基于生物组织偏振特性的肝癌检测方法
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摘要
肝细胞性肝癌属于原发性肝癌的一种,在我国发病率较高,且发病隐匿。癌变组织的组织结构和细胞形态会发生改变,这种亚波长微观结构的改变可以通过生物组织的偏振特性进行表达。根据光与生物组织相互作用原理,设计生物组织偏振成像系统,对30μm厚的未染色肝细胞性肝癌切片进行成像,借助与组织作用后背向散射光的偏振差异来表征生物组织的结构差异。实验结果表明,在各起偏角度的线偏振度图像中,肝癌组织与肝硬化组织的对比度较普通强度图像平均提升91%,最高提升155%,在切片较厚、未染色条件下线偏振度成像方法具有区分癌变组织和非癌变组织的能力,为肝癌的病理检测提供了一种新的思路。
Hepatocellular carcinoma is one of the primary liver cancer,which has a high and hidden incidence in China. The tissue structure and cell morphology of cancerous tissue changes,this sub-wavelength microstructure change can be expressed by the polarization properties of biological tissue. According to the relation of interaction between light and biological tissue,the biomechanical polarization imaging system was designed to image 30 μm thick non-stained pathological section of hepatocellular carcinoma,the structural differences of biological tissue could be characterized by the difference of the backscattered light's polarization state. The results show that in the different angle of incidence,the contrast ratio of liver cancer tissue and liver cirrhosis tissue in degree of linear polarization images were 91% higher than that in intensity images on average,and 155% was the highest. Under the condition of thick and non-staining,the cancerous tissues and non-cancerous tissues can be distinguished by linear polarization imaging method,which provides a new way of the pathological detection in hepatocellular carcinoma.
Hepatocellular carcinoma is one of the primary liver cancer,which has a high and hidden incidence in China. The tissue structure and cell morphology of cancerous tissue changes,this sub-wavelength microstructure change can be expressed by the polarization properties of biological tissue. According to the relation of interaction between light and biological tissue,the biomechanical polarization imaging system was designed to image 30 μm thick non-stained pathological section of hepatocellular carcinoma,the structural differences of biological tissue could be characterized by the difference of the backscattered light's polarization state. The results show that in the different angle of incidence,the contrast ratio of liver cancer tissue and liver cirrhosis tissue in degree of linear polarization images were 91% higher than that in intensity images on average,and 155% was the highest. Under the condition of thick and non-staining,the cancerous tissues and non-cancerous tissues can be distinguished by linear polarization imaging method,which provides a new way of the pathological detection in hepatocellular carcinoma.
引文
1路绍军.光波偏振态的静态测量研究.科学技术与工程,2016;16(25):190-192Lu Shaojun.Research on static measurement of the polarization state of light.Science Technology and Engineering,2016;16(25):190-192
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8杜娥.偏振散射特征定量检测与组织病变诊断机理研究.北京:清华大学,2014Du E.Research on quantitative detection of polarized scattering characteristics and diagnostic mechanism of tissue lesions.Beijing:Tsinghua University,2014
9徐可欣,高峰,赵会娟.生物医学光子学.北京:科学出版社,2011:9-11Xu Kexin,Gao Feng,Zhao Huijuan.Biomedical photonics.Beijing:Science Press,2011:9-11
10 Hielscher A H,Mourant J R,Bigio I J.Influence of particle size and concentration on the diffuse backscattering of polarized light from tissue phantoms and biological cell suspensions.Applied Optics,1997;36(1):125-135
11 He H,Zeng N,Liao R,et al.Application of sphere-cylinder scattering model to skeletal muscle.Optics Express,2010;18(14):15104-15112
12廖延彪.偏振光学.北京:科学出版社,2003:47-55Liao Yanbiao,Polarized optics.Beijing:Science Press,2003:47-55
13蒋啸宇,曾楠,何永红,等.基于旋转偏振角的线偏振扫描成像方法研究.生物化学与生物物理进展,2007;34(6):659-663Jiang Xiaoyu,Zeng Nan,He Yonghong,et al.Investigation of linear polarization difference imaging based on rotation of incident and backscattered polarization angles.Progress in Biochemistry and Biophysics,2007;34(6):659-663
2 Jacques S L,Roman J R,Lee K.Imaging superficial tissues with polarized light.Lasers in Surgery&Medicine,2000;26(2):119-129
3 Pierangelo A,Nazac A,Benali A,et al.Polarimetric imaging of uterine cervix:a case study.Optics Express,2013;21(12):14120-14130
4何宏辉,曾楠,廖然,等.偏振光成像技术用于肿瘤病变检测的研究进展.生物化学与生物物理进展,2015;(5):419-433He Honghui,Zeng Nan,Liao Ran,et al.Advances in the study of polarized light imaging in the detection of tumor lesions.Progress in Biochemistry and Biophysics,2015;(5):419-433
5 Anderson R R.Polarized light examination and photography of the skin.Archives of Dermatology,1991;127(7):1000-1005
6 Jacques S L,Ramellaroman J C,Lee K.Imaging skin pathology with polarized light.Journal of Biomedical Optics,2002;7(3):329-340
7 Deng Y,Zeng S Q,Luo Q M,et al.Numerical study of the effect of scatterer sizes and distributions on multiple backscattered intensity patterns of polarized light.Opt Lett,2008;33(1):77-79
8杜娥.偏振散射特征定量检测与组织病变诊断机理研究.北京:清华大学,2014Du E.Research on quantitative detection of polarized scattering characteristics and diagnostic mechanism of tissue lesions.Beijing:Tsinghua University,2014
9徐可欣,高峰,赵会娟.生物医学光子学.北京:科学出版社,2011:9-11Xu Kexin,Gao Feng,Zhao Huijuan.Biomedical photonics.Beijing:Science Press,2011:9-11
10 Hielscher A H,Mourant J R,Bigio I J.Influence of particle size and concentration on the diffuse backscattering of polarized light from tissue phantoms and biological cell suspensions.Applied Optics,1997;36(1):125-135
11 He H,Zeng N,Liao R,et al.Application of sphere-cylinder scattering model to skeletal muscle.Optics Express,2010;18(14):15104-15112
12廖延彪.偏振光学.北京:科学出版社,2003:47-55Liao Yanbiao,Polarized optics.Beijing:Science Press,2003:47-55
13蒋啸宇,曾楠,何永红,等.基于旋转偏振角的线偏振扫描成像方法研究.生物化学与生物物理进展,2007;34(6):659-663Jiang Xiaoyu,Zeng Nan,He Yonghong,et al.Investigation of linear polarization difference imaging based on rotation of incident and backscattered polarization angles.Progress in Biochemistry and Biophysics,2007;34(6):659-663