麦芽糖假丝酵母降解苯酚和4-氯酚的特性
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摘要
考察麦芽糖假丝酵母对单底物4-氯酚的降解特性,研究苯酚与4-氯酚在双底物降解体系中的相互作用,并对比分析该菌株对苯酚和4-氯酚的降解能力。研究发现,麦芽糖假丝酵母能以4-氯酚为唯一碳源和能源,可在93 h内降解350 mg/L 4-氯酚,其降解4-氯酚的能力低于苯酚。酶活分析表明,4-氯酚降解遵循邻位裂解途径。细胞生长动力学过程符合Haldane方程,动力学参数为:μmax=0.252 h-1,Ks=33.58 mg/L,Ki=147.44 mg/L,相关系数R2=0.99。在苯酚/4-氯酚双底物降解体系中,较低浓度苯酚(200~400 mg/L)可以促进4-氯酚的降解,而较高浓度苯酚会产生抑制作用,当苯酚初始浓度为300 mg/L时,4-氯酚降解速率最大;4-氯酚的存在会抑制苯酚的降解。采用Abuhamed等人提出的动力学方程可以准确描述苯酚/4-氯酚双底物降解体系中细胞生长过程,得到两物质之间的相互抑制参数:I1,2=1.71,I2,1=3.25,相关系数R2=0.93。
Strain Candida maltosa was used to study the biodegradation characteristics of 4-chlorophenol as single substrate and the interaction between phenol and 4-chlorophenol in dual substrate biodegradation system.The capacities of the strain to degrade phenol and 4-chlorophenol were analyzed. The result showed that Candida maltosa could utilize 4-chlorophenol as the sole carbon and energy source and thoroughly biodegrade 4-chlorophenol up to 350 mg / L within 93 h. Its capacity to degrade 4-chlorophenol was obviously lower than that to degrade phenol. The enzyme assay showed that the degradation of 4-chlorophenol followed the pathway of ortho fission.Growth kinetics of the strain fitted the Haldane equation,and the parameters were: μmax= 0. 252 h- 1,Ks=33. 58 mg / L,Ki= 147. 44 mg / L with correlation coefficient R2= 0. 99. The presence of low-concentration phenol( 200-400 mg / L) could accelerate 4-chlorophenol degradation in the phenol /4-chlorophenol dual substrate degradation system. However,the high-concentration phenol inhibited the biodegradation of 4-chlorophenol. The degradation rate of 4-chlorophenol was fastest when the concentration of phenol was 300 mg / L. The presence of4-chlorophenol inhibited the biodegradation of phenol. The kinetics equation proposed by Abuhamed could adequately describe cell growth in binary mixture of phenol and 4-chlorophenol and the interaction parameters were obtained as follows: I1,2= 1. 71,I2,1= 3. 25 with correlation coefficient R2= 0. 93.
Strain Candida maltosa was used to study the biodegradation characteristics of 4-chlorophenol as single substrate and the interaction between phenol and 4-chlorophenol in dual substrate biodegradation system.The capacities of the strain to degrade phenol and 4-chlorophenol were analyzed. The result showed that Candida maltosa could utilize 4-chlorophenol as the sole carbon and energy source and thoroughly biodegrade 4-chlorophenol up to 350 mg / L within 93 h. Its capacity to degrade 4-chlorophenol was obviously lower than that to degrade phenol. The enzyme assay showed that the degradation of 4-chlorophenol followed the pathway of ortho fission.Growth kinetics of the strain fitted the Haldane equation,and the parameters were: μmax= 0. 252 h- 1,Ks=33. 58 mg / L,Ki= 147. 44 mg / L with correlation coefficient R2= 0. 99. The presence of low-concentration phenol( 200-400 mg / L) could accelerate 4-chlorophenol degradation in the phenol /4-chlorophenol dual substrate degradation system. However,the high-concentration phenol inhibited the biodegradation of 4-chlorophenol. The degradation rate of 4-chlorophenol was fastest when the concentration of phenol was 300 mg / L. The presence of4-chlorophenol inhibited the biodegradation of phenol. The kinetics equation proposed by Abuhamed could adequately describe cell growth in binary mixture of phenol and 4-chlorophenol and the interaction parameters were obtained as follows: I1,2= 1. 71,I2,1= 3. 25 with correlation coefficient R2= 0. 93.
