Developing green super rice varieties with high nutrient use efficiency by phenotypic selection under varied nutrient conditions
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摘要
The development of green super rice varieties with improved nutrient use efficiency(NuUE)is a vital target area to increase yield and make it more stable under rainfed conditions.In the present study, we followed an early backcross(BC) breeding approach by using a highyielding and widely adapted Xian variety, Weed Tolerant Rice 1(WTR-1), as a recipient and a Geng variety, Hao-An-Nong(HAN), as a donor.Starting from the BC_1F_2 generation, the BC population went through one generation of selection under irrigated, low-input, and rainfed conditions, followed by four consecutive generations of screening and selection for high grain yield(GY) under six different nutrient conditions(NPK, 75 N,-N,-P,-NP, and-NPK), leading to the development of 230 BC_1F_6 introgression lines(ILs).These 230 ILs were evaluated under the same six nutrient conditions for 13 agro-morphological and grain yield component traits in comparison to four checks and parents.Significant trait variations were observed between the treatments and ILs.Positive correlations were identified for GY with biomass, panicle length, flag-leaf area, flag-leaf width, filled grain number per panicle,1000-grain weight, and tiller number under-N,-P,-NP, and-NPK conditions.Out of 230 ILs,12 were identified as promising under two or more nutrient deficiency conditions.The results demonstrated an efficient inter-subspecific BC breeding procedure with a first round of selection under rainfed-drought conditions, followed by four generations of progeny testing for yield performance under six nutrient conditions.The promising ILs can be useful resources for molecular genetic dissection and understanding the physiological mechanisms of NuUE.
The development of green super rice varieties with improved nutrient use efficiency(NuUE)is a vital target area to increase yield and make it more stable under rainfed conditions.In the present study, we followed an early backcross(BC) breeding approach by using a highyielding and widely adapted Xian variety, Weed Tolerant Rice 1(WTR-1), as a recipient and a Geng variety, Hao-An-Nong(HAN), as a donor.Starting from the BC_1F_2 generation, the BC population went through one generation of selection under irrigated, low-input, and rainfed conditions, followed by four consecutive generations of screening and selection for high grain yield(GY) under six different nutrient conditions(NPK, 75 N,-N,-P,-NP, and-NPK), leading to the development of 230 BC_1F_6 introgression lines(ILs).These 230 ILs were evaluated under the same six nutrient conditions for 13 agro-morphological and grain yield component traits in comparison to four checks and parents.Significant trait variations were observed between the treatments and ILs.Positive correlations were identified for GY with biomass, panicle length, flag-leaf area, flag-leaf width, filled grain number per panicle,1000-grain weight, and tiller number under-N,-P,-NP, and-NPK conditions.Out of 230 ILs,12 were identified as promising under two or more nutrient deficiency conditions.The results demonstrated an efficient inter-subspecific BC breeding procedure with a first round of selection under rainfed-drought conditions, followed by four generations of progeny testing for yield performance under six nutrient conditions.The promising ILs can be useful resources for molecular genetic dissection and understanding the physiological mechanisms of NuUE.
The development of green super rice varieties with improved nutrient use efficiency(NuUE)is a vital target area to increase yield and make it more stable under rainfed conditions.In the present study, we followed an early backcross(BC) breeding approach by using a highyielding and widely adapted Xian variety, Weed Tolerant Rice 1(WTR-1), as a recipient and a Geng variety, Hao-An-Nong(HAN), as a donor.Starting from the BC_1F_2 generation, the BC population went through one generation of selection under irrigated, low-input, and rainfed conditions, followed by four consecutive generations of screening and selection for high grain yield(GY) under six different nutrient conditions(NPK, 75 N,-N,-P,-NP, and-NPK), leading to the development of 230 BC_1F_6 introgression lines(ILs).These 230 ILs were evaluated under the same six nutrient conditions for 13 agro-morphological and grain yield component traits in comparison to four checks and parents.Significant trait variations were observed between the treatments and ILs.Positive correlations were identified for GY with biomass, panicle length, flag-leaf area, flag-leaf width, filled grain number per panicle,1000-grain weight, and tiller number under-N,-P,-NP, and-NPK conditions.Out of 230 ILs,12 were identified as promising under two or more nutrient deficiency conditions.The results demonstrated an efficient inter-subspecific BC breeding procedure with a first round of selection under rainfed-drought conditions, followed by four generations of progeny testing for yield performance under six nutrient conditions.The promising ILs can be useful resources for molecular genetic dissection and understanding the physiological mechanisms of NuUE.
