典型抗生素复合污染对小白菜生长发育的毒理效应
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摘要
采用盆栽试验研究了土壤外源四环素与金霉素(TC-CTC)、四环素与磺胺嘧啶(TC-SD)二元复合污染对小白菜幼苗生长发育的毒理效应。结果表明,0.5 mg·kg~(-1)抗生素复合污染胁迫促进了小白菜芽长和根长的生长,但随着抗生素浓度的增加,小白菜芽长及根长生长逐渐受到抑制,根长受抑制程度高于芽长,25 mg·kg~(-1)TC-CTC处理对根长的抑制率达72.7%。与抗生素单一污染相比,二元复合污染胁迫使小白菜体内可溶性蛋白含量显著降低,暴露30 d时25 mg·kg~(-1)TC-CTC处理对可溶性蛋白抑制程度最大,抑制率为67.1%,同时超氧化物歧化酶、过氧化物酶和过氧化氢酶活性受到的抑制作用更显著,呈现协同作用。
We investigated the combined toxicological effects of tetracycline + chlortetracycline( TC-CTC) and tetracycline + sulfadiazine( TC-SD) on the growth and development of Chinese white cabbage seedlings under pot-culture. The results showed that the growth of shoots and roots of cabbage were promoted by the TC-CTC and TC-SD at a concentration of 0.5 mg·kg~(-1). With increasing concentration,the growth of shoots and roots were gradually inhibited. The inhibitive effects on root length were higher than that on shoot length. The inhibition rate of root length was up to 72.7% by TC-CTC at 25 mg·kg~(-1). Compared to single pollutant,TC-CTC and TC-SD significantly decreased the content of soluble protein in cabbage. The maximum inhibition rate of67.1% was presented in the treatment of TC-CTC at 25 mg·kg~(-1)after 30 days. Moreover,the activities of superoxide dismutase,peroxidase and catalase were inhibited more under such treatment,highlighting a synergistic effect.
We investigated the combined toxicological effects of tetracycline + chlortetracycline( TC-CTC) and tetracycline + sulfadiazine( TC-SD) on the growth and development of Chinese white cabbage seedlings under pot-culture. The results showed that the growth of shoots and roots of cabbage were promoted by the TC-CTC and TC-SD at a concentration of 0.5 mg·kg~(-1). With increasing concentration,the growth of shoots and roots were gradually inhibited. The inhibitive effects on root length were higher than that on shoot length. The inhibition rate of root length was up to 72.7% by TC-CTC at 25 mg·kg~(-1). Compared to single pollutant,TC-CTC and TC-SD significantly decreased the content of soluble protein in cabbage. The maximum inhibition rate of67.1% was presented in the treatment of TC-CTC at 25 mg·kg~(-1)after 30 days. Moreover,the activities of superoxide dismutase,peroxidase and catalase were inhibited more under such treatment,highlighting a synergistic effect.
引文
丛永平,姜蕾,王婷,等.2013.典型抗生素二元混合物对明亮发光杆菌的急性联合毒性.环境化学,32(7):1348-1352.
高礼,石丽娟,袁涛.2013.典型抗生素对羊角月牙藻的生长抑制及其联合毒性.环境与健康杂志,30(6):475-478.
金彩霞,陈秋颖,刘军军,等.2009.两种常用兽药对作物发芽的生态毒性效应.环境科学学报,29(3):619-625.
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律泽,胡筱敏,安婧,等.2014.佳乐麝香和镉复合污染对土壤中放线菌数量的影响.生态学杂志,33(6):1501-1507.
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赵英永,戴云,崔秀明,等.2006.考马斯亮蓝G-250染色法测定草乌中可溶性蛋白质含量.云南民族大学学报:自然科学版,15(3):235-237.
周启星,孔繁祥,朱琳.2004.生态毒理学.北京:科学出版社.
朱峰,苏丹,安婧,等.2017.磺胺类抗生素在土壤-植物系统中的迁移特征.生态学杂志,36(5):1402-1407
An J,Chen HW,Wei SH,et al.2015.Antibiotic contamination in animal manure,soil,and sewage sludge in Shenyang,northeast China.Environmental Earth Sciences,74:5077-5086.
An J,Zhou QX,Sun YB,et al.2009.Ecotoxicological effects of typical personal care products on seed germination and seedling development of wheat(Triticum aestivum).Chemosphere,76:1428-1434.
Broderius SJ,Kahl MD,Elonen GE,et al.2005.A comparison of the lethal and sublethal toxicity of organic chemical mixtures to the fathead minnow(Pimephales promelas).Environmental Toxicology&Chemistry,24:3117-3127.
Cleuvers M.2003.Aquatic ecotoxicity of pharmaceuticals including the assessment of combination effects.Toxicology Letters,142:185-194.
