矮凤梨短营养生长期突变材料‘14-1’的性状鉴定
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摘要
菠萝栽培种(Ananas comosus var. comosus)的营养生长期较长,制约了其新品种选育效率。收集和筛选营养生长期较短的种质,可为短营养生长期菠萝新品种的选育创制中间材料。从境外收集了23份易成花的野生种质材料,自2008年起进行栽培、繁殖和性状鉴定。通过对营养生长期的比较和观察,从矮凤梨(A. comosus var. nanus)的体胚苗中发现了1份营养生长期显著缩短的突变材料‘14-1’,其营养生长期约6个月(原品种约12个月),1年开花2次,具有极易成花等特点。‘14-1’无性繁殖后代遗传性状稳定,可作为短营养生长期菠萝育种的亲本资源。
Pineapple(Ananas comosus var. comosus)has a long vegetative growth period,restricting the efficiency of its new cultivar breeding. Collection and selection of germplasm with a short vegetative growth period can create intermediate materials for new short-vegetative-growth-period cultivars of pineapple. We have collected 23 wild germplasm materials from abroad,which are easy to flower. Since 2008,the introduced germplasms have been cultivated,propagated and character identified. Through comparing and observing the vegetative growth period,a short-vegetative-growth-period mutant ‘14-1' was discovered,from the somatic embryogenesis seedlings of Ananas comosus var. nanus. This mutant is extremely easy to flower. Compared with the original variety,the mutant has a vegetative growth period as long as about 6 months(original variety is about 12 months),i.e.,it blooms twice a year. The characterization of mutant ‘14-1' can be stably inherited in its cloned plants,so it is a scarce parental material for short-vegetative-growth-period pineapple breeding.
Pineapple(Ananas comosus var. comosus)has a long vegetative growth period,restricting the efficiency of its new cultivar breeding. Collection and selection of germplasm with a short vegetative growth period can create intermediate materials for new short-vegetative-growth-period cultivars of pineapple. We have collected 23 wild germplasm materials from abroad,which are easy to flower. Since 2008,the introduced germplasms have been cultivated,propagated and character identified. Through comparing and observing the vegetative growth period,a short-vegetative-growth-period mutant ‘14-1' was discovered,from the somatic embryogenesis seedlings of Ananas comosus var. nanus. This mutant is extremely easy to flower. Compared with the original variety,the mutant has a vegetative growth period as long as about 6 months(original variety is about 12 months),i.e.,it blooms twice a year. The characterization of mutant ‘14-1' can be stably inherited in its cloned plants,so it is a scarce parental material for short-vegetative-growth-period pineapple breeding.
引文
Chan Y K.2008.Breeding of seed and vegetatively propagated tropical fruits using papaya and pineapple as examples.Acta Horticulturae,787:69-76.
D’Eeckenbrugge C.1993.Utilization of pineapple genetic resources in breeding reports.Tropical Fruits Newsletter,IICA-PRRET A2/TT:94-101.
Fan Shuang-xi,Li Guang-chen.2007.Horticultural plant cultivation.Beijign:China Agricultural University Press:21-32.(in Chinese)范双喜,李光晨.2007.园艺植物栽培学.北京:中国农业大学出版社:21-32.
Gong Xue.2016.Investigation on the main characters of pineapple in‘Shenwan’and selection of superior plants[M.D.Dissertation].Guangzhou:South China Agricultural University.(in Chinese)龚雪.2016.神湾菠萝主要性状调查与优株选择[硕士论文].广州:华南农业大学.
Haque S M,Ghosh B.2017.Regeneration of cytologically stable plants through dedifferentiation,redifferentiation,and artificial seeds in Spathoglottis plicata Blume.(Orchidaceae).Horticultural Plant Journal,3(5):199-208.
He Ye-hua,Fang Shao-qiu,Ma Jun,Hu Zhong-yi,Lu Min,Peng Bing.2010.Histocytology observation on the somatic embryogenesis in Ananas comosus callus.Acta Horticulturae Sinica,37(5):689-696.(in Chinese)何业华,方少秋,马均,胡中沂,卢敏,彭兵.2010.菠萝愈伤组织中体细胞胚起源过程的组织细胞学观察.园艺学报,37(5):689-696.
He Ye-hua,Hu Zhong-yi,Ma Jun,Fang Shao-qiu,Lu Min,Xie Zhi-liang,Peng Bing.2009.Germplasm resources and taxonomy of bromeliad.Nonwood Forest Research,27(3):102-107.(in Chinese)何业华,胡中沂,马均,方少秋,卢敏,谢志亮,彭兵.2009.凤梨类植物的种质资源与分类.经济林研究,27(3):102-107.