引文
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[23]Y.Aluwihare,M.Ishan,M.Chamikara,C.Weebadde,D.Sirisena,W.Samarasinghe,S.Sooriyapathirana,Characterization and selection of phosphorus deficiency tolerant rice genotypes in Sri Lanka,Rice Sci.23(2016)184-195.
[24]A.Mahender,A.Anandan,S.K.Pradhan,O.N.Singh,Traitsrelated QTLs and genes and their potential applications in rice improvement under low phosphorus condition,Arch.Agron.Soil Sci.15(2017)1-16.
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[26]Y.He,T.Zheng,X.Hao,L.Wang,Y.Gao,Z.Hua,H.Q.Zhai,J.L.Xu,Z.J.Xu,L.H.Zhu,Z.K.Li,Yield performances of japonica introgression lines selected for drought tolerance in a BCbreeding programme,Plant Breed.129(2010)167-175.
[27]Y.Cui,W.Zhang,X.Lin,S.Xu,J.Xu,Z.Li,Simultaneous improvement and genetic dissection of drought tolerance using selected breeding populations of rice,Front.Plant Sci.(2018)320.
[28]Z.Li,B.Fu,Y.Gao,J.Xu,J.Ali,H.Lafitte,Y.Z.Jiang,J.D.Rey,C.H.Vijayakumar,R.Maghirang,T.Q.Zheng,L.H.Zhu,Genome wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice(Oryza sativa L.),Plant Mol.Biol.59(2005)33-52.
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[32]I.S.El-Refaee,R.A.Ebaid,I.M.El-Rewiny,Performance of rice(Oryza sativa L.)plant under different water regimes and methods of planting,Alex.J.Agric.Res.51(2006)47-55.
[33]M.A.Hossaen,A.T.M.Shamsuddoha,A.K.Paul,M.S.I.Bhuiyan,A.S.M.Zobaer,Efficacy of different organic manures and inorganic fertilizer on the yield and yield attributes of Boro rice,Agriculturists 9(2011)117-125.
[34]A.S.Iqbal,M.K.Abbasi,G.Rasool,Integrated plant nutrition system(IPNS)in wheat under rainfed condition of Rawalkot Azad Jammu and Kashmir,Pak.J.Soil.Sci.21(2002)79-86.
[35]J.Ju,Y.Yamamoto,Y.L.Wang,Y.H.Shan,G.C.Dong,T.Yoshida,A.Miyazaki,Genotypic differences in grain yield,and nitrogen absorption and utilization in recombinant inbred lines of rice under hydroponic culture,Soil Sci.Plant Nutr.52(2006)321-330.
[36]H.Tong,H.L.Chen,W.P.Li,H.W.Mei,Y.Z.Xing,X.Q.Yu,X.Y.Xu,S.Q.Zhang,L.J.Luo,Identification and characterization of quantitative trait loci for grain yield and its components under different nitrogen fertilization levels in rice(Oryza sativa L.),Mol.Breed.28(2011)495-509.
[37]A.Makino,Photosynthesis,grain yield,and nitrogen utilization in rice and wheat,Plant Physiol.155(2011)125-129.
[38]S.Y.Tabar,Effect of nitrogen and phosphorus fertilizer on growth and yield rice(Oryza sativa L.),Int.J.Agron.Plant Prod.3(2012)579-584.
[39]Inamullah Amanullah,Dry matter partitioning and harvest index differ in rice genotypes with variable rates of phosphorus and zinc nutrition,Rice Sci.23(2016)78-87.
[40]X.Lin,Q.W.J.Zhou,D.F.Zhu,Y.P.Zhang,Effect of SWDirrigation on photosynthesis and grain yield of rice(Oryza sativa L.),Field Crops Res.94(2005)67-75.
[41]I.Chaturvedi,Effect of nitrogen fertilizers on growth,yield and quality of hybrid rice(Oryza sativa L.),J.Central Eur.Agric.6(2005)611-618.