Hu XG,Zhou QX,Luo Y.2010.Occurrence and source analysis of typical veterinary antibiotics in manure,soil,vegetables and groundwater from organic vegetable bases,northern China.Environmental Pollution,158:2992-2998.
Migliore L,Godeas F,Filippis SP,et al.2010.Hormetic effect(s)of tetracyclines as environmental contaminant on Zea mays.Environmental Pollution,158:129-134.
Wu YX,Tiedemann AV.2002.Impact of fungicides on active oxygen species and antioxidant enzymes in spring barley(Hordeum vulgare L.)exposed to ozone.Environmental Pollution,116:37-47.
Zhang QQ,Ying GG,Pan CG,et al.2015.Comprehensive evaluation of antibiotics emission and fate in the river basins of China:Source analysis,multimedia modeling,and linkage to bacterial resistance.Environmental Science&Technology,49:6772-6782.
Zikic RV,Stajn A,Saicic ZS,et al.1996.The activities of superoxide dismutase,catalase and ascorbic acid content in the liver of goldfish(Carassius auratus gibelio Bloch.)exposed to cadmium.Physiological Research,45:479-481.
高礼,石丽娟,袁涛.2013.典型抗生素对羊角月牙藻的生长抑制及其联合毒性.环境与健康杂志,30(6):475-478.
金彩霞,陈秋颖,刘军军,等.2009.两种常用兽药对作物发芽的生态毒性效应.环境科学学报,29(3):619-625.
孔祥生,易现峰.2008.植物生理学实验技术.北京:中国农业出版社.
林琳,安婧,周启星.2011.土壤四环素污染对小白菜幼苗生长发育的生态毒性.环境科学,32(8):2430-2435.
刘延利,潘响亮.2011.抗生素在土壤、黏土矿物中的吸附研究进展.地球与环境,39(4):579-584.
律泽,胡筱敏,安婧,等.2014.佳乐麝香和镉复合污染对土壤中放线菌数量的影响.生态学杂志,33(6):1501-1507.
马莎.2013.四环素和重金属(Cu2+、Cd2+)单一及复合污染对小白菜的毒性效应研究(硕士学位论文).成都:四川农业大学.
王冉,刘铁铮,王恬.2006.抗生素在环境中的转归及其生态毒性.生态学报,26(1):265-270.
王盼亮,张昊,王瑞飞,等.2017.抗生素暴露对小白菜幼苗生长及内生细菌的影响.农业环境科学学报,36(9):1734-1740.
张晶.2015.土霉素暴露对水培小麦生长及根际微生物的影响.湖北农业科学,54(8):1832-1835.
赵英永,戴云,崔秀明,等.2006.考马斯亮蓝G-250染色法测定草乌中可溶性蛋白质含量.云南民族大学学报:自然科学版,15(3):235-237.
周启星,孔繁祥,朱琳.2004.生态毒理学.北京:科学出版社.
朱峰,苏丹,安婧,等.2017.磺胺类抗生素在土壤-植物系统中的迁移特征.生态学杂志,36(5):1402-1407
An J,Chen HW,Wei SH,et al.2015.Antibiotic contamination in animal manure,soil,and sewage sludge in Shenyang,northeast China.Environmental Earth Sciences,74:5077-5086.
An J,Zhou QX,Sun YB,et al.2009.Ecotoxicological effects of typical personal care products on seed germination and seedling development of wheat(Triticum aestivum).Chemosphere,76:1428-1434.
Broderius SJ,Kahl MD,Elonen GE,et al.2005.A comparison of the lethal and sublethal toxicity of organic chemical mixtures to the fathead minnow(Pimephales promelas).Environmental Toxicology&Chemistry,24:3117-3127.
Cleuvers M.2003.Aquatic ecotoxicity of pharmaceuticals including the assessment of combination effects.Toxicology Letters,142:185-194.
Hu XG,Zhou QX,Luo Y.2010.Occurrence and source analysis of typical veterinary antibiotics in manure,soil,vegetables and groundwater from organic vegetable bases,northern China.Environmental Pollution,158:2992-2998.
Migliore L,Godeas F,Filippis SP,et al.2010.Hormetic effect(s)of tetracyclines as environmental contaminant on Zea mays.Environmental Pollution,158:129-134.
Wu YX,Tiedemann AV.2002.Impact of fungicides on active oxygen species and antioxidant enzymes in spring barley(Hordeum vulgare L.)exposed to ozone.Environmental Pollution,116:37-47.
Zhang QQ,Ying GG,Pan CG,et al.2015.Comprehensive evaluation of antibiotics emission and fate in the river basins of China:Source analysis,multimedia modeling,and linkage to bacterial resistance.Environmental Science&Technology,49:6772-6782.
Zikic RV,Stajn A,Saicic ZS,et al.1996.The activities of superoxide dismutase,catalase and ascorbic acid content in the liver of goldfish(Carassius auratus gibelio Bloch.)exposed to cadmium.Physiological Research,45:479-481.