水稻维生素B_6与SSR标记的关联分析
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摘要
旨在挖掘与维生素B_6(VB_6)相关的SSR标记位点,为水稻高VB_6的分子育种奠定基础。以来源于不同国家和地区的314份水稻品种为材料,对糙米的VB_6进行连续2a的测定,品种VB_6变幅为39.70～138.48μg/g。通过TASSEL3.0软件的GLM和MLM模型对VB_6表型数据与154个SSR标记位点进行关联分析,共检测出13个与VB_6相关联的位点,其中,RM254、RM309和RM540在2a的2种模型中均被检测出,是水稻VB_6的重要SSR位点。同时进一步挖掘出8个优异等位变异及相应的载体材料,发现‘辽星6’‘越实’‘花脸’等有较高VB_6,且至少含有2个以上优异等位变异。
This study is designed to excavate the SSR markers associated with vitamin B_6(VB_6),and provide a foundation for molecular breeding of high VB_6 in rice.314 rice varieties from different countries and regions were selected as materials,and VB_6 mass fraction in brown rice were determined in two consecutive years.The results showed that the VB_6 mass fraction in the varieties ranged from39.70μg/g to 138.48μg/g.A total of 13 sites were detected through association mapping of 154 SSR markers with VB_6 mass fraction in GLM and MLM models of TASSEL3.0.Among them,RM254,RM309,and RM540 were detected in two years' data with two models.Eight excellent alleles and corresponding carrier materials were further identified.Excellent varieties with higher VB_6 mass fraction,such as‘Liaoxing 6',‘Yueshi',‘Hualian',etc.,contain 2more excellent alleles.
This study is designed to excavate the SSR markers associated with vitamin B_6(VB_6),and provide a foundation for molecular breeding of high VB_6 in rice.314 rice varieties from different countries and regions were selected as materials,and VB_6 mass fraction in brown rice were determined in two consecutive years.The results showed that the VB_6 mass fraction in the varieties ranged from39.70μg/g to 138.48μg/g.A total of 13 sites were detected through association mapping of 154 SSR markers with VB_6 mass fraction in GLM and MLM models of TASSEL3.0.Among them,RM254,RM309,and RM540 were detected in two years' data with two models.Eight excellent alleles and corresponding carrier materials were further identified.Excellent varieties with higher VB_6 mass fraction,such as‘Liaoxing 6',‘Yueshi',‘Hualian',etc.,contain 2more excellent alleles.
引文
[1]FITZGERALD M A,MCCOUCH S R,HALL R D.Not just a grain of rice:the quest for quality[J].Trends in Plant Science,2009,14(3):133-139.
[2]江良荣,李义珍,王侯聪,等.稻米营养品质的研究现状及分子改良途径[J].分子植物育种,2004,2(1):113-121.JIANG L R,LI Y ZH,WANG H C,et al.Research status of rice nutrition quality and molecular improvement approach[J].Molecular Plant Breeding,2004,2(1):113-121.
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[4]GALLUZZI L,VACCHELLI E,MICHELS J,et al.Effects of vitamin B6 metabolism on oncogenesis,tumor progression and therapeutic responses[J].Oncogene,2013,32(42):4995-5004.
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[6]KIM,YOUNG-NAM,YOUN-OK CHO.Dietary intake and major food sources of vitamin B6in Korean adults[J].The FASEB Journal,2013,27(1):1077-1086.
[7]EHRENSHAFT M,BILSKI P,LI M Y,et al.A highly conserved sequence is a novel gene involved in de novo vitamin B6biosynthesis[J].Proceedings of the National Academy of Sciences of the United States of America,1999,96(16):9374.
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[10]刘海峰.AvrRxo1调控水稻维生素B6合成影响水稻对条斑病抗性[D].山东泰安:山东农业大学,2015.LIU H F.AvrRxo1regulates rice resistant to bacterial leaf streak by interfering the synthesis of vitamin B6[D].Tai’an Shandong:Shandong Agricultural University,2015.
