农户保存与种质库保存的同近名地方稻品种的遗传多样性研究（英文）
|
摘要
通过对湖南农户自留种原地保存(简称农户保存)与异地低温保存在湖南种质库(简称种质库保存)的92份同近名水稻材料(7组,同近名材料6组)的表现型及基因型进行分析,鉴定同近名品种间遗传距离,为之后有效保存、针对性供种、高效利用提供理论依据。结果表明,供试同近名材料间,及农户保存与种质库保存的同近名材料间在表现型性状上都有极显著差异。通过SSR标记基因型比较农户保存与种质库保存湖南同近名材料,发现除E组外,其他组都是农户保存的等位基因变异数小于种质库保存的等位基因变异数,说明农户保存的材料在年复一年的种植、收种过程中经历了自然选择和人工选择留种,进行了加代纯化。除C、E组外,在同近名组内SSR标记遗传相似系数显示,农户保存的材料间>种质库保存的材料间>农户保存材料与种质库保存材料,表明农户自留种保存的同近名水稻资源值得收集评价。
This study was conducted to analyze the phenotypic and genotypic similarity or diversity of rice(Oryza sativa L.) landraces in household and hypothermic genebank conservations in Hunan Province and identify the genetic distance between the rice landraces with the same or similar names. A total of 92 accessions of rice landraces were divided into seven groups according to their local names, but the morphological traits showed large variations even within the same group as well as between household and genebank conservations. The SSR marker analysis showed that, in all the groups except Group E, the allelic variations within each subgroup of the household conservation were less than those of the genebank conservation, indicating the household-conserved rice landraces' many-generation purification from natural and artificial selections in the process of their cultivation year after year; and the similarity coefficient among the household rice landraces was the highest except for Groups C and E. Thus, the study suggested that the same-name or similar-name rice resources retained by farmers would be valuable for collection and evaluation.
This study was conducted to analyze the phenotypic and genotypic similarity or diversity of rice(Oryza sativa L.) landraces in household and hypothermic genebank conservations in Hunan Province and identify the genetic distance between the rice landraces with the same or similar names. A total of 92 accessions of rice landraces were divided into seven groups according to their local names, but the morphological traits showed large variations even within the same group as well as between household and genebank conservations. The SSR marker analysis showed that, in all the groups except Group E, the allelic variations within each subgroup of the household conservation were less than those of the genebank conservation, indicating the household-conserved rice landraces' many-generation purification from natural and artificial selections in the process of their cultivation year after year; and the similarity coefficient among the household rice landraces was the highest except for Groups C and E. Thus, the study suggested that the same-name or similar-name rice resources retained by farmers would be valuable for collection and evaluation.
引文
[1]SUN G Z(孙桂芝).Classification and genetic similarity analysis of Hunan rice landraces(湖南稻种资源分类及遗传性状多样性分析).Crop Research(作物研究),1990,4(4):12-18.
[2]DUAN Y H(段永红),LI X X(李小湘),LIU W Q(刘文强),et al.Main features and applications of Hunan rice landraces(湖南稻种资源主要特征特性与利用状况).Journal of Plant Genetic Resources(植物遗传资源学报),2013,14(6):1059-1063.
[3]AHMED M S,AKTER K,RHSHID E,et al.Diversity analysis in Boro rice(Oryza sativa L.)accessions.Bangladesh Journal Agriculture Resources,2010,35(1):29-36.
[4]BRONDANI C,BORBA T C O,RANGEL P H N,et al.Determination of genetic variability of traditional varieties of Brazilian rice using microsatellite Markers.Genetics and Molecular Biology,2006,29(4):676-684.
[5]ZHU M Y,WANG Y Y,ZHU Y Y,et al.Estimating genetic diversity of rice landraces from yunnan by SSR assay and its implication for Conservation.Acta Botanica Sinica,2004,46(12):1458-1467.
[6]THOMSON M J,POLATO N R,PRASETIYONO J,et al.Genetic diversity of isolated populations of indonesian landraces of rice(Oryza sativa L.)collected in east Kalimantan on the island of Borneo.Rice,2009,2(1):80-92.
[7]LI X X(李小湘),XIAO J Z(肖军治),DUAN Y H(段永红),et al.Genetic similarity of hunan landraces rice with the same or similar name based on SSR Markers and phenotypic traits(湖南同名地方稻资源SSR标记及表现型的比较分析).Journal of Plant Genetic Resources(植物遗传资源学报),2014,15(2):248-254.
[8]WONG S C,YIU P H,BONG S T W,et al.Analysis of sarawak bario rice diversity using microsatellite markers.American Journal of Agricutural and Biological Sciences,2009,4(4):298-304.
[9]HAN L Z(韩龙植),WEI X H(魏兴华).Descriptors and data standard for rice(Oryza sativa L.)(水稻种质资源描述规范和数据标准).Beijing:China Agriculture Press(in Chinese)(北京:中国农业出版社),2006:1-119.
[10]LU Y J(卢扬江),ZHENG K L(郑康乐).A simple method for isolation of rice DNA(提取水稻DNA的一种简易方法).Chinese Journal Rice Science(中国水稻科学),1992,6(1):47-48.
[11]ZHAO Y Z(赵一洲),NI S J(倪善君),ZHANG Z(张战),et al.Analysis of quality character and relationship variation of rice from Liaoning Province(辽宁省水稻品种品质性状及亲缘关系变化分析).Journal of Plant Genetic Resource(植物遗传资源学报),2017,18(6):1039-1045.
[12]JIN W D(金伟栋),HONG D L(洪德林).Genetic diversity in japonica rice landraces(Orayza sativa)from the Taihu Lake region(太湖流域粳稻地方品种遗传多样性研究).Biodiversity Science(生物多样性),2006,14(6):479-487.