He Ye-hua,Luo Ji,Wu Hui-tao,Wang Rui-xia,Gao Ai-ping,Zhao Chun-xiang,Yu Xiao-ling,Ye Zi-xing,Wang Ze-huai,Han Jing-zhong,Liu He-ping.2007.Somatic embryogenesis from leaf base callus of Ananas comosus.Journal of Fruit Science,24(1):59-63.(in Chinese)何业华,罗吉,吴会桃,王瑞霞,高爱平,赵春香,余小玲,叶自行,王泽槐,韩景忠,刘和平.2007.菠萝叶基愈伤组织诱导体细胞胚.果树学报,24(1):59-63.
He Ye-hua,Zhang Ya-fen,Xia Jing-xian,Guo Cui-hong,Cao Li,Zhang Jun-li.2014.Screening of pineapple cold resistant cell lines.Acta Horticulturae Sinica,41(Suppl):2654.(in Chinese)何业华,张雅芬,夏靖娴,郭翠红,曹莉,张俊丽.2014.菠萝抗寒细胞株系的筛选.园艺学报,41(Suppl):2654.
Huang Long-jun.2004.Study on improving bolting rate of pineapple.Fruit Trees in Southern China,33(4):41-42.(in Chinese)黄隆军.2004.提高菠萝催花抽薹率研究.中国南方果树,33(4):41-42.
Li Jun-ming,Zhu Deng-yun.2005.Plant tissue culture tutorial.Beijing:China Agricultural University Press:61-77.(in Chinese)李浚明,朱登云.2005.植物组织培养教程.北京:中国农业大学出版社:61-77.
Min X J,Bartholomew D P.1997.Temperature affects ethylene metabolism and fruit initiation and size of pineapple.Acta Horticulturae,425(425):329-338.
Smith L B,Downs R J.1979.Bromelioideae(Bromeliaceae).Flora Neotropica,14(3):2142.
Yao Yan-li,Zhu Zhu-ying,Yang Yu-mei,Sun Guang-ming,Zhang Xiu-mei.2016.Effect of induction flowering at different forcing date on fruit quality formation of comte de Paris pineapple.Fruit Trees in Southern China,45(2):101-105.(in Chinese)姚艳丽,朱祝英,杨玉梅,孙光明,张秀梅.2016.不同时期催花对巴厘菠萝果实品质形成的影响.中国南方果树,45(2):101-105.
Zhang Jiang-zhou,Yan Cheng-ming,Liu Ya-nan,Ma Hai-yang,Shi Wei-qi.2013.Effects of different forcing date on growth of pineapple fruit.Journal of Food and Nutrition Science,(2):25-28.(in Chinese)张江周,严程明,刘亚男,马海洋,石伟琦.2013.不同催花时期对菠萝果实生长发育的影响.食品与营养科学,(2):25-28.
Zhang Zhi-li,Fan Hong-yan,Hua Min,He Fan.2012.Induction of pineapple flowering and underlying physiological and molecular bases.Chinese Journal of Tropical Crops,33(5):950-955.(in Chinese)张治礼,范鸿雁,华敏,何凡.2012.菠萝开花诱导及其生理与分子基础.热带作物学报,33(5):950-955.
Zhu Zhu-ying,Yang Yu-mei,Sun Guang-ming,Liu Sheng-hui.2012.Natural flowering of pineapple and its suppression technology.China Tropical Agriculture,(5):75-77.(in Chinese)朱祝英,杨玉梅,孙光明,刘胜辉.2012.菠萝自然开花及其抑制技术.中国热带农业,(5):75-77.
D’Eeckenbrugge C.1993.Utilization of pineapple genetic resources in breeding reports.Tropical Fruits Newsletter,IICA-PRRET A2/TT:94-101.
Fan Shuang-xi,Li Guang-chen.2007.Horticultural plant cultivation.Beijign:China Agricultural University Press:21-32.(in Chinese)范双喜,李光晨.2007.园艺植物栽培学.北京:中国农业大学出版社:21-32.
Gong Xue.2016.Investigation on the main characters of pineapple in‘Shenwan’and selection of superior plants[M.D.Dissertation].Guangzhou:South China Agricultural University.(in Chinese)龚雪.2016.神湾菠萝主要性状调查与优株选择[硕士论文].广州:华南农业大学.
Haque S M,Ghosh B.2017.Regeneration of cytologically stable plants through dedifferentiation,redifferentiation,and artificial seeds in Spathoglottis plicata Blume.(Orchidaceae).Horticultural Plant Journal,3(5):199-208.