[42]N.N.Mandal,P.P.Chaudhry,D.Sinha,Nitrogen,phosphorus and potash uptake of wheat(var.Sonalika),Environ.Ecol.10(1992)297.
[43]S.Singh,L.C.Ram,Effect of fertilizer and organic manure on root CEC of some rice and wheat varieties,J.Indian Soc.Soil Sci.24(1976)427-431.
[44]S.Yoshida,J.H.Cock,F.T.Parao,Physiological aspects of grain yield,Annu.Rev.Plant Physiol.23(1972)437-464.
[2]P.L.Pingali,Green revolution:impacts,limits,and the path ahead,Proc.Natl.Acad.Sci.U.S.A.109(2012)12302-12308.
[3]Q.Zhang,Strategies for developing green super rice,Proc.Natl.Acad.Sci.U.S.A.104(2007)16402-16409.
[4]K.G.Cassman,S.Peng,D.C.Olk,J.K.Ladha,W.Reichardt,A.Dobermann,U.Singh,Opportunities for increased nitrogenuse efficiency from improved resource management in irrigated rice systems,Field Crops Res.56(1998)7-39.
[5]S.Senthilvel,P.Govindaraj,S.Arumugachamy,R.Latha,P.Malarvizhi,A.Gopalan,M.Maheswaran,Mapping genetic loci associated with nitrogen use efficiency in rice(Oryza sativa L.),Proceedings of the 4th International Crop Science Congress,Sept.26-Oct.1,Brisbane,Australia 2004,p.2004.
[6]J.K.Ladha,H.Pathak,T.J.Krupnik,J.Six,C.van Kessel,Efficiency of fertilizer nitrogen in cereal production:retrospects and prospects,Adv.Agron.87(2005)85-156.
[7]T.L.Roberts,Improving nutrient use efficiency,Turk.J.Agric.For.32(2008)177-182.
[8]N.K.Fageria,V.C.Baligar,Enhancing nitrogen use efficiency in crop plants,Adv.Agron.88(2005)97-185.
[9]M.K.Papademetriou,Rice production in the Asia-Pacific region:issues and perspectives,Report of the Expert Consultation on Bridging the Rice Yield Gap in the Asia-Pacific Region,FAO Regional Office for Asia and the Pacific,Bangkok,Thailand,1999.
[10]J.Ali,J.L.Xu,Y.M.Gao,X.F.Ma,L.J.Meng,Y.Wang,Y.L.Pang,Y.S.Guan,M.R.Xu,J.E.Revilleza,N.J.Franje,S.C.Zhou,Z.K.Li,Harnessing the hidden genetic diversity for improving multiple abiotic stress tolerance in rice(Oryza sativa L.),PLoSOne 12(2017),e0172515.
[11]B.Sarkar,R.Naidu,Nutrient and water use efficiency in soil:the influence of geological mineral amendments,in:A.Rakshit,B.H.Singh,A.Sen(Eds.),Nutrient Use Efficiency:From Basics to Advances,Springer,New Delhi,India 2015,pp.29-44.
[12]K.K.Vinod,S.Heuer,Approaches towards nitrogen-and phosphorus-efficient rice,AoB Plants(2012)pls028.
[13]V.C.Baligar,N.K.Fageria,Z.L.He,Nutrient use efficiency in plants,Commun.Soil Sci.Plant Anal.32(2001)921-950.
[14]C.Van Hach,N.Nam,Responses of some promising highyielding rice varieties to nitrogen fertilizer,Omonrice 14(2006)78-91.
[15]K.Saito,B.Linquist,G.N.Atlin,K.Phanthaboon,T.Shiraiwa,T.Horie,Response of traditional and improved upland rice cultivars to N and P fertilizer in northern Laos,Field Crops Res.96(2006)216-223.
[16]M.Alam,M.Ali,A.Amin,M.Hasanuzzaman,Yield attributes,yield and harvest index of three irrigated rice varieties under different levels of phosphorus,Adv.Biol.Res.3(2009)132-139.
[17]A.A.B.Abou-Khalifa,Evaluation of some rice varieties under different nitrogen levels,Adv.Appl.Sci.Res.3(2012)1144-1149.
