莲原花青素与金属离子配合率的测定
|
摘要
分别采用紫外分光光度计法、滴定法、电化学法研究了莲原花青素对金属离子的配合物配合率值。结果表明,3种方法测量配合率值时各有利弊,并分别找出3种方法适用的莲原花青素与金属离子配合率测定的不同浓度范围。其中极谱法适用于(1~10)mg/kg莲原花青素,紫外分光光度计法适用于(10~100)mg/kg莲原花青素,滴定法适用于较高浓度莲原花青素(1000 mg/kg)与金属离子配合率的测定。在实验过程中还可以得出p H是影响配合物形成的重要因素。Fe~(3+)、Fe~(2+)、Cu~(2+)、Pb~(2+)离子在酸性条件下即可与莲原花青素配合,而Zn~(2+)、Mn~(2+)、Ca~(2+)在溶液p H更高时才能与莲原花青素配合。
The lotus seedpod procyanidins with metal ions complex rates were studied by ultraviolet spectrophotometer method, titration method, electrochemical method. The results showed that the three methods had advantages and disadvantages of each measuring cooperate rate values, and it found out three ways to apply to different concentration ranges for the determination of the combination of lotus seedpod proanthocyanidins and metal ions. Polarography was suitable for(1~10)mg/kg lotus seedpod procyanidins, ultraviolet spectrophotometer method was suitable for(10~100)mg/kg lotus seedpod procyanidins, titration method was suitable for the relatively high concentrations of lotus seedpod procyanidins(1000 mg/kg) cooperate with metal ion rates determination. In the process of experiment, it could be concluded that p H was an important factor affecting the formation of complexes. Fe~(3+), Fe~(2+), Cu~(2+), Pb~(2+) ions could cooperate with lotus seedpod procyanidins under acid condition, and Zn~(2+), Mn~(2+), Ca~(2+) in higher p H solution could cooperate with lotus seedpod procyanidins.
The lotus seedpod procyanidins with metal ions complex rates were studied by ultraviolet spectrophotometer method, titration method, electrochemical method. The results showed that the three methods had advantages and disadvantages of each measuring cooperate rate values, and it found out three ways to apply to different concentration ranges for the determination of the combination of lotus seedpod proanthocyanidins and metal ions. Polarography was suitable for(1~10)mg/kg lotus seedpod procyanidins, ultraviolet spectrophotometer method was suitable for(10~100)mg/kg lotus seedpod procyanidins, titration method was suitable for the relatively high concentrations of lotus seedpod procyanidins(1000 mg/kg) cooperate with metal ion rates determination. In the process of experiment, it could be concluded that p H was an important factor affecting the formation of complexes. Fe~(3+), Fe~(2+), Cu~(2+), Pb~(2+) ions could cooperate with lotus seedpod procyanidins under acid condition, and Zn~(2+), Mn~(2+), Ca~(2+) in higher p H solution could cooperate with lotus seedpod procyanidins.
引文
[1]郑文杰,黄宁兴.配位化学在药学研究中的应用(综述)[J].暨南大学学报(自然科学版),1993,14(3):68-73
[2]李佳桥,余修亮,曾林晖,等.响应面试验优化超声波辅助提取莲房原花青素工艺[J].食品科学,2016,37(12):40-45
[3]汪志慧,孙智达,谢笔钧.响应曲面法优化双酶法提取莲房原花青素[J].食品科学,2011,32(4):64-68
[4]禹华娟,孙智达,谢笔钧.酶辅助提取莲房原花青素工艺及其抗氧化活性研究[J].天然产物研究与开发,2010,(1)
[5]段玉清,张海晖,李金凤,等.脉冲超声辅助提取莲房多酚的工艺[J].农业工程学报,2009,24(3):193-197
[6]段玉清,闫永胜,张海晖,等.莲房多酚的微波辅助提取技术[J].江苏大学学报(自然科学版),2009,(5):437-440
[7]陈卫航,谭美亭,张婕.响应面法优化莲房原花青素提取工艺研究[J].郑州大学学报,2012,30(2):31-35
[8]Osawa H,Endo I,Hara Y,et al.Transient proliferation of proanthocyanidin-accumulating cells on the epidermal apex contributes to highly aluminumresistant root elongation in camphor tree[J].Plant physiology,2011,155(1):433-446
[9]ESPARZA I,SALINAS I,SANTAMARIA C,et al.Electrochemical and theoretical complexation studies for Zn and Cu with individual polyphenols[J].Analytica Chimica Acta,2005,543(1):267-274
[10]BAI Y,SONG F,CHEN M,et al.Characterization of the Rutin-Metal Complex by Electrospray Ionization Tandem Mass Spectrometry[J].Analytical Sciences August,2004,20(8):1147-1151
[11]陈貌连,宋凤瑞,刘志强,等.槲皮素和芦丁的某些金属配合物的电喷雾质谱研究[J].分析测试学报,2001,20(Z1):101-102
[12]郭琛,黄启亮,徐丽珊.温度、p H及常见金属离子对松针原花青素稳定性影响初探[J].中国科技纵横,2010,(2):28-29
[13]OHYOSHI E,SAKATA T,KURIHARA M.Complexation of al-uminium with(-)-epigallocathechin gallate studied by spectrophotometry[J].Journal of Inorganic Biochemistry,1999,73(1):31-34
