HPLC-ABTS-DAD-Q-TOF/MS在线检测大黄抗氧化活性成分
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摘要
目的:建立大黄特征化学成分和抗氧化活性相关联的二维指纹图谱,研究大黄抗氧化活性物质。方法:利用高效液相多检测器联用的抗氧化活性成分在线检测体系,对大黄中化学成分进行检测,共鉴定出大黄中化学成分15种;其中8种具有抗氧化活性;然后采用清除效率为指标对各活性成分的抗氧化活性进行评价。结果:结果发现化合物葡萄糖紫丁香酸、腺嘌呤、没食子酸、儿茶素或表儿茶素、双花母草素、2-O-桂皮酰-没食子酰葡萄糖等具有较强的清除ABTS~(·+)的活性,而蒽醌类成分对ABTS~(·+)的清除作用较弱。结论:采用HPLC-ABTS-DAD-Q-TOF/MS对大黄中的抗氧化活性成分进行快速分析鉴定,初步阐明大黄在抗氧化环节起作用的效应物质。
OBJECTIVE To establish a two-dimensional fingerprint of Rhei Radix et Rhizoma regarding characteristic chemical composition and antioxidant activity to study its antioxidant activity. METHODS The chemical components of Radix et Rhizoma Rhei were detected by the on-line detection system of antioxidant active ingredients, and a total of 15 chemical components in Radix et Rhizoma Rhei were identified; 8 of them had antioxidant activity; then the antioxidant activity of each active ingredient was evaluated using scavenging efficiency as an indicator.RESULTS The data showed that gluco-syringic acid, adenine, gallic acid, catechin or epicatechin, biflorin, 2-O-cinnamoyl-glucogallin had stronger antioxidant activity, while anthraquinones had lower antioxidant activity. CONCLUSION HPLC-ABTS-DAD-Q-TOF/MS was used for rapid analysis and identification of antioxidant active components in Radix et Rhizoma Rhei, which preliminarily elucidated the effect of Radix et Rhizoma Rhei on antioxidant.
OBJECTIVE To establish a two-dimensional fingerprint of Rhei Radix et Rhizoma regarding characteristic chemical composition and antioxidant activity to study its antioxidant activity. METHODS The chemical components of Radix et Rhizoma Rhei were detected by the on-line detection system of antioxidant active ingredients, and a total of 15 chemical components in Radix et Rhizoma Rhei were identified; 8 of them had antioxidant activity; then the antioxidant activity of each active ingredient was evaluated using scavenging efficiency as an indicator.RESULTS The data showed that gluco-syringic acid, adenine, gallic acid, catechin or epicatechin, biflorin, 2-O-cinnamoyl-glucogallin had stronger antioxidant activity, while anthraquinones had lower antioxidant activity. CONCLUSION HPLC-ABTS-DAD-Q-TOF/MS was used for rapid analysis and identification of antioxidant active components in Radix et Rhizoma Rhei, which preliminarily elucidated the effect of Radix et Rhizoma Rhei on antioxidant.
引文
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[2] Borges G,Degeneve A,Mullen W,et al.Identification of flavonoid and phenolic antioxidants in black currants,blueberries,raspberries,red currants and cranberries [J].J Agr Food Chem,2010,58 (7):3901-3909.
[3] Gao X,Wang Y,Zhu DN,et al.On-line Scavenging Activity of Xiaoaiping Injection for ABTS·+ Radicals by HPLC [J].Chin J Exp Tradit Med Form (中国实验方剂学杂志),2012,18 (5):54-57.
[4] Koleva II,Niederlander HA,van Beek TA.Application of ABTS radical cation for selective on-line detection of radical scavengers in HPLC eluates [J].Anal Chem,2001,73:3373-3381.
[5] Miller NJ,Rice-evans CA.A novel method for mearing antioxidant capacity and its application to monitoring the antioxidant status in premature neonates [J].Clin Sci,1993,84:407-412.
[6] Shi PY,Zhasng YF,Fan XH,et al.Systematic characterization of secondary metabolites from Ixeris sonchifolia by the combined use of HPLC-TOF/MS and HPLC-ITMS [J].Phytochem Anal,2011,22:66-73.
[7] Yang WH,Du ZX.A research for the regularity of N-glycosidic bond cleavage in adenosine derivatives [J].Chin J Anal Lab (分析实验室),2010,29:118-121.
[8] Liu GQ,Dong J,Wang H,et al.ESI fragmentation studies of four tea catechins [J].Chem J Chinese U(高等学校化学学报),2009,30 (8):1566-1570.
[9] Li WX,Li YF,He RR.Review of anti-oxidative evaluation methods for catechins and therapeutic mechanism of catechins [J].Tradit Chin Drug Rese Pharmaco(中药新药与临床药理),2016,27 (2):295-303.
[10] Yang SJ,Ryu JH,Jang DS,et al.A sensitive LC-MS/MS method for the quantitative determination of biflorin in rat plasma and its application to pharmacokinetic studies [J].J Pharm Biomed Anal,2015,115:272-276.
[11] Liu YJ,Wang Q,Jiang M,et al.Screening of effective components for inhibition of tyrosinase activity in rhubarb based on spectrum-efficiency-structure-activity relationship [J].Chin Tradit Herbal Drugs(中草药),2012,43 (11):2120-2126.
[12] Sun H,Zhu C,Zhang HY,et al.Comparative analysis of main constituents of Radixet Rhizoma Rhei and processed Radix et Rhizoma Rhei by HPLC-ESI-TOF-MS [J].Chin Tradit Pat Med(中成药),2009,31 (3):420-424.
[13] Gao LL.Studies on the chemical constituents and biological activity of Rheum tanguticum Maxim.ex Balf.,Rheum officinale Baill.and Rheum palmatum L[D].Beijing:A dissertation submitted for the doctorate of Peking Union Medical College(北京协和医学院博士学位论文),2012.
[14] Li LY,Wang Y,Liu MJ,et al.Analysis of Chemical Components in Zhizi Jinhua Wan by UPLC-Q-TOF-MS /MS [J].Chin J Exp Tradit Med Form (中国实验方剂学杂志),2017,23 (14):1-11.
[15] Luo Z,Li XZ,Ou Yang NN.Chemical conversion of natural antioxidants during extraction[J].Nat Prod Res Dev(天然产物研究与开发),2008,20:376-381.