[18]R.Sharma,R.K.Gangwar,V.Yadav,R.Kumar,Response of basmati rice(Oryza sativa)cultivars to graded nitrogen levels under transplanted condition,IMPACT:Int.J.Res.Appl.Nat.Social Sci.2(2014)33-38.
[19]W.Wang,R.Mauleon,Z.Hu,D.Chebotarov,S.Tai,Z.Wu,M.Li,T.Zheng,R.R.Fuentes,F.Zhang,L.Mansueto,D.Copetti,M.Sanciangco,K.C.Palis,J.Xu,C.Sun,B.Fu,H.Zhang,Y.Gao,X.Zhao,F.Shen,X.Cui,H.Yu,Z.Li,M.Chen,J.Detras,Y.Zhou,X.Zhang,Y.Zhao,D.Kudrna,C.Wang,R.Li,B.Jia,J.Lu,X.He,Z.Dong,J.Xu,Y.Li,M.Wang,J.Shi,J.Li,D.Zhang,S.Lee,W.Hu,A.Poliakov,I.Dubchak,V.J.Ulat,F.N.Borja,J.R.Mendoza,J.Ali,J.Li,Q.Gao,Y.Niu,Z.Yue,M.E.B.Naredo,J.Talag,X.Wang,J.Li,X.Fang,Y.Yin,J.-C.Glaszmann,J.Zhang,J.Li,R.Sackville Hamilton,R.A.Wing,J.Ruan,G.Zhang,C.Wei,N.Alexandrov,K.L.McNally,Z.Li,H.Leung,Genomic variation in 3,010 diverse accessions of Asian cultivated rice,Nature(2018)43-49.
[20]R.Core Team,R:A Language and Environment for Statistical Computing,R Foundation for Statistical Computing,Vienna,Austria,2013,URL http://www.R-project.org/.
[21]M.Panigrahy,D.N.Rao,P.Yugandhar,N.S.Raju,P.Krishnamurthya,S.R.Voletia,G.A.Reddy,T.Mohapatra,S.Robin,A.K.Singh,K.Singh,M.Sheshshayeef,R.P.Sharma,N.Sarla,Hydroponic experiment for identification of tolerance traits developed by rice Nagina 22 mutants to lowphosphorus in field condition,Arch.Agron.Soil Sci.60(2014)565-576.
[22]N.K.Fageria,J.P.Oliveira,Nitrogen,phosphorus and potassium interactions in upland rice,J.Plant Nutr.37(2014)1586-1600.
[23]Y.Aluwihare,M.Ishan,M.Chamikara,C.Weebadde,D.Sirisena,W.Samarasinghe,S.Sooriyapathirana,Characterization and selection of phosphorus deficiency tolerant rice genotypes in Sri Lanka,Rice Sci.23(2016)184-195.
[24]A.Mahender,A.Anandan,S.K.Pradhan,O.N.Singh,Traitsrelated QTLs and genes and their potential applications in rice improvement under low phosphorus condition,Arch.Agron.Soil Sci.15(2017)1-16.
[25]A.J.Ali,J.L.Xu,A.M.Ismail,B.Y.Fu,C.H.M.Vijaykumar,Y.M.Gao,J.Domingo,R.Maghirang,S.B.Yu,G.Gregorio,S.Yanaghihara,M.Cohen,B.Carmen,D.Mackill,Z.K.Li,Hidden diversity for abiotic and biotic stress tolerances in the primary gene pool of rice revealed by a large backcross breeding program,Field Crops Res.(2006)66-76.
[26]Y.He,T.Zheng,X.Hao,L.Wang,Y.Gao,Z.Hua,H.Q.Zhai,J.L.Xu,Z.J.Xu,L.H.Zhu,Z.K.Li,Yield performances of japonica introgression lines selected for drought tolerance in a BCbreeding programme,Plant Breed.129(2010)167-175.
[27]Y.Cui,W.Zhang,X.Lin,S.Xu,J.Xu,Z.Li,Simultaneous improvement and genetic dissection of drought tolerance using selected breeding populations of rice,Front.Plant Sci.(2018)320.
