响应面法优化肉苁蓉松果菊苷肠溶微球的制备
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摘要
目的制备肉苁蓉松果菊苷肠溶微球,筛选最佳制备工艺,并考察其体外释放特性。方法用离子凝胶-干燥法制备肉苁蓉松果菊苷肠溶微球,以包封率为考察指标,用响应面法优化其制备工艺。结果制备肉苁蓉松果菊苷肠溶微球最佳工艺参数:海藻酸钠浓度36.33mg/ml、氯化钙浓度10.82mg/ml、壳聚糖浓度10.93mg/ml。结论优选的工艺稳定、可行,制备的微球包封率高、缓释效果较好。
Objective To prepare Herba Cistanche enteric release microspheres,optimize the preparation process and study the releasing characteristics of microspheres in vitro.Methods Ion gelatin-oven drying method was used to prepare Herba Cistanche enteric microspheres.The preparation process was optimized with the aid of a Box-Behnken design.Results The optimal preparation condition was 36.33 mg/ml of sodium alginate,10.82 mg/ml of calcium chloride and 10.93 mg/ml of chitosan.Conclusion This technology is repeatable and feasible.The microspheres have high entrapment efficiency and good sustained release characteristics.
Objective To prepare Herba Cistanche enteric release microspheres,optimize the preparation process and study the releasing characteristics of microspheres in vitro.Methods Ion gelatin-oven drying method was used to prepare Herba Cistanche enteric microspheres.The preparation process was optimized with the aid of a Box-Behnken design.Results The optimal preparation condition was 36.33 mg/ml of sodium alginate,10.82 mg/ml of calcium chloride and 10.93 mg/ml of chitosan.Conclusion This technology is repeatable and feasible.The microspheres have high entrapment efficiency and good sustained release characteristics.
引文
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[7]Li M,Li Y,Liu W,et al.The preparation of Cistanche phenylethanoid glycosides liquid proliposomes:Optimized formulation,characterization and proliposome dripping pills in vitro and in vivo evaluation[J].Eur J Pharm Sci,2016,93:224-232.
[8]李蒙,付艳秋,韩静,等.响应面法优化肉苁蓉苯乙醇苷回流提取工艺[J].中南药学,2015,13(8):819-823.
[9]成杰,杨鹏飞.紫外分光光度法测定哈萨克斯坦产肉苁蓉中苯乙醇总苷的含量[J].中国医院药学杂志,2002,22(10):633-633.
[2]Yang F,Wen D,Fang B,et al.Prevention of Cistanche salsa extract on hepatic fibrosis induced by carbon tetrachloride in rats[J].CHM,2013,5(3):199-204.
[3]Zhang D,Li H,Wang JB.Echinacoside inhibits amyloid fibrillization of HEWL and protects against Aβ-induced neurotoxicity[J].Int J Biol Maromol,2015,72:243-253.
[4]罗光明,黄远亮.肉苁蓉松果菊苷药理作用研究进展[J].亚太传统医药,2014,10(17):58-60.
[5]Li F,Yang X,Yang Y,et al.Phospholipid complex as an approach for bioavailability enhancement of echinacoside[J].Drug Dev Ind Pharm,2015,41(11):1777-1784.
[6]天津市弗兰德医药科技发展有限公司.一种肉苁蓉提取微丸及其制备方法[P].CN101444558.2009-06-03.
[7]Li M,Li Y,Liu W,et al.The preparation of Cistanche phenylethanoid glycosides liquid proliposomes:Optimized formulation,characterization and proliposome dripping pills in vitro and in vivo evaluation[J].Eur J Pharm Sci,2016,93:224-232.
[8]李蒙,付艳秋,韩静,等.响应面法优化肉苁蓉苯乙醇苷回流提取工艺[J].中南药学,2015,13(8):819-823.
[9]成杰,杨鹏飞.紫外分光光度法测定哈萨克斯坦产肉苁蓉中苯乙醇总苷的含量[J].中国医院药学杂志,2002,22(10):633-633.