枯草芽孢杆菌对铅暴露鲫鱼肠道的影响
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摘要
为了研究枯草芽孢杆菌(WB600)对铅暴露鲫鱼肠道的影响,试验选取健康的鲫鱼192尾,随机分为8组,分别为对照Ⅰ组,枯草芽孢杆菌阳性对照Ⅱ组(1×109cfu/g),铅暴露Ⅲ组、Ⅴ组、Ⅶ组(0. 05,0. 5,1 mg/L),益生菌治疗Ⅳ组、Ⅵ组、Ⅷ组(0. 05 mg/L Pb+WB600、0. 5 mg/L Pb+WB600、1 mg/L Pb+WB600)。60 d后取肠道组织,通过常规方法制作石蜡切片,测量肠道绒毛高度、宽度及肌层厚度,从而评价该菌株对铅暴露鲫鱼肠道组织的影响。结果表明:与Ⅰ组相比,Ⅱ组近端肠和远端肠的绒毛高度、宽度、肌层厚度均显著增加(P <0. 05);与铅暴露组相比,益生菌治疗组近端肠及远端肠的绒毛高度、宽度、肌层厚度均不同水平增加;与Ⅲ组相比,Ⅳ组远端肠绒毛宽度显著增加(P<0. 05),其他均无显著差异(P>0. 05);与Ⅴ组相比,Ⅵ组近端肠绒毛宽度和远端肠绒毛高度、肌层厚度显著增加(P<0. 05),其他均无显著差异(P>0. 05);与Ⅶ组相比,Ⅷ组近端肠绒毛宽度和远端肠肌层厚度显著增加(P<0. 05),其他均无显著差异(P>0. 05)。说明枯草芽孢杆菌对不同铅浓度环境中鲫鱼的肠道结构有积极影响,对缓解铅导致的肠道损伤有一定的作用。
In order to investigate the effects of the Bacillus subtilise WB600 on the intestines of lead exposed crucian carp,192 healthy crucian carp were randomly divided into eight groups,respectively,which were contrast group Ⅰ,positive contrast group Ⅱ of Bacillus subtilis( 1 ×10~9 cfu/g),lead exposure group Ⅲ,Ⅴ and Ⅶ( 0. 05,0. 5,1 mg/L),probiotics treatment groups Ⅳ,Ⅵ and Ⅷ( 0. 05 mg/L Pb + WB600,0. 5 mg/L Pb+WB600,1 mg/L Pb+WB600). Sixty days later,intestine tissues were collected to test for making paraffin section by conventional method to measure the height,width and muscle thickness of intestinal villus,so as to evaluate the effects of the Bacillus subtilis WB600 on the intestines of lead exposed crucian carp. The results showed that compared with group Ⅰ,the height,width,muscle thickness of proximal and distal intestine villus of group Ⅱ were significantly increased( P<0. 05).Compared with lead exposure group,the height,width,muscle thickness of proximal and distal intestine villus of probiotics treatment groups were increased respectively. Compared with group Ⅲ,the width of distal intestinal villus of group Ⅳ were significantly increased( P<0. 05),the other had no significant difference( P> 0. 05).Compared with group Ⅴ,the width of the proximal intestinal villus and the height and muscle thickness of the distal intestinal villus of group Ⅵ were significantly increased( P<0. 05),the other had no significant difference( P> 0. 05). Compared with group Ⅶ,the width of the proximal intestinal villus and muscle thickness of the distal intestinal villus of group Ⅷ were significantly increased( P<0. 05),the other had no significant difference( P> 0. 05). Thus,the Bacillus subtilis WB600 has a positive effect on the intestinal structure of crucian carp in different lead concentrations,and plays a role in alleviating the intestinal damage caused by lead.
In order to investigate the effects of the Bacillus subtilise WB600 on the intestines of lead exposed crucian carp,192 healthy crucian carp were randomly divided into eight groups,respectively,which were contrast group Ⅰ,positive contrast group Ⅱ of Bacillus subtilis( 1 ×10~9 cfu/g),lead exposure group Ⅲ,Ⅴ and Ⅶ( 0. 05,0. 5,1 mg/L),probiotics treatment groups Ⅳ,Ⅵ and Ⅷ( 0. 05 mg/L Pb + WB600,0. 5 mg/L Pb+WB600,1 mg/L Pb+WB600). Sixty days later,intestine tissues were collected to test for making paraffin section by conventional method to measure the height,width and muscle thickness of intestinal villus,so as to evaluate the effects of the Bacillus subtilis WB600 on the intestines of lead exposed crucian carp. The results showed that compared with group Ⅰ,the height,width,muscle thickness of proximal and distal intestine villus of group Ⅱ were significantly increased( P<0. 05).Compared with lead exposure group,the height,width,muscle thickness of proximal and distal intestine villus of probiotics treatment groups were increased respectively. Compared with group Ⅲ,the width of distal intestinal villus of group Ⅳ were significantly increased( P<0. 05),the other had no significant difference( P> 0. 05).Compared with group Ⅴ,the width of the proximal intestinal villus and the height and muscle thickness of the distal intestinal villus of group Ⅵ were significantly increased( P<0. 05),the other had no significant difference( P> 0. 05). Compared with group Ⅶ,the width of the proximal intestinal villus and muscle thickness of the distal intestinal villus of group Ⅷ were significantly increased( P<0. 05),the other had no significant difference( P> 0. 05). Thus,the Bacillus subtilis WB600 has a positive effect on the intestinal structure of crucian carp in different lead concentrations,and plays a role in alleviating the intestinal damage caused by lead.