[28]Z.Li,B.Fu,Y.Gao,J.Xu,J.Ali,H.Lafitte,Y.Z.Jiang,J.D.Rey,C.H.Vijayakumar,R.Maghirang,T.Q.Zheng,L.H.Zhu,Genome wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice(Oryza sativa L.),Plant Mol.Biol.59(2005)33-52.
[29]J.Ali,J.Xu,Y.Gao,M.Fontanilla,Z.Li,Breeding for yield potential and enhanced productivity across different rice ecologies through green super rice(GSR)breeding strategy,in:K.Muralidharan,E.A.Siddiq(Eds.),International Dialogue on Perception and Prospects of Designer Rice,Society for the Advancement of Rice Research,Directorate of Rice Research,Hyderabad,India 2013,pp.60-68.
[30]N.K.Fageria,V.C.Baligar,A.B.Heinemann,M.C.S.Carvalho,Nitrogen uptake and use efficiency in rice,in:A.Rakshit,B.H.Singh,A.Sen(Eds.),Nutrient Use Efficiency:From Basics to Advances,Springer,New Delhi,India 2015,pp.285-296.
[31]R.M.Ahmad,M.Naveed,Z.A.Aslam,M.Arshad,Economizing the use of nitrogen fertilizer in wheat production through enriched compost,Renew.Agric.Food Syst.23(2008)243-249.
[32]I.S.El-Refaee,R.A.Ebaid,I.M.El-Rewiny,Performance of rice(Oryza sativa L.)plant under different water regimes and methods of planting,Alex.J.Agric.Res.51(2006)47-55.
[33]M.A.Hossaen,A.T.M.Shamsuddoha,A.K.Paul,M.S.I.Bhuiyan,A.S.M.Zobaer,Efficacy of different organic manures and inorganic fertilizer on the yield and yield attributes of Boro rice,Agriculturists 9(2011)117-125.
[34]A.S.Iqbal,M.K.Abbasi,G.Rasool,Integrated plant nutrition system(IPNS)in wheat under rainfed condition of Rawalkot Azad Jammu and Kashmir,Pak.J.Soil.Sci.21(2002)79-86.
[35]J.Ju,Y.Yamamoto,Y.L.Wang,Y.H.Shan,G.C.Dong,T.Yoshida,A.Miyazaki,Genotypic differences in grain yield,and nitrogen absorption and utilization in recombinant inbred lines of rice under hydroponic culture,Soil Sci.Plant Nutr.52(2006)321-330.
[36]H.Tong,H.L.Chen,W.P.Li,H.W.Mei,Y.Z.Xing,X.Q.Yu,X.Y.Xu,S.Q.Zhang,L.J.Luo,Identification and characterization of quantitative trait loci for grain yield and its components under different nitrogen fertilization levels in rice(Oryza sativa L.),Mol.Breed.28(2011)495-509.
[37]A.Makino,Photosynthesis,grain yield,and nitrogen utilization in rice and wheat,Plant Physiol.155(2011)125-129.
[38]S.Y.Tabar,Effect of nitrogen and phosphorus fertilizer on growth and yield rice(Oryza sativa L.),Int.J.Agron.Plant Prod.3(2012)579-584.
[39]Inamullah Amanullah,Dry matter partitioning and harvest index differ in rice genotypes with variable rates of phosphorus and zinc nutrition,Rice Sci.23(2016)78-87.
[40]X.Lin,Q.W.J.Zhou,D.F.Zhu,Y.P.Zhang,Effect of SWDirrigation on photosynthesis and grain yield of rice(Oryza sativa L.),Field Crops Res.94(2005)67-75.
[41]I.Chaturvedi,Effect of nitrogen fertilizers on growth,yield and quality of hybrid rice(Oryza sativa L.),J.Central Eur.Agric.6(2005)611-618.
[42]N.N.Mandal,P.P.Chaudhry,D.Sinha,Nitrogen,phosphorus and potash uptake of wheat(var.Sonalika),Environ.Ecol.10(1992)297.
[43]S.Singh,L.C.Ram,Effect of fertilizer and organic manure on root CEC of some rice and wheat varieties,J.Indian Soc.Soil Sci.24(1976)427-431.
[44]S.Yoshida,J.H.Cock,F.T.Parao,Physiological aspects of grain yield,Annu.Rev.Plant Physiol.23(1972)437-464.