饥饿和重摄食对鲤鱼脂蛋白脂肪酶活性的影响
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摘要
采用对硝基苯酚法测定正常摄食、饥饿(14 d)和重摄食(3 d)状态下鲤鱼不同组织和器官中脂肪酶及脂蛋白脂肪酶(lipoproteinlipase,LPL)的活性。结果显示:与正常摄食相比,饥饿鲤鱼肌肉、脂肪和心脏中脂肪酶比活力显著下降(P<0.05),但脂肪和心脏中LPL比活力显著升高(P<0.05);除脂肪中LPL外,重摄食3d的鲤鱼各组织和器官中脂肪酶和LPL比活力比正常摄食和饥饿鲤鱼均显著升高(P<0.05),有明显的补偿作用。
In this study, p-nitrophenol method was used to determine the activities of lipase and lipoprotein lipase(LPL) from different tissues and organs in normal feeding, starving for 14 days, and re-feeding for 3 days of Cyprinus carpio. The results showed that the activities of lipase from muscle, fat, and heart in starving group were significant decreased(P<0.05), while the activities of LPL from fat and heart in starving group were higher(P<0.05) than that of normal feeding group. Except for LPL from adipose, the activities of lipase and LPL in each tissues and organs of Cyprinus carpio were significant increased(P<0.05) at 3 days after refeeding, and displays obvious compensatory effect.
In this study, p-nitrophenol method was used to determine the activities of lipase and lipoprotein lipase(LPL) from different tissues and organs in normal feeding, starving for 14 days, and re-feeding for 3 days of Cyprinus carpio. The results showed that the activities of lipase from muscle, fat, and heart in starving group were significant decreased(P<0.05), while the activities of LPL from fat and heart in starving group were higher(P<0.05) than that of normal feeding group. Except for LPL from adipose, the activities of lipase and LPL in each tissues and organs of Cyprinus carpio were significant increased(P<0.05) at 3 days after refeeding, and displays obvious compensatory effect.
引文
[1]覃川杰,邵婷,杨洁萍,等.饥饿胁迫对瓦氏黄颡鱼脂肪代谢的影响[J].水生生物学报,2015,39(1):58-65.
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[14]胡阿凤.D-山梨糖对脂肪酶热稳定性的影响及其在酶精练中的应用[D].上海:东华大学,2012.
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[18]高露姣,陈立侨,赵晓勤,等.施氏鲟幼鱼的饥饿和补偿生长研究--对消化器官结构和酶活性的影响[J].中国水产科学,2004,11(5):413-419.
[19]杨代勤,陈芳,阮国良,等.饥饿对黄鳝消化酶活性的影响[J].应用生态学报,2007,18(5):1167-1170.
[20]李芹,唐洪玉,郑永华,等.饥饿和再投喂对厚颌鲂稚鱼生长及消化酶活性的影响[J].西南大学学报(自然科学版),2013,35(7):39-44.
[21]闫雪梅,谷江稳,郭晓鸽,等.饥饿银鲳幼鱼的能量利用规律及其生存策略[J].动物营养学报,2015,27(12):3975-3983.
[22]曹香林,郭蓓,彭墨,等.不同发育阶段草鱼消化酶活力的变化及其昼夜节律[J].河南农业科学,2009,38(7):120-123.
[23]王晓娟.日本鳗鲡对饥饿和恢复投喂的生理响应及其调节机制[D].厦门:厦门大学,2010.
[24]OKU H,KOIZUMI N,OKUMURA T,et al.Molecular characterization of lipoprotein lipase,hepatic lipase and pancreatic lipase genes:effects of fasting and refeeding on their gene expression in red sea bream Pagrus major[J].Comparative Biochemistry&Physiology Part B:Biochemistry&Molecular Biology,2006,145(2):168-178.
[25]区又君,刘泽伟.饥饿和再投喂对千年笛鲷幼鱼消化酶活性的影响[J].海洋学报(中文版),2007,29(1):86-91.
[26]苏艳莉,戈贤平,孙盛明,等.高温季节饥饿后补偿摄食对团头鲂幼鱼生长性能、血清生化指标、肠道消化酶活性及肝脏抗氧化酶活性的影响[J].动物营养学报,2017,29(11):4198-4206.
[27]钱云霞.饥饿对养殖鲈蛋白酶活力的影响[J].水产科学,2002,21(3):6-7.
[2]CHAIYACHATE R,YUANGSOI B,CHAMPASRI T,et al.Metabolic fuel level and enzyme activity changes in starved Broadhead catfish(Clarias macrocephalus Gunther,1864)[J].Bioscience Research,2018,15(3):1503-1510.
[3]曾令清,彭韩柳依,王健伟,等.南方鲇幼鱼运动代谢的个体变异和重复性及其饥饿响应[J].水产学报,2015,39(1):52-64.
[4]倪寿文,桂远明,刘焕亮.草鱼、鲤、鲢、鳙和尼罗非鲫脂肪酶活性的比较研究[J].大连水产学院学报,1990,5(3/4):19-24.
