非小细胞肺癌组织中HOX转录反义RNA的表达变化及意义
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摘要
目的观察HOX转录反义RNA(HOTAIR)在非小细胞肺癌(NSCLC)组织中的表达变化,并探讨其临床意义。方法收集125例NSCLC患者的肿瘤组织及其瘤旁正常肺组织,采用实时RT-PCR法检测HOTAIR的表达。检索高通量基因表达数据库(GEO)、Array Express数据库中NSCLC组织与HOTAIR表达相关的芯片数据。结果125例患者中,HOTAIR在NSCLC组织中的表达高于癌旁正常肺组织(P<0.05)。HOTAIR表达水平与NSCLC患者年龄、肿瘤直径、淋巴结转移、TNM分期有关(P均<0.05)。从数据库检索出的基因芯片显示HOTAIR表达水平与肿瘤病理类型不相关(P>0.05)。结论 HOTAIR在NSCLC组织中呈现高表达,可能参与NSCLC的发生发展。
Objective To observe the expression changes of HOX transcript antisense RNA( HOTAIR) in the nonsmall-cell lung cancer( NSCLC) tissues and to investigate its clinical significance. Methods NSCLC tissues and adjacent tissues from 125 patients were collected and the expression of HOTAIR was detected by RT-PCR. Microarray data on the correlations between HOTAIR expression and NSCLC tissues were retrieved from Gene Expression Omnibus( GEO) and Array Express database. Results The expression of HOTAIR in the NSCLC was significantly higher than that in the adjacent tissues( P < 0. 05). The expression of HOTAIR was closely related to age,tumor diameter,lymph node metastasis and TNM stage of NSCLC patients( all P < 0. 05). The microarray data from GEO Profiles demonstrated that the HOTAIR expression level was not related with pathological pattern( P > 0. 05). Conclusion HOTAIR is highly expressed in the NSCLC tissues,and its expression may take part in the occurrence and development of NSCLC.
Objective To observe the expression changes of HOX transcript antisense RNA( HOTAIR) in the nonsmall-cell lung cancer( NSCLC) tissues and to investigate its clinical significance. Methods NSCLC tissues and adjacent tissues from 125 patients were collected and the expression of HOTAIR was detected by RT-PCR. Microarray data on the correlations between HOTAIR expression and NSCLC tissues were retrieved from Gene Expression Omnibus( GEO) and Array Express database. Results The expression of HOTAIR in the NSCLC was significantly higher than that in the adjacent tissues( P < 0. 05). The expression of HOTAIR was closely related to age,tumor diameter,lymph node metastasis and TNM stage of NSCLC patients( all P < 0. 05). The microarray data from GEO Profiles demonstrated that the HOTAIR expression level was not related with pathological pattern( P > 0. 05). Conclusion HOTAIR is highly expressed in the NSCLC tissues,and its expression may take part in the occurrence and development of NSCLC.
引文
[1]陆海林,李燕,宁志强.紫杉醇脂质体联合卡铂治疗老年非小细胞肺癌29例临床观察[J].山东医药,2010,50(47):79-80.
[2]张冠中,焦顺昌.培美曲塞治疗非小细胞肺癌的研究进展[J].山东医药,2011,51(15):113-114.
[3]Liu JH,Chen G,Dang YW,et al.Expression and Prognostic Significance of lncRNA MALAT1 in Pancreatic Cancer Tissues[J].Asian Pac J Cancer Prev,2014,15(7):2971-2977.
[4]Emadi-Andani E,Nikpour P,Emadi-Baygi M,et al.Association of HOTAIR expression in gastric carcinoma with invasion and distant metastasis[J].Adv Biomed Res,2014,28(3):135.
[5]Rong M,He R,Dang Y,et al.Expression and clinicopathological significance of miR-146a in hepatocellular carcinoma tissues[J].Ups J Med Sci,2014,119(1):19-24.
[6]Chen G,Kronenberger P,Umelo IA,et al.Quantification of epidermal growth factor receptor T790M mutant transcripts in lung cancer cells by real-time reverse transcriptase-quantitative polymerase chain reaction[J].Anal Biochem,2010,398(2):266-268.
[7]刘敏,曾龙剑,姚树祥,等.LncRNA HOTAIR、HOTTIP及其下游HOXD10、HOXA13基因在肺癌中的表达研究[J].昆明医科大学学报,2014,35(10):49-52.
[8]林梦洁,陈志强,尹凌帝,等.长链非编码RNA HOTAIR对非小细胞肺癌迁移和侵袭能力的影响[J].临床肿瘤学杂志,2014,19(8):684-689.
[9]Liu XH,Liu ZL,Sun M,et al.The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in nonsmall cell lung cancer[J].BMC Cancer,2013,13:464.