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[12]金亮.水稻关联定位群体的构建及若干品质性状的关联分析[D].杭州:浙江大学,2009.JIN L.Construetion of an assoeiation population and association analysis for some quality trait in rice[D].Hangzhou:Zhejiang University,2009.
[13]文自翔,赵团结,郑永战,等.中国栽培和野生大豆农艺品质性状与SSR标记的关联分析Ⅰ.群体结构及关联标记[J].作物学报,2008,34(7):1169-1178.WEN Z X,ZHAO T J,ZHENG Y ZH,et al.Association analysis of agronomic and quality traits with SSR markers in glycine max and glycine soja in ChinaⅠ.Population structure and associated markers[J].Acta Agronomica Sinica,2008,34(7):1169-1178.
[14]武玉国,吴承来,秦保平,等.黄淮冬麦区175个小麦品种的遗传多样性及SSR标记与株高和产量相关性状的关联分析[J].作物学报,2012,38(6):1018-1028.WU Y G,WU CH L,QIN B P,et al.Diversity of 175wheat varieties from yellow and huai river valleys facultative wheat zone and association of SSR markers with plant height and yield related traits[J].Acta Agronomica Sinica,2012,38(6):1018-1028.
[15]DOYLE J.Isolation of plant DNA from fresh tissue[J].Focus,1991,12:13-15.
[16]EVANNO G,REGNAUT S,GOUDET J.Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study[J].Molecular Ecology,2005,14(8):2611-2620.
[17]BRESEGHELLO F,SORRELLS M E.Association mapping of kernel size and milling quality in wheat(Triticum aestivum L.)cultivars.[J].Genetics,2006,172(2):1165.
[18]LI X,YAN W,AGRAMA H,et al.Mapping QTLs for improving grain yield using the USDA rice mini-core collection[J].Planta,2011,234(2):347-361.
[19]BRADBURY P J,ZHANG Z,KROON D E,et al.TAS-SEL:software for association mapping of complex traits in diverse samples.[J].Bioinformatics,2007,23(19):2633-2635.
[20]ZHANG T,QIAN N,ZHU X,et al.Variations and transmission of QTL alleles for yield and fiber qualities in upland cotton cultivars developed in China[J].Plos One,2013,8(2):e57220.
[21]杨小红,严建兵,郑艳萍,等.植物数量性状关联分析研究进展[J].作物学报,2007,33(4):523-530.YANG X H,YAN J B,ZHENG Y P,et al.Reviews of association analysis for quantitative traits in plants[J].Acta Agronomica Sinica,2007,33(4):523-530.
[22]KLINE J B,MOORE D J,CLEVENGER C V.Activation and association of the Tec tyrosine kinase with the human prolactin receptor:mapping of a Tec/Vav1-receptor binding site[J].Molecular Endocrinology,2001,15(5):832-41.
[23]HARRIS B P,STOKESBURY K D E.The spatial structure of local surficial sediment characteristics on Georges Bank,USA[J].Continental Shelf Research,2010,30(17):1840-1853.
[2]江良荣,李义珍,王侯聪,等.稻米营养品质的研究现状及分子改良途径[J].分子植物育种,2004,2(1):113-121.JIANG L R,LI Y ZH,WANG H C,et al.Research status of rice nutrition quality and molecular improvement approach[J].Molecular Plant Breeding,2004,2(1):113-121.
[3]HELLMANN H,MOONEY S.Vitamin B6:a molecule for human health?[J].Molecules,2010,15(1):442-59.
[4]GALLUZZI L,VACCHELLI E,MICHELS J,et al.Effects of vitamin B6 metabolism on oncogenesis,tumor progression and therapeutic responses[J].Oncogene,2013,32(42):4995-5004.
[5]DI S M,SAFO M K,CONTESTABILE R.Biomedical aspects of pyridoxal 5′-phosphate availability[J].Frontiers in Bioscience,2012,4(3):897.
[6]KIM,YOUNG-NAM,YOUN-OK CHO.Dietary intake and major food sources of vitamin B6in Korean adults[J].The FASEB Journal,2013,27(1):1077-1086.