He Ye-hua,Fang Shao-qiu,Ma Jun,Hu Zhong-yi,Lu Min,Peng Bing.2010.Histocytology observation on the somatic embryogenesis in Ananas comosus callus.Acta Horticulturae Sinica,37(5):689-696.(in Chinese)何业华,方少秋,马均,胡中沂,卢敏,彭兵.2010.菠萝愈伤组织中体细胞胚起源过程的组织细胞学观察.园艺学报,37(5):689-696.
He Ye-hua,Hu Zhong-yi,Ma Jun,Fang Shao-qiu,Lu Min,Xie Zhi-liang,Peng Bing.2009.Germplasm resources and taxonomy of bromeliad.Nonwood Forest Research,27(3):102-107.(in Chinese)何业华,胡中沂,马均,方少秋,卢敏,谢志亮,彭兵.2009.凤梨类植物的种质资源与分类.经济林研究,27(3):102-107.
He Ye-hua,Luo Ji,Wu Hui-tao,Wang Rui-xia,Gao Ai-ping,Zhao Chun-xiang,Yu Xiao-ling,Ye Zi-xing,Wang Ze-huai,Han Jing-zhong,Liu He-ping.2007.Somatic embryogenesis from leaf base callus of Ananas comosus.Journal of Fruit Science,24(1):59-63.(in Chinese)何业华,罗吉,吴会桃,王瑞霞,高爱平,赵春香,余小玲,叶自行,王泽槐,韩景忠,刘和平.2007.菠萝叶基愈伤组织诱导体细胞胚.果树学报,24(1):59-63.
He Ye-hua,Zhang Ya-fen,Xia Jing-xian,Guo Cui-hong,Cao Li,Zhang Jun-li.2014.Screening of pineapple cold resistant cell lines.Acta Horticulturae Sinica,41(Suppl):2654.(in Chinese)何业华,张雅芬,夏靖娴,郭翠红,曹莉,张俊丽.2014.菠萝抗寒细胞株系的筛选.园艺学报,41(Suppl):2654.
Huang Long-jun.2004.Study on improving bolting rate of pineapple.Fruit Trees in Southern China,33(4):41-42.(in Chinese)黄隆军.2004.提高菠萝催花抽薹率研究.中国南方果树,33(4):41-42.
Li Jun-ming,Zhu Deng-yun.2005.Plant tissue culture tutorial.Beijing:China Agricultural University Press:61-77.(in Chinese)李浚明,朱登云.2005.植物组织培养教程.北京:中国农业大学出版社:61-77.
Min X J,Bartholomew D P.1997.Temperature affects ethylene metabolism and fruit initiation and size of pineapple.Acta Horticulturae,425(425):329-338.
Smith L B,Downs R J.1979.Bromelioideae(Bromeliaceae).Flora Neotropica,14(3):2142.
Yao Yan-li,Zhu Zhu-ying,Yang Yu-mei,Sun Guang-ming,Zhang Xiu-mei.2016.Effect of induction flowering at different forcing date on fruit quality formation of comte de Paris pineapple.Fruit Trees in Southern China,45(2):101-105.(in Chinese)姚艳丽,朱祝英,杨玉梅,孙光明,张秀梅.2016.不同时期催花对巴厘菠萝果实品质形成的影响.中国南方果树,45(2):101-105.
Zhang Jiang-zhou,Yan Cheng-ming,Liu Ya-nan,Ma Hai-yang,Shi Wei-qi.2013.Effects of different forcing date on growth of pineapple fruit.Journal of Food and Nutrition Science,(2):25-28.(in Chinese)张江周,严程明,刘亚男,马海洋,石伟琦.2013.不同催花时期对菠萝果实生长发育的影响.食品与营养科学,(2):25-28.
Zhang Zhi-li,Fan Hong-yan,Hua Min,He Fan.2012.Induction of pineapple flowering and underlying physiological and molecular bases.Chinese Journal of Tropical Crops,33(5):950-955.(in Chinese)张治礼,范鸿雁,华敏,何凡.2012.菠萝开花诱导及其生理与分子基础.热带作物学报,33(5):950-955.
Zhu Zhu-ying,Yang Yu-mei,Sun Guang-ming,Liu Sheng-hui.2012.Natural flowering of pineapple and its suppression technology.China Tropical Agriculture,(5):75-77.(in Chinese)朱祝英,杨玉梅,孙光明,刘胜辉.2012.菠萝自然开花及其抑制技术.中国热带农业,(5):75-77.