紫外串联荧光检测法测定奶昔中维生素B_6和烟酰胺
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摘要
目的建立紫外串联荧光检测法同时快速检测奶昔中维生素B_6和烟酰胺的分析方法。方法参照GB 5009.154-2016《食品中维生素B_6的测定》和GB 5009.89-2016《食品中烟酸和烟酰胺的测定》,采用带有紫外检测器和荧光检测器的高效液相色谱仪快速检测奶昔中维生素B_6和烟酰胺。结果该方法的准确度偏差<15%,线性相关系数>0.99,重复性和稳定性均符合标准要求,平均回收率在95%～105%之间,均符合标准GB/T 27404-2008《实验室质量控制规范食品理化检测》中的要求,且目标峰分离效果更好,效率提升49.5%。结论该方法的检测效率和分离效果较好,符合标准要求。
Objective To establish a method for rapid and simultaneous determination of vitamin B_6 and nicotinamide in milk shake by ultraviolet tandem fluorescence method. Methods According to GB 5009.154-2016 Determination of vitamin B_6 in foods and GB 5009.89-2016 Determination of niacin and nicotinamide in foods,vitamin B_6 and nicotinamide in milk shake was determined by high performance liquid chromatography with UV detector and fluorescence detector. Results The accuracy deviation of this method was less than 15%, and the linear correlation coefficient was more than 0.99. The repeatability and stability were in line with the standard requirements,and the average recovery rate was between 95%-105%, which met the requirements of the standard GB/T 27404-2008 Laboratory quality control standard for food physical and chemical testing. The separation of target peaks was better,and the efficiency was increased by 49.5%. Conclusion The established method has better efficiency and separation effect, which can meet the standard requirements.
Objective To establish a method for rapid and simultaneous determination of vitamin B_6 and nicotinamide in milk shake by ultraviolet tandem fluorescence method. Methods According to GB 5009.154-2016 Determination of vitamin B_6 in foods and GB 5009.89-2016 Determination of niacin and nicotinamide in foods,vitamin B_6 and nicotinamide in milk shake was determined by high performance liquid chromatography with UV detector and fluorescence detector. Results The accuracy deviation of this method was less than 15%, and the linear correlation coefficient was more than 0.99. The repeatability and stability were in line with the standard requirements,and the average recovery rate was between 95%-105%, which met the requirements of the standard GB/T 27404-2008 Laboratory quality control standard for food physical and chemical testing. The separation of target peaks was better,and the efficiency was increased by 49.5%. Conclusion The established method has better efficiency and separation effect, which can meet the standard requirements.
引文
[1]黄绿苗.维生素B6的进展性研究[J].医学美学美容(中旬刊),2014,(7):607.Huang LM.Progressive study of vitamin B6[J].Med Aesthetics Beauty(Middle),2014,(7):607.
[2]沈丽平.HPLC测定维生素B6片含量测定方法的探讨[J].海峡药学,2016,28(2):75-76.Shen LP.Discussion on the determination method of vitamin B6 tablets by HPLC[J].Strait Pharm J,2016,28(2):75-76.
[3]曹高娃,崔毅,张力,等.紫外分光光度法测定维生素B6[J].光谱实验室,2001,18(6):802-803.Cao GW,Cui Y,Zhang L,et al.Determination of vitamin B6 by ultraviolet spectrophotometry[J].Spectrosc Lab,2001,18(6):802-803.
[4]Galluzzi L,Kepp O,Vander-Heiden MG,et al.Metabolic targets for cancer therapy[J].Nat Rev Drug Discov,2013,12(11):829-846.
[5]Vora M,Ansari J,Shanti RM,et al.Increased nicotinamide phosphoribosyltransferase in rhabdomyosarcomas and leiomyosarcomas compared to skeletal and smooth muscle tissue[J].Anticancer Res,2016,36(2):503-507.
[6]韩永利,李秋凤,李建国.烟酸对奶牛的营养作用[J].饲料博览,2002,(3):33-34.Han YL,Li QF,Li JG.Nutrition effect of niacin on dairy cows[J].Feed Expo,2002,(3):33-34.
[7]GB 5009.154-2016食品中维生素B6的测定[S].GB 5009.154-2016 Determination of vitamin B6 in food[S].
[8]GB 14753-2010食品添加剂中维生素B6的测定[S].GB 14753-2010 Determination of vitamin B6 in food additives[S]
[9]GB 7298-2017饲料添加剂中维生素B6的测定[S].GB 7298-2017 Determination of vitamin B6 in feed additives[S].
[10]GB/T 33309-2016化妆品中维生素B6的测定[S].GB/T 33309-2016 Determination of vitamin B6 in cosmetics[S].
[11]SN/T 0549-1996出口蜂王浆及干粉中维生素B6的测定[S].SN/T 0549-1996 Determination of vitamin B6 in exported royal jelly and dry powder[S].
