拟南芥砷诱导基因At4g13180的表达模式研究
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摘要
拟南芥(Arabidopsis thaliana)砷诱导基因At4g13180编码蛋白是短链脱氢酶(Short-Chain Dehydrogenase/Reductase Superfamily,SDR)家族的成员之一,其过表达可以增强植物对过氧化氢的耐受性。该实验通过半定量RT-PCR,构建ProAt4g13180:GUS、At4g13180-EGFP和At4g13180-OE表达载体,获得At4g13180基因过表达转基因株系,并研究了At4g13180基因的表达模式及其编码蛋白的亚细胞定位。结果显示,At4g13180基因在根尖、叶脉、萼片和花丝等组织都强烈表达,该基因编码蛋白主要定位于胞质和核中。该研究结果为深入探究拟南芥砷诱导基因At4g13180的功能奠定了一定的基础。
In Arabidopsis thaliana,the arsenic induced gene At4g13180 encoding protein is one of the members of the SDR superfamily(Short-Chain Dehydrogenase/Reductase).Its overexpression can enhance the resistance of plants to hydrogen peroxide.Here we studied the At4g13180 gene expression pattern and the encoded protein subcellular localization by semi-quantitative RT-PCR,with transgenic plants expressing ProAt4g13180:GUS,At4g13180-EGFP and At4g13180-OE.Our results show that the At4g13180 gene has a very strong expression in tissues such as root,leaf,sepal,and stamen filamentas.At4g13180-EGFP is located in the nucleus and cytoplasm.Our study lays a basis of further research on the role of the arsenic induced gene At4g13180 in A.thaliana.
In Arabidopsis thaliana,the arsenic induced gene At4g13180 encoding protein is one of the members of the SDR superfamily(Short-Chain Dehydrogenase/Reductase).Its overexpression can enhance the resistance of plants to hydrogen peroxide.Here we studied the At4g13180 gene expression pattern and the encoded protein subcellular localization by semi-quantitative RT-PCR,with transgenic plants expressing ProAt4g13180:GUS,At4g13180-EGFP and At4g13180-OE.Our results show that the At4g13180 gene has a very strong expression in tissues such as root,leaf,sepal,and stamen filamentas.At4g13180-EGFP is located in the nucleus and cytoplasm.Our study lays a basis of further research on the role of the arsenic induced gene At4g13180 in A.thaliana.
引文
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[16]胡家恕,童富淡,邵爱萍,等.砷对大豆种子萌发的伤害[J].浙江农业大学学报,1996,22(2):121-125.HU J S,TONG F D,SHAO A P,et al.The injury of arsenic compound to the germination of soybean seeds[J].Journal of Zhejiang Agricultural University,1996,22(2):121-125.
[17]胡家恕,邵爱萍,叶兆杰,等.大豆种子萌发过程中砷毒害与膜脂过氧化作用[J].浙江农业大学学报,1995,21(4):435-440.HU J S,SHAO A P,YE Z J,et al.The injury study of arsenic compound effect on the germination of soybean seeds and peroxidation of membrane lipid[J].Journal of Zhejiang Agricultural University,1995,21(4):435-440.
[18]GERALD ZVOBGO.烟草砷吸收、亚细胞分布及形态的基因型差异与磷酸盐缓解砷毒害的机理研究[D].杭州:浙江大学,2015.
[2]郭华明,郭琦,贾永锋,等.中国不同区域高砷地下水化学特征及形成过程[J].地球科学与环境学报,2013,35(3):83-96.GUO H M,GUO Q,JIA Y F,et al.Chemical characteristics and geochemical process of high arsenic groundwater in different regions of China[J].Journal of Earth Sciences and Environment,2013,35(3):83-96.
[3]刘登义,谢建春,杨世勇,等.铜尾矿对小麦生长发育和生理功能的影响[J].应用生态学报,2001,12(1):126-128.LIU D Y,XIE J C,YANG S Y,et al.Effects of copper mine tailings on growth and development and physiological function of wheat[J].Journal of Applied Ecology,2001,12(1):126-128.
