高效液相色谱法同时测定陈香露白露片中3种黄酮成分的含量
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摘要
目的:建立HPLC同时测定陈香露白露片中甘草苷、芸香柚皮苷和橙皮苷含量的方法,以提高陈香露白露片的质量标准。方法:采用Phenomenex Gemini C18柱(4.6 mm×250 mm, 5μm),以乙腈-0.2%乙酸(19:81)为流动相,检测波长277 nm,体积流量为1 mL·min-1,柱温30℃。结果:甘草苷、芸香柚皮苷和橙皮苷线性范围分别为0.025 6～0.818 2μg (r=0.999 6),0.026 4～0.8448μg (r=0.9995),0.1275～4.0788μg (r=0.9996);平均回收率分别为97.78%(RSD1.58%),97.65%(RSD1.31%),98.01%(RSD 1.17%)。结论:建立的陈香露白露片含量测定方法操作简便,准确度高,提高了产品检验的工作效率,可有效控制产品质量。
OBJECTIVE To improve the quality standard of Chenxiang Lubailu tablets, detect. the contents of liquiritin,narirutin and hesperidin in the prescription simultaneously by HPLC. METHODS Phenomenex Gemini C18(4.6 mm × 250 mm, 5 μm) column was used, with the mobile phase consisting of acetonitrile and 0.2% acetic acid(19:81), the DAD-3000 detection wavelength set at 277 nm, the flow rate at 1 mL·min-1, and the column temperature at 30 ℃. RESULTSUnder the chromatographic conditions, the linear ranges of liquiritin, narirutin and hesperidin were 0.025 6-0.818 2 μg(r= 0.999 6), 0.026 4-0.844 8 μg(r = 0.999 5) and 0.127 5-4.078 8 μg(r = 0.999 6), respectively. The average recoveries were 97.78%(RSD 1.58%), 97.65%(RSD 1.31%) and 98.01%(RSD 1.17%), respectively. CONCLUSION The detection method for the components in the Chenxiang Lubailu tablets is simple, accurate, and can improve the work efficiency and,effectively control the product quality.
OBJECTIVE To improve the quality standard of Chenxiang Lubailu tablets, detect. the contents of liquiritin,narirutin and hesperidin in the prescription simultaneously by HPLC. METHODS Phenomenex Gemini C18(4.6 mm × 250 mm, 5 μm) column was used, with the mobile phase consisting of acetonitrile and 0.2% acetic acid(19:81), the DAD-3000 detection wavelength set at 277 nm, the flow rate at 1 mL·min-1, and the column temperature at 30 ℃. RESULTSUnder the chromatographic conditions, the linear ranges of liquiritin, narirutin and hesperidin were 0.025 6-0.818 2 μg(r= 0.999 6), 0.026 4-0.844 8 μg(r = 0.999 5) and 0.127 5-4.078 8 μg(r = 0.999 6), respectively. The average recoveries were 97.78%(RSD 1.58%), 97.65%(RSD 1.31%) and 98.01%(RSD 1.17%), respectively. CONCLUSION The detection method for the components in the Chenxiang Lubailu tablets is simple, accurate, and can improve the work efficiency and,effectively control the product quality.
引文
[1] Drug standard of ministry of public health of the peoples republic of China. Chinese medicine prescription preparation volume eight(中华人民共和国卫生部药品标准.中药成方制剂第八册)[S]. 1993:87-88.
[2] Zhou J, Li JL, Fu YH, et al. Quality control study on Chinese medicine composition of Chenxiang Lubailu tablets[J]. Chin Hosp Pharm J(中国医院药学杂志),2006, 26(10):1314-1316.
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[5] Li YF, Li ZH, Zhu ZH. HPLC determination of glycyrrhizic acid in Chenxianglubailu tablets[J]. Chin J Pharm Anal(药物分析杂志),2008, 28(2):304-305.
[6] Zhang BX, Zhou FQ, Guo QM. Research progress of flavonoids on Lonicerae japonicae Flos in last five years[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2012,18(23):349-352.
[7] Zhan TX, Pan H, Xie H, et al. The treatment and its mechanism of hesperidin on the ulcerative colitis in rats[J]. Acta Acad Med Weifang(潍坊医学院学报),2011,33(2):160.
