长链非编码RNA MAPKAPK5-AS1在肺癌组织中的表达及临床意义
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摘要
目的研究长链非编码RNA (long ncRNA,lncRNA) MAPKAPK5-AS1在肺癌组织中的表达及临床意义。方法收集143例经病理明确诊断的肺癌患者癌组织及配对癌旁正常肺组织标本和临床资料,real time PCR法检测组织中lncRNA MAPKAPK5-AS1的表达,分析其临床意义,通过Kaplan-Meier生存曲线法和Log Rank检验法分析其对患者预后意义。结果 143例肺癌组织中MAPKAPK5-AS1相对表达水平(0. 012 2±0. 000 7)明显比癌旁正常肺组织中相对表达水平(0. 007 1±0. 000 4)高,差异有统计学意义(t=5. 727,P <0. 01);高表达MAPKAPK5-AS1与肺癌患者年龄、TNM分期、淋巴结转移、远处转移均密切相关(P值均<0. 05),并且与预后不良密切相关(t=3. 97,P <0. 05)。结论肺癌组织中高表达MAPKAPK5-AS1与肿瘤转移密切相关,是潜在肺癌预后生物学指标之一。
Objective To study the expression and clinical implications of lncRNA MAPKAPK5-AS1 in lung cancer tissues.Methods Lung cancer tissues and paired normal lung tissues nearby lung cancer tissues were collected,along with patient' s clinical data. MAPKAPK5-AS1 expression in these tissues was detected by real time PCR. Prognostic significance was analyzed by Kaplan-Meier survival curve and Log Rank test. Results MAPKAPK5-AS1 expression was higher in the lung cancer tissues( 0. 012 2 ± 0. 000 7) than the paired normal lung tissues( 0. 007 1 ± 0. 000 4),with the difference of statistical significance( t = 5. 727,P < 0. 01). High expressed MAPKAPK5-AS1 was closely related to patient' s age,TNM stage,lymphatic metastasis,and distant metastasis( P < 0. 05),along with poor prognosis( t = 3. 97,P < 0. 05). Conclusion High expression of MAPKAPK5-AS1 in the lung cancer tissues is intimately related to cancer metastasis,and may be a potential biomarker for lung cancer.
Objective To study the expression and clinical implications of lncRNA MAPKAPK5-AS1 in lung cancer tissues.Methods Lung cancer tissues and paired normal lung tissues nearby lung cancer tissues were collected,along with patient' s clinical data. MAPKAPK5-AS1 expression in these tissues was detected by real time PCR. Prognostic significance was analyzed by Kaplan-Meier survival curve and Log Rank test. Results MAPKAPK5-AS1 expression was higher in the lung cancer tissues( 0. 012 2 ± 0. 000 7) than the paired normal lung tissues( 0. 007 1 ± 0. 000 4),with the difference of statistical significance( t = 5. 727,P < 0. 01). High expressed MAPKAPK5-AS1 was closely related to patient' s age,TNM stage,lymphatic metastasis,and distant metastasis( P < 0. 05),along with poor prognosis( t = 3. 97,P < 0. 05). Conclusion High expression of MAPKAPK5-AS1 in the lung cancer tissues is intimately related to cancer metastasis,and may be a potential biomarker for lung cancer.
引文
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[2] Gupta RA,Shah N,Wang KC,et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis[J].Nature,2010,464(7291):1071-1076.
[3] Schmidt LH,Spieker T,Koschmieder S,et al. The long noncoding MALAT-1 RNA indicates a poor prognosis in non-small cell lung cancer and induces migration and tumor growth[J]. J Thorac Oncol,2011,6(12):1984-1992.
[4] Jiang C,Yang Y,Yang Y,et al. LncRNA-HOTAIR affects tumorigenesis and metastasis of non-small cell lung cancer by upregulating miR-613[J]. Oncol Res,2017,26(5):725-734.
[5] Ou C,Li G. Long non-coding RNA TUG1:a novel therapeutic target in small cell lung cancer[J]. J Thorac Dis,2017,9(7):E644-E645.
[6] Torre LA,Bray F,Siegel RL,et al. Global cancer statistics,2012[J]. CA Cancer J Clin,2015,65(2):87-108.
[7] Loewen G,Jayawickramarajah J,Zhuo Y,et al. Functions of lncRNA HOTAIR in lung cancer[J]. J Hematol Oncol,2014,7:90.
[8] Niu Y,Ma F,Huang W,et al. Long non-coding RNA TUG1 is involved in cell growth and chemoresistance of small cell lung cancer by regulating LIMK2b via EZH2[J]. Mol Cancer,2017,16(1):5.
[9] Li N,Wang Y,Liu X,et al. Identification of Circulating Long Noncoding RNA HOTAIR as a Novel Biomarker for Diagnosis and Monitoring of Non-Small Cell Lung Cancer[J]. Technol Cancer Res Treat,2017,16(6):1060-1066.
[10] Li S,Mei Z,Hu HB,et al. The lncRNA MALAT1 contributes to non-small cell lung cancer development via modulating miR-124/STAT3 axis[J]. J Cell Physiol,2017,233(9):6679-6688.
[11] Yang X,Zhang W,Cheng SQ,et al. High expression of lncRNA GACAT3 inhibits invasion and metastasis of non-small cell lung cancer to enhance the effect of radiotherapy[J]. Eur Rev Med Pharmacol Sci,2018,22(5):1315-1322.
[12] Xia H,Qu XL,Liu LY,et al. LncRNA MEG3 promotes the sensitivity of vincristine by inhibiting autophagy in lung cancer chemotherapy[J]. Eur Rev Med Pharmacol Sci,2018,22(4):1020-1027.
[13] Zhou Y,Sheng B,Xia Q,et al. Association of long non-coding RNA H19 and microRNA-21 expression with the biological features and prognosis of non-small cell lung cancer[J]. Cancer Gene Ther,2017,24(8):317-324.
[14] Zhang J,Fan D,Jian Z,et al. Cancer Specific Long Noncoding RNAs Show Differential Expression Patterns and Competing Endogenous RNA Potential in Hepatocellular Carcinoma[J]. Plo S One,2015,10(10):e0141042.
[15] Wang W,Zhao Z,Yang F,et al. An immune-related lncRNA signature for patients with anaplastic gliomas[J]. J Neurooncol,2018,136(2):263-271.
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