文冠果果实转录组测序及分析
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摘要
为了研究文冠果果实转录组中功能基因表达情况,采样RNA-seq技术对文冠果不同发育时期果实进行测序分析。结果显示:两个时期样品材料测序组装后共获得68 298个Unigene序列,有24 691个Unigene得到注释,占36.15%;GO数据库注释显示14 766条Unigene分为细胞组分、分子功能及生物学过程3大类54个功能组;KOG数据库中注释到的13 994条Unigene功能系统分为25类;以KEGG代谢途径数据库为依据,可将8 284个文冠果果实转录组Unigene分为127个代谢通路;在文冠果果实转录组中发现11 732个SSR位点,其中最多的为单核苷酸SSR,占60.85%。本研究为文冠果果实分子生物学研究提供了一定的基础和参考。
In order to study the expressions of functional genes in the transcriptome of Xanthoceras sorbifolia Bunge fruit,the RNA-Seq technology was used to sequence Xanthoceras sorbifolia Bunge fruit in different stag. The sequencing results showed that a total of 68 298 Unigene sequences after sequencing and assembly were obtained from the samples in 2 stages,and 24 691 Unigene sequences were annotated,accounting for 36.15%. Based on GO database,the 14 766 Unigene sequences were divided into 3 categories of cellular component,molecular function and biological process and 54 function groups. The 13 994 Unigene sequences were grouped into 25 functional categories based on KOG database. Based on KEGG metabolic pathway database,8 284 Unigene sequences of X. sorbifolia Bunge fruit may be classified into 127 metabolic pathways. And 11 732 SSR(Simple Sequence Repeats)loci were discovered in X. sorbifolia Bunge fruit transcriptome. Most of them were single nucleotide SSR,accounting for 60.85%. This study provides the foundation and reference for molecular biology study in X.sorbifolia Bunge fruit.
In order to study the expressions of functional genes in the transcriptome of Xanthoceras sorbifolia Bunge fruit,the RNA-Seq technology was used to sequence Xanthoceras sorbifolia Bunge fruit in different stag. The sequencing results showed that a total of 68 298 Unigene sequences after sequencing and assembly were obtained from the samples in 2 stages,and 24 691 Unigene sequences were annotated,accounting for 36.15%. Based on GO database,the 14 766 Unigene sequences were divided into 3 categories of cellular component,molecular function and biological process and 54 function groups. The 13 994 Unigene sequences were grouped into 25 functional categories based on KOG database. Based on KEGG metabolic pathway database,8 284 Unigene sequences of X. sorbifolia Bunge fruit may be classified into 127 metabolic pathways. And 11 732 SSR(Simple Sequence Repeats)loci were discovered in X. sorbifolia Bunge fruit transcriptome. Most of them were single nucleotide SSR,accounting for 60.85%. This study provides the foundation and reference for molecular biology study in X.sorbifolia Bunge fruit.
引文
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[15]刘晓辉,马静怡,罗媛,等.木本油料文冠果APETALA2基因全长cDNA序列与生物信息学分析[J].现代农业科技,2018(2):145-146.
[16]路蒙蒙,韩硕,杨琦,等.文冠果LEC1基因的克隆及表达分析[J].北京林业大学学报, 2018(1):8-16.
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[18]赵娜,张媛,李秋琦,等.文冠果FAD2的序列与功能分析[J].北京林业大学学报, 2015, 37(2):000087-93.
[19]Guo HH, Wang TT, Li QQ, et al. Two novel diacylglycerol acyltransferase genes from Xanthoceras sorbifolia are responsible for its seed oil content[J]. Gene, 2013, 527(1):266-274.
[20]李和平,姚运法,练冬梅,等.黄秋葵果实转录组测序及分析[J].生物技术通报, 2018, 34(3):121-127.
[21]Liu Y, Huang Z, Ao Y, et al. Transcriptome analysis of yellow horn(Xanthoceras sorbifolia Bunge):a potential oil-rich seed tree for biodiesel in China[J]. PLoS One, 2014, 9(1):e74441.
[22]敖妍,马履一,韩树文,等.基于高通量测序的文冠果转录组分析[J].中国生物工程杂志, 2015, 35(7):22-29.
[23]刘玉林,李伟,董树斌,等.文冠果转录组SSR特征分析及EST-SSR标记开发[J].西北农林科技大学学报:自然科学版,2017, 45(3):89-95.
[24]申展,马履一,等.基于SSR标记的文冠果遗传多样性分析及核心种质构建[J].分子植物育种, 2017(8):3341-3350.
[25]敖妍.文冠果种子产量影响因素分析[J].中国农学通报,2008, 24(8):300-304.
[26]敖妍,马履一.文冠果分子生物学研究进展[J].分子植物育种, 2016(12):3570-3576.
