胃炎、食管炎和食管癌患者血清中人丝氨酸蛋白酶8的表达及临床意义
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摘要
目的探讨人丝氨酸蛋白酶8(PRSS8)在胃炎、食管炎和食管癌患者血清中的表达情况及意义。方法采集临床诊断为食管炎、食管癌和胃炎患者以及正常人群的血清样本,其中食管炎患者血清样本20例,食管癌患者血清样本6例,胃炎患者血清样本60例,正常血清样本28例。采用酶联免疫吸附法(ELISA)的方法测定各组中PRSS8的表达水平,并将食管炎患者组、食管癌患者组和胃炎患者组分别与正常组相比较。结果各组血清PRSS8表达水平差异有统计学意义(F=13.24,P<0.01)。食管炎患者组PRSS8表达水平[(4.58±1.67)μg/L]高于正常组[(3.52±1.49)μg/L],差异有统计学意义(LSD-t=2.59,P<0.05);食管癌患者组PRSS8表达水平[(5.05±0.57)μg/L]高于正常组[(3.52±1.49)μg/L],差异有统计学意义(LSD-t=2.54,P<0.05);食管癌患者组PRSS8表达水平[(5.05±0.57)μg/L]高于食管炎患者组[(4.58±1.67)μg/L],差异无统计学意义(LSD-t=0.83,P>0.05);胃炎患者组PRSS8表达水平[(5.39±1.04)μg/L]高于正常组[(3.52±1.49)μg/L],差异有统计学意义(LSD-t=6.22,P<0.05)。结论食管炎、食管癌和胃炎患者血清中PRSS8表达水平均增高,提示血清中的PRSS8可能与食管炎、食管癌和胃炎的发生发展相关。
Objective To study the expression level and significance of serum human protease serine 8( PRSS8) in the patient with gastritis,esophagitis and esophageal cancer. Methods The serum samples from healthy subjects and the patients diagnosed as esophagitis,esophageal cancer and gastritis were collected.The serum samples included 20 esophagitis serum samples,6 esophageal cancer serum samples,60 gastritis serum samples and 28 healthy serum samples.The expression level of PRSS8 was detected by ELISA assay.Then the serum PRSS8 levels from the esophagitis group,esophageal cancer group and the gastritis group were compared with the healthy group,respectively.Results The serum level of PRSS8 in esophagitis group [( 4.58±1.67) μg / L] was higher than that from the healthy group [( 3. 52 ± 1. 49) μg / L ],the difference was statistically significant( LSD-t = 2. 59,P < 0. 05). The serum level of PRSS8 in esophageal cancer group [( 5.05±0.57) μg / L] was higher than that from the healthy group [( 3.52± 1.49) μg / L],the difference was statistically significant( LSD-t = 2. 54,P < 0. 05). The serum level of PRSS8 in esophageal cancer group [( 5.05±0.57) μg / L] was higher than that from the esophagitis group [( 4.58± 1.67) μg / L],the difference was not statistically significant( LSD-t = 0.83,P >0.05).The level of serum PRSS8 in gastritis group [( 5.39±1.04) μg / L]was higher than that from the healthy group [( 3.52±1.49) μg / L],the difference was also statistically significant( LSD-t = 6. 22,P < 0. 05). Conclusion Serum PRSS8 expression levels are obviously increased in the patients with esophagitis,esophageal cancer and gastritis,which suggests that serum PRRS8 maybe relate to the development of esophagitis,esophageal cancer and gastritis.
Objective To study the expression level and significance of serum human protease serine 8( PRSS8) in the patient with gastritis,esophagitis and esophageal cancer. Methods The serum samples from healthy subjects and the patients diagnosed as esophagitis,esophageal cancer and gastritis were collected.The serum samples included 20 esophagitis serum samples,6 esophageal cancer serum samples,60 gastritis serum samples and 28 healthy serum samples.The expression level of PRSS8 was detected by ELISA assay.Then the serum PRSS8 levels from the esophagitis group,esophageal cancer group and the gastritis group were compared with the healthy group,respectively.Results The serum level of PRSS8 in esophagitis group [( 4.58±1.67) μg / L] was higher than that from the healthy group [( 3. 52 ± 1. 49) μg / L ],the difference was statistically significant( LSD-t = 2. 59,P < 0. 05). The serum level of PRSS8 in esophageal cancer group [( 5.05±0.57) μg / L] was higher than that from the healthy group [( 3.52± 1.49) μg / L],the difference was statistically significant( LSD-t = 2. 54,P < 0. 05). The serum level of PRSS8 in esophageal cancer group [( 5.05±0.57) μg / L] was higher than that from the esophagitis group [( 4.58± 1.67) μg / L],the difference was not statistically significant( LSD-t = 0.83,P >0.05).The level of serum PRSS8 in gastritis group [( 5.39±1.04) μg / L]was higher than that from the healthy group [( 3.52±1.49) μg / L],the difference was also statistically significant( LSD-t = 6. 22,P < 0. 05). Conclusion Serum PRSS8 expression levels are obviously increased in the patients with esophagitis,esophageal cancer and gastritis,which suggests that serum PRRS8 maybe relate to the development of esophagitis,esophageal cancer and gastritis.
