肠道病毒71型感染手足口病S100B基因多态性研究
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摘要
目的研究S100B基因rs9722位点基因多态性与肠道病毒71型(EV71)感染手足口病(HFMD)的相关性。方法以124例EV71感染HFMD患儿为研究对象,56例健康儿童作为对照组,两组均进行S100B基因rs9722位点基因多态性检测;并且74例HFMD患儿进行血清S100B蛋白检测。结果 S100B基因rs9722位点检出3种基因型:CC型、CT型和TT型,基因型频率分布符合Hardy-Weinberg平衡。与对照组比较,HFMD组患儿S100B基因rs9722位点TT基因型频率和T等位基因频率明显增高(P<0.01)。重症EV71感染相关HFMD患儿TT基因型频率和T等位基因频率均明显高于普通型患儿(P<0.05)。与痊愈组比较,HFMD不良预后组的S100B基因rs9722位点TT基因型频率和T等位基因频率明显增高(P<0.05)。74例HFMD患儿各基因型的血清S100B蛋白水平以TT基因型最高,CC型最低(P<0.01)。结论 S100B基因rs9722位点T等位基因可能是EV71感染HFMD发展为重症的危险因素。
Objective To investigate the association between rs9722 polymorphisms in the S100B gene and hand,foot and mouth disease(HFMD) caused by enterovirus 71.Methods A total of 124 HFMD children with enterovirus 71 infection were enrolled as subjects,and 56 healthy children were enrolled as control group.The rs9722 polymorphisms in the S100B gene were detected for both groups,and the serum level of S100B protein was measured for 74 HFMD children.Results The rs9722 locus of the S100B gene had three genotypes,CC,CT,and TT,and the genotype frequencies were in accordance with Hardy-Weinberg equilibrium.Compared with the control group,the HFMD group had significant increases in the frequencies of TT genotype and T allele(P<0.01).Children with severe HFMD caused by enterovirus 71 infection had significantly higher frequencies of TT genotype and T allele than those with moderate or mild HFMD(P<0.05).Compared with the cured patients,the patients with poor prognosis had significant increases in the frequencies of TT genotype and T allele in the rs9722 locus of the S100B gene(P<0.05).Among the 74 children with HFMD,the children with TT genotype had the highest serum level of S100B protein,and those with CC genotype had the lowest level(P<0.01).Conclusions T allele in the rs9722 locus of the S100B gene might be a risk factor for severe HFMD caused by enterovirus 71 infection.
Objective To investigate the association between rs9722 polymorphisms in the S100B gene and hand,foot and mouth disease(HFMD) caused by enterovirus 71.Methods A total of 124 HFMD children with enterovirus 71 infection were enrolled as subjects,and 56 healthy children were enrolled as control group.The rs9722 polymorphisms in the S100B gene were detected for both groups,and the serum level of S100B protein was measured for 74 HFMD children.Results The rs9722 locus of the S100B gene had three genotypes,CC,CT,and TT,and the genotype frequencies were in accordance with Hardy-Weinberg equilibrium.Compared with the control group,the HFMD group had significant increases in the frequencies of TT genotype and T allele(P<0.01).Children with severe HFMD caused by enterovirus 71 infection had significantly higher frequencies of TT genotype and T allele than those with moderate or mild HFMD(P<0.05).Compared with the cured patients,the patients with poor prognosis had significant increases in the frequencies of TT genotype and T allele in the rs9722 locus of the S100B gene(P<0.05).Among the 74 children with HFMD,the children with TT genotype had the highest serum level of S100B protein,and those with CC genotype had the lowest level(P<0.01).Conclusions T allele in the rs9722 locus of the S100B gene might be a risk factor for severe HFMD caused by enterovirus 71 infection.
引文
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[13]White M,Foulis AK,Smith G,et al.The role of S100 staining in the pathological assessment of perineural invasion in rectal cancer[J].Colorectal Dis,2013,11(3):278-286.
[14]张乐海,马丽霞,王世富.山东省部分地区脑炎与非脑炎手足口病肠道病毒71型VP1区基因特征[J].中国病原生物学杂志,2010,5(8):567-571.
[15]Zou R,Zhang G,Li S,et al.A functional polymorphism in IFNAR1 gene is associated with susceptibility and severity of HFMD with EV71 infection[J].Sci Rep,2015,5:18541.
[16]Li F,Liu XP,Li JA,et al.Correlation of an interleukin-4 gene polymorphism with susceptibility to severe enterovirus 71infection in Chinese children[J].Arch Virol,2015,160(4):1035-1042.
[2]孙晓峰,杨郁野,张宏义,等.开颅术后颅内感染患者脑脊液与血清S-100B蛋白含量的变化意义[J].中华医院感染学杂志,2016,26(6):1345-1347.
[3]王晶,殷亮,吕涌涛,等.S100B在癫癎患者脑脊液和血清中的表达[J].实用医学杂志,2016,32(20):3422-3424.
[4]王百灵,陈玉平,孔伶俐,等.血清S-100B蛋白水平与前循环进展性卒中关系的研究[J].中国老年保健医学,2016,14(3):14-16.
[5]Zhai J,Cheng L,Dong J,et al.S100B gene polymorphisms predict prefrontal spatial function in both schizophrenia patients and healthy individuals[J].Schizophr Res,2012,134(1):89-94.
[6]杨坤,谢光荣,胡义秋,等.S100B基因rs9722多态性与抑郁症的关联分析[J].中国神经精神疾病杂志,2008,34(5):305-307.
[7]安妍,聂莹雪,赵传胜.107例病毒性脑炎患者近期预后相关因素分析[J].中国医科大学学报,2011,40(11):1014-1017.
[8]Staffa K,Ondruschka B,Franke H,et al.Cerebellar gene expression following human traumatic brain injury[J].J Neurotrauma,2012,29(17):2716-2721.
[9]Lo TY,Jones PA,Minns RA.Pediatric brain trauma outcome prediction using paired serum levels of inflammatory mediators and brain-specific proteins[J].J Neurotrauma,2009,26(9):1479-1487.
[10]王鹏高,施新革,赵文增.S100蛋白与体外循环脑损伤[J].新乡医学院学报,2006,23(4):426-428.
[11]刘希尧,张建生,丁永忠,等.广泛脑挫裂伤早期脑脊液S100B蛋白、IL-1β动态变化的实验研究[J].中国急救医学,2006,26(5):329-331.
[12]Laribi S,Kansao J,Borderie D,et al.S100B blood level measurement to exclude cerebral lesions after minor head injury:the multicenter STIC-S100 French study[J].Clin Chem Lab Med,2013,6(8):1-10.
[13]White M,Foulis AK,Smith G,et al.The role of S100 staining in the pathological assessment of perineural invasion in rectal cancer[J].Colorectal Dis,2013,11(3):278-286.
[14]张乐海,马丽霞,王世富.山东省部分地区脑炎与非脑炎手足口病肠道病毒71型VP1区基因特征[J].中国病原生物学杂志,2010,5(8):567-571.
[15]Zou R,Zhang G,Li S,et al.A functional polymorphism in IFNAR1 gene is associated with susceptibility and severity of HFMD with EV71 infection[J].Sci Rep,2015,5:18541.
[16]Li F,Liu XP,Li JA,et al.Correlation of an interleukin-4 gene polymorphism with susceptibility to severe enterovirus 71infection in Chinese children[J].Arch Virol,2015,160(4):1035-1042.