DEPDC1基因在非肝炎病毒相关性肝细胞癌及癌旁组织中的表达及临床意义
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摘要
目的探讨DEPDC1基因与非肝炎病毒相关性肝细胞癌临床病理特征的关系及其对预后判断的意义。方法收集56例非肝炎病毒相关性肝细胞癌及癌旁组织,实时定量PCR检测癌组织及癌旁组织中DEPDC1蛋白和mRNA的表达,结合临床数据分析DEPDC1表达的意义。结果 DEPDC1蛋白在肝癌组织中的表达(7. 83±0. 17)显著高于癌旁组织(4. 67±0. 22),差异比较有统计学意义(P<0. 05); DEPDC1 mRNA在肝癌组织中的表达高于癌旁组织中的表达,高表达率为73.2%(41/56);在DEPDC1高表达组,肿瘤分化程度较低,甲胎蛋白水平较高,微血管癌栓发生率高。结论 DEPDC1分子表达水平与非肝炎病毒相关性肝癌的生物学行为相关,可能位于致癌因素最终的共同通路上。
Objective To detect the relationship between DEPDC1 molecule and clinicopathologic characteristics of non-B non-C hepatocellular carcinoma( NBNC-HCC) and its guiding significance to prognosis. Methods The tumor and the corresponding adjacent normal tissue were collected from 56 NBNC-HCC patients who underwent surgical resection. DEPDC1 expression in these specimens were assessed by real-time PCR and immunohistochemical staining. The potential significance of DEPDC1 in the prognosis of HCC were analyzed. Results Immunohistochemical staining showed positive coloration of DEPDC1 staining in HCC tissues,and the expression of HCC( 7. 83+0. 17) was significantly higher than that of adjacent tissues( 4. 67±0. 22). PCR confirmed that the high expression rate of DEPDC1 gene was 73. 2%( 41/56) in NBNC-HCC tissues. In the high expression group of DEPDC1,the tumor differentiation degree was lower,the TNM stage was more advanced,the incidence of microvascular invasion was significantly higher. Conclusion DEPDC1 expression is associated with the biological characteristics of NBNC-HCC. It may be located in the common pathways in carcinogenesis resulting in HCC.
Objective To detect the relationship between DEPDC1 molecule and clinicopathologic characteristics of non-B non-C hepatocellular carcinoma( NBNC-HCC) and its guiding significance to prognosis. Methods The tumor and the corresponding adjacent normal tissue were collected from 56 NBNC-HCC patients who underwent surgical resection. DEPDC1 expression in these specimens were assessed by real-time PCR and immunohistochemical staining. The potential significance of DEPDC1 in the prognosis of HCC were analyzed. Results Immunohistochemical staining showed positive coloration of DEPDC1 staining in HCC tissues,and the expression of HCC( 7. 83+0. 17) was significantly higher than that of adjacent tissues( 4. 67±0. 22). PCR confirmed that the high expression rate of DEPDC1 gene was 73. 2%( 41/56) in NBNC-HCC tissues. In the high expression group of DEPDC1,the tumor differentiation degree was lower,the TNM stage was more advanced,the incidence of microvascular invasion was significantly higher. Conclusion DEPDC1 expression is associated with the biological characteristics of NBNC-HCC. It may be located in the common pathways in carcinogenesis resulting in HCC.
引文
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[13] Yuan SG,Liao WJ,Yang JJ,et al.DEP domain containing1 is a novel diagnostic marker and prognostic predictor for hepatocellular carcinoma[J]. Asian Pac J Cancer Prev,2014,15(24):10917-10922.
[14] Sauzay C,Petit A,Bourgeois AM,et al.Alpha-fetoprotein(AFP):a multi-purpose marker in hepatocellular carcinoma[J].Clin Chim Acta,2016,463:39-44.
[2] Wakiyama S,Matsumoto M,Haruki K,et al.Clinical features and outcome of surgical patients with Non-B Non-C hepatocellular carcinoma[J]. Anticancer Res,2017,37(6):3207-3213.
[3] Utsunomiya T,Shimada M,Kudo M,et al.Nationwide study of 4741 patients with non-B non-C hepatocellular carcinoma with special reference to the therapeutic impact[J].Ann Surg,2014,259(2):336-345.
[4] Nishikawa H,Osaki Y.Non-B,non-C hepatocellular carcinoma(Review)[J].Int J Oncol,2013,43(5):1333-1342.
[5] Feng X,Zhang C,Zhu L,et al. DEPDC1 is required for cell cycle progression and motility in nasopharyngeal carcinoma[J].Oncotarget,2017,8(38):63605-63619.
[6] Ramalho-Carvalho J,Martins JB,Cekaite L,et al. Epigenetic disruption of miR-130a promotes prostate cancer by targeting SEC23B and DEPDC1[J]. Cancer Lett,2017,385(10):150-159.
[7]王晴晴,李爱丽,黄国锦.含DEP结构域的蛋白质1在癌症发生发展中的作用[J].广西医学,2016,38(12):1740-1743.
[8] Kaibori M,Ishizaki M,Matsui K,et al. Clinicopathologic characteristics of patients with non-B non-C hepatitis virus hepatocellular carcinoma after hepatectomy[J]. Am J Surg,2012,204(3):300-307.
[9] Kassambara A,Schoenhals M,Moreaux J,et al.Inhibition of DEPDC1A,a bad prognostic marker in multiple myeloma,delays growth and induces mature plasma cell markers in malignant plasma cells[J].Plos One,2013,8(4):e62752.
[10] Huang L,Chen K,Cai ZP,et al. DEPDC1 promotes cell proliferation and tumor growth via activation of E2F signaling in prostate cancer[J]. Biochem Biophys Res Commun,2017,490(3):707-712.
[11] Mi Y,Zhang C,Bu Y,et al.DEPDC1 is a novel cell cycle related gene that regulates mitotic progression[J]. BMB Rep,2015,48(7):413-418.
[12] Chen D,Ito S,Hyodo T,et al.Phosphorylation of DEPDC1at ser110 is required to maintain centrosome organization during mitosis[J].Exp Cell Res,2017,358(2):101-110.
[13] Yuan SG,Liao WJ,Yang JJ,et al.DEP domain containing1 is a novel diagnostic marker and prognostic predictor for hepatocellular carcinoma[J]. Asian Pac J Cancer Prev,2014,15(24):10917-10922.
[14] Sauzay C,Petit A,Bourgeois AM,et al.Alpha-fetoprotein(AFP):a multi-purpose marker in hepatocellular carcinoma[J].Clin Chim Acta,2016,463:39-44.