基于白色念珠菌体外抑菌效果的复方苦参洗剂抑菌作用评价
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摘要
观察复方苦参洗剂中君、臣、佐各药组联合抑菌性质以及协同增效作用。采用加热回流水提法提取各药组获得相应提取液,采用二倍稀释法测得相应各药组单用及联合对白色念珠菌的最低抑菌浓度(ρ_(MIC)),采用联合抑菌指数(I_(FIC))判定两药组联合抑菌作用性质。结果显示,8味中药水提液的ρ_(MIC)范围为7.50～30.00 mg/mL,君、臣、佐各药组的ρ_(MIC)范围为4.01～21.00 mg/mL,复方组ρ_(MIC)为2.22 mg/mL,黄柏+苦参、黄柏+白矾、花椒+白矾的I_(FIC)≤0.5,黄柏+花椒、苦参+花椒、苦参+白矾3组0.5 The combined antifungal properties and synergistic effects of monarch drug, ministerial drug and adjuvant drug of Compound Sophora Flavescens Radix lotion on Candida albicans in vitro were observed in this paper. The extract solution of each group of Compound Sophora Flavescens Radix lotion was obtained by heating backflow water extraction method, the minimal inhibitory concentration(ρ_(MIC))of each group alone and in combination on Candida albicans was measured by double dilution method, using the fraction inhibitory concentration index(I_(FIC)) to determine the combined effects of two traditional Chinese medicines(TCMs). Results showed that the ρ_(MIC) range of 8 kinds of TCM water extraction was 7.50～30.00 mg/mL, the ρ_(MIC) range of monarch drug, ministerial drug and adjuvant drug was 4.01～21.00 mg/mL, the ρ_(MIC) of compound group was 2.22 mg/mL. The I_(FIC) of Phellodendri Chinensis Cortex+Sophorae Flavescentis Radix, Phellodendri Chinensis Cortex+Alumen and Zanthoxyli Pericarpium+Alumen were less than or equal to 0.5, the I_(FIC) of Phellodendri Chinensis Cortex+Zanthoxyli Pericarpium, Sophorae Flavescentis Radix+Zanthoxyli Pericarpium and Sophorae Flavescentis Radix + Alumen were greater than 0.5 and less than or equal to 1. The I_(FIC) of monarch drug+ministerial drug was 0.375 0, the I_(FIC) of monarch drug+adjuvant drug was 0.750 0,the I_(FIC) of ministerial drug+adjuvant drug was 1.000 0.The study showed that the combined antifungal effect of Compound group and monarch group were stronger than that of single herbs. The 4 kinds of monarch drug of Phellodendri Chinensis Cortex, Sophorae Flavescentis Radix, Zanthoxyli Pericarpium and Alumen have strong antifungal activity against Candida albicans. The enhancement of the antifungal effect of compound Sophorae Flavescentis Radix Lotion was related to the synergy and addition of the antifungal effect of each drug group.
引文
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[4]李钰乐,方毅,李亚婷,等.秦皮等10种中药对白色念珠菌抑制作用的体外实验研究[J].延安大学学报(医学科学版),2017,15(2):53-55.DOI:10.3969/j.issn.1672-2639.2017.02.017.
[5]吕正涛,牟子君.中药复方制剂的体外抗菌活性研究[J].中医药学报,2013,41(4):72-75.DOI:10.3969/j.issn.1002-2392.2013.04.025.
[6]宫毓静,刘红,冯淑怡,等.牡丹皮等10种中药对白色念珠菌浮游菌和生物膜作用的研究[J].中国实验方剂学杂志,2011,17(23):129-132.DOI:10.3969/j.issn.1005-9903.2011.23.039.
[7]王文花,李劲松,肖晴晴,等.5种中药体外抗白色念珠菌的实验研究[J].广西中医学院学报,2010,13(3):54-55.DOI:10.3969/j.issn.1008-7486.2010.03.031.
[8]李治建,周凡,窦勤,等.苦参碱体外抗念珠菌活性及对白色念珠菌细胞膜生物合成的影响[J].新疆医学,2018,48(6):603-606.
[9]徐云辉,黄浩,郭兆霞,等.地肤子抗真菌化学成分研究[J].中成药,2012,34(9):1726-1729.DOI:10.3969/j.issn.1001-1528.2012.09.024.
[10]宫毓静,刘红,冯淑怡,等.牡丹皮等10种中药对白色念珠菌浮游菌和生物膜作用的研究[J].中国实验方剂学杂志,2011,17(23):129-132.DOI:10.3969/j.issn.1005-9903.2011.23.039.
[11]张超,雷春娟,魏琴,等.复方中药提取物抗白色念珠菌活性及其主要成分[J].湖北农业科学,2011,50(10):2000-2002.DOI:10.3969/j.issn.0439-8114.2011.10.017.
[12]许懿.小檗碱与氟康唑协同抗耐药白念珠菌作用的蛋白质组学研究[D].上海:第二军医大学,2007.
[13]宋丽雅,倪正,樊琳娜,等.花椒抑菌成分提取方法及抑菌机理研究[J].中国食品学报,2016,16(3):125-130.DOI:10.16429/j.1009-7848.2016.03.017.