植物蛋白激酶介导的非生物胁迫和激素信号转导途径的研究进展
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摘要
干旱、盐渍、低温和高温等非生物胁迫严重影响植物的生长发育和作物的产量。在长期的进化过程中,植物逐渐形成了对外部刺激快速感知和主动适应的能力,其中植物体内逆境信号的传递在植物快速感知外部刺激和主动适应非生物胁迫过程中起着非常重要的作用。蛋白激酶和蛋白磷酸酶催化的蛋白质磷酸化和去磷酸化是植物体内存在的最普遍且最重要的信号转导调节方式。其中,蛋白激酶的主要作用是将ATP或GTP上的γ磷酸基团转移到特定的底物蛋白上,使蛋白磷酸化,被磷酸化的蛋白发挥相应的生理功能。近年来,利用生物技术和基因工程等手段从细胞、分子水平上研究有关蛋白激酶的抗逆机理,通过基因沉默、基因过表达等策略提高植物的抗逆性成为国内外抗逆分子生物学与分子育种学研究的热点。本文主要对植物蛋白激酶在介导非生物胁迫和激素信号通路中的作用进行综述,为进一步研究植物蛋白激酶功能提供有价值的信息。
Abiotic stresses,such as drought,high salt,and low temperature,have a strong impact on crop growth and development and yield production. Plants gradually formed special capacity to rapidly perceive and adapt to external stimuli in the long-term evolution process,in which plant protein kinases play very important roles. Protein kinase and protein phosphatase catalyzing phosphorylation and dephosphorylation are ones of the most common and most important ways in signal transduction regulation in plants. And,the main effect of protein kinase is phosphorylating proteins by transferring γ-groups from ATP or GTP to a specific substrate protein,and then phosphorylated proteins play the corresponding physiological function. In recent years,research on mechanism of protein kinases by using bio-technology and genetic engineering resistance of plants via silencing or over-expressing genes has become a hot spot in molecular biology and molecular breeding. This article mainly summarizes the role of plant protein kinases in mediating abiotic stress and hormones,and will provide useful information for further research on plant protein kinase.
Abiotic stresses,such as drought,high salt,and low temperature,have a strong impact on crop growth and development and yield production. Plants gradually formed special capacity to rapidly perceive and adapt to external stimuli in the long-term evolution process,in which plant protein kinases play very important roles. Protein kinase and protein phosphatase catalyzing phosphorylation and dephosphorylation are ones of the most common and most important ways in signal transduction regulation in plants. And,the main effect of protein kinase is phosphorylating proteins by transferring γ-groups from ATP or GTP to a specific substrate protein,and then phosphorylated proteins play the corresponding physiological function. In recent years,research on mechanism of protein kinases by using bio-technology and genetic engineering resistance of plants via silencing or over-expressing genes has become a hot spot in molecular biology and molecular breeding. This article mainly summarizes the role of plant protein kinases in mediating abiotic stress and hormones,and will provide useful information for further research on plant protein kinase.
