[6]MAROJA L S,CLARK M E,HARRISON R G.Wolbachia plays no role in the one-way reproductive incompatibility between the hybridizing field crickets Gryllus firmus and G-pennsylvanicus[J].Heredity,2008,101(5):435-444.DOI:10.1038/hdy.2008.75.
[7]ZHOU W,ROUSSET F,O'NEIL S.Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences[J].Proceedings of the Royal Society B:Biological Sciences,1998,265(1395):509-515.DOI:10.1098/rspb.1998.0324.
[8]JEONG G,AHN J,JANG Y,et al.Wolbachiainfection in the Loxoblemmus complex(Orthoptera:Gryllidae)in Korea[J].Journal of Asia-Pacific Entomology,2012,15(4):563-566.DOI:10.1016/j.aspen.2012.07.002.
[9]陶云荔,郭雅男,王静,等.山东不同地区韭菜迟眼蕈蚊共生菌Wolbachia的检测及鉴定[J].昆虫学报,2015,58(4):454-459.DOI:10.16380/j.kcxb.2015.04.012.TAO Y L,GUO Y N,WANG J,et al.Detection and identification of Wolbachia in Bradysia odoriphaga(Diptera:Sciaridae)populations from Shandong Province,China[J].Acta Entomologica Sinica,2015,58(4):454-459.DOI:10.16380/j.kcxb.2015.04.012.
[10]苗慧,洪晓月,谢霖,等.朱砂叶螨体内感染的Wolbachia的wsp基因序列测定与分析[J].昆虫学报,2004,47(6):738-743.DOI:10.16380/j.kcxb.2004.06.008.MIAO H,HONG X Y,XIE L,et al.Sequencing and sequence analysis of the wsp gene of Wolbachiain Tetranychus cinnabarinus(Acari:Tetranychidae)[J].Acta Entomologica Sinica,2004,47(6):738-743.DOI:10.16380/j.kcxb.2004.06.008.
[11]娄恒,陈雅婷,张艳凯,等.Wolbachia在花蓟马中的感染及其与寄主线粒体DNA多样性的关系[J].昆虫学报,2015,58(7):750-760.DOI:10.16380/j.kcxb.2015.07.007.LOU H,CHEN Y T,ZHANG Y K,et al.Wolbachiainfection and its relationship with mtDNA diversity in the flower thrips,Frankliniella intonsa(Thysanoptera:Thripidae)[J].Acta Entomologica Sinica,2015,58(7):750-760.DOI:10.16380/j.kcxb.2015.07.007.
[12]冯宏祖,王月萍,曹玉,等.苹果蠹蛾体内Wolbachia wsp基因克隆与序列分析[J].应用昆虫学报,2014,51(3):725-732.DOI:10.7679/j.issn.2095-1353.2014.087.FENG H Z,WANG Y P,CAO Y,et al.Cloning and sequence analysis of the Wolbachia wsp gene to detect of Wolbachiain Cydia pomonella[J].Chinese Journal of Applied Entomology,2014,51(3):725-732.DOI:10.7679/j.issn.2095-1353.2014.087.
[13]FOLMER O,BLACK M,HOEH W,et al.DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J].Molecular Marine Biology and Biotechnology,1994,3(5):294-299.
[14]KUMAR S,STECHER G,TAMURA K.MEGA7:molecular evolutionary genetics analysis version 7.0for bigger datasets[J].Molecular Biology and Evolution,2016,33(7):1870-1874.DOI:10.1093/molbev/msw054.
[15]ROZAS J,FERRER-MATA A,SANCHEZ-DELBARRIO J C,et al.DnaSP 6:DNA sequence polymorphism analysis of large data sets[J].Molecular Biology and Evolution,2017,34(12):3299-3302.DOI:10.1093/molbev/msx248.
[16]KAMODA S,MASUI S,ISHIKAWA H,et al.Wolbachiainfection and cytoplasmic incompatibility in the cricket Teleogryllus taiwanemma[J].Journal of Experimental Biology,2000,203(Pt 16):2503-2509.
[17]郭晓鹏,李正西.我国烟粉虱自然种群中存在广泛的Wolbachia感染现象[J].微生物学报,2008,48(1):63-67.DOI:0001-6209(2008)01-0063-05.GUO X P,LI Z X.Wolbachia extensively harbored by Bemisia tabaci in China[J].Acta Microbiologica Sinica,2008,48(1):63-67.DOI:0001-6209(2008)01-0063-05.
[18]SHOEMAKER D D,ROSS K G,KELLER L,et al.Wolbachiainfections in native and introduced populations of fire ants(Solenopsis spp.)[J].Insect Molecular Biology,2000,9(6):661-673.
[2]SONI M,BHATTACHARYA C,SHARMA J,et al.Molecular typing and phylogeny of Wolbachia:A study from Assam,North-Eastern part of India[J].Acta Trop,2017,176:421-426.DOI:10.1016/j.actatropica.2017.09.005.
[3]WERREN J H,BALDO L,CLARK M E.Wolbachia:master manipulators of invertebrate biology[J].Nature Reviews Microbiology,2008,6(10):741-751.DOI:10.1038/nrmicro1969.
