蜡状芽孢杆菌菌株SMC的间甲酚降解特性及动力学
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摘要
针对高浓度间甲酚废水难降解的问题,通过筛选获得一株高效降解间甲酚的菌株,并进一步探究其对间甲酚的降解特性和降解动力学。本文从某焦化厂活性污泥中经过驯化、筛选分离得到一株以间甲酚为唯一碳源的高效降解菌株,对其进行16S r DNA分子鉴定;通过测定培养液中间甲酚的剩余浓度,考察在菌株对间甲酚的最佳降解条件;并采用Haldane模型来模拟菌株降解间甲酚的动力学行为。将分离得到的菌株命名为SMC,初步鉴定为Bacillus cereus;在p H值为7.5、温度为30℃、摇床转速为150r/min的最佳培养条件下,菌株SMC在48h内对浓度1600mg/L的间甲酚降解率达97.81%,对间甲酚最大耐受能力为1900mg/L;经过Haldane模型拟合后的动力学参数为μmax=0.01252h-1,KS=34.58mg/L,Ki=479.5mg/L(R2=0.932)。菌株SMC对间甲酚有较强的降解能力,在高效处理含酚废水方面具有一定的应用前景。
A bacterial strain SMC was isolated from the aerobic sludge of coking wastewater to resolve the problem of difficulty in treating effluent with high concentration of m-cresol. The characteristics and kinetics of degradation of m-cresol by the strain was investigated with m-cresol as sole source of carbon in the cultural medium. After isolation and screening,16 S r DNA gene was analyzed to identify the taxonomic position of the strain. Its degradation capability under optimal growth conditions was studied by testing the concentration of m-cresol in residue medium. Haldane type substrate inhibit model was used to simulate cell growth on m-cresol. The isolated strain named as SMC belonged to Bacillus cereus. Under the optimal conditions of p H 7.5、30℃,150r/min of shaking rate,m-cresol with 1600mg/L initial concentration would be degraded 97.81% in 48 h and the highest tolerance to m-cresol was 1900mg/L. The kinetic parameters were:μmax=0.01252h-1,KS=34.58mg/L,Ki=479.5mg/L(R2 = 0.932). SMC could degrade m-cresol effectively,with good application potential for wastewater containing high concentration of m-cresol.
A bacterial strain SMC was isolated from the aerobic sludge of coking wastewater to resolve the problem of difficulty in treating effluent with high concentration of m-cresol. The characteristics and kinetics of degradation of m-cresol by the strain was investigated with m-cresol as sole source of carbon in the cultural medium. After isolation and screening,16 S r DNA gene was analyzed to identify the taxonomic position of the strain. Its degradation capability under optimal growth conditions was studied by testing the concentration of m-cresol in residue medium. Haldane type substrate inhibit model was used to simulate cell growth on m-cresol. The isolated strain named as SMC belonged to Bacillus cereus. Under the optimal conditions of p H 7.5、30℃,150r/min of shaking rate,m-cresol with 1600mg/L initial concentration would be degraded 97.81% in 48 h and the highest tolerance to m-cresol was 1900mg/L. The kinetic parameters were:μmax=0.01252h-1,KS=34.58mg/L,Ki=479.5mg/L(R2 = 0.932). SMC could degrade m-cresol effectively,with good application potential for wastewater containing high concentration of m-cresol.
引文
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[2]付柳,任源,韦朝海.间甲酚高效降解菌的筛选及其降解特性[J].化工进展,2008,27(7):1032-1037.
[3]Jiang Yan,Cai Xun,Wu Di,et al.Biodegradation of phenol and m-cresol by mutated Candida tropicalis[J].Journal of Environmental Sciences,2010,22(4):621-626.
[4]Pichiah Saravanan,Pakshirajan K,Prabirkumar Saha.Batch growth kinetics of an indigenous mixed microbial culture utilizing m-cresol as the sole carbon source[J].Journal of Hazardous Materials,2009,162:476-481.
[5]姚海艳.Lysinibacillus cresolivorans的间甲酚降解特性及动力学研究[D].广州:华南理工大学,2011.
[6]国家环境保护总局.水和废水监测分析方法[M].北京:中国环境科学出版社,2002.
[7]Yao Haiyan,Ren Yuan,Deng Xiuqiong,et al.Dual substrates biodegradation kinetics of m-cresol and pyridine by Lysinibacillus cresolivorans[J].Journal of Hazardous Materials,2011,186(2-3):1136-1140.
[8]宋正光,王国英,岳秀萍,等.麦芽糖假丝酵母菌降解苯酚和间甲酚的特性研究[J].中国给水排水,2013,21:97-99.
[9]白静.粪产碱杆菌降解苯酚、间甲酚的特性及其动力学研究[D].天津:天津大学,2006.
[10]Bai Jing,Wen Jianping,Li Hongmei,et al.Kinetic modeling of growth and biodegradation of phenol and m-cresol using Alcaligenes faecalis[J].Process Biochemistry,2007,42:510-517.
[11]彭丽花,任源,邓留杰,等.间甲酚降解菌Citrobacter farmeri的降解特性及代谢途径解析[J].环境化学,2009(1):44-48.
[12]吴坤,徐淑霞,闵航,等.间甲酚对杂色云芝(Coriolus versicolor)生长及产漆酶能力的影响[J].环境科学学报,2004,06:1135-1141.
[13]Arutchelvan V,Kanakasabai V,Elangovan R,et al.Kinetics of high strength phenol degradation using Bacillus brevis[J].Journal of Hazardous Materials,2006,129(1-3):216-222.
[14]袁利娟,姜立春,彭正松,等.高效降酚菌Bacillus sp.JY01的固定化及降解特性研究[J].环境科学与技术,2010,33(4):49-52,56.
[15]李淑彬,陈振军,丘李莉,等.蜡状芽孢杆菌菌株Jp-A的分离鉴定及其降解苯酚特性[J].应用生态学报,2006,17(5):920-924.
[16]于彩虹,陈飞,胡琳娜,等.一株苯酚降解菌的筛选及降解动力学特性[J].环境工程学报,2014(3):1215-1220.
[17]Jiang Yan,Wen Jianping,Bai Jing,et al.Phenol biodegradation by the yeast Candida tropicalis in the presence of m-cresol[J].Biochemical Engineering Journal,2006,29:227-234.
[18]Kampfer P,Rossello-Mora R,Scholz HC,et al.Description of Pseudochrobactrum gen.nov.,with the two species Pseudochrobactrum asaccharolyticum sp.Nov.and Pseudochrobactrum saccharolyticum sp.nov.[J].International Journal of Systematic and Evolutionary Microbiology,2006,56:1823-1829.