引文
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[3]Walker J C.Structure and function of the receptor-like protein kinases of higher plants[J].Plant Mo1 Biol,1994,26:1599-1609
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[24]Kim B G,Waadt R,Cheong Y H,et al.The calcium sensor CBL10 mediates salt tolerance by regulating ion homeostasis in Arabidopsis[J].Plant J,2007,52(3):473-484
[25]Yang Y,Tang R J,Jiang C M,et al.Overexpression of the Pt SOS2gene improves tolerance to salt stress in transgenic poplar plants[J].Plant Biotechnol J,2015,13(7):962-973
[26]Urao T,Katagiri T,Mizoguchi T,et al.Two gene that encode Ca2+-dependent protein kinase are induced by drought and highsalt in Arabidopsis thaliana[J].Mol Gen Genet,1994,244(4):331-340
[27]Xu J,Tian Y S,Peng R H,et al.At CPK6,a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis[J].Planta,2010,231(6):1251-1260
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[33]Romeis T,Ludwig A A,Martin R,et al.Calcium-dependent protein kinases play an essential role in a plant defence response[J].EMBO J,2001,20:5556-5567
[34]Saijo Y,Hata S,Kyozuka J,et al.Overexpression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants[J].Plant J,2000,23:319-327
[35]Dubrovina,A S,Kiselev,K V,Khristenko V S,et al.Va CPK20,a calcium-dependent protein kinase gene of wild grapevine Vitis amurensis Rupr.,mediates cold and drought stress tolerance[J].J Plant Physiol,2015,185:1-12
[36]Zhao R,Sun H,Zhao N et al.The Arabidopsis Ca(2)(+)-dependent protein kinase CPK27 is required for plant response to salt-stress[J].Gene,2015,563(2):203-214
[37]Wei S Y,Hu W,Deng X M,et al.A rice calcium-dependent protein kinase Os CPK9 positively regulates drought stress tolerance and spikelet fertility[J].BMC Plant Biol,2014,14:133
[38]Nagy S K,Darula Z,Kállai B M,et al.Activation of At MPK9through autophosphorylation that makes it independent of the canonical MAPK cascades[J].Biochem J,2015,467(1):167-175
[39]Mehlmer N,Wurzinger B,Stael S,et al.The Ca(2+)-dependent protein kinase CPK3 is required for MAPK-independent saltstress acclimation in Arabidopsis[J].Plant J,2010,63(3):484-498
[40]Xu D B,Chen M,Ma Y N,et al.A G-ProteinβSubunit,AGB1,negatively regulates the ABA response and drought tolerance by down-regulating At MPK6-related pathway in Arabidopsis[J].PLo S One,2015,10:e0116385
[41]Kumar K,Sinha A K.Overexpression of constitutively active mitogen activated protein kinase kinase 6 enhances tolerance to salt stress in rice[J].Rice,2013,6(1):25
[42]Ove cka M,TakácT,Komis G,et al.Salt-induced subcellular kinase relocation and seedling susceptibility caused by overexpression of Medicago SIMKK in Arabidopsis[J].J Exp Bot,2014,65(9):2335-2350
[43]Qin F,Shinozaki K,Yamaguchi-Shinozaki K.Achievements and challenges in understanding plant abiotic stress responses and tolerance[J].Plant Cell Physiol,2011,52:1569-1582
[44]Li J M,Chory J.A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction[J].Cell,1997,90:929-938
[45]Wang Z Y,Seto H,Fujioka S,et al.BRI1 is a critical component of a plasma-membrane receptor for plant steroids[J].Lett Nat,2001,410:381-383
[46]Li J,Wen J,Lease K A,et al.BAK1,an Arabidopsis LRR receptor-like protein kinase,interacts with BRI1 and modulates brassinosteroid signaling[J].Cell,2002,110:213-222
[47]Wang X,Chory J.Brassinosteroids regulate dissociation of BKI1,a negative regulator of BRI1 signaling,from the plasma membrane[J].Science,2006,313(5790):1118-1122
[48]Kim T W,Guan S,Burlingame A L,et al.The CDG1 kinase mediates brassinosteroid signal transduction from BRI1 receptor kinase to BSU1 phosphatase and GSK3-like kinase BIN2[J].Mol Cell,2011,43:561-571
