地氯雷他定治疗新生大鼠低温肺出血疗效研究
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摘要
目的探讨第3代抗组胺药物——地氯雷他定对新生大鼠低温肺出血的治疗作用。方法 2012年2月将60只SD级新生大鼠按照完全随机法分为常温对照组(C组,20只)和低温建模组(40只);将40只低温建模组大鼠按照完全随机法分为低温对照组(L组)和地氯雷他定组(D组),每组20只。给药后于1、2、3、4 h时间点处死各组大鼠(每个时间点5只),采集标本后观察各组大鼠肺组织苏木精-伊红染色病理学改变,检测肺组织湿重/干重(W/D)比值、组胺含量,以及血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平。结果低温建模组大鼠肺组织病理学改变情况、肺组织W/D比值与C组比较,差异均有统计学意义(P <0. 05); D组大鼠肺组织病理学改变情况、肺组织W/D比值与L组比较,差异均有统计学意义(P <0. 05)。L、D组大鼠各时间点肺组织组胺水平及血清TNF-α、IL-6水平均明显高于C组,D组各时间点大鼠肺组织组胺水平及血清TNF-α、IL-6水平均明显低于L组,差异均有统计学意义(P <0. 05)。结论地氯雷他定对新生大鼠肺部出血性损伤具有保护作用,对新生大鼠低温肺出血具有一定的治疗作用。
Objective To study the therapeutic effects of third generation antihistamine drug desloratadine on lung hemorrhage induced by hypothermia in neonatal rat. Methods Sixty Sprague-Dawley( SD) grade neonatal rats were randomly divided into the normal temperature control group( group C,20 cases) and hypothermia model group( 40 cases); the hypothermia model group was randomly re-divided into the hypothermia control group( group L) and desloratadine group( group D),20 cases in each group. The rats in each group were killed at the timepoints of 1,2,3,4 h after medication( 5 cases at each time point). The HE staining pathogenic changes of lung tissue,lung wet/dry( W/D) weight ratio,histamine content and levels of TNF-α and IL-6 in serum were observed in each group. Results The lung tissue pathologic changes and lung tissue W/D ratio had statistical differences between the hypothermia model group and group C( P < 0. 05);the lung tissue pathologic changes and lung tissue W/D ratio had statistical differences between the group D and L( P < 0. 05). The histamine level of lung tissue and serum TNF-α and IL-6 levels at each time point in the group L and D were significantly higher than those in the group C( P < 0. 05),while the histamine level of lung tissue and serum TNF-α and IL-6 levels at each time point in the group D were significantly lower than those in the group L,and the differences were statistically significant( P < 0. 05). Conclusion Desloratadine has the protective effect on lung hemorrhage injury in neonatal rat and has a certain treatment effect on hypothermic lung hemorrhage in neonatal rat.
Objective To study the therapeutic effects of third generation antihistamine drug desloratadine on lung hemorrhage induced by hypothermia in neonatal rat. Methods Sixty Sprague-Dawley( SD) grade neonatal rats were randomly divided into the normal temperature control group( group C,20 cases) and hypothermia model group( 40 cases); the hypothermia model group was randomly re-divided into the hypothermia control group( group L) and desloratadine group( group D),20 cases in each group. The rats in each group were killed at the timepoints of 1,2,3,4 h after medication( 5 cases at each time point). The HE staining pathogenic changes of lung tissue,lung wet/dry( W/D) weight ratio,histamine content and levels of TNF-α and IL-6 in serum were observed in each group. Results The lung tissue pathologic changes and lung tissue W/D ratio had statistical differences between the hypothermia model group and group C( P < 0. 05);the lung tissue pathologic changes and lung tissue W/D ratio had statistical differences between the group D and L( P < 0. 05). The histamine level of lung tissue and serum TNF-α and IL-6 levels at each time point in the group L and D were significantly higher than those in the group C( P < 0. 05),while the histamine level of lung tissue and serum TNF-α and IL-6 levels at each time point in the group D were significantly lower than those in the group L,and the differences were statistically significant( P < 0. 05). Conclusion Desloratadine has the protective effect on lung hemorrhage injury in neonatal rat and has a certain treatment effect on hypothermic lung hemorrhage in neonatal rat.
引文
[1]陈克正,齐文玉.新生儿肺出血的临床诊断与病理类型[J].中华儿科杂志,1996,34(1):63-64.
[2]刘一平.新生儿硬肿症并发肺出血的发病机理及预防探讨[J].医学信息,1995,8(1):32-33.
[3]刘春峰,郭宏湘,袁壮,等.低体温新生大鼠肺内组胺变化与肺损伤关系的探讨[J].中华儿科杂志,2001,39(1):53-54.
[4]吴春英,游上游,鲁建生,等.肺出血新生大鼠肺组织组胺水平的变化[J].临床儿科杂志,2009,27(7):670-672.
[5]张林丽,何建林,余书勤.卢帕他定对大鼠油酸型急性肺损伤的保护作用研究[J].中国药学杂志,2008,43(6):420-423.
[6]欧德渊,付学智,高铭宇,等.两种组胺受体拮抗剂对内毒素诱导雏鸡肺损伤的影响[J].畜牧兽医学报,2009,40(4):566-571.
[7]陈克正,王卫.新生大鼠肺出血动物模型的建立及对临床的启示[J].中国当代儿科杂志,2002,4(5):357-360.
[8]付萌,樊平申,赵小东. 3种抗组胺药抗花生四烯酸致炎性的比较[J].临床皮肤科杂志,2004,33(11):662-664.
[9]王丽,谭焰,谷伟,等.慢性阻塞性肺病缓解期患者血清中TNF-α、IL-6和IL-8表达的研究[J].临床肺科杂志,2011,16(6):835-836.
[10] MURAKAMI K,OKAJIMA K,UCHIBA M. The prevention of lipopolysaceharide induced pulmonary vascular injury by pretreament with cepharathine on rat[J]. Am J Respir Crit Care Med,2000,161(1):57-63.
[11]金勤立,徐锦,孙家娥,等.应用不同配方磷脂预防大鼠肺出血和肺水肿的研究[J].中华儿科杂志,1997,35(2):71-73.
[12]戴顺龄,苏树芸,程凯,等.川芎嗪防治大鼠实验性肺水肿的电镜观察[J].中西医结合杂志,1987,7(11):676-678.
[13]李娟,李书琴,吴红敏.肺出血幼兔右心室压力及肺毛细血管内皮细胞微结构的改变[J].中华儿科杂志,2002,38(3):147-149.
[14]陈克正.新生儿肺出血几个问题的探讨[J].中华儿科杂志,2004,24(5):136-138.
[15]陈克正.新生大鼠肺出血动物模型的建立[J].中国当代儿科杂志,2001,21(5):35-36.
[16]赵克森,黄巧冰.血管通透性增高的基本机制[J].中国病理生理杂志,2003,19(4):549-553.
[17] BAYRAM H,DEVALIA JL,KHAIR OA,et al. Effect of loratadine on nitrogen dioxide-induced changes in electrical resistance and release of inflammatory mediators from culture human bronchial epitthelial cells[J]. J Allergy Clin Immunol,1999,104(1):93-99.
[18] XIE GQ,JIANG JX,CHEN YH,et al. Induction of acute hepatic injure by endotoxin in mice[J]. Hepatobiliary Pancreat Dis Int,2002,1(4):558-564.
[19] AOSASA S,ONO S,MOCHIZUKI H,et al. Mechanism of the inhibitory effect of protease inhibitor on tumor necrosis factor alpha production of monocytes[J]. Shock,2001,15(2):101-105.
[20] WALLEY KR,MCDONALD TE,HIGASHIMOTO Y,et al.Modulation of proinflammatory cytokines by nitric oxide in murine acute lung injury[J]. Am J Respir Crit Care Med,1999,160(2):698-704.
[21] CHART EL,HAUDEK SB,GIROIR BP,et al. Human coronary endothelial cellactivation by endotoxin is characterized by NF-kB activation and TNF-a synthesis[J]. Shock,2001,16:349-354.
[22] ST JOHN RC,DORINSKY PM. Immunologic therapy for ARDS septic shock and multipleorgan failure[J]. Chest,1993,103(3):932-943.
[23] CHEN LY,ZURAW BL,ZHAO M,et al. Involment of protein tyrosine kinase in Toll-like receptor 4-mediated NF-kappa B activation inhuman peripheral bloodmonocytes[J]. Am J Physiol Lung Cell Mol Physiol,2003,284(4):607-613.
[24]徐文严.地氯雷他定:一种新的抗组胺药[J].中华皮肤科杂志,2003,36(11):668-670.
[25] LIPPERT U,MLLER A,WELKER P,et al. Inhibition of cytokine secretion from human leukemic mast cells and basophilis by H1-and H2-receptor antagonists[J]. Exp Dermatal,2009,9(2):118-124.
[2]刘一平.新生儿硬肿症并发肺出血的发病机理及预防探讨[J].医学信息,1995,8(1):32-33.
[3]刘春峰,郭宏湘,袁壮,等.低体温新生大鼠肺内组胺变化与肺损伤关系的探讨[J].中华儿科杂志,2001,39(1):53-54.
[4]吴春英,游上游,鲁建生,等.肺出血新生大鼠肺组织组胺水平的变化[J].临床儿科杂志,2009,27(7):670-672.
[5]张林丽,何建林,余书勤.卢帕他定对大鼠油酸型急性肺损伤的保护作用研究[J].中国药学杂志,2008,43(6):420-423.
[6]欧德渊,付学智,高铭宇,等.两种组胺受体拮抗剂对内毒素诱导雏鸡肺损伤的影响[J].畜牧兽医学报,2009,40(4):566-571.
[7]陈克正,王卫.新生大鼠肺出血动物模型的建立及对临床的启示[J].中国当代儿科杂志,2002,4(5):357-360.
[8]付萌,樊平申,赵小东. 3种抗组胺药抗花生四烯酸致炎性的比较[J].临床皮肤科杂志,2004,33(11):662-664.
[9]王丽,谭焰,谷伟,等.慢性阻塞性肺病缓解期患者血清中TNF-α、IL-6和IL-8表达的研究[J].临床肺科杂志,2011,16(6):835-836.
[10] MURAKAMI K,OKAJIMA K,UCHIBA M. The prevention of lipopolysaceharide induced pulmonary vascular injury by pretreament with cepharathine on rat[J]. Am J Respir Crit Care Med,2000,161(1):57-63.
[11]金勤立,徐锦,孙家娥,等.应用不同配方磷脂预防大鼠肺出血和肺水肿的研究[J].中华儿科杂志,1997,35(2):71-73.
[12]戴顺龄,苏树芸,程凯,等.川芎嗪防治大鼠实验性肺水肿的电镜观察[J].中西医结合杂志,1987,7(11):676-678.
[13]李娟,李书琴,吴红敏.肺出血幼兔右心室压力及肺毛细血管内皮细胞微结构的改变[J].中华儿科杂志,2002,38(3):147-149.
[14]陈克正.新生儿肺出血几个问题的探讨[J].中华儿科杂志,2004,24(5):136-138.
[15]陈克正.新生大鼠肺出血动物模型的建立[J].中国当代儿科杂志,2001,21(5):35-36.
[16]赵克森,黄巧冰.血管通透性增高的基本机制[J].中国病理生理杂志,2003,19(4):549-553.
[17] BAYRAM H,DEVALIA JL,KHAIR OA,et al. Effect of loratadine on nitrogen dioxide-induced changes in electrical resistance and release of inflammatory mediators from culture human bronchial epitthelial cells[J]. J Allergy Clin Immunol,1999,104(1):93-99.
[18] XIE GQ,JIANG JX,CHEN YH,et al. Induction of acute hepatic injure by endotoxin in mice[J]. Hepatobiliary Pancreat Dis Int,2002,1(4):558-564.
[19] AOSASA S,ONO S,MOCHIZUKI H,et al. Mechanism of the inhibitory effect of protease inhibitor on tumor necrosis factor alpha production of monocytes[J]. Shock,2001,15(2):101-105.
[20] WALLEY KR,MCDONALD TE,HIGASHIMOTO Y,et al.Modulation of proinflammatory cytokines by nitric oxide in murine acute lung injury[J]. Am J Respir Crit Care Med,1999,160(2):698-704.
[21] CHART EL,HAUDEK SB,GIROIR BP,et al. Human coronary endothelial cellactivation by endotoxin is characterized by NF-kB activation and TNF-a synthesis[J]. Shock,2001,16:349-354.
[22] ST JOHN RC,DORINSKY PM. Immunologic therapy for ARDS septic shock and multipleorgan failure[J]. Chest,1993,103(3):932-943.
[23] CHEN LY,ZURAW BL,ZHAO M,et al. Involment of protein tyrosine kinase in Toll-like receptor 4-mediated NF-kappa B activation inhuman peripheral bloodmonocytes[J]. Am J Physiol Lung Cell Mol Physiol,2003,284(4):607-613.
[24]徐文严.地氯雷他定:一种新的抗组胺药[J].中华皮肤科杂志,2003,36(11):668-670.
[25] LIPPERT U,MLLER A,WELKER P,et al. Inhibition of cytokine secretion from human leukemic mast cells and basophilis by H1-and H2-receptor antagonists[J]. Exp Dermatal,2009,9(2):118-124.
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