虫草多糖对平阳霉素诱导的肺纤维化小鼠的影响
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摘要
目的研究虫草多糖抗小鼠肺纤维化的作用。方法采用气管注射平阳霉素制作小鼠肺纤维化模型,酶联免疫吸附法测定血清白介素1受体抑制剂(interleukin-1receptorantagonist,IL-1RA)含量,碱水解法测定肺组织中羟脯氨酸(hydroxyproline,Hyp)含量,Masson染色法观察肺组织胶原沉积情况。结果与肺纤维化模型组相比,虫草菌粉组、虫草总多糖组和虫草多糖[分子量(molecular mass,MW)<2×10~5]组血清中IL-1RA水平明显升高,肺组织中Hyp含量明显降低;虫草总多糖组和虫草多糖(MW<2×10~5)组肺组织纤维化面积明显减小。结论虫草多糖具有明显的抗小鼠肺纤维化作用。
OBJECTIVE To study the effects of cordyceps polysaccharide on pulmonary fibrosis model of mice.METHODS The pulmonary fibrosis model of mice was established by intratracheal injection of pingyangmycin. The content of interleukin-1 receptor antagonist(IL-1 RA) in serum was determined by ELISA, and the content of hydroxyproline(Hyp) in lung tissue was determined by alkali hydrolysis. Collagen deposition in lung tissue was observed by Masson staining. RESULTS Compared with the pulmonary fibrosis model group, the content of IL-1 RA in serum of fermentation cordyceps powder group,total cordyceps polysaccharide group and cordyceps polysaccharide group [molecular mass(MW)<2×10~5] evidently increased,but the content of Hyp in lung tissue evidently decreased. The fibrosis area of total cordyceps polysaccharide group and cordyceps polysaccharide group(MW<2×10~5) evidently decreased. CONCLUSION The cordyceps polysaccharide have significant anti-pulmonary fibrosis effects on model mice induced by pingyangmycin.
OBJECTIVE To study the effects of cordyceps polysaccharide on pulmonary fibrosis model of mice.METHODS The pulmonary fibrosis model of mice was established by intratracheal injection of pingyangmycin. The content of interleukin-1 receptor antagonist(IL-1 RA) in serum was determined by ELISA, and the content of hydroxyproline(Hyp) in lung tissue was determined by alkali hydrolysis. Collagen deposition in lung tissue was observed by Masson staining. RESULTS Compared with the pulmonary fibrosis model group, the content of IL-1 RA in serum of fermentation cordyceps powder group,total cordyceps polysaccharide group and cordyceps polysaccharide group [molecular mass(MW)<2×10~5] evidently increased,but the content of Hyp in lung tissue evidently decreased. The fibrosis area of total cordyceps polysaccharide group and cordyceps polysaccharide group(MW<2×10~5) evidently decreased. CONCLUSION The cordyceps polysaccharide have significant anti-pulmonary fibrosis effects on model mice induced by pingyangmycin.
引文
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[2]ZHENG M.Recovery,purification and biological activities of polysaccharide from cordyceps fermentation broth[D].Hangzhou:Zhejiang University of Technology,2009.
[3]ZHU P X.Study on the purification and physiological activity of cordyceps sinensis polysaccharide[D].Wuhan:Huazhong Agricultural University,2013.
[4]CHEN L,AN L G.Studies on the anti-tumour activity and immunomodulatory function of polysaccharide of cordyceps sinensis(CSP)[J].J Shandong Norm Univ(Nat Sci)[山东师范大学学报(自然科学版)],2013,30(10):109-112.
[5]GUO J.Separation and fermentation of cordyceps strains from Naqu and evaluation of immunological function for cordyceps polysaccharide[D].Baoding:Agricultural University of Hebei Province,2015.
[6]LI F H,LIU P,XIONG W G,et al.Effects of corydyceps polysaccharide on liver fibrosis induced by DMN in rats[J].China J Chin Mater Med(中国中药杂志),2006,31(23):1968-1971.
[7]PENG J H,LI X M,HU Y Y,et al.Effect of cordyceps polysaccharide on lipid peroxidation of rats with dimethylnitrosamine-induced liver fibrosis[J].China J Chin Mater Med(中国中药杂志),2013,38(3):391-396.
[8]HUANG X,LIU H X,LIU C,et al.Intervention effects of Xuefu Zhuyu Tang on oxidative stress and ECM metabolism in animals with pulmonary fibrosis[J].J Beijing Univ Tradit Chin Med(北京中医药大学学报),2012,35(11):752-756.
[9]吕晓东,庞立健.气管内注入博莱霉素致大鼠肺纤维化动物模型方法优化分析[J].实用中医内科杂志,2007,21(2):3-4.
[10]李京红,宋明臣.肺间质纤维化治疗的研究进展[J].国外医学呼吸系统分册,1998,18(1):18-20.
[11]付文华,田洁,张媛,等.百令胶囊预防放射性肺炎及肺纤维化临床观察[J].2012,20(10):174.
[12]马婧,袁维真.百令胶囊辅助治疗特发性肺纤维化临床疗效观察[J].北方药学,2013,10(12):25.
[13]LIU X J,YAO H Y,TU Y,et al.Effects of respiratory rehabilitation training combined with Corbrin Capsule on the inflammatory response and pulmonary fibrosis in patients with silicosis complicated by COPD[J].J Hainan Med Univ(海南医学院学报),2018,24(4):480-483.