引文
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[19]Bradford M.M.A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical Biochemistry,1976,72(1):248-254
[20]Higson F.K.,Focht D.D.Utilization of 3-chloro-2-methylbenzoic acid by Pseudomonas cepacia MB2 through the meta fission pathway.Applied and Environmental Microbiology,1992,58(8):2501-2504
[21]Solianikova I.P.,Maltseva O.V.,Vollmer M.D,et al.Characterization of muconate and chloromuconate cycloisomerase from Rhodococcus erythropolis 1CP:Indications for functionally convergent evolution among bacterial cycloisomerases.Journal of Bacteriology,1995,177(10):2821-2826
[22]戚以政,汪叔雄.生化反应动力学与反应器.北京:化学工业出版社,1992
[23]Abuhamed T.,Bayraktar E.,Mehmetolu T.,et al.Kinetics model for growth of Pseudomonas putida F1 during benzene,toluene and phenol biodegradation.Process Biochemistry,2004,39(8):983-988
[2]Wang Hui,Wang Jianlong.Electrochemical degradation of4-chlorophenol using a novel Pd/C gas-diffusion electrode.Applied Catalysis B:Environmental,2007,77(1-2):58-65
[3]葛启隆,岳秀萍,王国英.一株苯酚降解菌的分离鉴定及响应面法优化其固定化.中国环境科学,2014,34(2):518-525Ge Qilong,Yue Xiuping,Wang Guoying.Isolation and identification of a phenol-degrading strain and optimization for its immobilization using response surface methodology.China Environmental Science,2014,34(2):518-525(in Chinese)
[4]徐夫元,李星,陈英文,等.无机阴离子对Cu O-H2O2氧化苯酚废水的影响.环境科学,2009,30(2):451-456Xu Fuyuan,Li Xing,Chen Yingwen,et al.Influences of Inorganic anions on oxidation of phenol by Cu O-H2O2.Environmental Science,2009,30(2):451-456(in Chinese)
[5]张芳,李光明,盛怡,等.三维电解法处理苯酚废水的粒子电极研究.环境科学,2007,28(8):1715-1719Zhang Fang,Li Guangming,Sheng Yi,et al.Preparation of Particle-electrodes for treating phenol wastewater using Three-dimensional electrolysis.Environmental Science,2007,28(8):1715-1719(in Chinese)
[6]王姝,杨波,张婷婷,等.钯/泡沫镍对水体中4-氯酚的氢解脱氯研究.中国环境科学,2009,29(10):1065-1069Wang Shu,Yang Bo,Zhang Tingting,et al.Catalytic hydrodechlorination of 4-chlorophenol in aqueous solution by a palladized foam nickel catalyst.China Environmental Science,2009,29(10):1065-1069(in Chinese)
[7]Li Xiaoyan,Cui Yuhong,Feng Yujie,et al.Reaction pathways and mechanisms of the electrochemical degradation of phenol on different electrodes.Water Research,2005,39(10):1972-1981
[8]高超,王启山.吸附法处理含酚废水的研究进展.水处理技术,2011,37(1):1-4Gao Chao,Wang Qishan.Progresses of phenol wastewater based on adsorption method.Technology of Water Treatment,2011,37(1):1-4(in Chinese)
[9]张海涛.我国含酚废水处理技术研究进展.上海环境科学,2001,20(2):60-62Zhang Haitao.Study progress on treatment of wastewater containing hydroxybenzene in China.Shanghai Environmental Sciences,2001,20(2):60-62(in Chinese)
[10]Caizares P.,Lobato J.,Paz R.,et al.Electrochemical oxidation of phenolic wastes with boron-doped diamond anodes.Water Research,2005,39(12):2687-2703
[11]Belaid C.,Khadraoui M.,Mseddi S.,et al.Electrochemical treatment of olive mill wastewater:Treatment extent and effluent phenolic compounds monitoring using some uncommon analytical tools.Journal of Environmental Sciences,2013,25(1):220-230
[12]于萍,姚琳,罗运柏.高浓度含酚废水处理的新工艺.工业水处理,2002,22(9):5-8Yu Ping,Yao Lin,Luo Yunbai.Degradation treatment of highly concentrated phenol-containing industrial wastewater.Industrial Water Treatment,2002,22(9):5-8(in Chinese)
[13]石先阳,孙庆业.苯酚的厌氧生物处理.环境工程学报,2009,3(8):1409-1414Shi Xianyang,Sun Qingye.Anaerobic biological treatment of phenol.Chinese Journal of Environmental Engineering,2009,3(8):1409-1414(in Chinese)
[14]Saravanan P.,Pakshirajan K.,Saha P.Kinetics of phenol and m-cresol biodegradation by an indigenous mixed microbial culture isolated from a sewage treatment plant.Journal of Environmental Sciences,2008,20(12):1508-1513
[15]孙纪全,汤岳琴,刘伟强,等.两株假单胞菌降解酚类化合物的特性.中国环境科学,2010,30(12):1633-1638Sun Jiquan,Tang Yueqin,Liu Weiqiang,et al.Characteristics of degradation of phenolic compounds by two Pseudomonas strains.China Environmental Science,2010,30(12):1633-1638(in Chinese)
[16]宋正光,王国英,岳秀萍,等.麦芽糖假丝酵母降解苯酚的特性及动力学研究.中国给水排水,2013,29(9):77-80Song Zhengguang,Wang Guoying,Yue Xiuping,et al.Characteristics and Kinetics of Phenol Degradation by Candida maltosa.China Water&Wastewater,2013,29(9):77-80(in Chinese)
[17]国家环境保护总局.水和废水监测分析方法(第4版).北京:中国环境科学出版社,2002
[18]Bae H.S.,Lee J.M.,Kim Y.B.,et al.Biodegradation of the mixtures of 4-chlorophenol and phenol by Comamonas testosteroni CPW301.Biodegradation,1996,7(6):463-469
[19]Bradford M.M.A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical Biochemistry,1976,72(1):248-254
[20]Higson F.K.,Focht D.D.Utilization of 3-chloro-2-methylbenzoic acid by Pseudomonas cepacia MB2 through the meta fission pathway.Applied and Environmental Microbiology,1992,58(8):2501-2504
[21]Solianikova I.P.,Maltseva O.V.,Vollmer M.D,et al.Characterization of muconate and chloromuconate cycloisomerase from Rhodococcus erythropolis 1CP:Indications for functionally convergent evolution among bacterial cycloisomerases.Journal of Bacteriology,1995,177(10):2821-2826
[22]戚以政,汪叔雄.生化反应动力学与反应器.北京:化学工业出版社,1992
[23]Abuhamed T.,Bayraktar E.,Mehmetolu T.,et al.Kinetics model for growth of Pseudomonas putida F1 during benzene,toluene and phenol biodegradation.Process Biochemistry,2004,39(8):983-988