[12]GB 5009.89-2016食品中烟酸和烟酰胺的测定[S].GB 5009.89-2016 Determination of niacin and nicotinamide in food[S].
[13]GB/T 5009.197-2003保健食品中盐酸硫胺素、盐酸吡哆醇、烟酸、烟酰胺和咖啡因的测定[S].GB/T 5009.197-2003 Determination of thiamine hydrochloride,pyridoxine hydrochloride,niacin,nicotinamide and caffeine in health foods[S].
[14]GB 7301-2017饲料添加剂中烟酰胺的测定[S].GB 7301-2017 Determination of nicotinamide in feed additives[S].
[15]GB/T 29664-2013化妆品中烟酸、烟酰胺的测定[S].GB/T 29664-2013 Determination of niacin and nicotinamide in cosmetics[S].
[16]NY/T 2130-2012饲料中烟酰胺的测定[S].NY/T 2130-2012 Determination of nicotinamide in feed[S].
[17]DB37/T 2140-2012预混合饲料中维生素B1、B2、B6和烟酰胺的同步测定[S].DB37/T 2140-2012 Simultaneous determination of vitamins B1,B2,B6 and nicotinamide in premixed feed[S].
[18]GB/T 27404-2008实验室质量控制规范食品理化检测[S].GB/T 27404-2008 Criterion on quality control of laboratories-Chemical testing of food[S].
[2]沈丽平.HPLC测定维生素B6片含量测定方法的探讨[J].海峡药学,2016,28(2):75-76.Shen LP.Discussion on the determination method of vitamin B6 tablets by HPLC[J].Strait Pharm J,2016,28(2):75-76.
[3]曹高娃,崔毅,张力,等.紫外分光光度法测定维生素B6[J].光谱实验室,2001,18(6):802-803.Cao GW,Cui Y,Zhang L,et al.Determination of vitamin B6 by ultraviolet spectrophotometry[J].Spectrosc Lab,2001,18(6):802-803.
[4]Galluzzi L,Kepp O,Vander-Heiden MG,et al.Metabolic targets for cancer therapy[J].Nat Rev Drug Discov,2013,12(11):829-846.
[5]Vora M,Ansari J,Shanti RM,et al.Increased nicotinamide phosphoribosyltransferase in rhabdomyosarcomas and leiomyosarcomas compared to skeletal and smooth muscle tissue[J].Anticancer Res,2016,36(2):503-507.
[6]韩永利,李秋凤,李建国.烟酸对奶牛的营养作用[J].饲料博览,2002,(3):33-34.Han YL,Li QF,Li JG.Nutrition effect of niacin on dairy cows[J].Feed Expo,2002,(3):33-34.
[7]GB 5009.154-2016食品中维生素B6的测定[S].GB 5009.154-2016 Determination of vitamin B6 in food[S].
[8]GB 14753-2010食品添加剂中维生素B6的测定[S].GB 14753-2010 Determination of vitamin B6 in food additives[S]
[9]GB 7298-2017饲料添加剂中维生素B6的测定[S].GB 7298-2017 Determination of vitamin B6 in feed additives[S].
[10]GB/T 33309-2016化妆品中维生素B6的测定[S].GB/T 33309-2016 Determination of vitamin B6 in cosmetics[S].
[11]SN/T 0549-1996出口蜂王浆及干粉中维生素B6的测定[S].SN/T 0549-1996 Determination of vitamin B6 in exported royal jelly and dry powder[S].
[12]GB 5009.89-2016食品中烟酸和烟酰胺的测定[S].GB 5009.89-2016 Determination of niacin and nicotinamide in food[S].
[13]GB/T 5009.197-2003保健食品中盐酸硫胺素、盐酸吡哆醇、烟酸、烟酰胺和咖啡因的测定[S].GB/T 5009.197-2003 Determination of thiamine hydrochloride,pyridoxine hydrochloride,niacin,nicotinamide and caffeine in health foods[S].
[14]GB 7301-2017饲料添加剂中烟酰胺的测定[S].GB 7301-2017 Determination of nicotinamide in feed additives[S].
[15]GB/T 29664-2013化妆品中烟酸、烟酰胺的测定[S].GB/T 29664-2013 Determination of niacin and nicotinamide in cosmetics[S].
[16]NY/T 2130-2012饲料中烟酰胺的测定[S].NY/T 2130-2012 Determination of nicotinamide in feed[S].
[17]DB37/T 2140-2012预混合饲料中维生素B1、B2、B6和烟酰胺的同步测定[S].DB37/T 2140-2012 Simultaneous determination of vitamins B1,B2,B6 and nicotinamide in premixed feed[S].
[18]GB/T 27404-2008实验室质量控制规范食品理化检测[S].GB/T 27404-2008 Criterion on quality control of laboratories-Chemical testing of food[S].