[49]Ye H,Li L,Guo H,et al.MYBL2 is a substrate of GSK3-like kinase BIN2 and acts as a corepressor of BES1 in brassinosteroid signaling pathway in Arabidopsis[J].PNAS,2012,109:20142-20147
[50]Yin Y,Vafeados D,Tao Y,et al.A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis[J].Cell,2005,120:249-259
[51]Yin Y,Wang Z Y,Mora-Garcia S,et al.BES1 accumulates in the nucleus in response to brassinosteroids to regulate gene expression and promote stem elongation[J].Cell,2002,109:181-191
[52]Wang Z Y,Nakano T,Gendron J,et al.Nuclear-localized BZR1 mediates brassinosteroid-induced growth and feedback suppression of brassinosteroid biosynthesis[J].Dev Cell,2002,2:505-513
[53]Li L,Deng X W.It runs in the family:regulation of brassinosteroid signaling by the BZR1-BES1 class of transcription factors[J].Trends Plant Sci,2005,10:266-268
[54]Kim T W,Wang Z Y.Brassinosteroid signal transduction from receptor kinases to transcription factors[J].Annu Rev Plant Biol,2010,61:681-704
[55]Shang Y,Yan L,Liu Z Q,et al.The Mg-chelatase H subunit of Arabidopsis antagonizes a group of WRKY transcription repressors to relieve ABA-responsive genes of inhibition[J].Plant Cell,2010,22:1909-1935
[56]Ng L M,Soon F F,Zhou X E,et al.Structural basis for basal activity and autoactivation of abscisic acid(ABA)signaling SnRK2 kinases[J].Proc Natl Acad Sci USA,2011,108:21259-21264
[57]Umezawa T.Systems biology approaches to abscisic acid signaling[J].J Plant Res,2011,124:539-548
[58]Dai M,Xue Q,Mccray T,et al.The PP6 phosphatase regulates ABI5 phosphorylation and abscisic acid signaling in Arabidopsis[J].Plant Cell,2013,25:517-534
[59]Nemhauser J L,Hong F,Chory J.Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses[J].Cell,2006,126:467-475
[60]Ephritikhine G,Fellner M,Vannini C,et al.The sax1 dwarf mutant of Arabidopsis thaliana shows altered sensitivity of growth responses to abscisic acid,auxin,gibberellins and ethylene and is partially rescued by exogenous brassinosteroid[J].Plant J,1999,18:303-314
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[63]杨乐,徐兆师,刘生祥,等.植物抗逆相关蛋白激酶的结构与功能[J].植物遗传资源学报,2013,14(4):659-667
[2]Ferreira P C,Hemerly A S,Villarroel R,et al.The Arabidopsis functional homolog of the p34cdc2 protein kinase[J].Plant Cell,1991,3(5):531-540
[3]Walker J C.Structure and function of the receptor-like protein kinases of higher plants[J].Plant Mo1 Biol,1994,26:1599-1609
[4]Lee S K,Kim B G,Kwon T R,et al.Overexpression of the mitogen-activated protein kinase gene Os MAPK33 enhances sensitivity to salt stress in rice(Oryza sativa L.)[J].J Biosci,2011,36(1):139-151
[5]Xing H T,Guo P,Xia X L,et al.Pd ERECTA,a leucine-rich repeat-like kinase of polar,confers enhanced water use efficiency in Arabidopsis[J].Planta,2011,234:229-241
[6]Wu C W,Chi C W,Lin W C.Gastric cancer:prognostic and diagnostic advances[J].Expert Rev Mol Med,2002,4(6):1-12
[7]Hanks S K,Quinn A M.Protein kinase catalytic domain sequence database:identification of conserved features of primary structure and classification of family members[J].Methenzymol,1991,200:38-62
[8]Endicott J A,Noble M E,Johnson L N.The structural basis for control of eukaryotic protein kinases[J].Annu Rev Biochem,2012,81:587-613
[9]裴丽丽,郭玉华,徐兆师,等.植物逆境胁迫相关蛋白激酶的研究进展[J].西北植物学报,2012,30(5):1052-1061
[10]蔡冲,吕均良,陈昆松.蛋白激酶的研究(综述)[J].亚热带植物科学,2002,31(1):63-67
[11]张春宝,赵丽梅,赵洪锟,等.植物蛋白激酶研究进展[J].生物技术通报,2011(10):17-23
[12]Hanks S K,Hunter T.Protein kinases 6.The eukaryotic protein kinase superfamily:kinase(catalytic)domain structure and classification[J].FASEB J,1995,9:576-596
[13]Liu P,Xu Z S,Lu P,et al.A wheat PI4K gene whose product possesses threonine autophophorylation activity confers tolerance to drought and salt in Arabidopsis[J].J Exp Bot,2013,64(10):2915-2927
