陈氏刺棘虫感染对洄游型刀鲚肠道微生物群落的影响
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摘要
于2017年洄游汛期内采集刀鲚(Coilianasus)样本,解剖后选取6尾感染陈氏刺棘虫(Acanthosentischeni)的刀鲚样本作为感染组,同时选取6尾未感染刀鲚作为对照组,采用16S rDNA高通量测序技术研究陈氏刺棘虫感染对刀鲚肠道微生物群落结构的影响。结果共鉴定出23个细菌门,其中对照组包含22个门,感染组鉴定出18个门,其中疣微菌门(Verrucomicrobia)只在感染组刀鲚中检测到;两组刀鲚肠道中主要的微生物类群均为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和软壁菌门(Tenericutes),且相对丰度总量均高于85%;感染组中厚壁菌门、梭杆菌门(Fusobacteria)和纤维杆菌门(Fibrobacteres)平均含量高于对照组,其余菌门相对丰度含量与对照组相比均呈下降趋势,但两组间微生物组成比例没有显著差异(P>0.05)。此外,在已明确的130个科中,厚壁菌门中的梭菌科(Clostridiaceae)、消化链球菌科(Peptostreptococcaceae)、乳杆菌科(Lactobacillaceae)和变形菌门中的肠杆菌科(Enterobacteriaceae)含量高于对照组(P<0.05),紫单胞菌科(Porphyromonadaceae)和毛螺菌科(Lachnospiraceae)中的Eisenbergiella丰富度在感染组刀鲚肠道中显著更高(P<0.05);在群落多样性水平上,对照组和感染组间Shannon指数、Chao1指数和Simpson指数差异不显著(P>0.05)。虽然陈氏刺棘虫感染对肠道微生物的群落结构和物种多样性影响不显著,但是在特定微生物物种的组成上存在显著差异。
Coilia nasus samples were collected during the migration flood season of 2017. After dissection, six samples of C. nasus infected with Acanthosentis cheni were selected as the infected group, and six samples that were uninfected were selected as the control group to determine the effect of A. cheni infection on the intestinal microbial community structure of C. nasus. A total of twenty-three bacterial phyla were identified. Twenty-two phyla were identified in the control group and eighteen phyla were identified in the infected group, among which Verrucomicrobia was only detected in the infected group. The main bacteria phyla in the intestinal tract of C.nasus were Proteobacteria, Firmicutes, and Tenericutes, with a total relative abundance of more than 85%. The average content of Firmicutes, Fusobacteria, and Fibrobacteres in the infected group was higher than that in the control group, and the relative abundance of other bacteria phyla was lower than that in the control group, but there was no significant difference between the two groups(P>0.05). Comparison of the relative abundance of bacteria at the family level showed that the proportions of Clostridiaceae, Peptostreptococcaceae, Lactobacillaceae from Firmicutes, and Enterobacteriaceae in the Proteobacteria were higher than those in the control group. Abundance of Peptostreptococcaceae, Lactobacillaceae, and Enterobacteriaceae in the 130 families identified were higher than those in the control group. Porphyromonadaceae and Eisenbergiella were significantly more abundant in the intestinal tract of the infected group(P<0.05). At the community diversity level, there was no significant difference in Shannon index, Chao1 index, and Simpson index between the two groups(P>0.05). Although the effects of A. cheni infection on the community structure and species diversity of intestinal microorganisms were not significant, there were significant differences in the composition of specific species.
Coilia nasus samples were collected during the migration flood season of 2017. After dissection, six samples of C. nasus infected with Acanthosentis cheni were selected as the infected group, and six samples that were uninfected were selected as the control group to determine the effect of A. cheni infection on the intestinal microbial community structure of C. nasus. A total of twenty-three bacterial phyla were identified. Twenty-two phyla were identified in the control group and eighteen phyla were identified in the infected group, among which Verrucomicrobia was only detected in the infected group. The main bacteria phyla in the intestinal tract of C.nasus were Proteobacteria, Firmicutes, and Tenericutes, with a total relative abundance of more than 85%. The average content of Firmicutes, Fusobacteria, and Fibrobacteres in the infected group was higher than that in the control group, and the relative abundance of other bacteria phyla was lower than that in the control group, but there was no significant difference between the two groups(P>0.05). Comparison of the relative abundance of bacteria at the family level showed that the proportions of Clostridiaceae, Peptostreptococcaceae, Lactobacillaceae from Firmicutes, and Enterobacteriaceae in the Proteobacteria were higher than those in the control group. Abundance of Peptostreptococcaceae, Lactobacillaceae, and Enterobacteriaceae in the 130 families identified were higher than those in the control group. Porphyromonadaceae and Eisenbergiella were significantly more abundant in the intestinal tract of the infected group(P<0.05). At the community diversity level, there was no significant difference in Shannon index, Chao1 index, and Simpson index between the two groups(P>0.05). Although the effects of A. cheni infection on the community structure and species diversity of intestinal microorganisms were not significant, there were significant differences in the composition of specific species.
