美洲大蠊多肽对MFC荷瘤小鼠外周血淋巴细胞的影响
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摘要
目的:研究美洲大蠊多肽对小鼠胃癌细胞(MFC)荷瘤小鼠外周血淋巴细胞的影响,探索其抗肿瘤的机制。方法:体外培养MFC,建立MFC荷瘤BALB/c小鼠模型,随机分成模型组、CTX组、美洲大蠊精制物CⅡ-3高剂量与低剂量组、短肽HFDT1组,另设正常对照组,给予为期10 d的药物处理。给药结束后运用全自动血细胞计数仪及流式细胞术分析外周血淋巴细胞的变化。结果:CⅡ-3和短肽HFDT1升高了荷瘤小鼠外周血总淋巴细胞、B淋巴细胞和T淋巴细胞及其亚群的含量,不降低外周血NK细胞含量。结论:美洲大蠊精制物CⅡ-3和短肽HFDT1增强了荷瘤小鼠特异性免疫和非特异免疫的抗肿瘤作用。
Objective: In order to study the influence of Periplanteta americana peptide on lymphocytes in the peripheral blood of MFC tumor bearing mice and explore its anti-tumor mechanism. Methods: Mouse gastric cancer cells(MFC)were cultured in vitro.We established a subcutaneous tumor model in BALB/c mice with MFC cells,and divided them into 5 groups including model group,CTX group,the Periplanteta americana peptide extract CⅡ-3 high and low dose groups,synthetic peptide HFDT1 group, and normal BALB/c mice were used as normal control group. The model group and the normal group were given normal saline by gavage, while other groups were administrated with corresponding drugs for 10 days. The changes of lymphocytes in the peripheral blood were analyzed by automatic blood cell counter and flow cytometry after administration. Results: CⅡ-3 and short peptide HFDT1 increased the number of lymphocytes,and the ratios of B cell,T cell and its subsets, meanwhile they did not decrease the content of NK cell in the peripheral blood. Conclusion: Periplaneta americana extract C effects of specific immunity and non-specific immunity in tumor bearing mice.
Objective: In order to study the influence of Periplanteta americana peptide on lymphocytes in the peripheral blood of MFC tumor bearing mice and explore its anti-tumor mechanism. Methods: Mouse gastric cancer cells(MFC)were cultured in vitro.We established a subcutaneous tumor model in BALB/c mice with MFC cells,and divided them into 5 groups including model group,CTX group,the Periplanteta americana peptide extract CⅡ-3 high and low dose groups,synthetic peptide HFDT1 group, and normal BALB/c mice were used as normal control group. The model group and the normal group were given normal saline by gavage, while other groups were administrated with corresponding drugs for 10 days. The changes of lymphocytes in the peripheral blood were analyzed by automatic blood cell counter and flow cytometry after administration. Results: CⅡ-3 and short peptide HFDT1 increased the number of lymphocytes,and the ratios of B cell,T cell and its subsets, meanwhile they did not decrease the content of NK cell in the peripheral blood. Conclusion: Periplaneta americana extract C effects of specific immunity and non-specific immunity in tumor bearing mice.
引文
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[2]欧红利,常旭,王聪,等.美洲大蠊多肽对L1210荷瘤小鼠抗肿瘤免疫的初步研究[J].中国药学杂志,2018,53(5):16-21.
[3]常旭,王聪,欧红利,等.美洲大蠊多肽对MFC荷瘤小鼠免疫影响的初步探究[J].免疫学杂志,2017,33(7):564-569.
[4]赵微.美洲大蠊提取物在抗肿瘤中的免疫调节作用[D].大理:大理大学,2015.
[5]WEN R,CHEN Y,BAI L,et al.Essential Role of Phospholipase Cγ2 in Early B-Cell Development and Myc-Mediated Lymphomagenesis[J].Molecular and Cellular Biology,2006,26(24):9364-9376.
[6]KIM H W,KIM J E,HWANG M H,et al.Enhancement of natural killer cell cytotoxicity by sodium/iodide symporter gene-mediated radioiodine pretreatment in breast cancer cells[J].PLoS ONE,2013,8(8):e70194.
[7]WEI H C,YU J L,HSU C Y.Periodically Pulsed Immunotherapy in a Mathematical Model of Tumor,CD4+T Cells,and Antitumor Cytokine Interactions[J].Comput Math Methods Med,2017,2017(11):1-12.
[8]ZHANG T,SOMASUNDARAM R,BERENCSI K,et al.Migration of cytotoxic T lymphocytes toward melanoma cells in three-dimensional organotypic culture is dependent on CCL2 and CCR4[J].Eur J Immunol,2006,36(2):457-467.
[9]SHIRANI K,HASSANI F V,RAZAVI-AZARKHIAVI K,et al.Phytotrapy of cyclophosphamide-induced immunosuppression[J].Environ Toxicol Pharmacol,2015,39(3):1262-1275.
[10]刘继彦,李永强,彭瑞清,等.环磷酰胺单次注射对小鼠淋巴细胞及其亚群的早期作用[J].中国肿瘤生物治疗杂志,2005,12(3):179-182.
[11]WEI M,SHEN D,MULMI SHRESTHA S,et al.The Progress of T Cell Immunity Related to Prognosis in Gastric Cancer[J].Bio Med Research International,2018,2018(2):1-6.
[12]TOMITA Y,NISHIMURA Y.Long peptide-based cancer immunotherapy targeting tumor antigen-specific CD4+and CD8+T cells[J].Oncoimmunology,2013,2(9):e25801.
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