[13]XU F R(徐福荣),DONG C(董超),YANG W Y(杨文毅),et al.Genetic similarity based on SSR Markers and phenotypies traits of major improved rice(Orayza sativa L.)varieties in Yunnan Province(基于表型性状和SSR分子标记的云南省水稻主要育成品种(系)的遗传相似性分析).Journal of Plant Genetic Resource(植物遗传资源学报),2011,12(5):700-708.
[14]ZHANG D L(张冬玲),ZHANG H L(张洪亮),WEI X H(魏兴华),et al.Genetic structure and diversity of rice in Guizhou Province(贵州栽培稻的遗传结构及其遗传多样性).Chinese Science Bulletin(科学通报),2006,51(23):2747-2754.
[15]LI H Y(李红宇),HOU Y M(侯昱铭),CHEN Y H(陈英华),et al.Evaluation on genetic diversity of the commercial rice varieties in northeast China by Microsatellite Markers(用SSR标记评估东北三省水稻推广品种的遗传多样性).Chinese Journal Rice Science(中国水稻科学),2009,23(4):383-390.
[16]QI Y W(齐永文),ZHANG D L(张冬玲),ZHANG H L(张洪亮),et al.Genetic diversity of breeding rice varieties in China and its variation trend in recent 50 years(中国水稻选育品种遗传多样性及其近50年变化趋势).Chinese Science Bulletin(科学通报),2006,51(6):693-699.
[2]DUAN Y H(段永红),LI X X(李小湘),LIU W Q(刘文强),et al.Main features and applications of Hunan rice landraces(湖南稻种资源主要特征特性与利用状况).Journal of Plant Genetic Resources(植物遗传资源学报),2013,14(6):1059-1063.
[3]AHMED M S,AKTER K,RHSHID E,et al.Diversity analysis in Boro rice(Oryza sativa L.)accessions.Bangladesh Journal Agriculture Resources,2010,35(1):29-36.
[4]BRONDANI C,BORBA T C O,RANGEL P H N,et al.Determination of genetic variability of traditional varieties of Brazilian rice using microsatellite Markers.Genetics and Molecular Biology,2006,29(4):676-684.
[5]ZHU M Y,WANG Y Y,ZHU Y Y,et al.Estimating genetic diversity of rice landraces from yunnan by SSR assay and its implication for Conservation.Acta Botanica Sinica,2004,46(12):1458-1467.
[6]THOMSON M J,POLATO N R,PRASETIYONO J,et al.Genetic diversity of isolated populations of indonesian landraces of rice(Oryza sativa L.)collected in east Kalimantan on the island of Borneo.Rice,2009,2(1):80-92.
[7]LI X X(李小湘),XIAO J Z(肖军治),DUAN Y H(段永红),et al.Genetic similarity of hunan landraces rice with the same or similar name based on SSR Markers and phenotypic traits(湖南同名地方稻资源SSR标记及表现型的比较分析).Journal of Plant Genetic Resources(植物遗传资源学报),2014,15(2):248-254.
[8]WONG S C,YIU P H,BONG S T W,et al.Analysis of sarawak bario rice diversity using microsatellite markers.American Journal of Agricutural and Biological Sciences,2009,4(4):298-304.
[9]HAN L Z(韩龙植),WEI X H(魏兴华).Descriptors and data standard for rice(Oryza sativa L.)(水稻种质资源描述规范和数据标准).Beijing:China Agriculture Press(in Chinese)(北京:中国农业出版社),2006:1-119.
[10]LU Y J(卢扬江),ZHENG K L(郑康乐).A simple method for isolation of rice DNA(提取水稻DNA的一种简易方法).Chinese Journal Rice Science(中国水稻科学),1992,6(1):47-48.
[11]ZHAO Y Z(赵一洲),NI S J(倪善君),ZHANG Z(张战),et al.Analysis of quality character and relationship variation of rice from Liaoning Province(辽宁省水稻品种品质性状及亲缘关系变化分析).Journal of Plant Genetic Resource(植物遗传资源学报),2017,18(6):1039-1045.
[12]JIN W D(金伟栋),HONG D L(洪德林).Genetic diversity in japonica rice landraces(Orayza sativa)from the Taihu Lake region(太湖流域粳稻地方品种遗传多样性研究).Biodiversity Science(生物多样性),2006,14(6):479-487.
[13]XU F R(徐福荣),DONG C(董超),YANG W Y(杨文毅),et al.Genetic similarity based on SSR Markers and phenotypies traits of major improved rice(Orayza sativa L.)varieties in Yunnan Province(基于表型性状和SSR分子标记的云南省水稻主要育成品种(系)的遗传相似性分析).Journal of Plant Genetic Resource(植物遗传资源学报),2011,12(5):700-708.
[14]ZHANG D L(张冬玲),ZHANG H L(张洪亮),WEI X H(魏兴华),et al.Genetic structure and diversity of rice in Guizhou Province(贵州栽培稻的遗传结构及其遗传多样性).Chinese Science Bulletin(科学通报),2006,51(23):2747-2754.
[15]LI H Y(李红宇),HOU Y M(侯昱铭),CHEN Y H(陈英华),et al.Evaluation on genetic diversity of the commercial rice varieties in northeast China by Microsatellite Markers(用SSR标记评估东北三省水稻推广品种的遗传多样性).Chinese Journal Rice Science(中国水稻科学),2009,23(4):383-390.
[16]QI Y W(齐永文),ZHANG D L(张冬玲),ZHANG H L(张洪亮),et al.Genetic diversity of breeding rice varieties in China and its variation trend in recent 50 years(中国水稻选育品种遗传多样性及其近50年变化趋势).Chinese Science Bulletin(科学通报),2006,51(6):693-699.