响应面法优化解体好氧颗粒污泥的修复方式
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摘要
通过小试实验,探究提高氨氮、COD浓度、水力剪切力以及投加活性污泥和活性炭粉末对解体好氧颗粒污泥的修复效果,得出进水COD浓度以及投加活性污泥和活性炭对颗粒修复影响较大但单一修复方式效果不理想,进而针对这3个因素,采用响应面法得出其最优耦合修复工况(进水COD、投加活性炭和活性污泥质量浓度分别340mg/L、4.64g/L和2900mg/L).经过17d运行,解体AGS得到良好修复并通过扫描电镜(SEM)对其形态进行观察,可知修复后AGS表面以丝状菌为主,孔隙、裂痕大幅减少,污泥以活性炭为晶核形成新的AGS并且颗粒修复后粒径由(0.89±0.5)mm快速增加到了(2.19±0.4)mm,氨氧化速率(以LVSS记)由2.49mg/(g·h)提高到3.18mg/(g·h),由此验证了这种耦合修复方式对解体AGS具有高效且快速的修复作用.
The research exploring the restorative effects of Ammonia nitrogen, the Chemical oxygen demand concentration, Hydraulic shear force, adding sludge and Activated carbon powder for disintegrating the aerobic granular sludge, got the results firstly that the influent COD concentration and activated sludge and activated carbon had great influence on the restorative effects, but the effect of a single repair method was not unsatisfactory; secondly, taking the way of responding surface analysis concluded that the restoration of coupling was the best way with the Influent COD of 340 mg/L, the activated carbon of 4.64 g/L and activated sludge quality concentrations 2900 mg/L. With 17 days of operation, the disintegration of AGS has gotten the positive restorative effects. Observing the morphology from the appearance and scanning electron microscopy, it was found that the filamentous bacteria have dominated the surface of AGS,. simultaneously, the pore and fissure were greatly reduced, and the sludge formed new AGS with activated carbon as crystal nucleus The particle size increased rapidly from(0.89±0.5)mm to(2.19±0.4)mm, the rate of ammonia oxidation(in LVSS) has been increased from 2.49 mg/(g·h) to 3.18 mg/(g·h), which proved that the restoration of coupling had the positive and rapid effect on disintegrating AGS.
The research exploring the restorative effects of Ammonia nitrogen, the Chemical oxygen demand concentration, Hydraulic shear force, adding sludge and Activated carbon powder for disintegrating the aerobic granular sludge, got the results firstly that the influent COD concentration and activated sludge and activated carbon had great influence on the restorative effects, but the effect of a single repair method was not unsatisfactory; secondly, taking the way of responding surface analysis concluded that the restoration of coupling was the best way with the Influent COD of 340 mg/L, the activated carbon of 4.64 g/L and activated sludge quality concentrations 2900 mg/L. With 17 days of operation, the disintegration of AGS has gotten the positive restorative effects. Observing the morphology from the appearance and scanning electron microscopy, it was found that the filamentous bacteria have dominated the surface of AGS,. simultaneously, the pore and fissure were greatly reduced, and the sludge formed new AGS with activated carbon as crystal nucleus The particle size increased rapidly from(0.89±0.5)mm to(2.19±0.4)mm, the rate of ammonia oxidation(in LVSS) has been increased from 2.49 mg/(g·h) to 3.18 mg/(g·h), which proved that the restoration of coupling had the positive and rapid effect on disintegrating AGS.
引文
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[23]张亮,张树军,彭永臻.污水处理中游离氨对硝化作用抑制影响研究[J].哈尔滨工业大学学报,2012,44(2):75-79.
[24]Yan L,Hu H,Zhang S,et al.Arsenic tolerance and bioleaching fromrealgar based on response surface methodology by Acidithiobacillus ferrooxidans isolated from Wudalianchi volcanic lake,northeast China[J].Electronic Journal of Biotechnology,2017,25:50-57.
[25]王昌稳,李军,赵白航,等.AGS的快速培养与污泥特性分析[J].中南大学学报(自然科学版),2013,44(6):2623-2628.
[26]Liu Y,Yang S F,Liu Q S,et al.The role of cell hydrophobicity in the formation of aerobic granules[J].Curr.Microbiol.,2003,46(4):270-274.
[27]Tay J H,Liu Q S,Liu Y.Characteristics of aerobic granules grown on glucose and acetate in sequential aerobic sludge blanket reactors[J].Environmental Technology,2002,23(8):931-936.
[2]汪善全,孔云华,原媛,等.AGS中丝状微生物生长研究[J].环境科学,2008,29(3):696-702.