[5]吴婷婷,朱晓鸣.鳜鱼、青鱼、草鱼、鲤、鲫、鲢消化酶活性的研究[J].中国水产科学,1994,1(2):10-17.
[6]黎军胜,李建林,吴婷婷.奥尼罗非鱼淀粉酶、脂肪酶的分布与特性[J].中国水产科学,2004,11(5):473-477.
[7]BLACK D,SKINNER E R.Features of the lipid transport system of fish as demonstrated by studies on starvation in the rainbow trout[J].Journal of Comparative Physiology B:Biochemical Systematic and Environmental Physiology,1986,156(4):497-502.
[8]HAN C,WEN X,ZHENG Q,et al.Effect of starvation on activities and mRNA expression of lipoprotein lipase and hormone-sensitive lipase in tilapia(Oreochromis niloticus×O.areus)[J].Fish Physiology&Biochemistry,2011,37(1):113-122.
[9]HUANG H L,ZHANG Y,CAO M Y,et al.Effects of fasting on the activities and m RNA expression levels of lipoprotein lipase(LPL),hormone-sensitive lipase(HSL)and fatty acid synthetase(FAS)in spotted seabass Lateolabrax maculatus[J].Fish Physiology&Biochemistry,2018,44(1):387-400.
[10]SMITH P K,KROHN R I,HERMANSON G T,et al.Measurement of protein using bicinchoninic acid[J].Analytical Biochemistry,1985,150(1):76-85.
[11]周杰,俞菊华,李红霞,等.鲤鱼2种脂蛋白脂肪酶基因的c DNA克隆、表达及其酶活性[J].江苏农业学报,2016,32(2):414-423.
[12]QUIG D W,LAYMAN D K,BECHTEL P J,et al.The influence of starvation and refeeding on the lipoprotein lipase activity of skeletal muscle and adipose tissue of lean and obese Zucker rats[J].The Journal of Nutrition,1983,113(6):1150-1156.
[13]王乐,刘松,尹艳丽,等.一株酸性脂肪酶高产菌株的筛选与鉴定[J].食品安全质量检测学报,2017,8(12):4509-4515.
[14]胡阿凤.D-山梨糖对脂肪酶热稳定性的影响及其在酶精练中的应用[D].上海:东华大学,2012.
[15]王欢,何腊平,周换景,等.脂肪酶活力测定方法及其在筛选产脂肪酶微生物中的应用[J].生物技术通报,2013(1):203-208.
[16]GRINBERG D R,RAMíREZ I,VILARóS.Starvation enhances lipoprotein lipase activity in the liver of the newborn rat[J].Biochimica et Biophysica Acta(BBA)-Lipids and Lipid Metabolism,1985,833(2):217-222.
[17]VILARóS,TESTAR X,RAMíREZ I,et al.Lipoprotein lipase activity in the liver of starved pregnant rats[J].Neonatology,1990,57(1):37-45.
[18]高露姣,陈立侨,赵晓勤,等.施氏鲟幼鱼的饥饿和补偿生长研究--对消化器官结构和酶活性的影响[J].中国水产科学,2004,11(5):413-419.
[19]杨代勤,陈芳,阮国良,等.饥饿对黄鳝消化酶活性的影响[J].应用生态学报,2007,18(5):1167-1170.
[20]李芹,唐洪玉,郑永华,等.饥饿和再投喂对厚颌鲂稚鱼生长及消化酶活性的影响[J].西南大学学报(自然科学版),2013,35(7):39-44.
[21]闫雪梅,谷江稳,郭晓鸽,等.饥饿银鲳幼鱼的能量利用规律及其生存策略[J].动物营养学报,2015,27(12):3975-3983.
[22]曹香林,郭蓓,彭墨,等.不同发育阶段草鱼消化酶活力的变化及其昼夜节律[J].河南农业科学,2009,38(7):120-123.
[23]王晓娟.日本鳗鲡对饥饿和恢复投喂的生理响应及其调节机制[D].厦门:厦门大学,2010.
[24]OKU H,KOIZUMI N,OKUMURA T,et al.Molecular characterization of lipoprotein lipase,hepatic lipase and pancreatic lipase genes:effects of fasting and refeeding on their gene expression in red sea bream Pagrus major[J].Comparative Biochemistry&Physiology Part B:Biochemistry&Molecular Biology,2006,145(2):168-178.
[25]区又君,刘泽伟.饥饿和再投喂对千年笛鲷幼鱼消化酶活性的影响[J].海洋学报(中文版),2007,29(1):86-91.
[26]苏艳莉,戈贤平,孙盛明,等.高温季节饥饿后补偿摄食对团头鲂幼鱼生长性能、血清生化指标、肠道消化酶活性及肝脏抗氧化酶活性的影响[J].动物营养学报,2017,29(11):4198-4206.
[27]钱云霞.饥饿对养殖鲈蛋白酶活力的影响[J].水产科学,2002,21(3):6-7.
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