引文
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[27]Wu S,Li R W,Li W,et al.Worm burden-dependent disruption of the porcine colon microbiota by Trichuris suis infection[J].PLoS ONE,2012,7(4):e35470.
[28]Kringel H,Roepstorff A.Trichuris suis population dynamics following a primary experimental infection[J].Veterinary Parasitology,2006,139(1):132-139.
[29]Yang G L,Xing X.Advances in the study on the interaction between helminths and intestinal flora[J].Chinese Journal of Microecology,2017,29(9):1087-1089.[杨桂连,邢鑫.蠕虫感染与肠道菌群相互作用的研究进展[J].中国微生态学杂志,2017,29(9):1087-1089.]
[30]Zaiss M M,Harris N L.The interaction of commensal intestinal bacteria with helminth parasites[J].Parasite Immunology,2015,38(1):5-11.
[31]Sun J F,Jia Y L.Advances in intestinal microbiota and immunomodulation in fish[J].Fisheries Science&Technology Information,2016,43(2):96-100.[孙敬锋,贾艳丽.鱼类肠道菌群与免疫调节研究进展[J].水产科技情报,2016,43(2):96-100.]
[2]Cooperative Organization for Investigation of Coilia ectenes Resources in the Yangtze River.Resource and utilization of Coilia ectenes in the Yangtze River[J].Freshwater Fisheries,1976(8):24-26.[长江刀鲚资源调查协作组.长江刀鱼资源及其利用[J].淡水渔业,1976(8):24-26.]
[3]Li W X,Wang G T.Helminth communities in Coilia nasus from anadromous,freshwater and landlocked stocks[J].Chinese Journal of Zoology,2014,49(2):233-243.[李文祥,王桂堂.洄游型、淡水型和陆封型刀鲚的寄生蠕虫群落结构[J].动物学杂志,2014,49(2):233-243.]
[4]Yu Y,Wu H S.Studies on the fauna of Acanthocephala of fishes from middle reaches of the Changjiang(Yangtze)River[J].Acta Hydrobiologica Sinica,1989,13(1):38-50.[余仪,伍惠生.长江中游鱼类寄生棘头虫区系的研究[J].水生生物学报,1989,13(1):38-50.]
[5]Macpherson A J,Harris N L.Interactions between commensal intestinal bacteria and the immune system[J].Nature Reviews Immunology,2004,4:478-485.
[6]Liu L L.The interaction between Trichinella spiralis and intestinal microbes[D].Changchun:Jilin University,2012.[刘丽丽.旋毛虫与肠道微生物间相互作用的研究[D].长春:吉林大学,2011.
[7]Ren Y X,Sun M Z,Qin Y H,et al.Relationship between parasite and host[J].Chinese Journal of Microecology,2014,24(1):1055-1056.[任一鑫,孙明忠,秦元华,等.寄生虫与宿主的关系[J].中国微生态学杂志,2014,24(1):1055-1056.]
[8]Li R W,Li W,Sun J,et al.The effect of helminth infection on the microbial composition and structure of the caprine abomasal microbiome[J].Scientific Reports,2016,6:20606.
[9]Ramanan D,Bowcutt R,Lee S C,et al.Helminth infection promotes colonization resistance via type 2 immunity[J].Science,2016,352(6285):608-612.
[10]Broadhurst M J,Ardeshir A,Kanwar B,et al.Therapeutic helminth infection of macaques with idiopathic chronic diarrhea alters the inflammatory signature and mucosal microbiota of the colon[J].PLoS Pathogens,2012,8(11):e1003000.
[11]Edgar R C,Haas B J,Clemente J C,et al.UCHIME improves sensitivity and speed of chimera detection[J].Bioinformatics,2011,27(16):2194-2200.
[12]Muyzer G.Denaturing gradient gel electrophoresis(DGGE)in microbial ecology:Electrophoresis-Principles and Basics[D].2004,3.4.4:743-769.