引文
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[14]Sarojini S,Tamir A,Lim H,et al.Early detection biomarkers for ovarian cancer[J].J Oncol,2012:709049.
[15]Yan BX,Ma JX,Zhang J,et al.Prostasin may contribute to chem oresistance,repress cancer cells in ovarian cancer,and is involved in the signaling pathways of CASP/PAK2-p34/actin[J].Cell Death Dis,2014(5):e995.
[16]Chen PX,Li QY,Yang Z.Axl and prostasin are biomarkers for prognosis of ovarian adenocarcinoma[J].Ann Diagn Pathol,2013,17(5):425-429.
[2]Hooper JD,Bowen N,Marshall H,et al.Localization,expression and genomic structure of the gene encoding the human serine protease testisin[J].Biochim Biophys Acta,2000,1492(1):63-71.
[3]Yu JX,Chao L,Ward DC,et al.Structure and chromosomal localization of the human prostasin(PRSS8)gene[J].Genomics,1996,32(3):334-340.
[4]Yu JX,Chao L,Chao J.Molecular cloning,tissue-specific expression,and cellular localization of human prostasin mRNA[J].J Biol Chem,1995,270(22):13483-13489.
[5]Leyvraz C,Charles RP,Rubera I,et al.The epidermal barrier function is dependent on the serine protease CAP1/Prss8[J].J Cell Biol,2005,170(3):487-496.
[6]Buzza MS,Martin EW,Driesbaugh KH,et al.Prostasin is required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway[J].J Biol Chem,2013,288(15):10328-10337.
[7]Chen LM,Hatfield ML,Fu YY,et al.Prostasin regulates i NOS and cyclin D1 expression by modulating protease-activated receptor-2signaling in prostate epithelial cells[J].Prostate,2009,69(16):1790-1801.
[8]Chen LM,Wang C,Chen M,et al.Prostasin attenuates inducible nitric oxide synthase expression in lipopolysaccharide-induced urinary bladder inflammation[J].Am J Physiol Renal Physiol,2006,291(3):F567-577.
[9]Sakashita K,Mimori K,Tanaka F,et al.Clinical significance of low expression of prostasin mRNA in human gastric cancer[J].J Surg Oncol,2008,98(7):559-564.
[10]Takahashi S,Suzuki S,Inaguma S,et al.Down-regulated expression of prostasin in high-grade or hormone-refractory human prostate cancers[J].Prostate,2003,54(3):187-193.
[11]Chen LM,Verity NJ,Chai KX.Loss of prostasin(PRSS8)in human bladder transitional cell carcinoma cell lines is associated with epithelial-mesenchymal transition(EMT)[J].BMC cancer,2009(9):377.
[12]Chen LM,Chai KX.Prostasin serine protease inhibits breast cancer invasiveness and is transcriptionally regulated by promoter DNA methylation[J].Int J Cancer,2002,97(3):323-329.
[13]López-Otín C,Matrisian LM.Emerging roles of proteases in tumour suppression[J].Nat Rev Cancer,2007,7(10):800-808.
[14]Sarojini S,Tamir A,Lim H,et al.Early detection biomarkers for ovarian cancer[J].J Oncol,2012:709049.
[15]Yan BX,Ma JX,Zhang J,et al.Prostasin may contribute to chem oresistance,repress cancer cells in ovarian cancer,and is involved in the signaling pathways of CASP/PAK2-p34/actin[J].Cell Death Dis,2014(5):e995.
[16]Chen PX,Li QY,Yang Z.Axl and prostasin are biomarkers for prognosis of ovarian adenocarcinoma[J].Ann Diagn Pathol,2013,17(5):425-429.