[8] Funaguchi N, Ohno Y, La BL, et al. Narirutin inhibits airway inflammation in an allergic mouse model[J]. Clin Exp Pharmacol Physiol, 2007, 34(8):766-770.
[9] Ha SK, Park HY, Eom H, et al. Narirutin fraction from citrus peels attenuates LPS-stimulated inflammatory response through inhibition of NF-KB and MAPKs activation[J]. Food Chem Toxicol, 2012, 50(10):3498-3504.
[10] Li RWS, Leung GPH. Vasodilatory effect of narirutin in rat mesenteric arteries and aortae[J]. J Vase Res, 2013, 50(2):37.
[11] Chakraborty S, Basu S. Multi-functional activities of citrus flavonoid narirutin in Alzheimer's disease therapeutics:An integrated screening approach and in vitro validation[J]. Int J Biol Macromol, 2017, 103:733-743.
[12] Park HY, Ha SK, Eom H, et al. Narirutin fraction from citrus peels attenuates alcoholic liver disease in mice[J].Food Chem Toxicol, 2013, 55(3):637-644.
[13] Tan SS, Chen HF, Luo XQ, et al. Effects of the narirutin combined with hesperidin in Fructus Aurantii on small intestinal propulsion function of mice[J]. J Jiangxi Univ Tradit Chin Med(江西中医药大学学报),2017, 29(4):73-75.
[14] Li CS,Pan SY. The application of liquiritin in pharmaceutical industry:China, 200610016770.O[P]. 2007-09-12.
[15]中国药典.一部(Ch. P. VolⅠ)[S]. 2015:191.
[2] Zhou J, Li JL, Fu YH, et al. Quality control study on Chinese medicine composition of Chenxiang Lubailu tablets[J]. Chin Hosp Pharm J(中国医院药学杂志),2006, 26(10):1314-1316.
[3] Tong K, Ma J,Sun H, et al. Quality standard for Chenxianglubailu tablet[J]. Cent South Pharm(中南药学),2012, 10(9):644-648.
[4] Peng ZX, Zhang YJ, Xiong RY. Determination of glycyrrhetic acid in Chenxianglubailu tablets by HPLC[J].Mod Chin Med(中国现代中药),2007, 9(9):18-20.
[5] Li YF, Li ZH, Zhu ZH. HPLC determination of glycyrrhizic acid in Chenxianglubailu tablets[J]. Chin J Pharm Anal(药物分析杂志),2008, 28(2):304-305.
[6] Zhang BX, Zhou FQ, Guo QM. Research progress of flavonoids on Lonicerae japonicae Flos in last five years[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2012,18(23):349-352.
[7] Zhan TX, Pan H, Xie H, et al. The treatment and its mechanism of hesperidin on the ulcerative colitis in rats[J]. Acta Acad Med Weifang(潍坊医学院学报),2011,33(2):160.
[8] Funaguchi N, Ohno Y, La BL, et al. Narirutin inhibits airway inflammation in an allergic mouse model[J]. Clin Exp Pharmacol Physiol, 2007, 34(8):766-770.
[9] Ha SK, Park HY, Eom H, et al. Narirutin fraction from citrus peels attenuates LPS-stimulated inflammatory response through inhibition of NF-KB and MAPKs activation[J]. Food Chem Toxicol, 2012, 50(10):3498-3504.
[10] Li RWS, Leung GPH. Vasodilatory effect of narirutin in rat mesenteric arteries and aortae[J]. J Vase Res, 2013, 50(2):37.
[11] Chakraborty S, Basu S. Multi-functional activities of citrus flavonoid narirutin in Alzheimer's disease therapeutics:An integrated screening approach and in vitro validation[J]. Int J Biol Macromol, 2017, 103:733-743.
[12] Park HY, Ha SK, Eom H, et al. Narirutin fraction from citrus peels attenuates alcoholic liver disease in mice[J].Food Chem Toxicol, 2013, 55(3):637-644.
[13] Tan SS, Chen HF, Luo XQ, et al. Effects of the narirutin combined with hesperidin in Fructus Aurantii on small intestinal propulsion function of mice[J]. J Jiangxi Univ Tradit Chin Med(江西中医药大学学报),2017, 29(4):73-75.
[14] Li CS,Pan SY. The application of liquiritin in pharmaceutical industry:China, 200610016770.O[P]. 2007-09-12.
[15]中国药典.一部(Ch. P. VolⅠ)[S]. 2015:191.