[7]EHRENSHAFT M,BILSKI P,LI M Y,et al.A highly conserved sequence is a novel gene involved in de novo vitamin B6biosynthesis[J].Proceedings of the National Academy of Sciences of the United States of America,1999,96(16):9374.
[8]EHRENSHAFT M,DAUB M E.Isolation of PDX2,a second novel gene in the pyridoxine biosynthesis pathway of eukaryotes,archaebacteria,and a subset of eubacteria[J].Journal of Bacteriology,2001,183(11):3383-3390.
[9]TAMBASCO-STUDART M,TITIZ O,RASCHLE T,et al.Vitamin B6biosynthesis in higher plants[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(38):13687.
[10]刘海峰.AvrRxo1调控水稻维生素B6合成影响水稻对条斑病抗性[D].山东泰安:山东农业大学,2015.LIU H F.AvrRxo1regulates rice resistant to bacterial leaf streak by interfering the synthesis of vitamin B6[D].Tai’an Shandong:Shandong Agricultural University,2015.
[11]FLINT-GARCIA S A,THORNSBERRY J M.Structure of linkage disequilibrium in plants[J].Annual Review of Plant Biology,2003,54(4):357.
[12]金亮.水稻关联定位群体的构建及若干品质性状的关联分析[D].杭州:浙江大学,2009.JIN L.Construetion of an assoeiation population and association analysis for some quality trait in rice[D].Hangzhou:Zhejiang University,2009.
[13]文自翔,赵团结,郑永战,等.中国栽培和野生大豆农艺品质性状与SSR标记的关联分析Ⅰ.群体结构及关联标记[J].作物学报,2008,34(7):1169-1178.WEN Z X,ZHAO T J,ZHENG Y ZH,et al.Association analysis of agronomic and quality traits with SSR markers in glycine max and glycine soja in ChinaⅠ.Population structure and associated markers[J].Acta Agronomica Sinica,2008,34(7):1169-1178.
[14]武玉国,吴承来,秦保平,等.黄淮冬麦区175个小麦品种的遗传多样性及SSR标记与株高和产量相关性状的关联分析[J].作物学报,2012,38(6):1018-1028.WU Y G,WU CH L,QIN B P,et al.Diversity of 175wheat varieties from yellow and huai river valleys facultative wheat zone and association of SSR markers with plant height and yield related traits[J].Acta Agronomica Sinica,2012,38(6):1018-1028.
[15]DOYLE J.Isolation of plant DNA from fresh tissue[J].Focus,1991,12:13-15.
[16]EVANNO G,REGNAUT S,GOUDET J.Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study[J].Molecular Ecology,2005,14(8):2611-2620.
[17]BRESEGHELLO F,SORRELLS M E.Association mapping of kernel size and milling quality in wheat(Triticum aestivum L.)cultivars.[J].Genetics,2006,172(2):1165.
[18]LI X,YAN W,AGRAMA H,et al.Mapping QTLs for improving grain yield using the USDA rice mini-core collection[J].Planta,2011,234(2):347-361.
[19]BRADBURY P J,ZHANG Z,KROON D E,et al.TAS-SEL:software for association mapping of complex traits in diverse samples.[J].Bioinformatics,2007,23(19):2633-2635.
[20]ZHANG T,QIAN N,ZHU X,et al.Variations and transmission of QTL alleles for yield and fiber qualities in upland cotton cultivars developed in China[J].Plos One,2013,8(2):e57220.
[21]杨小红,严建兵,郑艳萍,等.植物数量性状关联分析研究进展[J].作物学报,2007,33(4):523-530.YANG X H,YAN J B,ZHENG Y P,et al.Reviews of association analysis for quantitative traits in plants[J].Acta Agronomica Sinica,2007,33(4):523-530.
[22]KLINE J B,MOORE D J,CLEVENGER C V.Activation and association of the Tec tyrosine kinase with the human prolactin receptor:mapping of a Tec/Vav1-receptor binding site[J].Molecular Endocrinology,2001,15(5):832-41.
[23]HARRIS B P,STOKESBURY K D E.The spatial structure of local surficial sediment characteristics on Georges Bank,USA[J].Continental Shelf Research,2010,30(17):1840-1853.