[4]ANDRE C M,LARONDELLE Y,EVERS D.Dietary Antioxidants and oxidative stress from a human and plant perspective:a review[J].Current Nutrition and Food Science,2010,6(1):2-12(11).
[5]CATARECHA P,SEGURA M D,FRANCO-ZORRILLA I M,et al.A mutant of the Arabidopsis phosphate transporter PHT1;1displays enhanced arsenic accumulation[J].Plant Cell,2007,19(3):1 123-1 133.
[6]FILLING C,BERNDT K D,BENACH J,et al.Critical residues for structure and catalysis in short-chain dehydrogenases/reductases[J].Journal of Biological Chemistry,2002,277:25 677-25 684.
[7]EKLUND H,RAMASWAMY S.Medium-and short-chain dehydrogenase/reductase gene and protein families[J].Cellular and Molecular Life Sciences Cmls,2008,65(24):3 895-3 906.
[8]COINCON M,HEITZ A,CHICHE L,et al.The betaalphabetaalphabeta elementary supersecondary structure of the Rossmann fold from porcine lactate dehydrogenase exhibits characteristics of a molten globule[J].Proteins-structure Function and Bioinformatics,2005,60(4):740-745.
[9]黄梦颖.拟南芥与核盘菌互作中调控细胞氧化还原状态的基因的鉴定与功能分析[D].福州:福建农林大学,2014.
[10]CAKMAK I,HORST W J.Effect of aluminium on lipid peroxidation,superoxide dismutase,catalase,and peroxidase activities in root tips of soybean(Glycine max)[J].Physiologia Plantaru,1991,83(3):463-468.
[11]GRAT P L,POLLE A,LEA P J,et al.Making the life of heavy metal-stressed plants a little easier[J].Functional Plant Biology,2005,32(6):481-494.
[12]STOBRAWA K,LORENC-PLUCINSKA G.Changes in antioxidant enzyme activity in the fine roots of black poplar(Populus nigra L.)and cottonwood(Populus deltoides Bartr.Ex Marsch)in heavy-metal-polluted environment[J].Plant and Soil,2007,298(1):57-68.
[13]MEHARG A A.Ecological Impact of Major Industrial Chemical Accidents[M]//Reviews of Environmental Contamination and Toxicology,USA,1994,138:21-48.
[14]ELOBEID M,GOBEL C,FEUSSNER I,et al.Cadmium interferes with auxin physiology and lignification in poplar[J].Journal of Experimental Botany.2012,63(3):1 413-1 421
[15]朱云集,王晨阳,马元喜,等.砷胁迫对小麦根系生长及活性氧代谢的影响[J].生态学报,2000,20(4):707-710.ZHU Y J,WANG C Y,MA Y X,et al.Effect of arsenic stress on the growth and metabolism of the wheat root system[J].Acta Ecologica Sinica,2000,20(4):707-710.
[16]胡家恕,童富淡,邵爱萍,等.砷对大豆种子萌发的伤害[J].浙江农业大学学报,1996,22(2):121-125.HU J S,TONG F D,SHAO A P,et al.The injury of arsenic compound to the germination of soybean seeds[J].Journal of Zhejiang Agricultural University,1996,22(2):121-125.
[17]胡家恕,邵爱萍,叶兆杰,等.大豆种子萌发过程中砷毒害与膜脂过氧化作用[J].浙江农业大学学报,1995,21(4):435-440.HU J S,SHAO A P,YE Z J,et al.The injury study of arsenic compound effect on the germination of soybean seeds and peroxidation of membrane lipid[J].Journal of Zhejiang Agricultural University,1995,21(4):435-440.
[18]GERALD ZVOBGO.烟草砷吸收、亚细胞分布及形态的基因型差异与磷酸盐缓解砷毒害的机理研究[D].杭州:浙江大学,2015.