[14]Shi C C,Feng C C,Yang M M,et al.Overexpression of the receptor-like protein kinase genes AtRPK1 and OsRPK1 reduces the salt tolerance of Arabidopsis thaliana[J].Plant Sci,2014,217-218:63-70
[15]Park S,Moon J C,Park Y C,et al.Molecular dissection of the response of a rice leucine-rich repeat receptor-like kinase(LRR-RLK)gene to abiotic stresses[J].J Plant Physiol,2014,171:1645-1653
[16]Nilsson L,Muller R,Nielsen T H.Increased expression of the MYB-related transcription factor,PHR1,leads to enhanced phosphate uptake in Arabidopsis thaliana[J].Plant Cell Environ,2007,30:1499-1512
[17]商玲.盐角草钾离子通道蛋白基因Se AKT1的克隆与表达[D].大连:大连理工大学,2013
[18]Xu J,Li H D,Chen L Q,et al.A protein kinase,interacting with two calcineurin B-like proteins,regulates K+transporter AKT1 in Arabidopsis[J].Cell,2006,125(7):1347-1360
[19]Wu S J,Ding L,Zhu J K.SOS1,a Genetic locus essential for salt tolerance and potassium acquisition[J].Plant Cell,1996,8(4):617-627
[20]Shi H,Ishitani M,Kim C,et al.The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+antiporter[J].Proc Natl Acad Sci USA,2000,97(12):6896-6901
[21]Zhu J K,Liu J,Xiong L.Genetic analysis of salt tolerance in Arabidopsis.Evidence for a critical role of potassium nutrition[J].Plant Cell,1998,10(7):1181-1191
[22]Guo Y,Halfter U,Ishitani M,et al.Molecular characterization of functional domains in the protein kinase SOS2 that is required for plant salt tolerance[J].Plant Cell,2001,13(6):1383-1400
[23]Gong D,Guo Y,Jagendorf A T,et al.Biochemical characterization of the Arabidopsis protein kinase SOS2 that functions in salt tolerance[J].Plant Physiol,2002,130(1):256-264
[24]Kim B G,Waadt R,Cheong Y H,et al.The calcium sensor CBL10 mediates salt tolerance by regulating ion homeostasis in Arabidopsis[J].Plant J,2007,52(3):473-484
[25]Yang Y,Tang R J,Jiang C M,et al.Overexpression of the Pt SOS2gene improves tolerance to salt stress in transgenic poplar plants[J].Plant Biotechnol J,2015,13(7):962-973
[26]Urao T,Katagiri T,Mizoguchi T,et al.Two gene that encode Ca2+-dependent protein kinase are induced by drought and highsalt in Arabidopsis thaliana[J].Mol Gen Genet,1994,244(4):331-340
[27]Xu J,Tian Y S,Peng R H,et al.At CPK6,a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis[J].Planta,2010,231(6):1251-1260
[28]Asano T,Hakata M,Nakamura H,et al.Functional characterisation of Os CPK21,a calcium-dependent protein kinase that confers salt tolerance in rice[J].Plant Mol Biol,2011,75(1-2):179-191
[29]Asano T,Hayashi N,Kobayashi M,et al.A rice calcium-dependent protein kinase Os CPK12 oppositely modulates salt-stress tolerance and blast disease resistance[J].Plant J,2012,69(1):26-36
[30]Urao T,Katagiri T,Mizoguchi T,et al.Two genes that encode Ca2+-dependent protein-kinases are induced by drought and high-salt stresses in Arabidopsis thaliana[J].Mol Genet Genomics,1994,244:331-340
[31]Patharkar O R,Cushman J C.A stress-induced calciumdependent protein kinase from Mesembryanthemum crystallinum phosphorylates a two-component pseudo-response regulator[J].Plant Cell,2000,24:679-691
[32]Yoon G M,Cho H S,Ha H J,et al.Characterization of Nt CDPK1,a calcium-dependent protein kinase gene in Nicotiana tabacum,and the activity of its encoded protein[J].Plant Mol Biol,1999,39:991-1001
[33]Romeis T,Ludwig A A,Martin R,et al.Calcium-dependent protein kinases play an essential role in a plant defence response[J].EMBO J,2001,20:5556-5567
[34]Saijo Y,Hata S,Kyozuka J,et al.Overexpression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants[J].Plant J,2000,23:319-327
[35]Dubrovina,A S,Kiselev,K V,Khristenko V S,et al.Va CPK20,a calcium-dependent protein kinase gene of wild grapevine Vitis amurensis Rupr.,mediates cold and drought stress tolerance[J].J Plant Physiol,2015,185:1-12