引文
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[16]邱燕.三种微生态制剂对草鱼(Ctenopharyngodon idellus)生长性能、生理机能及肠道黏膜的影响[D].苏州:苏州大学,2010.
[17]黄芳,刘文惠,吴轶,等.绿原酸缓解镉暴露致大鼠肠道损伤[EB/OL].(2017-06-28)[2018-03-04].http://kns.cnki.net/kcms/Detail/11.2206.TS.20170628.1628.030.html.
[2]吴波,李薇,付玉梅,等.重金属镉对参环毛蚓胃肠道上皮细胞超微结构损伤的研究[J].中药材,2011,34(12):1833-1837.
[3]YURONG Y,RUIPING S,SHIMIN Z,et al.Effect of probiotics on intestinal mucosal immunity and ultrastructure of cecal tonsils of chickens[J].Arch Anim Nutr,2005,59(4):237-246.
[4]OZTURK E,SAHIN M,CAN F,et al.P088 Effects of probiotics on systemic and intestinal mucosal immunity in splenectomized rats[J].Clin Nutr,2008,3(3):66.
[5]GERAYLOU Z,SOUFFREAU C,RURANGWA E,et al.Effects of dietary arabinoxylan-oligosaccharides(AXOS)and endogenous probiotics on the growth performance,non-specific immunity and gut microbiota of juvenile Siberian sturgeon(Acipenserbaerii)[J].Fish Shellfish Immunol,2013,35(3):766-775.
[6]BECK B R,KIM D,JEON J,et al.The effects of combined dietary probiotics Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 on innate immunity and disease resistance in olive flounder(Paralichthys olivaceus)[J].Fish Shellfish Immunol,2015,42(1):177-183.
[7]JOHNSONHENRT K C,DONATO K A,SHENTU G,et al.Lactobacillus rhamnosus strain GG prevents enterohemorrhagic escherichia coli O157:H7-Induced changes in epithelial barrier function[J].Infect Immun,2008,76(4):1340-1348.
[8]BUBNOV R V,BABENKO L P,LAZARENKO L M,et al.Comparative study of probiotic effects of Lactobacillus,and Bifidobacteria,strains on cholesterol levels,liver morphology and the gut microbiota in obese mice[J].Epma J,2017,8(4):357-376.
[9]PARK S,JI Y,JUNG H Y,et al.Lactobacillus plantarum HAC01regulates gut microbiota and adipose tissue accumulation in a dietinduced obesity murine model[J].Appl Microbiol Biotechnol,2017,101(4):1605-1614.
[10]高欣,盖力强,李美英,等.芽孢杆菌对西伯利亚鲟幼鱼摄食生长和消化率的影响[J].河北师范大学学报(自然科学版),2009,33(3):377-382.
[11]沈斌乾,陈建明,郭建林,等.饲料中添加枯草芽孢杆菌对青鱼生长、消化酶活性和鱼体组成的影响[J].水生生物学报,2013,37(1):48-53.
[12]仵天培,夏中生,黄建烨,等.中国畜牧兽医学会动物营养学分会第十一次全国动物营养学术研讨会论文集[C].长沙:中国畜牧兽医学会,2017.
[13]程远,黄凯,黄秀芸,等.饲料中添加枯草芽孢杆菌对吉富罗非鱼幼鱼生长性能、免疫力和抗氧化功能的影响[J].动物营养学报,2014,26(6):1503-1512.
[14]潘康成,何明清,刘克琳.微生物添加剂对鲤鱼生长和消化酶活性的影响研究[J].饲料工业,1997,18(10):41-42.
[15]孙盛明,苏艳莉,张武肖,等.饲料中添加枯草芽孢杆菌对团头鲂幼鱼生长性能、肝脏抗氧化指标、肠道菌群结构和抗病力的影响[J].动物营养学报,2016,28(2):507-514.
[16]邱燕.三种微生态制剂对草鱼(Ctenopharyngodon idellus)生长性能、生理机能及肠道黏膜的影响[D].苏州:苏州大学,2010.
[17]黄芳,刘文惠,吴轶,等.绿原酸缓解镉暴露致大鼠肠道损伤[EB/OL].(2017-06-28)[2018-03-04].http://kns.cnki.net/kcms/Detail/11.2206.TS.20170628.1628.030.html.