[4]ZABAL-AGUIRRE M,ARROYO F,GARCIA-HURTADO J,et al.Wolbachia effects in natural populations of Chorthippus parallelus from the Pyrenean hybrid zone[J].Journal of Evolutionary Biology,2014,27(6):1136-1148.DOI:10.1111/jeb.12389.
[5]ZHANG Y K,DING X L,ZHANG K J,et al.Wolbachia play an important role in affecting mtDNA variation of Tetranychus truncatus(Trombidiformes:Tetranychidae)[J].Environmental Entomology,2013,42(6):1240-1245.DOI:10.1603/EN13085.
[6]MAROJA L S,CLARK M E,HARRISON R G.Wolbachia plays no role in the one-way reproductive incompatibility between the hybridizing field crickets Gryllus firmus and G-pennsylvanicus[J].Heredity,2008,101(5):435-444.DOI:10.1038/hdy.2008.75.
[7]ZHOU W,ROUSSET F,O'NEIL S.Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences[J].Proceedings of the Royal Society B:Biological Sciences,1998,265(1395):509-515.DOI:10.1098/rspb.1998.0324.
[8]JEONG G,AHN J,JANG Y,et al.Wolbachiainfection in the Loxoblemmus complex(Orthoptera:Gryllidae)in Korea[J].Journal of Asia-Pacific Entomology,2012,15(4):563-566.DOI:10.1016/j.aspen.2012.07.002.
[9]陶云荔,郭雅男,王静,等.山东不同地区韭菜迟眼蕈蚊共生菌Wolbachia的检测及鉴定[J].昆虫学报,2015,58(4):454-459.DOI:10.16380/j.kcxb.2015.04.012.TAO Y L,GUO Y N,WANG J,et al.Detection and identification of Wolbachia in Bradysia odoriphaga(Diptera:Sciaridae)populations from Shandong Province,China[J].Acta Entomologica Sinica,2015,58(4):454-459.DOI:10.16380/j.kcxb.2015.04.012.
[10]苗慧,洪晓月,谢霖,等.朱砂叶螨体内感染的Wolbachia的wsp基因序列测定与分析[J].昆虫学报,2004,47(6):738-743.DOI:10.16380/j.kcxb.2004.06.008.MIAO H,HONG X Y,XIE L,et al.Sequencing and sequence analysis of the wsp gene of Wolbachiain Tetranychus cinnabarinus(Acari:Tetranychidae)[J].Acta Entomologica Sinica,2004,47(6):738-743.DOI:10.16380/j.kcxb.2004.06.008.
[11]娄恒,陈雅婷,张艳凯,等.Wolbachia在花蓟马中的感染及其与寄主线粒体DNA多样性的关系[J].昆虫学报,2015,58(7):750-760.DOI:10.16380/j.kcxb.2015.07.007.LOU H,CHEN Y T,ZHANG Y K,et al.Wolbachiainfection and its relationship with mtDNA diversity in the flower thrips,Frankliniella intonsa(Thysanoptera:Thripidae)[J].Acta Entomologica Sinica,2015,58(7):750-760.DOI:10.16380/j.kcxb.2015.07.007.
[12]冯宏祖,王月萍,曹玉,等.苹果蠹蛾体内Wolbachia wsp基因克隆与序列分析[J].应用昆虫学报,2014,51(3):725-732.DOI:10.7679/j.issn.2095-1353.2014.087.FENG H Z,WANG Y P,CAO Y,et al.Cloning and sequence analysis of the Wolbachia wsp gene to detect of Wolbachiain Cydia pomonella[J].Chinese Journal of Applied Entomology,2014,51(3):725-732.DOI:10.7679/j.issn.2095-1353.2014.087.
[13]FOLMER O,BLACK M,HOEH W,et al.DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J].Molecular Marine Biology and Biotechnology,1994,3(5):294-299.
[14]KUMAR S,STECHER G,TAMURA K.MEGA7:molecular evolutionary genetics analysis version 7.0for bigger datasets[J].Molecular Biology and Evolution,2016,33(7):1870-1874.DOI:10.1093/molbev/msw054.
[15]ROZAS J,FERRER-MATA A,SANCHEZ-DELBARRIO J C,et al.DnaSP 6:DNA sequence polymorphism analysis of large data sets[J].Molecular Biology and Evolution,2017,34(12):3299-3302.DOI:10.1093/molbev/msx248.
[16]KAMODA S,MASUI S,ISHIKAWA H,et al.Wolbachiainfection and cytoplasmic incompatibility in the cricket Teleogryllus taiwanemma[J].Journal of Experimental Biology,2000,203(Pt 16):2503-2509.
[17]郭晓鹏,李正西.我国烟粉虱自然种群中存在广泛的Wolbachia感染现象[J].微生物学报,2008,48(1):63-67.DOI:0001-6209(2008)01-0063-05.GUO X P,LI Z X.Wolbachia extensively harbored by Bemisia tabaci in China[J].Acta Microbiologica Sinica,2008,48(1):63-67.DOI:0001-6209(2008)01-0063-05.
[18]SHOEMAKER D D,ROSS K G,KELLER L,et al.Wolbachiainfections in native and introduced populations of fire ants(Solenopsis spp.)[J].Insect Molecular Biology,2000,9(6):661-673.