ABCA1对滋养细胞基因表达谱的影响

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英文篇名：Effect of ABCA1 on Gene Expression Profiles of Trophoblast Cells 作者：杜海霞 ; 谢成茂 ; 张晶晶 ; 蔺莉 英文作者：Du Haixia;Xie Chengmao;Zhang Jingjing;Lin li;Department of Gynaecology, Beijing Obstetrics and Gynecology Hospital of Capital Medical University;Department of Delivery Room, Beijing Obstetrics and Gynecology Hospital of Capital Medical University;Department of Obstetrics and Gynecology, Perking University International Hospital; 关键词：ABCA1 ; 滋养细胞 ; 基因表达谱 ; 信号通路 英文关键词：ABCA1;;trophoblast cells;;gene expression profile;;signal pathways 中文刊名：XBZZ 英文刊名：Chinese Journal of Cell Biology 机构：首都医科大学附属北京妇产医院妇科;首都医科大学附属北京妇产医院分娩室;北京大学国际医院妇产科; 出版日期：2019-05-10 16:42 出版单位：中国细胞生物学学报 年：2019 期：v.41 基金：国家自然科学基金(批准号:81471476)资助的课题~~ 语种：中文; 页：XBZZ201904010 页数：8 CN：04 ISSN：31-2035/Q 分类号：81-88

摘要

该文从基因学角度探讨了ABCA1对人滋养细胞功能的影响。在人滋养细胞中调控ABCA1的表达,通过表达谱芯片检测ABCA1表达改变后滋养细胞中基因及相关信号通路的改变,Western blot及qRT-PCR验证芯片结果。结果显示,上调ABCA1表达时,有197个基因表达升高,有190个基因表达下降;而下调ABCA1表达时,有335个基因表达升高, 459个基因表达下降。GO和KEGG分析表明, ABCA1表达上升或下降后可导致滋养细胞内多个信号通路发生改变。qRT-PCR及Western blot检测发现,与阴性对照组相比, ABCA1上调后细胞中S1PR1的m RNA及蛋白水平明显升高,而CCL8、CXCL10与CXCL11的mRNA及蛋白水平明显降低;下调ABCA1的表达后,细胞中S1PR1的mRNA及蛋白水平明显降低,而CCL8、CXCL10与CXCL11的mRNA及蛋白水平明显升高,与芯片的结果完全一致。这些结果表明, ABCA1可通过调控多个信号通路而影响滋养细胞功能。
        This artical explored the effect of ABCA1 on human trophoblast function from the genetic perspective. The expression of ABCA1 in human trophoblast cells was regulated, and the changes of genes and related signaling pathways in trophoblast cells were detected by expression microarray, and the results were verified by Western blot and qRT-PCR. The results showed that when ABCA1 expression was up-regulated, 197 genes were up-regulated and 190 genes were down-regulated. When ABCA1 expression was down-regulated, 335 genes were elevated in expression and 459 genes were down-regulated. GO and KEGG analysis indicate that increased or decreased ABCA1 expression leads to changes in multiple signaling pathways in trophoblasts. Compared with the negative control group, the mRNA and protein levels of S1 PR1 in the cells up-regulated by ABCA1 were significantly increased, while the mRNA and protein levels of CCL8, CXCL10 and CXCL11 were significantly decreased. The expression of ABCA1 was down-regulated after qRT-PCR and Western blot assay. The mRNA and protein levels of S1 PR1 in the cells were significantly decreased, while the mRNA and protein levels of CCL8,CXCL10 and CXCL11 were significantly increased, which was consistent with the results of the chip. These results indicate that ABCA1 can affect trophoblast function by regulating multiple signaling pathways.

引文

1 Luciani MF,Chimini G.The ATP binding cassette transporter ABC1,is required for the engulfment of corpses generated by apoptotic cell death.EMBO J 1996;15(2):226-35.
    2 Liu L,Zhang M,Li L,Li C,Min X,Zheng M.Expression and clinical significance of ATP-binding cassette transporter 1 in serum and placental tissue in Chinese patients with preeclampsia.Gynecol Obstet Invest 2014;78(3):194-200.
    3 Borbely AU,Sandri S,Fernandes IR,Prado KM,Cardoso EC,Correa-Silva S,et al.The term basal plate of the human placenta as a source of functional extravillous trophoblast cells.Reprod Biol Endocrinol 2014;12:7.
    4 Aye IL,Waddell BJ,Mark PJ,Keelan JA.Placental ABCA1 and ABCG1 transporters efflux cholesterol and protect trophoblasts from oxysterol induced toxicity.Biochim Biophys Acta 2010;1801(9):1013-24.
    5 Cui X,Churchill GA.Statistical tests for differential expression in cDNA microarray experiments.Genome Biol 2003;4(4):210.
    6 Genbacev OD,Prakobphol A,Foulk RA,Krtolica AR,Ilic D,Singer MS,et al.Trophoblast L-selectin-mediated adhesion at the maternal-fetal interface.Science 2003;299(5605):405-8.
    7 Aplin JD.Developmental cell biology of human villous trophoblast:current research problems.Int J Dev Biol 2010;54(2/3):323-9.
    8 Waeber C,Walther T.Sphingosine-1-phosphate as a potential target for the treatment of myocardial infarction.Circ J 2014;78(4):795-802.
    9 Chen W,Zheng R,Baade PD,Zhang S,Zeng H,Bray F,et al.Cancer statistics in China,2015.CA Cancer J Clin 2016;66(2):115-32.
    10 Palomino DC,Marti LC.Chemokines and immunity.Einstein(Sao Paulo)2015;13(3):469-73.
    11 Bosisio D,Salvi V,Gagliostro V,Sozzani S.Angiogenic and antiangiogenic chemokines.Chem Immunol Allergy 2014;99:89-104.
    12 Vacchini A,Locati M,Borroni EM.Overview and potential unifying themes of the atypical chemokine receptor family.JLeukoc Biol 2016;99(6):883-92.
    13 Bryant VL,Slade CA.Chemokines,their receptors and human disease:the good,the bad and the itchy.Immunol Cell Biol 2015;93(4):364-71.
    14 Roy I,Evans DB,Dwinell MB.Chemokines and chemokine receptors:update on utility and challenges for the clinician.Surgery 2014;155(6):961-73.