[27]王涛.中国能源植物文冠果的研究[M].北京:中国科学技术出版社, 2012.
[28]金亮,田丹青,詹书侠,等.松萝凤梨转录组测序分析[J].分子植物育种, 2018:1-13.
[29]李依民,张化为,陈莹,等.白鲜根转录组高通量测序与数据分析[J].中草药, 2018, 49(21):4975-4982.
[2]敖妍,段劼,于海燕,等.文冠果研究进展[J].中国农业大学学报, 2012, 17(6):197-203.
[3]孔维宝,梁俊玉,马正学,等.文冠果油的研究进展[J].中国油脂, 2011, 36(11):67-72.
[4]赵翠格.文冠果种子发育过程中油脂累积规律研究[D].北京:北京林业大学, 2010.
[5]赵娜,张媛,等.文冠果种子发育及油脂累积与糖类、蛋白质累积之间的关系研究[J].植物研究, 2015, 35(1):133-140.
[6]赵翠格,刘頔,李凤兰,等.植物种子油脂的生物合成及代谢基础研究进展[J].种子, 2010, 29(4):56-62.
[7]苏曼琳,吴尚,马履一,等. 3种修剪方法对文冠果芽内源激素含量和萌芽成枝的影响[J].西北农林科技大学学报:自然科学版, 2017, 45(5):101-108.
[8]史绍林.文冠果种子催芽和组织培养研究[D].齐齐哈尔:齐齐哈尔大学, 2012.
[9]邓红,何玲,孙俊.文冠果油的冷榨提取及理化性质研究[J].西南大学学报:自然科学版, 2006, 28(6):1027-1031.
[10]付玉杰,张乃静,王黎丽,等.超声提取文冠果种仁油及GC-MS成分分析[J].植物研究, 2007, 27(5):622-625.
[11]王黎丽,付玉杰,张谡.文冠果种子油提取工艺[J].植物研究,2008, 28(4):509-512.
[12]郝一男,王喜明,丁立军.文冠果油生物柴油副产物粗甘油的精制[J].中国油脂, 2011, 36(11):80-82.
[13]孙俊.文冠果油的提取及其生物柴油制备工艺研究[D].陕西师范大学, 2008.
[14]戚建华,姚增玉.文冠果的生殖生物学与良种繁育研究进展[J].西北林学院学报, 2012, 27(3):91-96.
[15]刘晓辉,马静怡,罗媛,等.木本油料文冠果APETALA2基因全长cDNA序列与生物信息学分析[J].现代农业科技,2018(2):145-146.
[16]路蒙蒙,韩硕,杨琦,等.文冠果LEC1基因的克隆及表达分析[J].北京林业大学学报, 2018(1):8-16.
[17]范春霞.文冠果雄花性别决定中雌蕊败育相关基因的克隆及初步鉴定[D].北京:北京林业大学, 2009.
[18]赵娜,张媛,李秋琦,等.文冠果FAD2的序列与功能分析[J].北京林业大学学报, 2015, 37(2):000087-93.
[19]Guo HH, Wang TT, Li QQ, et al. Two novel diacylglycerol acyltransferase genes from Xanthoceras sorbifolia are responsible for its seed oil content[J]. Gene, 2013, 527(1):266-274.
[20]李和平,姚运法,练冬梅,等.黄秋葵果实转录组测序及分析[J].生物技术通报, 2018, 34(3):121-127.
[21]Liu Y, Huang Z, Ao Y, et al. Transcriptome analysis of yellow horn(Xanthoceras sorbifolia Bunge):a potential oil-rich seed tree for biodiesel in China[J]. PLoS One, 2014, 9(1):e74441.
[22]敖妍,马履一,韩树文,等.基于高通量测序的文冠果转录组分析[J].中国生物工程杂志, 2015, 35(7):22-29.
[23]刘玉林,李伟,董树斌,等.文冠果转录组SSR特征分析及EST-SSR标记开发[J].西北农林科技大学学报:自然科学版,2017, 45(3):89-95.
[24]申展,马履一,等.基于SSR标记的文冠果遗传多样性分析及核心种质构建[J].分子植物育种, 2017(8):3341-3350.
[25]敖妍.文冠果种子产量影响因素分析[J].中国农学通报,2008, 24(8):300-304.
[26]敖妍,马履一.文冠果分子生物学研究进展[J].分子植物育种, 2016(12):3570-3576.
[27]王涛.中国能源植物文冠果的研究[M].北京:中国科学技术出版社, 2012.
[28]金亮,田丹青,詹书侠,等.松萝凤梨转录组测序分析[J].分子植物育种, 2018:1-13.
[29]李依民,张化为,陈莹,等.白鲜根转录组高通量测序与数据分析[J].中草药, 2018, 49(21):4975-4982.