[10]Nakaqawa T,Endo H,Yokoyama M,et al.Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer[J].Biochem Biophys Res Commun,2013,436(2):319-324.
[11]Zhao W,An Y,Liang Y,et al.Role of HOTAIR long noncoding RNA in metastatic progression of lung cancer[J].Eur Rev Med Pharmacol Sci,2014,18(13):1930-1936.
[12]Rinn JL,Kertesz M,Wang JK,et al.Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs[J].Cell,2007,129(7):1311-1323.
[13]Wan Y,Chang HY.HOTAIR:Flight of noncoding RNAs in cancer metastasis[J].Cell Cycle,2010,9(17):3391-3392.
[14]Shah N,Sukumar S.The Hox genes and their roles in oncogenesis[J].Nat Rev Cancer,2010,10(5):361-371.
[15]Li D,Feng J,Wu T,et al.Long intergenic noncoding RNA HOTAIR is overexpressed and regulates PTEN methylation in laryngeal squamous cell carcinoma[J].Am J Pathol,2013,182(1):64-70.
[16]Zeng ZY,Zhou YH,Zhang WL,et al.Gene expression profiling of nasopharyngeal carcinoma reveals the abnormally regulated Wnt signaling pathway[J].Hum Pathol,2007,38(1):120-133.
[17]Liu Z,Sun M,Lu K,et al.The long noncoding RNA HOTAIR contributes to cisplatin resistance ofhuman lung adenocarcinoma cells via downregualtion of p21(WAF1/CIP1)expression[J].PLo S One,2013,8(10):e77293.
[2]张冠中,焦顺昌.培美曲塞治疗非小细胞肺癌的研究进展[J].山东医药,2011,51(15):113-114.
[3]Liu JH,Chen G,Dang YW,et al.Expression and Prognostic Significance of lncRNA MALAT1 in Pancreatic Cancer Tissues[J].Asian Pac J Cancer Prev,2014,15(7):2971-2977.
[4]Emadi-Andani E,Nikpour P,Emadi-Baygi M,et al.Association of HOTAIR expression in gastric carcinoma with invasion and distant metastasis[J].Adv Biomed Res,2014,28(3):135.
[5]Rong M,He R,Dang Y,et al.Expression and clinicopathological significance of miR-146a in hepatocellular carcinoma tissues[J].Ups J Med Sci,2014,119(1):19-24.
[6]Chen G,Kronenberger P,Umelo IA,et al.Quantification of epidermal growth factor receptor T790M mutant transcripts in lung cancer cells by real-time reverse transcriptase-quantitative polymerase chain reaction[J].Anal Biochem,2010,398(2):266-268.
[7]刘敏,曾龙剑,姚树祥,等.LncRNA HOTAIR、HOTTIP及其下游HOXD10、HOXA13基因在肺癌中的表达研究[J].昆明医科大学学报,2014,35(10):49-52.
[8]林梦洁,陈志强,尹凌帝,等.长链非编码RNA HOTAIR对非小细胞肺癌迁移和侵袭能力的影响[J].临床肿瘤学杂志,2014,19(8):684-689.
[9]Liu XH,Liu ZL,Sun M,et al.The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in nonsmall cell lung cancer[J].BMC Cancer,2013,13:464.
[10]Nakaqawa T,Endo H,Yokoyama M,et al.Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer[J].Biochem Biophys Res Commun,2013,436(2):319-324.
[11]Zhao W,An Y,Liang Y,et al.Role of HOTAIR long noncoding RNA in metastatic progression of lung cancer[J].Eur Rev Med Pharmacol Sci,2014,18(13):1930-1936.
[12]Rinn JL,Kertesz M,Wang JK,et al.Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs[J].Cell,2007,129(7):1311-1323.
[13]Wan Y,Chang HY.HOTAIR:Flight of noncoding RNAs in cancer metastasis[J].Cell Cycle,2010,9(17):3391-3392.
[14]Shah N,Sukumar S.The Hox genes and their roles in oncogenesis[J].Nat Rev Cancer,2010,10(5):361-371.
[15]Li D,Feng J,Wu T,et al.Long intergenic noncoding RNA HOTAIR is overexpressed and regulates PTEN methylation in laryngeal squamous cell carcinoma[J].Am J Pathol,2013,182(1):64-70.
[16]Zeng ZY,Zhou YH,Zhang WL,et al.Gene expression profiling of nasopharyngeal carcinoma reveals the abnormally regulated Wnt signaling pathway[J].Hum Pathol,2007,38(1):120-133.
[17]Liu Z,Sun M,Lu K,et al.The long noncoding RNA HOTAIR contributes to cisplatin resistance ofhuman lung adenocarcinoma cells via downregualtion of p21(WAF1/CIP1)expression[J].PLo S One,2013,8(10):e77293.