[36]Zhao R,Sun H,Zhao N et al.The Arabidopsis Ca(2)(+)-dependent protein kinase CPK27 is required for plant response to salt-stress[J].Gene,2015,563(2):203-214
[37]Wei S Y,Hu W,Deng X M,et al.A rice calcium-dependent protein kinase Os CPK9 positively regulates drought stress tolerance and spikelet fertility[J].BMC Plant Biol,2014,14:133
[38]Nagy S K,Darula Z,Kállai B M,et al.Activation of At MPK9through autophosphorylation that makes it independent of the canonical MAPK cascades[J].Biochem J,2015,467(1):167-175
[39]Mehlmer N,Wurzinger B,Stael S,et al.The Ca(2+)-dependent protein kinase CPK3 is required for MAPK-independent saltstress acclimation in Arabidopsis[J].Plant J,2010,63(3):484-498
[40]Xu D B,Chen M,Ma Y N,et al.A G-ProteinβSubunit,AGB1,negatively regulates the ABA response and drought tolerance by down-regulating At MPK6-related pathway in Arabidopsis[J].PLo S One,2015,10:e0116385
[41]Kumar K,Sinha A K.Overexpression of constitutively active mitogen activated protein kinase kinase 6 enhances tolerance to salt stress in rice[J].Rice,2013,6(1):25
[42]Ove cka M,TakácT,Komis G,et al.Salt-induced subcellular kinase relocation and seedling susceptibility caused by overexpression of Medicago SIMKK in Arabidopsis[J].J Exp Bot,2014,65(9):2335-2350
[43]Qin F,Shinozaki K,Yamaguchi-Shinozaki K.Achievements and challenges in understanding plant abiotic stress responses and tolerance[J].Plant Cell Physiol,2011,52:1569-1582
[44]Li J M,Chory J.A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction[J].Cell,1997,90:929-938
[45]Wang Z Y,Seto H,Fujioka S,et al.BRI1 is a critical component of a plasma-membrane receptor for plant steroids[J].Lett Nat,2001,410:381-383
[46]Li J,Wen J,Lease K A,et al.BAK1,an Arabidopsis LRR receptor-like protein kinase,interacts with BRI1 and modulates brassinosteroid signaling[J].Cell,2002,110:213-222
[47]Wang X,Chory J.Brassinosteroids regulate dissociation of BKI1,a negative regulator of BRI1 signaling,from the plasma membrane[J].Science,2006,313(5790):1118-1122
[48]Kim T W,Guan S,Burlingame A L,et al.The CDG1 kinase mediates brassinosteroid signal transduction from BRI1 receptor kinase to BSU1 phosphatase and GSK3-like kinase BIN2[J].Mol Cell,2011,43:561-571
[49]Ye H,Li L,Guo H,et al.MYBL2 is a substrate of GSK3-like kinase BIN2 and acts as a corepressor of BES1 in brassinosteroid signaling pathway in Arabidopsis[J].PNAS,2012,109:20142-20147
[50]Yin Y,Vafeados D,Tao Y,et al.A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis[J].Cell,2005,120:249-259
[51]Yin Y,Wang Z Y,Mora-Garcia S,et al.BES1 accumulates in the nucleus in response to brassinosteroids to regulate gene expression and promote stem elongation[J].Cell,2002,109:181-191
[52]Wang Z Y,Nakano T,Gendron J,et al.Nuclear-localized BZR1 mediates brassinosteroid-induced growth and feedback suppression of brassinosteroid biosynthesis[J].Dev Cell,2002,2:505-513
[53]Li L,Deng X W.It runs in the family:regulation of brassinosteroid signaling by the BZR1-BES1 class of transcription factors[J].Trends Plant Sci,2005,10:266-268
[54]Kim T W,Wang Z Y.Brassinosteroid signal transduction from receptor kinases to transcription factors[J].Annu Rev Plant Biol,2010,61:681-704
[55]Shang Y,Yan L,Liu Z Q,et al.The Mg-chelatase H subunit of Arabidopsis antagonizes a group of WRKY transcription repressors to relieve ABA-responsive genes of inhibition[J].Plant Cell,2010,22:1909-1935
[56]Ng L M,Soon F F,Zhou X E,et al.Structural basis for basal activity and autoactivation of abscisic acid(ABA)signaling SnRK2 kinases[J].Proc Natl Acad Sci USA,2011,108:21259-21264
[57]Umezawa T.Systems biology approaches to abscisic acid signaling[J].J Plant Res,2011,124:539-548
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