An In Vitro Biological Model for Evaluation of the Anti-Dandruff Performance of Hair Care Products
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摘要
An in vitro biological model for evaluation of the anti-dandruff performance of hair care products was established. In this model, Malasseziafurfur(M.furfur) was used as indicator bacteria with pigskin as substrate.This model functions in following steps: pretreatment for the substrate; inoculation of indicator bacteria;measuring the bacteriostatic efficacy by the contact plates after washing the pigskin with the sample shampoo;calculation of the effective index P; evaluation of the anti-dandruff performance by the effective index P. Three kinds of anti-dandruff agents including BPT, ZPT and OCT were tested by the model. Results showed that the order for antibacterial efficacy of the anti-dandruff agents within the mass fraction range of 0.1 %~2.0% was BPT>ZPT>OCT. Results of the evaluation on each type of shampoo showed that: one displays no antidandruff efficacy; three displays medium anti-dandruff efficacy; two displays strong anti-dandruff efficacy.
An in vitro biological model for evaluation of the anti-dandruff performance of hair care products was established. In this model, Malasseziafurfur(M.furfur) was used as indicator bacteria with pigskin as substrate.This model functions in following steps: pretreatment for the substrate; inoculation of indicator bacteria;measuring the bacteriostatic efficacy by the contact plates after washing the pigskin with the sample shampoo;calculation of the effective index P; evaluation of the anti-dandruff performance by the effective index P. Three kinds of anti-dandruff agents including BPT, ZPT and OCT were tested by the model. Results showed that the order for antibacterial efficacy of the anti-dandruff agents within the mass fraction range of 0.1 %~2.0% was BPT>ZPT>OCT. Results of the evaluation on each type of shampoo showed that: one displays no antidandruff efficacy; three displays medium anti-dandruff efficacy; two displays strong anti-dandruff efficacy.
An in vitro biological model for evaluation of the anti-dandruff performance of hair care products was established. In this model, Malasseziafurfur(M.furfur) was used as indicator bacteria with pigskin as substrate.This model functions in following steps: pretreatment for the substrate; inoculation of indicator bacteria;measuring the bacteriostatic efficacy by the contact plates after washing the pigskin with the sample shampoo;calculation of the effective index P; evaluation of the anti-dandruff performance by the effective index P. Three kinds of anti-dandruff agents including BPT, ZPT and OCT were tested by the model. Results showed that the order for antibacterial efficacy of the anti-dandruff agents within the mass fraction range of 0.1 %~2.0% was BPT>ZPT>OCT. Results of the evaluation on each type of shampoo showed that: one displays no antidandruff efficacy; three displays medium anti-dandruff efficacy; two displays strong anti-dandruff efficacy.
引文
[1] Yu Qin; Yan Min.Discussion on the evaluation of carding efficiency of hair product. China Surfactant Detergent&Cosmetics 2012, 6, 57-59.
[2] Ye Zhangzhang;Wang Ran; Xie Xiao-yuan; et al. Evaluation method on efficacy of Antidandruff cosmetics.Southern China Journal of Dermato-Venereology 2009, 16(5), 336-339.
[3] Huang Xin; Shen Liang-liang; Xu hong; et al.Inhibition of eight Chinese herbal components on M.globosa in vitro.Chin J Mycology 2011,6(2),98-100.
[4] Zheng Xiao-hui; Gao Jin; Zheng Yi; et al. Experimental study about the inhibition effects of nine kinds of traditional medicine on the isolated Malasseziastrain.Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine 2003, 2(1),16-18.
[5] ZhengXiaohui, Xia Xiujiao; Wang Zhidong; et al. Susceptibility testing of Malassezia to seven antifungal agent in vitro.Chin J Mycology 2006,20(2), 75-79.
[6] Yang Yongzhong.The Design principle of the effective chipremoving shampoo technology. China Cosmetics Review2008(7), 82-87.
[7] Lu Yonghong.Malassezia and dandruff. Southern China Journal of Dermato-Venereology 2001, 8(4), 267-269.
[8] Guo Huiyi; Zhou Xinxin; Jiang Dan. Inhibitive effect of 4 kinds of Chinese herbal and their compound on Malassezia in vitro.Chin J Mycol 2013, 8(4),210-213.
[9] Song Jie; Wen zhong; Hong Sheng-jie; et al.Research on antidandruff agents and their safety.Detergent&Cosmetics 2012,(07),20-23.
[10] Han Peng; Liu Zhiwen; Zhang Guogang; et al. Evaluation of combination therapy with vitamin E and vitamin C for idiopathic asthenospermia by Q value. The Journal of Practical Medicine 2014, 5, 738-741.
[2] Ye Zhangzhang;Wang Ran; Xie Xiao-yuan; et al. Evaluation method on efficacy of Antidandruff cosmetics.Southern China Journal of Dermato-Venereology 2009, 16(5), 336-339.
[3] Huang Xin; Shen Liang-liang; Xu hong; et al.Inhibition of eight Chinese herbal components on M.globosa in vitro.Chin J Mycology 2011,6(2),98-100.
[4] Zheng Xiao-hui; Gao Jin; Zheng Yi; et al. Experimental study about the inhibition effects of nine kinds of traditional medicine on the isolated Malasseziastrain.Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine 2003, 2(1),16-18.
[5] ZhengXiaohui, Xia Xiujiao; Wang Zhidong; et al. Susceptibility testing of Malassezia to seven antifungal agent in vitro.Chin J Mycology 2006,20(2), 75-79.
[6] Yang Yongzhong.The Design principle of the effective chipremoving shampoo technology. China Cosmetics Review2008(7), 82-87.
[7] Lu Yonghong.Malassezia and dandruff. Southern China Journal of Dermato-Venereology 2001, 8(4), 267-269.
[8] Guo Huiyi; Zhou Xinxin; Jiang Dan. Inhibitive effect of 4 kinds of Chinese herbal and their compound on Malassezia in vitro.Chin J Mycol 2013, 8(4),210-213.
[9] Song Jie; Wen zhong; Hong Sheng-jie; et al.Research on antidandruff agents and their safety.Detergent&Cosmetics 2012,(07),20-23.
[10] Han Peng; Liu Zhiwen; Zhang Guogang; et al. Evaluation of combination therapy with vitamin E and vitamin C for idiopathic asthenospermia by Q value. The Journal of Practical Medicine 2014, 5, 738-741.