[14]MIRA L,FERNANDEZ M,SANTOS M,et al.Interactions of flavonoids with iron and copper ions:a mechanism for their antioxidant activity[J].Free radical research,2002,36(11):1199-1208
[15]HYNES M J,COINCEANAINN M O.The kinetics and mechanisms of the reaction of iron(III)with gallic acid,gallic acid methyl ester and catechin[J].Journal of Inorganic Biochemistry,2001,85(2):131-142
[16]VAN ACKER S A B E,VAN BALE G P,VAN DEN BERG D J,et a1.Influence of iron chelation on the antioxidant activity of flavonoids[J].Biochemical Pharmacology,1998,56(8):935-943
[17]BROWN E J,Khodr H,HIDER C R,et al.Structural dependence of flavonoid interactions with Cu2+ions:implications for their antioxidant properties[J].Biochemical Journal,1998,330(3):1173-1178
[18]韩志萍,卢翠英.示波极谱法测定葡萄籽提取物中原青素[J].理化检验-化学分册,2005,41(4):245-247
[19]HAYAKAWA F,KIMURA T,HOSHINO N,et al.DNA cleavage activities of(-)-epigallocatechin,(-)-epicatechin,(+)-catechin,and(-)-epigallocatechin gallate with various kind of metal ions[J].Bioscience,biotechnology,and biochemistry,1999,63(9):1654-1656
[20]KHOKHAR S,APENTEN R K O.Ironbinding characteristics of phenolic compounds:some tentative structure-activity relations[J].Food Chemistry,2003,81(1):133-140
[2]李佳桥,余修亮,曾林晖,等.响应面试验优化超声波辅助提取莲房原花青素工艺[J].食品科学,2016,37(12):40-45
[3]汪志慧,孙智达,谢笔钧.响应曲面法优化双酶法提取莲房原花青素[J].食品科学,2011,32(4):64-68
[4]禹华娟,孙智达,谢笔钧.酶辅助提取莲房原花青素工艺及其抗氧化活性研究[J].天然产物研究与开发,2010,(1)
[5]段玉清,张海晖,李金凤,等.脉冲超声辅助提取莲房多酚的工艺[J].农业工程学报,2009,24(3):193-197
[6]段玉清,闫永胜,张海晖,等.莲房多酚的微波辅助提取技术[J].江苏大学学报(自然科学版),2009,(5):437-440
[7]陈卫航,谭美亭,张婕.响应面法优化莲房原花青素提取工艺研究[J].郑州大学学报,2012,30(2):31-35
[8]Osawa H,Endo I,Hara Y,et al.Transient proliferation of proanthocyanidin-accumulating cells on the epidermal apex contributes to highly aluminumresistant root elongation in camphor tree[J].Plant physiology,2011,155(1):433-446
[9]ESPARZA I,SALINAS I,SANTAMARIA C,et al.Electrochemical and theoretical complexation studies for Zn and Cu with individual polyphenols[J].Analytica Chimica Acta,2005,543(1):267-274
[10]BAI Y,SONG F,CHEN M,et al.Characterization of the Rutin-Metal Complex by Electrospray Ionization Tandem Mass Spectrometry[J].Analytical Sciences August,2004,20(8):1147-1151
[11]陈貌连,宋凤瑞,刘志强,等.槲皮素和芦丁的某些金属配合物的电喷雾质谱研究[J].分析测试学报,2001,20(Z1):101-102
[12]郭琛,黄启亮,徐丽珊.温度、p H及常见金属离子对松针原花青素稳定性影响初探[J].中国科技纵横,2010,(2):28-29
[13]OHYOSHI E,SAKATA T,KURIHARA M.Complexation of al-uminium with(-)-epigallocathechin gallate studied by spectrophotometry[J].Journal of Inorganic Biochemistry,1999,73(1):31-34
[14]MIRA L,FERNANDEZ M,SANTOS M,et al.Interactions of flavonoids with iron and copper ions:a mechanism for their antioxidant activity[J].Free radical research,2002,36(11):1199-1208
[15]HYNES M J,COINCEANAINN M O.The kinetics and mechanisms of the reaction of iron(III)with gallic acid,gallic acid methyl ester and catechin[J].Journal of Inorganic Biochemistry,2001,85(2):131-142
[16]VAN ACKER S A B E,VAN BALE G P,VAN DEN BERG D J,et a1.Influence of iron chelation on the antioxidant activity of flavonoids[J].Biochemical Pharmacology,1998,56(8):935-943
[17]BROWN E J,Khodr H,HIDER C R,et al.Structural dependence of flavonoid interactions with Cu2+ions:implications for their antioxidant properties[J].Biochemical Journal,1998,330(3):1173-1178
[18]韩志萍,卢翠英.示波极谱法测定葡萄籽提取物中原青素[J].理化检验-化学分册,2005,41(4):245-247
[19]HAYAKAWA F,KIMURA T,HOSHINO N,et al.DNA cleavage activities of(-)-epigallocatechin,(-)-epicatechin,(+)-catechin,and(-)-epigallocatechin gallate with various kind of metal ions[J].Bioscience,biotechnology,and biochemistry,1999,63(9):1654-1656
[20]KHOKHAR S,APENTEN R K O.Ironbinding characteristics of phenolic compounds:some tentative structure-activity relations[J].Food Chemistry,2003,81(1):133-140
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |