基于低功耗蓝牙传输的电位型嵌入式无线传感监测系统的研制
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摘要
设计并制作了一种可用于电位型传感器的小型嵌入式无线监测系统,此系统以精密双路运放TLC4502为前置放大器,低功耗蓝牙(BLE)芯片CC2541作为主控制器,主控制器集成14位模数转换器(ADC)。此系统使用BLE开发应用程序(App),与CC2541建立蓝牙连接,实现对传感电位的无线实时读取。监测系统软件部分由嵌入式开发软件IAR Embedded Workbench在BLE-CC254X-1.41协议栈基础上开发。为验证系统可靠性与精度,采用精密稳压电源进行模拟电位采集测试,并使用p H复合电极对pH值变化进行监测。测试结果表明,此系统能对电位进行实时测量,信号放大3倍,精度达到0.4 mV,对pH值变化能快速输出一致性响应,监测响应的线性相关系数R~2=0.9994;同时,应用自制的L-半胱氨酸(L-cys)传感器结合本系统对L-cys浓度变化进行实时检测,动态响应变化与商品化数字万用表的结果一致,进一步表明此蓝牙监测系统可实现电位型传感信号的快速传输与实时监测。
A small embedded wireless monitoring system for potentiometric sensors was designed and fabricated. The system employed a dual precision op amp of TLC4502 as the preamplifier and a bluetooth low energy (BLE) chip of CC2541 as the main controller,and the main controller integrated 14 bits analog to digital convertor (ADC). The system used the BLE development application program (App) to establish a Bluetooth connection with the CC2541 for wireless real-time reading of sensing potentials. The monitoring system software was developed on the basis of a BLE-CC254X-1. 41 protocol stack by the embedded development software of IAR Embedded Workbench. To verify the reliability and accuracy of the system,a precision regulated power supply was used for analog potential acquisition test,and a p H composite electrode was used for monitoring the pH change. The test results showed that the system could be applied to real time measurement of potential signals for three times amplification with an accuracy of 0. 4 mV,and a consistent response to p H change was able to quickly output with a linear correlation coefficient of R~2= 0. 9994.Furthermore,the concentration change of L-cysteine (L-cys) was monitored in real time by the BLE system with a self-made L-cys sensor,and the dynamic response change was consistent with that of commercial multimeter,indicating that the rapid transmission and real time monitoring of potentiometric signals could be well realized through the BLE wireless sensing system.
A small embedded wireless monitoring system for potentiometric sensors was designed and fabricated. The system employed a dual precision op amp of TLC4502 as the preamplifier and a bluetooth low energy (BLE) chip of CC2541 as the main controller,and the main controller integrated 14 bits analog to digital convertor (ADC). The system used the BLE development application program (App) to establish a Bluetooth connection with the CC2541 for wireless real-time reading of sensing potentials. The monitoring system software was developed on the basis of a BLE-CC254X-1. 41 protocol stack by the embedded development software of IAR Embedded Workbench. To verify the reliability and accuracy of the system,a precision regulated power supply was used for analog potential acquisition test,and a p H composite electrode was used for monitoring the pH change. The test results showed that the system could be applied to real time measurement of potential signals for three times amplification with an accuracy of 0. 4 mV,and a consistent response to p H change was able to quickly output with a linear correlation coefficient of R~2= 0. 9994.Furthermore,the concentration change of L-cysteine (L-cys) was monitored in real time by the BLE system with a self-made L-cys sensor,and the dynamic response change was consistent with that of commercial multimeter,indicating that the rapid transmission and real time monitoring of potentiometric signals could be well realized through the BLE wireless sensing system.
引文
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13 Cao Z,Zheng Y L,Gong F C,Long S,Chen P,He X C.Microchem.J.,2007,86(1):71-79
14 Bakker E,Qin Y.Anal.Chem,2006,78(12):3965-3984
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16 Toczyowska-Mamińska R,Kloch M,Zawistowska-Deniziak A,Bala A.Talanta,2016,159:7-13
17 Cai Q Y,Zeng K F,Ruan C,Desai T A,Grimes C A.Anal.Chem.,2004,76(14):4038-4043
18 Sheng Y Y,You Y,Cao Z,Liu L,Wu H C.Analyst,2018,143:2411-2415
19 Shantaram A,Beyenal H,Veluchamy R R A,Lewandowski Z.Environ.Sci.Technol.,2005,39(13):5037-5042
20 Curto V F,Fay C,Coyle S,Byrne R,O'Toole C,Barry C,Hughes S,Moyna N,Diamond D,Benito-Lopez F.Sens.Actuators B,2012,171-172(8):1327-1334
21 Kasasbeh A,Lee K,Bieber A,Benneta K,Chang S Y.Stereotact.Funct.Neurosurg.,2013,91(3):141-147
22 XIAO Qing,CHEN Lin,LI Wen-Feng,YANG Li-Qin,CAO Zhong,YU Xin-Yao,LONG Shu,HE Jing-Lin,XIAOZhong-Liang.Chinese J.Anal.Chem.,2018,46(12):1886-1894肖情,陈琳,李文锋,杨丽琴,曹忠,于鑫垚,龙姝,何婧琳,肖忠良.分析化学,2018,46(12):1886-1894
23 LIN Nan-Sen,WANG Li,WANG Mi-Xia,XU Sheng-Wei,YU Wei-Dong,CAI Xin-Xia.Chinese J.Anal.Chem.,2015,43(1):93-97林楠森,王力,王蜜霞,徐声伟,禹卫东,蔡新霞.分析化学,2015,43(1):93-97
24 FAN Yan,WANG Yang,LIU Jun-Tao,LUO Jin-Ping,XU Hui-Ren,XU Sheng-Wei,CAI Xin-Xia.Chinese J.Anal.Chem.,2016,44(7):1148-1154樊艳,王杨,刘军涛,罗金平,徐辉任,徐声伟,蔡新霞.分析化学,2016,44(7):1148-1154
25 SONG Xian-Teng,XU Sheng-Wei,WANG Mi-Xia,ZHANG Song,WANG Li,CAI Xin-Xia.Transducer and Microsystem Technologies,2017,36(4):117-120宋先腾,徐声伟,王蜜霞,张松,王力,蔡新霞.传感器与微系统,2017,36(4):117-120
26 ZENG Li-Zhi.Software,2013,34(7):63-64曾立志.软件,2013,34(7):63-64
27 ZHENG Gui-Lin,TAO Zhi-Hao.Chinese Journal of Sensors and Actuators,2017,30(2):167-173郑贵林,陶志浩.传感技术学报,2017,30(2):167-173
28 Filho G P R,Villas L A,Freitas H,Valejo A,Guidoni D L,Ueyama J.Computer Networks,2018,135:54-69
29 de Blasio G,Quesada-Arencibia A,García C R,Molina-Gil J M,Caballero-Gil C.Sensors,2017,17(6):1299-1327
30 QIN Tai-Chun,LI Xiao Gang,HAO Jie,YU Ping,MAO Lan-Qun.Chinese J.Anal.Chem.,2015,43(3):457-462秦泰春,李晓钢,郝洁,于萍,毛兰群.分析化学,2015,43(3):457-462
31 LI Xiao-Gang,GUO Bin-Qian,QIN Tai-Chun,HAO Jie,YU Ping,MAO Lan-Qun.Chinese J.Anal.Chem.,2016,44(9):1465-1470李晓钢,郭彬乾,秦泰春,郝洁,于萍,毛兰群.分析化学,2016,44(9):1465-1470
32 Nemiroski A,Christodouleas D C,Hennek J W,Kumar A A,Maxwell E J,Fernández-Abedul M T,Whitesides G M.Proc.Natl.Acad.Sci.USA,2014,111(33):11984-11989
33 Kaisti M,Boeva Z,Koskinen J,Nieminen S,Bobacka J,Levon K.ACS Sensors,2016,1(12):1423-1431
34 Gu Z,Liu H X,Ying Y L,Xiu G L,Long Y T.Analyst,2018,143(12):2760-2764
35 Ainla A,Mousavi M P S,Tsaloglou M N,Redston J,Bell J G,Fernández-Abedul M T,Whitesides G M.Anal.Chem.,2018,90(10):6240-6246
36 Jung J,Lee J,Shin S,Kim Y T.Sensors,2017,17(10):2416-2424
37 Fan Y,Liu J T,Wang Y,Luo J P,Xu H R,Xu S W,Cai X X.Biosens.Bioelectron.,2017,95:60-66
38 TLC4502 Data Manual,Texas Instruments Inc,2001.http://www.ti.com/product/cn/TLC4502A
39 CC2541 Data Manual,Texas Instruments Inc,2013.http://www.ti.com.cn/product/cn/cc2541
40 OU Yang-Jun,CHEN Zi-Long,HUANG Ning-Lin.Bluetooth 4.0 BLE Development Complete Manual:Internet of Things Development Technology.Beijing:Chemical Industry Press,2013欧阳骏,陈子龙,黄宁淋.蓝牙4.0 BLE开发完全手册:物联网开发技术实战.北京:化学工业出版社,2013
2 XIE Sai-Dan,LIU Yang,WU Zhao-Yang,SHEN Guo-Li,YU Ru-Qin.Chinese J.Anal.Chem.,2015,43(11):1648-1655谢赛丹,刘洋,吴朝阳,沈国励,俞汝勤.分析化学,2015,43(11):1648-1655
3 AN Qing-Bo,JIA Fei,XU Jia-Nan,LI Feng-Hua,NIU Li.Scientia Sinica Chimica,2017,47(5):524-531安清波,贾菲,许佳楠,李风华,牛利.中国科学:化学,2017,47(5):524-531
4 Crespo G A.Electrochim.Acta,2017,245:1023-1034
5 YU Ru-Qin.Ion Selective Electrode Analysis.Beijing:People's Education Publishing House,1980俞汝勤.离子选择性电极分析法.北京:人民教育出版社,1980
6 Yu X,Zhou Z,Wang Y,Liu Y,Xie Q,Xiao D.Sens.Actuators B,2007,123(1):352-358
7 CAO Ting-Ting,CAO Zhong,HE Jing-Lin,LIANG Hai-Qin,XIAO Zhong-Liang.Chem.J.Chinese Univ.,2014,35(7):1388-1395曹婷婷,曹忠,何婧琳,梁海琴,肖忠良.高等学校化学学报,2014,35(7):1388-1395
8 CUI Guang-Wen,HE Run-He,YAO Jian,WANG Jian-Ping,ZHANG Xing-Xiang.Chinese J.Anal.Chem.,2018,46(10):1669-1676崔光文,何润合,药健,王建平,张兴祥.分析化学,2018,46(10):1669-1676
9 Ma Y H,Yuan R,Chai Y Q,Wu X,Zhou W,Liu X L,Deng F.Anal.Lett.,2009,42(15):2411-2429
10 Li X Q,Liang H Q,Cao Z,Xiao Q,Xiao Z L,Song L B,Chen D,Wang F L.Mater.Sci.Eng.C,2017,72:26-33
11 Bakker E.Anal.Chem,2016,88(1):395-413
12 Cao Z,Gong F C,Li H P,Xiao Z L,Long S,Zhang L,Peng S J.Anal.Chim.Acta,2007,581(1):19-26
13 Cao Z,Zheng Y L,Gong F C,Long S,Chen P,He X C.Microchem.J.,2007,86(1):71-79
14 Bakker E,Qin Y.Anal.Chem,2006,78(12):3965-3984
15 Cao Z,Lin H G,Wang B F,Chen Z Z,Ma F L,Wang K M,Yu R Q.Anal.Lett.,1995,28(3):451-466
16 Toczyowska-Mamińska R,Kloch M,Zawistowska-Deniziak A,Bala A.Talanta,2016,159:7-13
17 Cai Q Y,Zeng K F,Ruan C,Desai T A,Grimes C A.Anal.Chem.,2004,76(14):4038-4043
18 Sheng Y Y,You Y,Cao Z,Liu L,Wu H C.Analyst,2018,143:2411-2415
19 Shantaram A,Beyenal H,Veluchamy R R A,Lewandowski Z.Environ.Sci.Technol.,2005,39(13):5037-5042
20 Curto V F,Fay C,Coyle S,Byrne R,O'Toole C,Barry C,Hughes S,Moyna N,Diamond D,Benito-Lopez F.Sens.Actuators B,2012,171-172(8):1327-1334
21 Kasasbeh A,Lee K,Bieber A,Benneta K,Chang S Y.Stereotact.Funct.Neurosurg.,2013,91(3):141-147
22 XIAO Qing,CHEN Lin,LI Wen-Feng,YANG Li-Qin,CAO Zhong,YU Xin-Yao,LONG Shu,HE Jing-Lin,XIAOZhong-Liang.Chinese J.Anal.Chem.,2018,46(12):1886-1894肖情,陈琳,李文锋,杨丽琴,曹忠,于鑫垚,龙姝,何婧琳,肖忠良.分析化学,2018,46(12):1886-1894
23 LIN Nan-Sen,WANG Li,WANG Mi-Xia,XU Sheng-Wei,YU Wei-Dong,CAI Xin-Xia.Chinese J.Anal.Chem.,2015,43(1):93-97林楠森,王力,王蜜霞,徐声伟,禹卫东,蔡新霞.分析化学,2015,43(1):93-97
24 FAN Yan,WANG Yang,LIU Jun-Tao,LUO Jin-Ping,XU Hui-Ren,XU Sheng-Wei,CAI Xin-Xia.Chinese J.Anal.Chem.,2016,44(7):1148-1154樊艳,王杨,刘军涛,罗金平,徐辉任,徐声伟,蔡新霞.分析化学,2016,44(7):1148-1154
25 SONG Xian-Teng,XU Sheng-Wei,WANG Mi-Xia,ZHANG Song,WANG Li,CAI Xin-Xia.Transducer and Microsystem Technologies,2017,36(4):117-120宋先腾,徐声伟,王蜜霞,张松,王力,蔡新霞.传感器与微系统,2017,36(4):117-120
26 ZENG Li-Zhi.Software,2013,34(7):63-64曾立志.软件,2013,34(7):63-64
27 ZHENG Gui-Lin,TAO Zhi-Hao.Chinese Journal of Sensors and Actuators,2017,30(2):167-173郑贵林,陶志浩.传感技术学报,2017,30(2):167-173
28 Filho G P R,Villas L A,Freitas H,Valejo A,Guidoni D L,Ueyama J.Computer Networks,2018,135:54-69
29 de Blasio G,Quesada-Arencibia A,García C R,Molina-Gil J M,Caballero-Gil C.Sensors,2017,17(6):1299-1327
30 QIN Tai-Chun,LI Xiao Gang,HAO Jie,YU Ping,MAO Lan-Qun.Chinese J.Anal.Chem.,2015,43(3):457-462秦泰春,李晓钢,郝洁,于萍,毛兰群.分析化学,2015,43(3):457-462
31 LI Xiao-Gang,GUO Bin-Qian,QIN Tai-Chun,HAO Jie,YU Ping,MAO Lan-Qun.Chinese J.Anal.Chem.,2016,44(9):1465-1470李晓钢,郭彬乾,秦泰春,郝洁,于萍,毛兰群.分析化学,2016,44(9):1465-1470
32 Nemiroski A,Christodouleas D C,Hennek J W,Kumar A A,Maxwell E J,Fernández-Abedul M T,Whitesides G M.Proc.Natl.Acad.Sci.USA,2014,111(33):11984-11989
33 Kaisti M,Boeva Z,Koskinen J,Nieminen S,Bobacka J,Levon K.ACS Sensors,2016,1(12):1423-1431
34 Gu Z,Liu H X,Ying Y L,Xiu G L,Long Y T.Analyst,2018,143(12):2760-2764
35 Ainla A,Mousavi M P S,Tsaloglou M N,Redston J,Bell J G,Fernández-Abedul M T,Whitesides G M.Anal.Chem.,2018,90(10):6240-6246
36 Jung J,Lee J,Shin S,Kim Y T.Sensors,2017,17(10):2416-2424
37 Fan Y,Liu J T,Wang Y,Luo J P,Xu H R,Xu S W,Cai X X.Biosens.Bioelectron.,2017,95:60-66
38 TLC4502 Data Manual,Texas Instruments Inc,2001.http://www.ti.com/product/cn/TLC4502A
39 CC2541 Data Manual,Texas Instruments Inc,2013.http://www.ti.com.cn/product/cn/cc2541
40 OU Yang-Jun,CHEN Zi-Long,HUANG Ning-Lin.Bluetooth 4.0 BLE Development Complete Manual:Internet of Things Development Technology.Beijing:Chemical Industry Press,2013欧阳骏,陈子龙,黄宁淋.蓝牙4.0 BLE开发完全手册:物联网开发技术实战.北京:化学工业出版社,2013