[3]Chen Y,Jiang W,Liang D T,et al.Aerobic granulation under the combined hydraulic and loading selection pressures[J].Bioresource Technology,2008,99(16):7444-7449.
[4]Zheng Y,Yu H,Liu S,et al.Formation and instability of aerobic granules under high organic loading conditions[J].Chemosphere,2006,63(10):1791-1800.
[5]Moy B,Tay J H,Toh S K,et al.High organic loading influences the physical characteristics of aerobic sludge granules[J].Letters In Applied Microbiology,2002,34(6):407-412.
[6]龙焙,濮文虹,杨昌柱,等.失稳AGS在SBR中的修复研究[J].中国给水排水,2015,31(7):29-33.
[7]王新华,张捍民,杨凤林,等.解体AGS修复[J].大连理工大学学报,2010,50(2):183-187.
[8]彭永臻,吴蕾,马勇,等.AGS的形成机制、特性及应用研究进展[J].环境科学,2010,31(2):273-281.
[9]Adav S S,Lee D J,Lai J Y.Effects of aeration intensity on formation of phenol-fed aerobic granules and extracellular polymeric substances[J].Appl.Microbiol.Biotechnol.,2007,77(1):175-182.
[10]刘小朋,王建芳,钱飞跃,等.提高有机负荷对AGS形成与稳定过程的影响[J].环境科学,2015,(9):3352-3357.
[11]魏燕杰,季民,李国一,等.投加粉末活性炭强化AGS的稳定性[J].天津大学学报,2012,45(3):247-253.
[12]高景峰,张倩,王金惠,等.颗粒活性炭对SBR反应器中AGS培养的影响研究[J].应用基础与工程科学学报,2012,20(3):345-354.
[13]Wang X H,Zhang H M,Yang F L,et al.Improved stability and performance of aerobic granules under stepwise increased selection pressure[J].Enzyme and Microbial.Technology,2007,41(3):205-211.
[14]王盟,卞伟,侯爱月,等.两段式曝气工艺的短程硝化反硝化特性[J].化工学报,2016,67(4):1497-1504.
[15]石宪奎,王凯军,倪文.颗粒污泥粒径的工程测定方法[J].环境污染与防治,2006,28(2):140-142.
[16]王浩宇,苏本生,黄丹,等.好氧污泥颗粒化过程中Zeta电位与EPS的变化特性[J],环境科学,2012,33(5):1614-1620.
[17]Tay J H,Liu Q S,Liu Y.The effects of shear force on the formation,structure and metabolism of aerobic granules[J].Applied Microbiology And Biotechnology,2001,57(1/2):227-233.
[18]阮文权,卞庆荣,陈坚.COD与DO对AGS同步硝化反硝化脱氮的影响[J].应用与环境生物学报,2004,10(3):366-369.
[19]Nogueira R,Melo L F,Purkhold U,et al.Nitrifying and heterotrophic population dynamics in biofilm reactors:effects of hydraulic retention time and the presence of organic carbon[J].Water Res.,2002,36(2):469-481.
[20]李冬,姜沙沙,张金库,等.颗粒活性炭诱导亚硝化污泥快速颗粒化[J].中国环境科学,2016,36(1):50-55.
[21]王一波,蔡昌凤,黄礼超.活性炭粉末对AGS形成及性能的影响研究[J].安徽工程大学学报,2012,27(2):23-26.
[22]刘宏波,杨昌柱,濮文虹,等.进水氨氮浓度对AGS的影响研究[J].环境科学,2009,30(7):2030-2034.
[23]张亮,张树军,彭永臻.污水处理中游离氨对硝化作用抑制影响研究[J].哈尔滨工业大学学报,2012,44(2):75-79.
[24]Yan L,Hu H,Zhang S,et al.Arsenic tolerance and bioleaching fromrealgar based on response surface methodology by Acidithiobacillus ferrooxidans isolated from Wudalianchi volcanic lake,northeast China[J].Electronic Journal of Biotechnology,2017,25:50-57.
[25]王昌稳,李军,赵白航,等.AGS的快速培养与污泥特性分析[J].中南大学学报(自然科学版),2013,44(6):2623-2628.
[26]Liu Y,Yang S F,Liu Q S,et al.The role of cell hydrophobicity in the formation of aerobic granules[J].Curr.Microbiol.,2003,46(4):270-274.
[27]Tay J H,Liu Q S,Liu Y.Characteristics of aerobic granules grown on glucose and acetate in sequential aerobic sludge blanket reactors[J].Environmental Technology,2002,23(8):931-936.