[13]Osborn A M,Moore E R B,Timmis K N.An evaluation of terminal-restriction fragment length polymorphism(T-RFLP)analysis for the study of microbial community structure and dynamics[J].Environmental Microbiology,2010,2(1):39-50.
[14]Li T T,Li A H.Comparison of the community structure of three different kinds of intestinal microbes in fish by high-throughput sequencing[C]//Proceedings of the Member Conference and Academic Annual Meeting of Hubei and Wuhan Microbiology Society,2013.[李彤彤,李爱华.应用高通量测序技术比较三种不同鱼肠道微生物的群落结构[C]//湖北省暨武汉微生物学会会员代表大会暨学术年会,2013.]
[15]Dehler C E,Secombes C J,Sam M.Seawater transfer alters the intestinal microbiota profiles of atlantic salmon(Salmo salar L.)[J].Scientific Reports,2017,7:Article No.13877.
[16]Nie Z J,Xu G C,Du F K,et al.PCR-DGGE fingerprinting and diversity analysis of the predominant bacterial community in Coilia nasus[J].Acta Hydrobiologica Sinica,2015,39(5):1019-1026.[聂志娟,徐钢春,杜富宽,等.长江刀鲚体内菌群PCR-DGGE指纹图谱及多样性比较分析[J].水生生物学报,2015,39(5):1019-1026.]
[17]Nagalingam N A,Kao J Y,Young V B.Microbial ecology of the murine gut associated with the development of dextran sodium sulfate-induced colitis[J].Inflammatory Bowel Diseases,2015,17(4):917-926.
[18]Rausch S,Held J,Fischer A,et al.Small intestinal nematode infection of mice is associated with increased enterobacterial loads alongside the intestinal tract[J].PLoS ONE,2013,8(9):e74026.
[19]Ahn J,Sinha R,Pei Z H,et al.Human gut microbiome and risk for colorectal cancer[J].Journal of the National Cancer Institute,2013,105(24):1907-1911.
[20]Walk S T,Blum A M,Ewing S A,et al.Alteration of the murine gut microbiota during infection with the parasitic helminth Heligmosomoides polygyrus[J].Inflammatory Bowel Diseases,2010,16(11):1841-1849.
[21]Reynolds L A.Immune response and the intestinal microbiota in control of susceptibility to Heligmosomoides polygyrus[OL].[2013-06-29].https://www.era.lib.ed.ac.uk/handle/1842/7960.
[22]Sakamoto M.The Family Porphyromonadaceae[M].Heidelberg:Springer,2014:811-824.
[23]Jousimies-Somer H R,Summanen P,Citron D M,et al.Wadsworth-KTL Anaerobic Bacteriology Manual[M].Belmont:Star Publisher,2002.
[24]Amir I,Bouvet P,Legeay C,et al.Eisenbergiella tayi gen.nov.,sp.nov.,isolated from human blood[J].International Journal of Systematic and Evolutionary Microbiology,2014,64(3):907-914.
[25]Cooper P,Walker A W,Reyes J,et al.Patent human infections with the whipworm,Trichuris trichiura,are not associated with alterations in the faecal microbiota[J].PLoS ONE,2013,8(10):e76573.
[26]Cantacessi C,Giacomin P,Croese J,et al.Impact of experimental hookworm infection on the human gut microbiota[J].Journal of Infectious Diseases,2014,210(9):1431-1434.
[27]Wu S,Li R W,Li W,et al.Worm burden-dependent disruption of the porcine colon microbiota by Trichuris suis infection[J].PLoS ONE,2012,7(4):e35470.
[28]Kringel H,Roepstorff A.Trichuris suis population dynamics following a primary experimental infection[J].Veterinary Parasitology,2006,139(1):132-139.
[29]Yang G L,Xing X.Advances in the study on the interaction between helminths and intestinal flora[J].Chinese Journal of Microecology,2017,29(9):1087-1089.[杨桂连,邢鑫.蠕虫感染与肠道菌群相互作用的研究进展[J].中国微生态学杂志,2017,29(9):1087-1089.]
[30]Zaiss M M,Harris N L.The interaction of commensal intestinal bacteria with helminth parasites[J].Parasite Immunology,2015,38(1):5-11.
[31]Sun J F,Jia Y L.Advances in intestinal microbiota and immunomodulation in fish[J].Fisheries Science&Technology Information,2016,43(2):96-100.[孙敬锋,贾艳丽.鱼类肠道菌群与免疫调节研究进展[J].水产科技情报,2016,43(2):96-100.]